• The two new patent applications expand the growing intellectual property portfolio for the company and the innovative features that make this an ideal immunoassay system for the detection of antibodies to pathogens including Zika and other flaviviruses. (prnewswire.com)
  • Sign up to receive updates Complete the form below and we will keep you updated with the latest information on the development of the assays to identify IgM and IgG antibodies to the coronavirus or SARS-CoV-2. (beckmancoulter.com)
  • However, Tween 20 is not recommended for the detection of anti-cytoplasmic antibodies. (nih.gov)
  • The cancer assays to be tested use DNA analysis and antibodies to specific proteins as well as functional assays for proteins to attempt to identify bladder tumor presence. (centerwatch.com)
  • Enzyme-Linked Immunosorbent Assay (ELISA) uses antibodies and color change to detect and quantify proteins or other small molecules. (genscript.com)
  • Neutralizing antibodies can be detected through various diagnostic assays. (unl.edu)
  • This study evaluated the diagnostic characteristics of two neutralizing antibody assays for porcine epidemic diarrhea virus neutralizing antibodies in serum of challenged gilts. (unl.edu)
  • Samples were evaluated for the presence of neutralizing antibodies via a fluorescent focus neutralization assay and a high-throughput neutralization assay. (unl.edu)
  • The focus fluorescent neutralization and high-throughput neutralization assays can be used to monitor the status of neutralizing antibodies within animals or a population of animals. (unl.edu)
  • The antibodies were also used in the detection of AFBi in spiked grain samples by ELISA and BIAcore. (dcu.ie)
  • Both the polyclonal and scFv antibodies were found to be suitable for the detection of AFBi, and the assays were reproducible. (dcu.ie)
  • This ELISA method is for the semi-quantitative detection of IgA antibodies to tissue transglutaminase (endomysium) in human serum. (cdc.gov)
  • Currently, there are three basic types of tests to determine if an individual has been infected with SARS-CoV-2: viral nucleic acid (RNA) detection, viral antigen detection, and detection of antibodies to the virus. (medscape.com)
  • Although the overall sensitivity and specificity of the Western blot for detection of antibodies to the various viral proteins are high, there has been substantial debate regarding the interpretive criteria. (cdc.gov)
  • Plasmodium aldolase is a malaria biomarker measured using enzyme linked immunosorbent assay (ELISA) techniques. (hindawi.com)
  • ELISA Detection Kits contain all necessary reagents and buffers for performing 96-well plate ELISA. (genscript.com)
  • GenScript cAMP ELISA Detection Kit is a competition enzyme-linked immunosorbent assay which can be used for quantitative detection of cAMP (Adenosine 3', 5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. (genscript.com)
  • GenScript Human IL-8 ELISA Detection Kit is a solid phase sandwich ELISA for quantitative measurement of human IL-8 in serum, plasma and cell culture supernatant. (genscript.com)
  • GenScript His Tag Protein Detection Kit is developed for the detection of His-tagged protein expression and its relative quantification using His-tagged protein standards of known molecular weight (M.W.). This kit is based on competitive ELISA, suitable for most systems, such as bacterial, yeast, mammalian cell lysates and cell culture supernatant. (genscript.com)
  • GenScript GST tag ELISA Detection Kit is designed to quantitate glutathione-S-transferase (GST) or GST-fusion protein in samples such as cell lysate using a proprietary sandwich ELISA procedure in just 60 minutes. (genscript.com)
  • Randox Toxicology has launched three new ELISA testing kits for the detection of sedative drugs Zopiclone, Zaleplon, and Zolpidem. (mlo-online.com)
  • Randox Toxicology has developed three ELISA kits for the detection of Z drugs in urine and blood specimens. (mlo-online.com)
  • The Zolpidem ELISA offers a Limit of Detection (LOD) of 0.4ng/mL in urine and 0.52ng/mL in blood. (mlo-online.com)
  • The Zopiclone and Zaleplon ELISA kits are also available for rapid detection of these drugs. (mlo-online.com)
  • Learn more about Randox's ELISA kits for the detection of designer drugs. (mlo-online.com)
