Detection cells localization of specific rnas. Medical search. Frequent questions

Various studies have shown that the cell-cycle-related regulatory proteins UBE2C, PLK1, and BIRC5 promote cell proliferation and migration in different types of cancer. (bvsalud.org)
SNAP-tag, CLIP-tag™ and cell surface-specific ACP/MCP-tag systems can specifically label subpopulations of target proteins expressed on the cell surface using non-cell permeable substrates (3). (neb.com)
Read about the NEB's set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins. (neb.com)
Each image shows the proteins in a different plane of the cell starting from the top, and the magnified images on the bottom compare the resolution achieved with SDC-PAINT (left) to that possible with conventional confocal microscopy (right). (scitechdaily.com)
However, although the researchers demonstrated DNA-PAINT's potential by visualizing single biomolecules such as proteins in fixed cells at a fixed close distance, the technology could not yet investigate molecules deep inside of cells. (scitechdaily.com)
The MPI/Wyss Institute team demonstrates that the method can visualize a variety of different molecules, including combinations of different proteins, RNAs, and DNA throughout the entire depth of whole cells at super-resolution. (scitechdaily.com)
The team mapped out the presence of different combinations of proteins within whole cells, and then went beyond that. (scitechdaily.com)
By diversifying our labeling approaches, we also visualized different types of individual biomolecules in the chromosome-containing nucleus, including sequences in the DNA, proteins bound to DNA or the membrane that encloses the nucleus, as well as nuclear RNAs," adds Yin, who is also co-leader of the Wyss Institute's Molecular Robotics Initiative, and Professor of Systems Biology at Harvard Medical School. (scitechdaily.com)
Wider perspectives can be acquired by tracking the dynamic cell-to-cell variations in proteins. (genengnews.com)
They can detect everything from DNA and RNA to proteins and lipids, allowing researchers to investigate a vast array of cellular functions. (dadangoray.com)
Proteins: Fluorochrome can detect specific proteins in cells or tissues, allowing researchers to study their distribution and expression levels. (dadangoray.com)
Long-term memory consolidation: The role of RNA-binding proteins with prion-like domains. (tcd.ie)
Proteins represent the functional units of the cell. (cmmc-uni-koeln.de)
Production of functional proteins also requires the correct flow of information from DNA into RNA and finally into protein. (cmmc-uni-koeln.de)
The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). (antibodies-online.com)
At the sub-cellular scale, maps document the spatial organization of proteins, RNA, DNA, and metabolites with nanometer precision and temporal acuity on the order of seconds. (stanford.edu)
Conclusion】 The expression trends of ADAMTS 1, PI 3 K and A k t genes and proteins were consistent and showed a positive correlation in ovary and granulosa cells of Mongolian sheep,suggested that ADAMTS 1 gene played an important role in twin birth of Mongolian sheep mediated by PI3K/Akt signaling pathway. (chvm.net)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
Small angle neutron scattering (SANS) provides unique insight into biomacromolecular complexes by combining solvent contrast variation (H2O:D2O exchange) with either natural contrast between different classes of biomolecules (proteins, RNA/DNA, lipids/detergents) and/or by applying artificial contrast, i.e. deuteration of specific biomolecules. (lu.se)

A patent in situ hybridization technique his lab developed for detecting RNA in morphologically preserved cells revealed that messenger RNA can localize in specific cellular compartments. (wikipedia.org)
NEW YORK ─ Bio-Techne and Leica Biosystems said Tuesday that they have received the CE mark for Bio-Techne's RNAscope In Situ Hybridization Detection Kit for automation on the Leica Biosystems Bond-III staining platform. (genomeweb.com)
The life sciences firm's RNAscope In Situ Hybridization Detection Kit and probes identify RNA expression patterns and localization at the single-cell level with spatial and morphologic context. (genomeweb.com)
Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
Fluorescence in situ hybridization fish is an effective technique that enables direct visualization of genetic alterations in the cell. (web.app)
Fluorescence in situ hybridization fish is a cytogenic technique used for the detection and localization of rna sequences within tissues or cells 116. (web.app)
Fluorescence in situ hybridization fish preparation of fish probe recommended filter set fish is a technique used to identify and localize the presence or absence of specific dna sequences on cells and tissues. (web.app)
It has emerged as a powerful cytogenetic method for the analysis of cells and tissues on the transcriptome and genome level with more than 6 publications in the literature per day since the late 1990s fig. Fluorescence in situ hybridization fact sheet itrc. (web.app)
Fluorescence in situ hybridization fish is a cytogenetic technique used to detect the presence or absence and location of specific gene sequences. (web.app)
We detected TSPyV RNA in tissue specimens by in situ hybridization, which revealed localization to endothelial cells. (cdc.gov)
For negative in situ hybridization controls, we used tissue specimens from patients with Merkel cell polyomavirus-positive Merkel cell carcinoma and BK polyomavirus nephropathy and a T-cell lymphoma tissue microarray that included 1 case of Merkel cell polyomavirus-positive T-cell lymphoma. (cdc.gov)
The probes are great for in situ hybridization and cell or tissue imaging applications requiring a short probe, such as in the detection of short sequence mutations or for discriminating between closely related sequences such as splice variants and isoforms. (qiagen.com)
In general, spatial resolution transcriptomics is mainly divided into two categories: One is an image-based method based on in situ hybridization, in which staining and barcoding RNA in its original position are the key points to achieve multiple detection. (encyclopedia.pub)
Image-based methods originating from single-molecule RNA in situ hybridization are mainly divided into fluorescence in situ hybridization and fluorescence in situ sequencing. (encyclopedia.pub)
Fluorescence in situ hybridization uses labeled fluorescent probes to determine RNA/DNA abundance in tissues or cells, and the first example of imaging a single RNA species can be attributed to single-molecule fluorescence in situ hybridization (smFISH) [ 12 ] , which provides absolute quantification of the copy number and localization of RNA molecules in cells. (encyclopedia.pub)

