• Biotinylated Concanavalin A has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. (vectorlabs.com)
  • Finally, the biofunctionalization of the sensor surface was studied using the layer-by-layer method to deposit different layers of polyelectrolytes and the lectin concanavalin A as the bioreceptor obtaining a limit of detection of 100 cells/mL in just 60 minutes. (upc.edu)
  • Concanavalin A (Con A) is a plant lectin that is purified from jack beans. (bioinfor.me)
  • Proteins are separated by gel electrophoresis before being transferred to a blotting matrix whereupon posttranslational modifications are detected by specific substrates (cholera toxin, concanavalin, phosphomolybdate, etc.) or antibodies. (wikipedia.org)
  • The main objective of this study is to develop a biosensor using IDEA impedimetric transducers for the detection of cyanobacteria in liquid samples. (upc.edu)
  • Desarrollar un biosensor, mediante el uso de transductores impedimétricos del tipo IDEA para la detección de cianobacterias en muestras líquidas. (upc.edu)
  • Labib, M., Hedström, M. , Amin, M., Mattiasson, B. A novel competitive capacitive glucose biosensor based on concanavalin A-labeled nano-gold colloids assembled on a polytyramine-modified gold electrode. (lu.se)
  • Lymphocyte function should be assessed by measuring responses to phytohemagglutinin, a nonspecific stimulant of T-cell proliferation, concanavalin A directed at T-cell proliferation, and pokeweed mitogen directed at T-cell and B-cell proliferation. (medscape.com)
  • A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. (wikipedia.org)
  • A western blot is used for the detection of specific proteins in complex samples. (wikipedia.org)
  • The eastern blot is used for the detection of specific posttranslational modifications of proteins. (wikipedia.org)
  • The far-eastern blot is for the detection of lipid-linked oligosaccharides. (wikipedia.org)
  • The northern blot is for the detection of specific RNA sequences in complex samples. (wikipedia.org)
  • Proteins are first separated by size using electrophoresis before being transferred to an appropriate blotting matrix (usually polyvinylidene fluoride or nitrocellulose) and subsequent detection with antibodies. (wikipedia.org)
  • 1973. Carcinogens are mutagens: A simple test system combining liver homogenates for activation and bacteria for detection. (cdc.gov)
  • A concanavalin-A-induced suppressor cell assay was carried out in 10 patients with chronic filarial disease, 13 asymptomatic microfilaria carriers and 7 healthy subjects. (karger.com)
  • Ovalbumin was used as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophils. (yunbangpharm.com)
  • Which of the following molecular diagnostic techniques is very useful for early detection? (examside.com)
  • The advantages of using this kind of data as a management tool would be to allow the early detection of metabolic or management problems. (ac.be)
  • Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. (wikipedia.org)
  • 9. Khaspekova SG, Vlasik TN, Byzova TV, Vinogradov DV, Berndt MC, Mazurov AV. (1993) Detection of an epitope specific for the dissociated form of glycoprotein IIIa of platelet membrane glycoprotein IIb-IIIa complex and its expression on the surface of adherent platelets. (yoproteins.eu)
  • Description: Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. (gentaurtop.com)
  • BioMag Concanavalin A has moreover been used for CUT&RUN, a chromatin profiling protocol that has numerous key advantages over ChIP. (isotope.info)
  • This work addresses contaminant detection both in clinical situations and during protein purification of pharmaceutical products. (lu.se)
  • incubation of mycobacterium bovis-sensitized bovine peripheral blood lymphocytes with concanavalin a or m. bovis purified protein derivative and indomethacin caused a consistent, statistically significant increase in [3h] thymidine uptake as compared to cultures without indomethacin. (liverpool.ac.uk)
  • Upon peptidoglycan detection, Nod1 and Nod2 recruit and associate with the adaptor protein Rip2, triggering proinflammatory pathways such as NF- κ B and the mitogen-activated protein (MAP) kinases p38, JNK, and ERK [ 4 ]. (hindawi.com)
  • Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. (ppjonline.org)
  • this method was useful for the detection of active tuberculosis with open lesions. (liverpool.ac.uk)
  • The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. (ppjonline.org)