  • Enzyme-linked immunosorbent assays (ELISA) have been around as one of the primary methods of analyte detection for more than four decades. (ddw-online.com)
  • Over the years many changes to the basic format have resulted in assay improvements, but some of the most recent look set to take the traditional ELISA to new levels. (ddw-online.com)
  • These include many vendors now addressing the automation of ELISA assays. (ddw-online.com)
  • Other automated instrumentation is designed to support the creation/coating of ELISA assay plates or contains cartridge-based delivery of assay reagents to reduce set-up time. (ddw-online.com)
  • These are supported by a wide range of ELISA reagents, assay and kits providing relative profiling or absolute quantitation of proteins across a wide range of study areas. (ddw-online.com)
  • When coupled with the breadth of existing global expertise in immunoassays, it is easy to see why ELISA still remains the methodology of choice by those labs tasked with analyte detection. (ddw-online.com)
  • E nzyme-linked immunosorbent assays (ELISA) have been one of the primary methods of detecting the presence of a substance, usually an antigen, in an aqueous sample for more than 40 years. (ddw-online.com)
  • However, numerous variants of this format have been developed over the years (eg indirect, sandwich, competitive, etc) and a multitude of detection chemistries have been applied to ELISA, including chemiluminescence and various fluorescent readouts. (ddw-online.com)
  • ELISA has gained widespread acceptance across a very diverse range of fields (eg diagnosis of infectious diseases, food allergen detection, plant pathogens to biomarkers), but it never achieved significant adoption by early drug discovery labs. (ddw-online.com)
  • The immunoassay landscape has, however, dramatically changed in recent years with the development of reduced assay steps protocols, no wash ELISAs and new automated systems designed to run ELISA. (ddw-online.com)
  • More recently, bead-based methods and microfluidic platforms have started to impact immunoassays and by coupling with single molecule detection look set to take the sensitivity of traditional ELISA to new levels. (ddw-online.com)
  • This review article uses as its starting point some of the findings of HTStec's ELISA Assay Trends 2012 survey and report, published in June 2012 (1). (ddw-online.com)
  • This report discusses end-users' current practices and preferences in ELISA assays, and seeks to understand future user requirements. (ddw-online.com)
  • The main (primary) application area for survey respondents' ELISA assays was immunology (17% selecting). (ddw-online.com)
  • Dot-enzyme-linked immunosorbent assay (Dot-ELISA) for detection of pneumococcal polysaccharide antigens in pleural fluid effusion samples. (scielo.br)
  • Comparison with bacterial culture, counterimmunoelectrophoresis and latex agglutination Dot-enzyme-linked immunosorbent assay (Dot-ELISA) for detection of pneumococcal polysaccharide antigens in pleural fluid effusion samples. (scielo.br)
  • A dot-enzyme-linked immunosorbent assay (Dot-ELISA) for pneumococcal antigen detection was standardized in view of the need for a rapid and accurate immunodiagnosis of acute pneumococcal pneumonia. (scielo.br)
  • Pneumococcal omniserum proved to be an optimal polyvalent antiserum for the detection of pneumococcal antigen by Dot-ELISA. (scielo.br)
  • The laboratory method is an Enzyme-Linked Immunosorbant Assay (ELISA). (cdc.gov)
  • Different methods have been developed for the detection of tTGA of which enzyme-linked immunosorbent assays (ELISA), radiobinding assays (RBA) and electrochemiluminescence (ECL) assays are the most commonly used. (lu.se)
  • 1999), and enzyme-linked immunosorbent assay (ELISA) methods (Trochimowicz et al. (cdc.gov)
  • Three different assays are approved by the US Food and Drug Administration (FDA) for clinical use, Abbott HCV enzyme immunoassay (EIA) 2.0 (Abbott laboratories), ORTHO HCV version 3.0 enzyme-linked immunoassay (ELISA) (Ortho-Clinical Diagnostics) and VITROS Anti-HCV assay (Ortho-Clinical Diagnostics). (medscape.com)
  • Qualitative tests only detect the presence of HCV RNA (specificity, approximately 98%) whereas the quantitative assays detect the viral load in IU/mL (specificity, approximately 98-99%) and provide adequate information that guides clinicians to manage and monitor treatment outcome. (medscape.com)