Dysregulation of circular RNAs (circRNAs) has recently been found to play an important role in the progression and development of cancers such as non-small cell lung cancer (NSCLC). (biomedcentral.com)
Circular RNAs (circRNAs) belong to a class of noncoding and protein-coding RNAs localized to the cytoplasm which originate from both single and multiple exons [ 7 , 8 ]. (biomedcentral.com)
Circular RNAs (circRNAs) are a form of noncoding and closed loop RNA molecules and play vital roles in the progression of various types of cancer in humans. (hindawi.com)
Circular RNAs (circRNAs) are molecular sponges that are involved in regulation of multiple types of cancer. (spandidos-publications.com)
Circular RNAs (circRNAs) are closed, long, non-coding RNAs that are formed via direct reverse splicing of precursor mRNA that function by regulating RNA transcription and protein production, as well as sponging miRNAs ( 4 ). (spandidos-publications.com)

Long non-coding RNAs (lncRNAs) are widely recognized as important regulators of gene expression. (mdpi.com)
We determined whether cucurbitacin I modulates M2-like polarization in macrophages in vitro and conducted RNA sequencing to identify gene expression changes induced by cucurbitacin I in macrophages. (bvsalud.org)
In this investigation, comparative gene expression and electron microscopy studies were used to address germ cell formation in Oikopleura dioica ( O. dioica ). (biomedcentral.com)
PEM-1 has a role in repressing gene expression in the germ line precursor cells during germ-soma segregation [ 6 , 7 ]. (biomedcentral.com)
RNA: Fluorochrome can detect RNA molecules, allowing researchers to study gene expression at the transcriptional level. (dadangoray.com)
Counts are usually applicable for analysis of differential gene expression from RNA-seq data (Anders 2013). (buyresearchchemicalss.net)
Although a high level of PDE10A gene expression is observed in the testis, information on the identity of the isoforms or on the cell type that express the PDE10 protein is lacking. (plos.org)
Meanwhile, it has unique advantages in the analysis of specific gene expression and cell localization and can provide key information for the research of detailed molecular mechanisms. (encyclopedia.pub)
The mouse pS2 gene is highly expressed in the stomach mucosa cells, whereas no pS2 gene expression could be detected in the mouse mammary gland, even during postnatal development processes dependent on growth factors or hormones. (xenbase.org)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

High ERV-K env total protein expression of all tumor subtypes significantly correlated with low tumor grading and a longer disease specific survival using multivariable analyses. (frontiersin.org)
Once the fluorophore is coupled to the desired protein, the label fluorescesces permitting visualization in living or fixed cells. (neb.com)
This approach permits discrimination of different populations of a cell surface protein: those properly translocated to the plasma membrane from those retained in the secretory pathway or already internalized ( e.g. upon ligand binding). (neb.com)
2017). In this dataset, we identified and characterized the Liver-Specific Protein 2 (LISP2) protein as an early molecular marker of liver stage development. (elifesciences.org)
Also in development are approaches that could improve our ability to see patterns of protein localization within the cell. (genengnews.com)
Therefore, identification of low-abundance protein modifications still depends on the availability of specific enrichment strategies. (genengnews.com)
Detection of a protein of avian leukoviruses in uninfected chick cells by RIA. (microbiologyresearch.org)
Repression of Pumilio Protein Expression by Rbfox1 Promotes Germ Cell Differentiation. (tcd.ie)
A genome-wide resource for the analysis of protein localisation in Drosophila. (tcd.ie)
Altered Ribostasis: RNA-Protein Granules in Degenerative Disorders. (tcd.ie)
The Ataxin-2 protein is required for miRNA function and synapse-specific, long-term olfactory habituation. (tcd.ie)
MIPβ1KO islets displayed a significant increase in protein levels of the apoptotic marker cleaved-Poly (ADP-ribose) polymerase and a reduction of the cell cycle marker cyclin D1. (oncotarget.com)
Importantly, protein quality control effectors are also involved in further aspects of proteome balance, such as regulation of protein abundance, subcellular localization, and assembly into macromolecular complexes. (cmmc-uni-koeln.de)
Our research group aims at understanding how protein quality control pathways can shape cell physiology, and how its factors can achieve client specificity given the enormous heterogenicity of the proteome. (cmmc-uni-koeln.de)
Cell signaling: Hormones and their receptors, cell surface receptor, signaling through G-protein coupled receptors, signal transduction pathways, second messengers, regulation of signaling pathways, bacterial and plant two-component signaling systems, bacterial chemotaxis and quorum sensing. (pathfinderacademy.in)
The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. (antibodies-online.com)
It is also thought have a primary role in the formation of specific myometrial protein species in parturition. (antibodies-online.com)
A protein named TDP-43 has been lost from its normal location in the nucleus of the cell in virtually all persons having amyotrophic lateral sclerosis (ALS) and in up to half of all cases of frontotemporal dementia and Alzheimer's disease (AD). (azolifesciences.com)
Some lncRNAs (referred to as long intergenic non-coding RNAs, lincRNAs) are transcribed from separate loci, whereas others are transcribed divergently from promoters of protein-coding genes or in antisense direction to these. (biomedcentral.com)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)
In a first couple of examples, I will show how distance and shape restraints from SANS have helped to improve the uniqueness of structural models for two multi-protein-RNA complexes, in combination with NMR restraints and building blocks from crystallography [1, 2]. (lu.se)