  • On day +13 animals were euthanized, draining lymph nodes were excised, and mRNA was isolated immediately or following 24 or 48 h of culture in the presence or absence of concanavalin (Con) A. Differential expression of cytokine mRNA was most notable following 24 h incubation with Con A. Modulation of IL-4, -10, and IFN-gamma following chemical exposure was consistent with previous studies. (cdc.gov)
  • 4-Nitrophenyl-α-D- glucopyranoside is also used for the detection of glucansucrases and for yeast alpha-D-glucosidase and for crystal structure studies of the complexes of concanavalin A. (goldbio.com)
  • Los clientes que adquieran múltiples kits pueden optar por ahorrar espacio de almacenamiento retirando el embalaje del kit y recibiendo los componentes de sus ensayos multiplex en bolsas de plástico para un almacenamiento más compacto. (merckmillipore.com)
  • When splenic lymphocytes were exposed to CP in the presence of induced S9 mix, a dose related inhibition of the response to the B-cell mitogen, lipopolysaccharide (LPS), and to the T-cell mitogen, concanavalin A (Con A), was observed. (nih.gov)
  • These activators include the following: concanavalin A, lipopolysaccharide, phorbol esters plus calcium ionophore or ionomycin, phytohaemaglutinin, staphylococcus enterotoxin B, and monoclonal antibodies directed against subunits of the TCR/CD3 complex (with or without antibodies directed against costimulatory receptors, such as CD28). (bdbiosciences.com)
  • 15. Label-free electrochemiluminescence biosensor for ultrasensitive detection of telomerase activity in HeLa cells based on extension reaction and intercalation of Ru(phen)3 (2. (nih.gov)
  • 19. Electrogenerated chemiluminescence aptasensor for ultrasensitive detection of thrombin incorporating an auxiliary probe. (nih.gov)
  • Based on the highly specific interaction between concanavalin A (Con A) and glucose (Glu), a competitive electrochemiluminescence (ECL) biosensor was constructed for ultrasensitive detection of Con A. Nanocomposites with excellent electrocatalytic and photothermal properties were obtained by covalently bonding zinc oxide quantum dots (ZnO QDs) to vanadium carbide MXene (V2C MXene) surfaces. (bvsalud.org)
  • To achieve a stable and robust sensing system the anchor chain length and the density of the assembled carbohydrates on the gold particle surface has been examined to determine the optimal coverage for maximal aggregation with both RCA 120 and Concanavalin A (Con A) lectins. (rsc.org)
  • On day +13 animals were euthanized, draining lymph nodes were excised, and mRNA was isolated immediately or following 24 or 48 h of culture in the presence or absence of concanavalin (Con) A. Differential expression of cytokine mRNA was most notable following 24 h incubation with Con A. Modulation of IL-4, -10, and IFN-gamma following chemical exposure was consistent with previous studies. (cdc.gov)
  • Furthermore, proinflammatory cytokine release induced by concanavalin A was significantly decreased in NOX2 −/− compared with WT splenocytes. (diabetesjournals.org)
  • The most critical step for detection of intracellular accumulations of cytokines by intracellular staining is the isolation and activation of a cell population to induce production of the cytokine of interest. (bdbiosciences.com)
  • The specificity of the detection is demonstrated by a comparison with the corresponding polypeptide profile obtained by silver nitrate staining of the gel. (pucv.cl)
  • A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. (wikipedia.org)
  • 12. "Off-On"switching electrochemiluminescence biosensor for mercury(II) detection based on molecular recognition technology. (nih.gov)
  • Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future. (heraeus-targets.com)
  • 6. Electrogenerated chemiluminescence biosensor incorporating ruthenium complex-labelled Concanavalin A as a probe for the detection of Escherichia coli. (nih.gov)
  • Proteins are first separated by size using electrophoresis before being transferred to an appropriate blotting matrix (usually polyvinylidene fluoride or nitrocellulose) and subsequent detection with antibodies. (wikipedia.org)
  • Proteins are separated by gel electrophoresis before being transferred to a blotting matrix whereupon posttranslational modifications are detected by specific substrates (cholera toxin, concanavalin, phosphomolybdate, etc.) or antibodies. (wikipedia.org)
  • Antibodies to Y. enterocolitica were raised for rapid Yersinia detection in the stool. (who.int)