  • 1 With the high degree of cross-reactivity within the flaviviridae family, a diagnostic assay that can rapidly detect and differentiate between them, and determine new vs old infection, is of upmost importance and is a current area of focus for bioLytical. (prnewswire.com)
  • We developed two quantitative PCR assays for use with environmental DNA (eDNA) to detect numerous species in the genus Cottus that are indigenous to the Pacific coast watersheds of North America. (usgs.gov)
  • Objective -To develop a reverse transcriptase-polymerase chain reaction (RT-PCR) assay to detect canine melanoma-associated antigens (MAAs) and to use this technique to screen aspirates of lymph nodes (LNs) for evidence of metastatic spread of oral malignant melanoma. (avma.org)
  • Using IHHNV plasmid standards and DNA samples from a variety of organisms, we evaluated the ability of the IHHNV-RPA assay to detect IHHNV based on analysis of its sensitivity, specificity, rapidity, and reproducibility. (stir.ac.uk)
  • Probit analysis of eight independent experimental replicates indicated satisfactory performance of the RPA assay, which is sufficiently sensitive to detect as few as 4 copies of the IHHNV genome within 7min at 39°C with 95% reliability. (stir.ac.uk)
  • The purpose of this study was to develop a real time polymerase chain reaction ( PCR ) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories .Sanger sequencing and a newly developed locked nucleic- acid , real-time PCR assay were used to detect the JAK2V617F mutation . (bvsalud.org)
  • Overall, these assays and approaches detect specific mutations or features such as insertions, deletions and point mutations in the SARS CoV-2 genome that are characteristic of a particular VOC/VOI using PCR. (who.int)
  • Molecular assays: These detect viral nucleic acid and can be qualitative or quantitative. (medscape.com)
  • Pick up reagents and pre-defined assays for COVID-19, other viruses, and microbes. (biosearchtech.com)
  • Wooden shafts or calcium alginate swabs may contain substances that inhibit some viruses and nucleic acid detection reactions. (medscape.com)
  • Conclusion: Constant monitoring of genetic changes in the circulating influenza A(H1N1)pdm09 viruses is important for maintaining the sensitivity of molecular detection assays. (who.int)
  • A number of tools are available for the detection of mutations that are characteristic of viruses defined as VOI/VOCs. (who.int)
  • Reveal for Histamine (PN 9548) is a qualitative lateral flow assay intended for visual screening of histamine at 50 parts-per-million (ppm) in scombroid species of fish, such as tuna and mahi-mahi. (neogen.com)
  • The World Health Organization (WHO) recommends nucleic acid testing for qualitative or quantitative HCV RNA detection as well as for test of cure at 12 or 24 weeks following antiviral treatment completion. (medscape.com)
  • Khan NA, Jarroll EL, Paget TA (2001) Acanthamoeba can be differentiated by the polymerase chain reaction and simple plating assays. (springer.com)
  • Here, we describe a novel real-time isothermal recombinase polymerase amplification (RPA) assay developed for IHHNV detection. (stir.ac.uk)
  • Xia X, Yu Y, Hu L, Weidmann M, Pan Y, Yan S & Wang Y (2015) Rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) by real-time, isothermal recombinase polymerase amplification assay. (stir.ac.uk)
  • In addition to precise target quantification, a 5-plex dPCR run also shows highly specific detection of different microbial water pathogens (see figure Water microbial pathogen detection in 5-plex on QIAcuity ). (qiagen.com)
  • FORT COLLINS, Colo. , Sept. 19, 2023 /PRNewswire/ -- GT Molecular, Inc., a company providing ultrasensitive PCR assays for wastewater-based epidemiology testing, pathogen detection, and cancer research is pleased to announce an agreement with Roche to provide a highly multiplexed respiratory panel for wastewater surveillance on the new Roche Digital LightCycler® System. (wreg.com)
  • It is designed around modern-day food processors' needs for a real-time pathogen detection approach that's faster and simpler while also more accurate. (food-safety.com)