The Stellaris® RNA FISH technology from LGC Biosearch Technologies offers an RNA visualisation method for simultaneous detection, localisation, and quantification of individual mRNA molecules at the cellular level. (bioscience.co.uk)

Currently, a range of probes can be synthesized to locate and quantify specific short RNAs, genes, entire chromosomes, and even cells (Evanko 2007 ). (springer.com)
Our study reveals the potential mechanisms through which cell-cycle-related genes regulate metabolism and proposes a potential combined targeted therapy for patients with higher PLK1 and ACLY expression in pan-cancer. (bvsalud.org)
In most cases, circRNAs usually act as a competitive endogenous RNA (ceRNA) that can absorb miRNAs to regulate the expression of targeted genes [ 9 ]. (hindawi.com)
The non-specific lethal complex regulates genes and pathways genetically linked to Parkinson's disease. (medscape.com)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)

Noncoding RNAs, including circRNAs, microRNAs, and lncRNAs, have become a significant focus of research attention due to their crucial role in regulating the progression of multiple malignancies [ 5 ], including bladder cancer, gastric cancer, nasopharyngeal carcinoma, and pancreatic cancer. (hindawi.com)

Our Custom LNA mRNA Detection Probes are designed with optimal LNA positioning to achieve high sequence specificity, low secondary structure and minimal self-annealing. (qiagen.com)
Our user-friendly online design tool lets you easily design highly sensitive LNA probes for your specific mRNA sequence of interest. (qiagen.com)
These miRNAs which targeted β -catenin mRNA were confirmed by dual-luciferase reporter system and RNA-pulldown. (hindawi.com)
Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7. (xenbase.org)
The present study aimed to screen and explore the key circRNA/microRNA (miRNA or miR)/mRNA interactions in head and neck squamous cell carcinoma (HNSCC) using bioinformatics. (spandidos-publications.com)
Thus, further investigations into the specific molecular mechanisms underlying circRNA/miRNA/mRNA regulation are required to aid discovery of effective therapeutic targets. (spandidos-publications.com)

His lab has shown that the dynamics of RNA transcription on a single gene can be interrogated by live cell imaging, as well as by multiplexed fluorescent probes. (wikipedia.org)
What are the Differences Between DNA and RNA Probes? (enzolifesciences.com)
Specific molecular probes and primers are designed for this purpose. (enzolifesciences.com)
In practice, double and single stranded DNAs, mRNAs, and other RNAs synthesized in vitro are all used as probes. (enzolifesciences.com)
Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, and synthetic oligonucleotide probes. (enzolifesciences.com)
Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)
By confining FISH probes at the tip of this microfabricated scanning probe, we locally exposed approximately 1000 selected MCF-7 cells of a monolayer to perform incubation of probes - the rate-limiting step in conventional FISH. (springer.com)
The continuous flow of probes and shaping liquid on these selected cells resulted in a 120-fold reduction of the hybridization time compared with the standard protocol (3 min vs. 6 h) and efficient rinsing, thereby shortening the total FISH assay time for centromeric probes. (springer.com)
We further demonstrated spatially multiplexed μFISH, enabling the use of spectrally equivalent probes for detailed and real-time analysis of a cell monolayer, which paves the way towards rapid and automated multiplexed FISH on standard cytological supports. (springer.com)
It relies on the sequence-specific hybridization of probes to their complementary targets in individual cells, followed by direct or indirect detection of the labelled probe. (springer.com)
a Using a pipette in conventional FISH, CEP7 probes are deposited on the entire cell monolayer. (springer.com)
b In μFISH implemented with an MFP, CEP7 probes are localized on selected cells, and the FISH signal is inspected in real time. (springer.com)
After incubation, the liquid is switched in the MFP head, scanning is performed, and different cells are exposed to CEP17 probes. (springer.com)
Multiplexed FISH is often used to locate multiple targets in a cell nucleus simultaneously using probes of different colors, in order to study sparse cytological substrates (Lichter 1997 ). (springer.com)
Off-target binding of single probes generates only weak and diffuse fluorescence, which falls well below the threshold for detection of the targeted mRNAs. (bioscience.co.uk)

