Loading...
  • detect
  • Two molecular assays, INNO-LiPA Rif.TB (Innogenetics, Zwijndrecht, Belgium) and MisMatch Detect II (Ambion, Austin, Tex.), were performed on primary specimens and cultures to predict rifampin resistance, and these methods were compared with the resistance ratio method. (biomedsearch.com)
  • The methodology is able to detect even a single-copy of a specific nucleic. (google.com)
  • The invention is also provided in kit form for use in the clinical/diagnostic laboratory such that a relatively unskilled person can accurately and reproducibly detect even a single-copy of a specific nucleic acid of interest. (google.com)
  • Despite the fact that the starting material is not preamplified, bDNA assays can detect less than 100 copies of HIV-RNA per mL of blood. (wikipedia.org)
  • Other chip-based methods such as comparative genomic hybridization can detect genomic gains or deletions leading to LOH. (wikipedia.org)
  • Following the reports of the first use of ELISA to detect plant viruses by Voller and Clarke (1976), Dr. Sutula introduced in 1981 the first commercial kits for the detection of common viruses and bacteria in potato. (wikipedia.org)
  • deoxyribonucleic acid
  • 7. The method according to claim 1, wherein the target nucleic acid is a deoxyribonucleic acid. (google.com)
  • DNA denaturation, also called DNA melting, is the process by which double-stranded deoxyribonucleic acid unwinds and separates into single-stranded strands through the breaking of hydrophobic stacking attractions between the bases. (wikipedia.org)
  • array assay
  • The methods, kits and compositions of this invention are therefore particularly well suited for the analysis, and particularly single point mutation analysis, in a particle assay, in an array assay, in a nuclease digestion/protection assay and/or in a line assay format. (google.com)
  • Furthermore
  • Furthermore, DNA origami uses DNA hybridization interactions to realize a final structure, whereas SNAs and other forms of three-dimensional nucleic acids (structures templated with a triangular prism, rod, octahedra, or rhombic dodecadhedra-shaped nanoparticles) utilize the nanoparticle core to arrange the linear nucleic acid components into functional forms. (wikipedia.org)
  • comprises
  • 12. The method according to claim 1, wherein the method further comprises a post-hybridization rinsing step consisting of three separate rinses, each having a duration of between 5 and 30 minutes. (google.com)
  • wherein said matrix comprises charged functional groups that are suitable for binding nucleic acid under electrostatic binding conditions. (google.com)
  • Chlamydia
  • Aliquots were spiked with the equivalent of one C. trachomatis elementary body and were tested by three commercial assays: AMPLICOR CT/NG, Chlamydia LCX, and Chlamydia TMA. (asm.org)
  • Molecular technology has changed the horizon, and for Chlamydia trachomatis , for instance, molecular detection already is the gold standard technology. (asmscience.org)
  • isolation
  • Careful longitudinal followup utilizing highly sensitive assays and unique approaches to viral isolation are needed. (hindawi.com)
  • specimens
  • Consecutive urine specimens from 101 pregnant women and 287 nonpregnant women submitted for urinalysis were processed for C. trachomatis detection. (asm.org)
  • In addition, all three assays were partially inhibited by additional urine specimens. (asm.org)
  • While the majority of processed specimens are amplifiable, some contain substances that inhibit NAA, thereby giving false-negative results, even if the specimen contains C. trachomatis nucleic acid. (asm.org)
  • For the 38 primary specimens the LiPA and mismatch assay correlated with culture and subsequent identification and susceptibility tests in 36 and 38 specimens (94.7 and 100%), respectively. (biomedsearch.com)
  • polymerase
  • In the absence of external negative factors, the processes of hybridization and melting may be repeated in succession indefinitely, which lays the ground for polymerase chain reaction. (wikipedia.org)
  • bDNA
  • The design of the branched DNA and the way it is hybridized to the nucleic acid to be investigated differs between different generations of the bDNA assay. (wikipedia.org)