  • We propose to assess the use of a multiple mutation-based assay, using DNA from exfoliated cells in the urine of patients, to establish the sensitivity and specificity in tumor detection compared to cystoscopy and cytology. (centerwatch.com)
  • Diagnostic sensitivity and specificity for the fluorescent focus neutralization and high-throughput neutralization assays for this study were optimized at a cutoff of a dilution of 80 and 80% fluorescent reduction respectively and demonstrated moderate agreement based off the kappa statistic. (unl.edu)
  • These tests have very high sensitivity and specificity for HCV detection (sensitivity of greater than 99%, specificity of 99% in immunocompetent patients). (medscape.com)
  • The portfolio consists of over 680 different assays, 200 of which are dPCR wet-lab tested. (qiagen.com)
  • In addition, we propose to isolate free-DNA for use in molecular assays. (centerwatch.com)
  • Methods Multiplex real-time PCR method (Xpert CT/NG, Cepheid ) has been tested versus N. gonorrhoeae standard culture and C. trachomatis molecular assay ( Artus C. trachomatis TM PCR Kit, Qiagen). (bmj.com)
  • More advanced diagnostic tests such as enzyme linked immunosorbent assays (ELISAs) do possess this capability but have similar drawbacks to microscopy, requiring expensive equipment, long incubation times, and trained staff. (hindawi.com)
  • In order to find new modulators for this important enzyme, an innovative cell-based assay has been developed and optimized for the use in high-throughput screening. (nih.gov)
  • Here, we report the clinical validation of the specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) assay using the enzyme Cas13a from Leptotrichia wadei for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-the virus that causes coronavirus disease 2019 (COVID-19)-in 154 nasopharyngeal and throat swab samples collected at Siriraj Hospital, Thailand. (nature.com)
  • Primers for RT-LAMP or RT-RPA can be designed for specific viral RNA sequences, and the amplification methods are used before the Cas enzyme detection step, to multiply detection signals from the specific nucleic acid sequences. (nature.com)
  • There is also an assortment of bead coatings available that allows this method to be applied to a broad range of applications, such as enzyme assays and radio-immuno assays. (wikipedia.org)
  • We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. (lu.se)
  • The aim of this study in Egypt was to estimate the usefulness of an interferon-gamma release assay (IFN-γ) assay for the detection of latent TB infection in contacts of active TB cases. (who.int)
  • The two new patent applications are based on the Company's INSTI platform and describe methods and kits allowing either simultaneous or sequential detection of different antibody classes to multiple pathogens using a single sample of blood or blood product. (prnewswire.com)
  • This digital PCR assay kit co-quantifies respiratory pathogens SARS-CoV-2, influenza A, influenza B, and RSV, as well as an internal process control all within a single reaction well. (wreg.com)
  • We found that the use of Tween 20 in the IB procedure significantly improved the binding of autoantibodies to Jo-1, Scl70, (U1)RNP 68 kDa and C, Sm B/B' and D. Moreover, it increased the sensitivity for the detection of anti-Sm D peptide in systemic lupus erythematosus (SLE) sera with no decrease in specificity. (nih.gov)
  • The high-throughput assay has advantages over the focus fluorescent assay in that it has a higher specificity at the indicated cut-off and the nature of the results allows for more discrimination between individual results. (unl.edu)
  • Sequencing of canine Melan- A and gp100 PCR products confirmed the specificity of the assay for these genes. (avma.org)
  • The current study reports on the analytical performance of the Abbott RealTime CT/NG assay, including the limit of detection, inclusivity, and analytical specificity for C. trachomatis and N. gonorrhoeae in rectal and pharyngeal specimens. (escholarship.org)
  • GT-Digital Wastewater Surveillance Respiratory Pathogen Assay Kit for the Roche Digital LightCycler® System detects and quantifies influenza A/B, SARS-CoV-2, Respiratory Syncytial Virus (RSV) A/B, including an internal process control and a fecal indicator. (wreg.com)