Fish is a technique used to identify and localize the presence or absence of specific dna sequences on cells and tissues. (web.app)
Oligonucleotides are short sequences of nucleotides (RNA or DNA), typically with twenty or fewer bases. (azolifesciences.com)
Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)

Fluorescence microscopy allows the visualization of fluorescent molecules in cells and tissues under a microscope. (dadangoray.com)

Long non-coding RNAs (lncRNAs) have shown promise as biomarkers in other cancer types and in some cases functionally contribute to tumor development and progression. (biomedcentral.com)
Long non-coding RNAs (lncRNAs) have moved into the focus of cancer research as good candidates for tumor biomarkers and as regulators of various neoplastic cell properties. (biomedcentral.com)
Importantly, many lncRNAs are expressed in a cell type-specific manner and their expression changes during tumorigenesis. (biomedcentral.com)

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. (xenbase.org)

RNAscope technology is highly sensitive and specific due to its double Z-probe design, resulting in a high signal-to-noise ratio of staining in various tissues types and allowing diagnostic pathologists to simultaneously visualize, localize, and quantify biomarker expression, they added. (genomeweb.com)
A dualcolor, dualfusion approach is commonly used and can detect all bcrabl1 fusions with excellent sensitivity and specificity including cytogenetically cryptic translocations fig. Fish is a cytogenetic technique used to detect and localize the presence or absence of specific. (web.app)
In contrast to our hypothesis, we found that TSPyV RNA did not localize to neoplastic cells. (cdc.gov)

Although DNMT3A and DNMT3B are highly homologous, their redundancy seems to be limited, and their unique biological function, e.g., specificity for methylation patterns in distinct cell types, is still elusive 9 . (nature.com)
In this work, we analyze expression patterns of DNMT3A in patients with IBD, perform loss of function experiments using Caco-2 cell line and generate a conditional Dnmt3a ΔIEC mouse line. (nature.com)
The BK polyomavirus nephropathy sections showed weak, orange-red discoloration of tubular epithelium ( Figure , panel L) that was distinguishable from the bright red, granular staining patterns for TSPyV RNA observed in the positive control and tissue specimens from the 3 patients. (cdc.gov)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)

This affinity and complementary sequence allow binding to specific regions of a target sequence of nucleotides. (enzolifesciences.com)

Our ongoing project aims to elucidate how cells recognize temperature or cellular events caused by the temperature changes and control the expression of DESAT1 to adapt the cellular processes and behaviors," Umeda said. (asbmb.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The peptide hormone insulin, produced by pancreatic β-cells, is critical for maintaining normal blood glucose levels and acts to regulate cellular metabolism and growth by facilitating glucose uptake by peripheral tissues. (diabetesjournals.org)
Cellular communication: Regulation of hematopoiesis, general principles of cell communication, cell adhesion and roles of different adhesion molecules, gap junctions, extracellular matrix, integrins, neurotransmission and its regulation. (pathfinderacademy.in)
Beyond the single cell, we also strive to map cellular ensembles, such as brain tissue. (stanford.edu)
We develop tools that can be used to construct cell and tissue atlases, and map the cellular circuits that give rise to function and behavior. (stanford.edu)
To achieve these goals, our laboratory has focused on the development of scalable technologies to detect, measure, and manipulate molecules and circuits, both at the sub-cellular level, and at the level of cell populations. (stanford.edu)
β -Catenin is a transcription factor that plays an important role in cell proliferation, cellular differentiation, apoptosis, and metastasis [ 10 ]. (hindawi.com)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
According to this theory, the differences in phenotypes expressed by mediastinal germ cell tumors (MGCTs) and gonadal germ cell tumors may be explained by differences in the cellular environment between the gonad and the anterior mediastinum. (medscape.com)

Endothelial cells lining blood vessels help keep what belongs in and what doesn't belong out. (asbmb.org)

Reduced circ-EPB41 expression in NSCLC decreased cell proliferation and invasion in both in vitro and in vivo experiments. (biomedcentral.com)
Circ_0002483 has been verified to inhibit NSCLC progression in vivo and in vitro, and increases NSCLC cell sensitivity to Taxol through sponging of miR-182-5p to inhibit expression of FOXO1, FOXO3 and GRB2 mRNAs [ 13 ]. (biomedcentral.com)
Moreover, we found that cucurbitacin I-treated macrophages reduced the migration of cancer cells by inhibiting the M2 polarization in vitro. (bvsalud.org)
The role of β1 integrin in maintaining optimal islet function has previously been identified in vitro using cell lines and isolated islets. (oncotarget.com)