  • A highly sensitive and simple method to enhance detection of glycoproteins resolved by either one- or two-dimensional polyacrylamide gel electrophoresis is described. (pucv.cl)
  • 80, 69-82) that uses concanavalin A conjugated with fluorescein isothyocyanate to detect the carbohydrate moiety of glycoproteins. (pucv.cl)
  • In vitro immunotoxicological assays for detection of compounds requiring metabolic activation. (nih.gov)
  • 14. Electrogenerated chemiluminescence detection of mercury(II) ions based on DNA probe labeled with ruthenium complex. (nih.gov)
  • Farka , V., Ait Mohand, F., Stratilov , E. Sensitive detection of transglycosylating aktivity of xyloglucan endotransglycosylase/hydrolase (XTH) after isoelectric focusing in polyacrylamide gels. (chem.sk)
  • Treatment of control cells with concanavalin A stimulated MMP-dependent pro-MMP-2 activation and accumulation of M(r) 55,000 and 43,000 forms of MT1-MMP in cell extracts. (nih.gov)
  • Addition of either the MMP inhibitor GM-6001-X or exogenous tissue inhibitor of metalloproteinase-2 to concanavalin A-treated cells resulted in loss of the M(r) 43,000 form of MT1-MMP and accumulation of the M(r) 55,000 form of the enzyme in cell extracts, suggesting that the M(r) 43,000 form is a product of MMP-dependent M(r) 55,000 MT1-MMP proteolysis. (nih.gov)
  • The abundance of early antigens in cells treated with Arildone was evidence for expression of the viral genome and this was confirmed by detection of immediate-early viral proteins in the presence of the drug. (microbiologyresearch.org)
  • In the article " Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. Albicans " Anne-Céline Kohler, Leonardo Venturelli, Abhilash Kannan, Dominique Sanglard, Giovanni Dietler, Ronnie Willaert and Sandor Kasas, explore the effect of fibronectin on the nanomotion pattern of different Candida albicans strains by atomic force microscopy ( AFM )-based nanomotion detection and correlated the cellular oscillations to the yeast adhesion onto epithelial cells. (nanosensors.com)
  • A western blot is used for the detection of specific proteins in complex samples. (wikipedia.org)
  • The eastern blot is used for the detection of specific posttranslational modifications of proteins. (wikipedia.org)
  • The northern blot is for the detection of specific RNA sequences in complex samples. (wikipedia.org)
  • specific for detection of FGF2 were used in accordance with Jyo-Oshiro et al. (vegfr-1inhibitor.com)
  • The authors demonstrate the ability of nanomotion detection to monitor in real time and in a label-free manner cellular activity changes induced by interacting ligands. (nanosensors.com)
  • I am currently working on MRI methods for direct detection and mapping of neuronal activity, bypassing hemodynamic contrast altogether. (nih.gov)
  • Its rapid detection is highly desirable. (rsc.org)
  • In this way the relatively sophisticated pattern analysis algorithms for edge detection, structure skeletonizing, and densitometry can concentrate on small important subsets of the large amount of raw data involved. (nih.gov)
  • To develop a simple immunoassay for the detection of galactomannan (GM), an IA biomarker. (microbiologyresearch.org)
  • The present study aims to develop a concanavalin A (ConA) coated chitosan (CS) nanocarrier-based drug delivery for the targeted release of peptides to the site of H. pylori infection. (bvsalud.org)
  • Algorithms which may be used for automated computer analysis of the images include dynamic threshold and maximum gradient techniques for edge detection. (nih.gov)
  • His Concanavalin A study integrates concerns from other disciplines, such as Molecule, Receptor, Protein subunit and Binding site. (research.com)
  • Further investigations of these cytokines may provide additional insight into the mechanisms of chemically induced respiratory sensitization and provide endpoints for the detection of a chemical's ability to elicit IgE-mediated hypersensitivity responses. (cdc.gov)
  • 1973. Carcinogens are mutagens: A simple test system combining liver homogenates for activation and bacteria for detection. (cdc.gov)
  • 16. Reaction-based turn-on electrochemiluminescent sensor with a ruthenium(II) complex for selective detection of extracellular hydrogen sulfide in rat brain. (nih.gov)
  • Upon interrogation by an optical system, the amplification system produces a signal capable of detection external to the skin of the patient. (justia.com)