  • Using the Roche Digital LightCycler®, GT Molecular developed a multiplex assay kit targeting influenza A, influenza B, RSV A/B, SARS-CoV-2, along with all necessary controls. (wreg.com)
  • This study investigated how these missed detections occurred to determine their effect on the surveillance of influenza. (who.int)
  • They compared the sensitivity of the new assay technique, the timed prion seeding assay (tASA), to other currently available tests (two bioassays in laboratory rodents and three commercially available TSE rapid tests). (medicalnewstoday.com)
  • We have a BRAF assay [for] a melanoma mutation, a resistance mutation" similar to Roche's US Food and Drug Administration-approved Cobas 4800 BRAF V600 companion diagnostic for BRAF mutation-positive metastatic melanoma. (genomeweb.com)
  • Comparison of Two Diagnostic Assays for the Detection of Serum Neutra" by Justin Brown, Korakrit Poonsuk et al. (unl.edu)
  • Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
  • The dPCR Microbial DNA Detection Assays and the QIAcuity dPCR System enable accurate digital PCR-based quantification. (qiagen.com)
  • A comparison between singleplex and multiplex analysis using the dPCR Microbial DNA Detection Assays shows similar and highly precise quantification for all targets (see figure Microbial detection in multiplex dPCR on QIAcuity ). (qiagen.com)
  • L'objectif de la présente étude conduite en Égypte était d'évaluer l'utilité d'un test de quantification de la libération d'interféron gamma (IFN‑γ) pour le dépistage des infections tuberculeuses latentes chez les contacts de patients atteints de tuberculose active. (who.int)
  • Subjects diagnosed as latent TB infection by IFN-γ assay (QuantiFERON®-TB Gold In-Tube) were 5.9% of controls, 31.0% of casual contacts and 33.3% of close contacts. (who.int)
  • The IFN-γ method could be more helpful than TST for detection of latent TB infection in contacts. (who.int)
  • These assays are 97% specific but cannot distinguish acute from chronic infection. (medscape.com)
  • The recombinant immunoblot assay is used to confirm HCV infection. (medscape.com)
  • Viral tests (nucleic acid or antigen detection tests) are used to assess acute infection, whereas antibody tests provide evidence of prior infection with SARS-CoV-2. (medscape.com)
  • Pneumococcal omniserum diluted at 1:200 was employed in this assay for antigen detection. (scielo.br)
  • Then, further protocol development and standardization to achieve a clinical sensitivity for CIN2/3 detection close to what can be achieved for invasive lesions using the physician collected cervical is needed. (plos.org)
  • 1995). At the time of publication there was limited knowledge of molecular changes underlying the different clinical pathways outlined above and our assay was based on one gene (c-H-ras-1). (centerwatch.com)
  • These findings suggest that nanoPCR assay has tremendous potential in the clinical diagnosis of parasitic infections as well as for studying epidemiology and pathology and environmental monitoring of other microbes. (springer.com)
  • 45 clinical samples (female and male urogenital swabs, ocular infections, first void of urine, seminal fluid, and external quality control samples - UK NEQAS) were selected and stored at - 20°C. Xpert CT/NG allowed to perform in one-step extraction, amplification and detection of C.trachomatis and N. gonorrhoeae DNA directly from all the variety of selected clinical specimens, within 60 min. (bmj.com)
  • Both types of assays in association with the clinical presentation (jaundice, ALT elevation) have been used to adequate identify acute or chronic HCV infections. (medscape.com)
  • Each assay is based on an endpoint PCR amplification of a species-specific genetic region of the relevant microbe or a region of an individual microbial gene. (qiagen.com)
  • Nucleic acid detection by isothermal amplification and the collateral cleavage of reporter molecules by CRISPR-associated enzymes is a promising alternative to quantitative PCR. (nature.com)
  • RT-RPA and RT-LAMP are highly sensitive methods, but can suffer from nonspecific amplification under isothermal conditions, leading to false-positive results when used for viral RNA detections. (nature.com)
  • Detection of loop-mediated isothermal amplification (LAMP) end product by color changes. (ajtmh.org)