In recent years it became evident that translation plays a fundamental role in the detection of defective mRNAs. (cmmc-uni-koeln.de)

Fluorochrome detection refers to a technique used to identify and quantify specific biomolecules using fluorescent dyes. (dadangoray.com)
In summary, fluorochrome detection is a powerful technique used to identify and quantify specific biomolecules in cells and tissues. (dadangoray.com)

This process involves the release of a subset of maternal transcripts from the centrosome-attracting body (CAB) in the progenitor cells of the germ line. (biomedcentral.com)
We found that the RNA localization pattern of pumilio ( pum1 ) is similar to the pattern described for a subset of maternal transcripts marking the posterior end of ascidian embryos. (biomedcentral.com)
We found no localization of vasa ( vas ) transcripts to any sub-region within the germ-line precursor cells. (biomedcentral.com)
One subset of postplasmic/PEM transcripts, still attached to the CAB, segregate into the endodermal strand cells (B8.11). (biomedcentral.com)
Both the PGCs (B8.12 cells) and the endodermal strand cells (B8.11 cells) inherit these transcripts. (biomedcentral.com)
Both highly sensitive and specific, a Stellaris RNA FISH assay is comprised of up to 48 single-labelled oligonucleotides designed to selectively bind to target RNA transcripts. (bioscience.co.uk)
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. (xenbase.org)

Multi-sample BAM QC analysis of a H2AX ChiP-seq experiment in human cells comparing four different conditions (Koeppel This mode allows computation of metrics specific to RNA-seq data, including per-transcript coverage, junction sequence distribution, genomic localization of reads, 5C3 bias and consistency of the library protocol. (buyresearchchemicalss.net)
Our method thereby provides an effective tool of general utility for the genomic localization of 5fC. (nih.gov)
We also report the development and application of a single-base resolution method for the detection of 5fC in genomic DNA by hydroylamine mediated protection of 5fC from deamination during bisulfite treatment, or 5fC Chemical Assisted Bisulfite Sequencing (fCAB-Seq). (nih.gov)
The cell carrying this abnormality is relatively protected against apoptotic death because of the oncogenic effect of CCND2, leading to re-initiation of cell cycle and genomic instability. (medscape.com)

Taken togeter, these data revealed the important role of circ-EPB41 in regulating NSCLC cell invasion and proliferation by modifying miR-486-3p/eIF5A axis-mediated stemness. (biomedcentral.com)
Cell proliferation and invasion were assayed using the kidney cell lines HEK293 with wild-type p53 and a ccRCC cell line MZ1257RC mutated for p53. (frontiersin.org)
HEK293 cells demonstrated a restriction of ERV-K113 env expression and invasion with no changes in proliferation in the absence of Aza. (frontiersin.org)
Ex vivo exposure of first trimester testes to CBD, THC, or CBD/THC [ratio 1:1] at 10 −7 to 10 −5 M altered testosterone secretion by Leydig cells, AMH secretion by Sertoli cells, and impacted testicular cell proliferation and viability as early as 72 h post-exposure. (biomedcentral.com)
While knockout circIFT80 in HT29 cell or SW480 cells, the proliferation, and migration of the cells were inhibited, the cell cycle was arrested in G2/M phase, and the cell apoptosis was increased. (hindawi.com)
For example, circRNA_000166 can function as an oncogene by promoting the proliferation of CRC cells and by limiting apoptosis. (hindawi.com)
Effects of lncRNA overexpression or downregulation on cell proliferation, clonogenicity, migration and chemosensitivity were studied in HNSCC cell lines. (biomedcentral.com)

Head and neck squamous cell carcinoma (HNSCC) is a common malignancy with ~600,000 cases new each year worldwide. (spandidos-publications.com)
For example, circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis ( 9 ). (spandidos-publications.com)
Few diagnostic and prognostic biomarkers are available for head-and-neck squamous cell carcinoma (HNSCC). (biomedcentral.com)
Analysis of TCGA pan-cancer data revealed significant overexpression of CASC9 across different other entities including bladder, liver, lung and stomach cancers and especially in squamous cell carcinoma (SCC) of the lung. (biomedcentral.com)

In a recent study, Dr. Chen and colleagues developed a quantitative proteomics strategy to stoichiometrically identify site-specific lysine acetylation in mammalian cells. (genengnews.com)

NC_014361.1) and RNAscope 2.5 HD Reagent Kit-RED (Advanced Cell Diagnostics, https://acdbio.com ) to detect TSPyV RNA in cutaneous biopsy specimens. (cdc.gov)
From studying the intricate details of a single cell to unraveling the mysteries of complex biological networks, there is almost nothing that these tiny molecules cannot detect. (dadangoray.com)
Researchers can choose the appropriate fluorochrome based on their experimental needs and the specific biomolecule they want to detect. (dadangoray.com)
Screening for prostate cancer, with prostate-specific antigen (PSA) evaluation and sometimes digital rectal examination (DRE), can detect asymptomatic cases and allow early diagnosis. (medscape.com)
Using custom probe sets enables the researcher to design their own to detect the RNA of interest. (bioscience.co.uk)
have developed a new method to detect RNA viruses based on the triplex-forming probe technology. (azolifesciences.com)