  • The 3M™ Molecular Detection System, first introduced to food processors, third-party reference laboratories and other customers in 2011, is based on unique isothermal DNA amplification and bioluminescence detection technologies. (food-safety.com)
  • It is a conversion assay that uses recombinant prion-related protein as a substrate and detects conversion via changes in fluorescence. (medicalnewstoday.com)
  • Within a detection limit of 42 RNA copies per reaction, SHERLOCK was 100% specific and 100% sensitive with a fluorescence readout, and 100% specific and 97% sensitive with a lateral-flow readout. (nature.com)
  • Homogeneous Proximity Tyrosine Kinase Assays: Scintillation Proximity Assay versus Homogeneous Time-Resolved Fluorescence. (wikipedia.org)
  • The study aims to compare the detection rates of high-risk human papillomavirus 16 and 18 E6 oncoprotein in urine, vaginal self-collected, and cervical scrapes of women using the Onco E6 ™ Cervical Test and compare the HPV16 and/or HPV18 E6 detection rates with the HPV DNA testing. (plos.org)
  • CMO, LWM, JSS, JS, JHTGF and ALF are listed as coinventor of a "urine-based cancer detection test and methods of using the same" provisional patent no 62/525,959. (plos.org)
  • The purpose of this study is to determine if analysis of DNA and protein material found in urine will be useful in the detection of urothelial cancer of the bladder and kidney. (centerwatch.com)
  • The remaining urine will be stored to evaluate biomarkers for the detection of tumor presence or progression using protein-based analyses. (centerwatch.com)
  • The primary objective of this study is to evaluate the utility of emerging technologies in the detection of bladder tumor cells using non-invasive approaches utilizing voided urine samples. (centerwatch.com)
  • Reveal and Reveal Q+ assays are easy-to-use lateral flow tests that utilize a simple water extraction method, providing results in a matter of minutes and eliminating the need to dispose of any chemicals, as is needed with other commercially available histamine detection methods, reducing testing time. (neogen.com)
  • The method also improves mutation detection because "with our XNA clamp, if you get a single nucleotide polymorphism, the mismatch causes a 15 to 20 degree difference in [melt] temperature" - a 10-fold increase over other PCR methods, he said. (genomeweb.com)
  • However, DiaCarta is also currently developing a biotin-based detection method that could obviate the need for a real-time PCR system, instead relying on simpler detection methods. (genomeweb.com)
  • The assays provide rapid results after as little as 24 hours of enrichment--days faster than the standard methods. (food-safety.com)
  • The AOAC Official Methods of Analysis carry a reputation for the highest standards of excellence and rigorous analysis, and we are proud that our assays are recognized by this globally-recognized agency," said John David, global marketing supervisor with 3M Food Safety. (food-safety.com)
  • For its 3M Molecular Detection System, 3M also has AOAC International First Action Official Methods of Analysis status for its 3M Molecular Detection Assay Salmonella and both AOAC PTM and NF Validation by AFNOR Certification for its 3M Molecular Detection Assay Salmonella and 3M Molecular Detection Assay E.coli O157 (including H7), in addition to certifications from government and regulatory organizations in other countries around the world. (food-safety.com)
  • In comparison to the previous over-coated plate-based methods, SPA has a number of advantages that makes it more popular: Assay flexibility - the concentration of the components in the assay can be adjusted to suit the user due to the higher surface area design of the SPA beads, hence providing the user flexibility in designing assay. (wikipedia.org)
  • Additionally, analytical methods are included that modify previously used methods to obtain lower detection limits and/or to improve accuracy and precision. (cdc.gov)
  • Standard EPA methods include Infrared spectroscopy, GC separation with flame ionization detection, and HPLC with detection at 254 nm (ultraviolet [UV]) (Stafford et al. (cdc.gov)
  • The objective of this document is to outline the available methods and tools for screening and sequencing patient specimens for the detection of VOC or VOI, with a focus on those that offer a rapid turn-around time so as to support rapid implementation of public health actions for COVID 19 control. (who.int)
  • These analytical performance data demonstrate that the Abbott CT/NG RealTime assay is an accurate, sensitive, and specific assay in rectal and pharyngeal specimens, supporting the potential of the assay for detection of rectal and pharyngeal C. trachomatis and N. gonorrhoeae infections. (escholarship.org)
  • The RTPCR assay was performed by use of tyrosinase, Melan-A, gp100, tyrosinase-related protein 2 (TRP-2), or melanoma antigen-encoding gene B (MAGE-B)- specific primers. (avma.org)
  • Multiplex dPCR-based detection of microbial targets offers a flexible setup of small panels in fewer reactions, thus conserving precious samples. (qiagen.com)
  • DiaCarta has other commercial assays, a line of human papillomavirus tests that use branched DNA technology licensed from Siemens. (genomeweb.com)
  • Tissue transglutaminase assay (IgA-TTG) and the IgA endomysial antibody assay (IgA EMA) estimates the prevalence of celiac disease in the United States population. (cdc.gov)
  • You can combine your own assays for multiplexing by ordering single assays with different dyes (FAM, HEX, TAMRA, ROX or Cy5). (qiagen.com)
  • In addition to the XNA clamp, DiaCarta claims it has improved detection by using colorimetric dyes instead of hydrolysis-based probes to reveal mutant amplicons. (genomeweb.com)
  • The problem is exacerbated if non-sequence-specific probes, such as pH-sensitive dyes, are used for the detection 6 . (nature.com)
  • Initial direct viral detection is typically performed using an upper respiratory tract (URT) specimen. (medscape.com)
  • LANSING, Mich., May 31, 2023 - Neogen® Corporation (NASDAQ: NEOG) announced today that it has launched two new assays for the detection of histamine, Reveal® for Histamine and Reveal Q+ for Histamine. (neogen.com)
  • Powell added that the company is already able to perform V600E BRAF mutation detection on such a strip, although this version of the assay is not yet commercially available. (genomeweb.com)
  • Few assays approved by the US Food and Drug Administration are commercially available to diagnose pharyngeal or rectal infections. (escholarship.org)
  • We utilize datasets from multiple cooperating laboratories to demonstrate both a discrete threshold approach and a curve‐fitting modeling approach for determining LODs and LOQs for eDNA qPCR assays. (usgs.gov)
  • Ultimately, standardization of how LOD and LOQ are determined, interpreted, and reported for eDNA assays will allow for more informed interpretation of assay results, more meaningful interlaboratory comparisons of experiments, and enhanced capacity for assessing the relative technical quality and performance of different eDNA qPCR assays. (usgs.gov)
  • Significant advances in assay speed and throughput are demonstrated, as well as important improvements in sensitivity. (ucl.ac.uk)
  • RICHMOND, BC , Aug. 19, 2016 /PRNewswire/ - bioLytical Laboratories, a world leader in rapid infectious disease tests, is pleased to announce that it has filed two patent applications with the U.S. Patent and Trademark Office relating to multiple color rapid detection assay systems. (prnewswire.com)
  • These technology breakthroughs can revolutionize point-of-care rapid assays for detection of acute infections including Zika. (prnewswire.com)
  • Here, we developed a nanoPCR assay for the rapid detection of brain-eating amoebae using various nanoparticles. (springer.com)
  • Scintillation proximity assay (SPA) is an assay development and biochemical screening that permits the rapid and sensitive measurement of a broad range of biological processes in a homogeneous system. (wikipedia.org)
  • Reveal Q+ for Histamine (PN 9549) is a quantitative lateral flow assay designed to be read on Neogen's Raptor® Integrated Analysis Platform. (neogen.com)
  • The assay, which we show is amenable to multiplexed detection in a single lateral-flow strip incorporating an internal control for ribonuclease contamination, should facilitate SARS-CoV-2 detection in settings with limited resources. (nature.com)
  • RNAscope technology is an advanced in situ hybridization assay that enables visualization of single-molecule gene expression with single-cell resolution directly in intact cells and tissues. (genomeweb.com)