It can visualize specific cytogenetic abnormalities copy number aberrations such as chromosomal deletion, amplification, and translocation. (web.app)
Nucleic Acid Amplification (NAA) test is an example of direct detection. (cdc.gov)
Amplification of CCND2 activates cdk4/6, allowing the cell to progress through the G1-S checkpoint. (medscape.com)

Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
The RNAscope Multiplex Fluorescent assays are ideal for single-molecule detection of multiple RNA targets (up to four targets) simultaneously within the same tissue. (acdbio.com)

One of the challenges in developmental biology is to understand how cells adopt specific characteristics during embryogenesis. (biomedcentral.com)
In their new study, they adapted DNA-PAINT technology to confocal microscopes, which are widely used by researchers in cell biology laboratories to image whole cells and thicker tissues at lower resolution. (scitechdaily.com)
By emitting fluorescent light when exposed to specific wavelengths of light, fluorochromes have become indispensable tools in the fields of biology and medicine. (dadangoray.com)
This technique is widely used in fields such as cell biology, immunology, and microbiology. (dadangoray.com)
Therefore, positioning cells by spatial omics technology can explore the differentiation trajectory and development of cell lineages [ 1 ] [ 2 ] , which may have great application prospects in developmental biology. (encyclopedia.pub)
Appropriate content should focus more on the method or approach rather than disease or biology-specific questions. (ashg.org)
Appropriate content should focus more on the technique rather than disease- or biology-specific questions. (ashg.org)
Many phage have proved useful in the study of molecular biology and as vectors for the transfer of genetic information between cells lambda bacteriophage can also undergo a lytic cycle or can enter a lysogenic cycle, in which the page DNA is incorporated into that of the host, awaiting a signal that initiates events leading to replication of the virus and lysis of the host cell. (genomicglossaries.com)

This technology has implications for understanding the role of RNA in such disease processes as cancer metastasis and intellectual disability. (wikipedia.org)
These findings highlight the potential of cucurbitacin I as a therapeutic agent that targets M2-like macrophages to inhibit cancer cell metastasis. (bvsalud.org)

Radioimmunoassay for detecting group-specific avian RNA tumor virus antigens and antibodies. (microbiologyresearch.org)
Innate and adaptive immune system: Cells and molecules involved in innate and adaptive immunity, antigens, antigenicity and immunogenicity. (pathfinderacademy.in)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

Conclusion】 The PERP 1 gene had the function of promoting granulosa cell apoptosis,and regulating its expression was of great significance for follicular development. (chvm.net)
Normally, these cells are eliminated by apoptosis. (medscape.com)
Other types repair the damage but instead pro- mutagens produce DNA damage, of DNA damage are oxidized or frag- cess it into a mutation, or direct the and they might more appropriately mented bases and the intercalation cell to undergo apoptosis. (who.int)

For instance, circFGFR1 may interact directly with miR-381-3p and function as a miRNA sponge to upregulate expression of miR-381-3p target gene C-X-C motif chemokine receptor 4, which enhances NSCLC progression as well as resistance to anti-programmed cell death 1-based therapy [ 11 ]. (biomedcentral.com)

They are very powerful in identifying co-expression or co-localization of RNA within specific sub-populations of cells. (acdbio.com)
DNMT3A expression is downregulated in intestinal epithelial cells from IBD patients and upon tumor necrosis factor treatment in murine intestinal organoids. (nature.com)
We determined the expression of components of the ECS in the human fetal testis from 6 to 17 developmental weeks and assessed the direct effects of phytocannabinoids Δ9-trans-tetrahydrocannabinol (THC) and cannabidiol (CBD) on the testis morphology and cell functions ex vivo. (biomedcentral.com)
Here we provide genome-wide profiles of 5hmC, 5fC, and p300 in Tdg fl/fl and Tdg-/- mESCs as well as a 5fC control (Non-NaBH4) and polyA RNA-Seq expression data. (nih.gov)
We now define the mechanism whereby maintenance of Hnf6 expression postnatally leads to β-cell dysfunction. (diabetesjournals.org)
Sustained expression of Hnf6 also results in downregulation of the β-cell-specific transcription factor MafA and a decrease in total pancreatic insulin. (diabetesjournals.org)
These results suggest that downregulation of Hnf6 expression in β-cells during development is essential to achieve a mature, glucose-responsive β-cell. (diabetesjournals.org)
Moreover, expression levels of DEGs were verified in HNSCC cells and tissues using reverse transcription‑quantitative (RT‑q)PCR. (spandidos-publications.com)
Unexpectedly, up- or downregulation of CASC9 expression in HNSCC cell lines with low or high CASC9 expression, respectively, did not result in significant changes of cell viability, clonogenicity, migration or chemosensitivity. (biomedcentral.com)
When a microRNA is present in the cell, it binds to the target sites and downregulates GFP expression, while in cells that do not express the microRNA GFP, it is expressed. (lu.se)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

However, the growth and differentiation of cells are influenced by the environment in which they are located. (encyclopedia.pub)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)

Researchers at the Children's Hospital of Philadelphia (CHOP) have created a flexible and affordable approach for targeted sequencing of full-length RNA molecules, which might hasten the development of novel diagnostics and therapies. (azolifesciences.com)

Errors at the early steps (RNA maturation, ribosomal decoding, and possibly membrane insertion) result in ribosomal stalling, which serves as a signal for the recruitment of the ribosome-associated quality control pathway (RQC) to degrade the stalled nascent chains. (cmmc-uni-koeln.de)

Genetic deletion of Dnmt3a in intestinal epithelial cells ( Dnmt3a ΔIEC ) in mice confirms the phenotype of an altered epithelial ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in the colon in vivo. (nature.com)
Quantitative real-time PCR (qRT-PCR), Western blotting (WB), cell phenotype detection, and RNA-seq techniques were used to evidence the effectiveness of the combination candidate. (bvsalud.org)

Effects of Lipofectamine 2000/siRNA complexes on autophagy in hepatoma cells Mol Biotechnol. (usc.edu)

There are several techniques used in fluorochrome detection, including fluorescence microscopy, flow cytometry, and fluorescence-activated cell sorting. (dadangoray.com)
Flow cytometry uses fluorochrome-labeled cells to identify and sort them based on their size, granularity, and fluorescence intensity. (dadangoray.com)
Fluorescence-activated cell sorting (FACS) is a more advanced version of flow cytometry that can sort cells at high speeds based on their fluorescence intensity and other criteria. (dadangoray.com)
When bound to RNA, a fluorescent signal is produced for the detection of single RNA molecules as diffraction-limited spots using conventional fluorescence microscopy. (bioscience.co.uk)
NB: Due to variable auto-fluorescence in the green channel inherent to cells and tissue, it is not recommended to use the FAM (fluorescein) for some Stellaris RNA FISH applications. (bioscience.co.uk)

Improvements at this stage may be especially important, since lapses here may be beyond correction at subsequent stages, which include the generation and detection of target fragments by MS, as well as the analysis of MS data by computational systems. (genengnews.com)
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. (xenbase.org)

(12 ). However, the versatility of the system allows the use of any microRNA of choice, including neuron-specific microRNAs ( 14 ). (lu.se)

Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)

Goupil S, Maréchal L, El Hajj H, Tremblay M-È, Richard FJ, Leclerc P (2016) Identification and Localization of the Cyclic Nucleotide Phosphodiesterase 10A in Bovine Testis and Mature Spermatozoa. (plos.org)

In children diagnosed with IBD, genome-wide DNA methylation profiling of purified intestinal epithelial cells clearly distinguishes between healthy individuals and the IBD group 8 . (nature.com)
Genome-wide mapping of 5fC in mouse embryonic stem cells (mESCs) reveals that 5fC preferentially occurs at poised enhancers among other gene regulatory elements. (nih.gov)

Some retroperitoneal extragonadal germ cell tumors may represent metastases from a testicular cancer , with subsequent spontaneous necrosis of the primary tumor. (medscape.com)

This includes understanding the critical biological mechanisms that alter or influence cell fate decisions. (ashg.org)
Cells have liver function tests and liver ultrasound and 2 antioxidant mechanisms for ensuring a biopsy. (who.int)

The most significant difference to other tools is the subsequent RNA-seq QC analysis Favipiravir cost step that Qualimap performs after computation of read counts. (buyresearchchemicalss.net)
Therefore, ignoring the spatial location information cannot accurately determine the microenvironment of the cells, which is not conducive to the subsequent analysis of cell function. (encyclopedia.pub)

Intestinal epithelial cells (IECs) have evolved to build a complex physiological barrier that separates the intestinal lumen from the underlying mucosal tissue. (nature.com)
Several functional components of the epithelium contribute to this unique role, among them the secretion of a mucus layer and life-long regeneration of the different epithelial cell types from a stem cell compartment, allowing for quick repair of barrier defects 2 . (nature.com)
Our cases comprised 1 patient with metastatic lung adenocarcinoma involving the brain (case 1), 1 patient with meningioma (case 2), and 1 patient with a metastatic perivascular epithelial cell tumor involving the liver ( Table ). (cdc.gov)

Cutting-edge technologies or novel uses of traditional methods to facilitate the detection (molecular or cytogenetic) and diagnosis of genetic disorders. (ashg.org)

The goal of our laboratory is to develop, scale up, and broadly disseminate molecular technologies for mapping cells and functional circuits. (stanford.edu)

We used the RNAscope 2.5 LS Probe-Hs-PPIB-sense probe (Advanced Cell Diagnostics), which is specific for peptidylprolyl isomerase B, to demonstrate the presence of intact RNA in the tissue sections. (cdc.gov)