  • We conclude that the addition of Tween 20 to standard IB is advantageous for anti-nuclear antigen antibody detection and improves the sensitivity of the method in revealing anti-Sm-positive sera in SLE. (nih.gov)
  • P erhaps most significantly are the big increases in assay sensitivity and the widening of the dynamic range accomplished by using alternatives to the traditional colorimetric detection, or by the use of new platform technologies. (ddw-online.com)
  • For bacteria, the assays target the 16S rRNA gene, and for fungi, they target ribosomal RNA genes. (qiagen.com)
  • Bio-Techne has expanded its Advanced Cell Diagnostics-branded RNAscope technology with the release of RNA-Protein Co-Detection Assays. (genomeweb.com)
  • Timed amyloid seeding assay achieves or surpasses sensitivity of currently available tests for transmissible spongiform encephalopathies, according to a report in The Journal of Molecular Diagnostics . (medicalnewstoday.com)
  • A study in The Journal of Molecular Diagnostics describes an advanced assay that offers better sensitivity than currently available tests for detecting a prion disease affecting elk. (medicalnewstoday.com)
  • However, product information on Roche's website claims its BRAF test detects mutations at less than a 5 percent mutation level, while DiaCarta claims its XNA method enables detection of less than 0.1 percent mutated DNA. (genomeweb.com)
  • The assay detects single and double-stranded DNA breaks by measuring the migration of DNA from individual nuclei following alkaline treatment. (neb.com)
  • Our dPCR Microbial DNA Detection Assays can be combined for multiplex analysis. (qiagen.com)
  • N2 - The article reviews the development of TRAP assay and its modifications. (muni.cz)
  • dPCR Microbial DNA Detection Assays are intended for molecular biology applications. (qiagen.com)
  • For the dPCR reaction, the assay is combined with the QIAcuity Probe PCR Kit (DNA targets) or the QIAcuity OneStep Advanced Probe Kit (RNA targets). (qiagen.com)
  • Or, you can choose from our offering of 5-plex assay bundles, which have already wet-lab tested in dPCR by our scientists. (qiagen.com)
  • The dPCR Microbial DNA Detection Assays are intended for use in nanoplate digital PCR. (qiagen.com)
  • This luminescent assay, which is suitable for both 96- and 384-well plate formats, has been achieved by stably expressing the alpha and beta subunits of a mutated form of sGC in Chinese hamster ovary cells. (nih.gov)
  • We demonstrated that this cell line responds to compounds typically used in the field of sGC research and that it represents an innovative and robust assay to screen for sGC modulators with high efficiency and high sensitivity by means of standard luminescence readers. (nih.gov)
  • The codetection assays allow researchers to simultaneously examine gene expression specific to cell types and identify cellular sources of secreted proteins. (genomeweb.com)
  • The mutated form synthesizes cyclic adenosine 3',5'-monophosphate instead of cGMP, allowing the detection of enzymatic activity by a reporter gene approach. (nih.gov)
  • Currently, manufacturers are focusing on and targeting assays to mutations in the S gene. (who.int)
  • Addressing the need to define steadfast analytical performance criteria for prion amyloid seeding assays (ASAs), researchers developed a method to measure prion protein conversion time (from normal cellular form to prion form) by a combination of statistical analyses to obtain a prion-detecting ASA with a known degree of confidence. (medicalnewstoday.com)
  • Creative Proteomics is an innovative contract research organization that focuses on using advanced equipment platforms and optimized detection technologies to help researchers around the world reduce time and costs. (thomasnet.com)
  • Although these RDTs are cheap, fast, and easy-to-use, they do not provide a quantitative estimate of parasitic burden and are less sensitive for detection of Plasmodium vivax [ 1 , 2 ]. (hindawi.com)
  • The third study will develop and optimize an ASSURED paper-based assay to determine iron content in fortified foods that interfaces with a smartphone application. (illinois.edu)
  • This method also allows the user to optimize the sensitivity of the assay by altering the quantity of SPA beads. (wikipedia.org)