The Onecut homeodomain transcription factor hepatic nuclear factor 6 (Hnf6) is necessary for proper development of islet β-cells. (diabetesjournals.org)

Replication of hepatitis C virus RNA on autophagosomal membranes J Biol Chem. (usc.edu)
Hepatitis C virus translation preferentially depends on active RNA replication PLoS One. (usc.edu)

When the B7.6 blastomeres divide, they produce two distinct populations of daughter cells, two primordial germ cells (B8.12) and two endodermal strand cells (B8.11) [ 4 ]. (biomedcentral.com)
[ 3 ] have issued guidelines that differ on specific points but agree on the value of prostate cancer screening in selected populations, for patients who agree to screening after a discussion of the risks and benefits. (medscape.com)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)

In this present study, we found that circIFT80 was abnormally overexpression in colorectal cancer tissues and tumor cells. (hindawi.com)
However, the smaller the residual mass, the lower the chance that it harbors viable tumor cells. (medscape.com)

However, when using flow cytometry for cell counting all. (web.app)
Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)

Germ cell formation has been investigated in sessile forms of tunicates. (biomedcentral.com)
In free-swimming tunicates, knowledge about germ cell formation is lacking. (biomedcentral.com)
vas is a well-known germ cell marker. (biomedcentral.com)
Depending on the molecules and testis age, highly deleterious effects of phytocannabinoid exposure were observed on testis tissue after 14 days, including Sertoli and germ cell death. (biomedcentral.com)
The only known risk factor for extragonadal germ cell tumors is Klinefelter syndrome (47XXY), which is associated with mediastinal nonseminomatous germ cell tumors, which are characterized by their location on the midline from the pineal gland to the coccyx. (medscape.com)
[ 1 , 2 ] In extragonadal germ cell tumors, no evidence of a primary malignancy is present in either the testes or ovaries by radiologic imaging or physical examination. (medscape.com)
Controversy remains regarding the origin of extragonadal germ cell tumors. (medscape.com)
The classic theory suggests that germ cell tumors (GCTs) in these areas are derived from local transformation of primordial germ cells misplaced during embryogenesis. (medscape.com)
During this stage of germ cell development, aberrant chromatid exchange events associated with crossing over can occur. (medscape.com)

cell detects it by means of the DNA result in a mutation. (who.int)

Traditional single-cell sequencing technologies rely on tissue dissociation in the acquisition of single cells, which abandons the spatial positional information of different types of cells. (encyclopedia.pub)
Detection of estradiol-induced messenger RNA (pS2) in uninvolved breast tissue from mastectomies for breast cancer. (xenbase.org)
RT‑qPCR demonstrated that hsa_circ_0035431, FUT6 and CGNL1 were downregulated in HNSCC cells and tissue and hsa‑miR‑940 was upregulated. (spandidos-publications.com)

By combining the PLK1 inhibitor volasertib and the ACLY inhibitor bempedoic acid, it showed a higher synergistic inhibition of cell viability and higher synergy scores in seven cell lines, compared with those of other combination treatments. (bvsalud.org)

GFP) cannot specifically visualize cell surface subpopulations. (neb.com)
Cell biologists traditionally use fluorescent dyes to label and visualize cells and the molecules within them under a microscope. (scitechdaily.com)
We thus created the means to explore the entire depth of a cell, and to visualize the molecules within it at the nanometer scale," said Jungmann. (scitechdaily.com)
These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)

Although cutaneous disease is the primary clinical manifestation of infection, TSPyV has been identified in blood, urine, cerebrospinal fluid, tonsils, and respiratory specimens by various methods, including nucleic acid detection, immunohistochemistry, and electron microscopy ( 2 , 4 - 6 ). (cdc.gov)

Anatomy29

Organisms19

Diseases1

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment61

Psychiatry and Psychology2

Phenomena and Processes68

Disciplines and Occupations4

Information Science7

Health Care6

Proteins19

Situ hybridization16

Circular RNAs5

Gene expression10

Protein21

Quantification1

Genes6

CircRNAs1

MRNA6

Probes15

Sequences3

Molecules in cells1

LncRNAs3

Isolation1

Localize3

Patterns4

Nucleotides1

Cellular10

Endothelial cells1

Vitro4

MRNAs1

Quantify specific2

Transcripts7

Genomic4

Proliferation7

Squamous cell carc4

Proteomics1

Detect6

Amplification3

Assays3

Biology8

Metastasis2

Antigens3

Apoptosis3

MiRNA1

Expression11

Differentiation3

Therapies1

Ribosome1

Phenotype2

Complexes1

Fluorescence5

Fragments2

MicroRNAs1

Endogenous1

20161

Genome-wide2

Metastases1

Mechanisms2

Subsequent2

Epithelial3

Cytogenetic1

Molecular1

RNAscope1

Transcription factor1

Replication2

Populations4

Tumor cells2

Flow cytometry2

Germ cell9

Detects1

Tissue3

Viability1

Visualize4

Immunohistochemistry1