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PolyomavirusPolyomavirus InfectionsAntigens, Polyomavirus TransformingMerkel cell polyomavirusAntigensBK VirusAntigens, Viral, TumorJC VirusCarcinoma, Merkel CellAntigens, BacterialTumor Virus InfectionsAntigens, NeoplasmAntigens, SurfaceAntigens, ViralDNA, ViralMerkel CellsCapsid ProteinsMolecular Sequence DataSensitivity and SpecificitySimian virus 40Antigens, ProtozoanPolymerase Chain ReactionBase SequenceCell LineLeukoencephalopathy, Progressive MultifocalHLA AntigensAntigens, FungalAntigens, HelminthAntigens, CDH-2 AntigensCell Transformation, ViralAntibodies, MonoclonalLimit of DetectionPolyomaviridaeEnzyme-Linked Immunosorbent AssayCarcinoembryonic AntigenVirus ReplicationDNA ReplicationAmino Acid SequenceCross ReactionsAntibodies, ViralCapsidHLA-DR AntigensEpitopesReceptors, Antigen, T-CellUrineMutationHistocompatibility AntigensProliferating Cell Nuclear AntigenImmunoglobulin GProstate-Specific AntigenGenes, ViralCells, CulturedHistocompatibility Antigens Class IIT-LymphocytesO AntigensMice, Inbred BALB CReceptors, Antigen, B-CellFluorescent Antibody TechniqueEnhancer Elements, GeneticAntigens, Tumor-Associated, CarbohydrateAntigens, CD15Oncogene Proteins, ViralViral ProteinsHLA-A2 AntigenGenome, ViralProto-Oncogene Proteins pp60(c-src)Antigens, CD8Plasmids3T3 CellsTransfectionAntibody SpecificityHepatitis B Surface AntigensBlood Group AntigensImmunoenzyme TechniquesAntigens, CD3DNA PrimersViral Regulatory and Accessory ProteinsTime FactorsAntibodies, BacterialSequence Analysis, DNAHLA-A AntigensImmunoassayTranscription, GeneticHistocompatibility Antigens Class ICloning, MolecularReproducibility of ResultsKidney TransplantationLymphocyte ActivationRecombinant ProteinsVirologyMolecular WeightHepatitis B AntigensImmune SeraGene Expression Regulation, ViralAntigen-Antibody ReactionsHLA-D AntigensReceptors, AntigenAntigens, CD45RNA, Viral
Large T-antigMiddle T antigenTumorEpithelialSarcoma virusImmunohistochemicalGenetically engineeredTransgenic miceSubsetStainCellularIdentificationGrowth factor-betaPrimaryCellsHumanStudiesDevelopmentEarlyMerkelProgressive Multifocal LeukoencPapillomavirus infectionNeoplastic cellsTumorsLocalizationSV40ProteinProteinsOncogenesisSequencesGene productIsolationExpressionAntibodiesPositiveTissuePhiladelphiaVirusEarlySpecificVariety
Large T-antig3
  • The multifunctional nuclear protein large T-antigen was detectable by immunohistochemical analyses in a subset of neoplastic cells. (cdc.gov)
  • Furthermore, transgenic mice harboring the viral-encoded large T-antigen (LT-Ag) alone develop tumors of neuroectodermal origin, including malignant peripheral nerve sheath tumors (MPNSTs) and glioblastomas. (cdc.gov)
  • CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). (lookformedical.com)
Middle T antigen1
  • In this study, using polyoma middle T antigen (PyMT) mice, a widely used luminal B breast cancer model, we profiled microRNA (miRNA) expression at four time points that represent different key developmental stages of cancer progression. (biomedcentral.com)
Tumor5
  • We have identified a candidate etiologic agent, dubbed raccoon polyomavirus, that was present in the tumor tissue of all affected animals but not in tissues from 20 unaffected animals. (cdc.gov)
  • However, natural disease studies of human infection and experimental disease studies suggest that a potential outcome of some polyomavirus (PyV) infections is tumor formation ( 4 - 6 ). (cdc.gov)
  • Studies reliant on molecular detection of tumor-associated virus in isolation, however extensive, are inconclusive because association between PyVs and naturally occurring neoplasms varies and because PyV infections are highly prevalent, yet tumor formation is rare ( 3 , 4 , 12 - 14 ). (cdc.gov)
  • [ 7 ] In KAs, cells that stain positive with proliferating-cell nuclear antigen immunostaining are distributed only in the outer edges of the tumor, corresponding to the proliferating squamous epithelial cells. (medscape.com)
  • A highly immunogenic tumor transfected with a murine transforming growth factor type beta 1 cDNA escapes immune surveillance. (academicinfluence.com)
Epithelial3
  • Clear cell acanthoma usually stains positively for epithelial membrane antigen and negatively for carcinoembryonic antigen. (medscape.com)
  • Transforming growth factor-beta1 mediates epithelial to mesenchymal transdifferentiation through a RhoA-dependent mechanism. (academicinfluence.com)
  • Epithelial membrane antigen-reactive feline chordoid meningioma in a European wildcat (Felis silvestris). (unibe.ch)
Sarcoma virus2
  • Identification of a transformation-specific antigen induced by an avian sarcoma virus. (wikipedia.org)
  • The specific interaction of the Rous sarcoma virus transforming protein, pp60src, with two cellular proteins. (wikipedia.org)
Immunohistochemical1
  • Immunohistochemical detection of SV40 T antigen has been used to characterize the hepatic cell populations expressing the transgene. (ox.ac.uk)
Genetically engineered1
  • adoptive transfer of genetically engineered t-cells to express antigen-specific t-cell receptor (tcr) is a feasible and effective therapeutic approach for numerous types of cancers, including epstein-barr virus (ebv)-associated malignancies. (liverpool.ac.uk)
Transgenic mice1
  • We have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. (ox.ac.uk)
Subset1
  • In mice stimulated by anaemia or hypobaric hypoxia, SV40 T antigen was demonstrated in two liver cell populations: a subset of hepatocytes and a nonparenchymal cell type. (ox.ac.uk)
Stain1
  • In contrast, cells within an SCC that stain positive with proliferating-cell nuclear antigen immunostaining are more diffusely distributed. (medscape.com)
Cellular1
Identification1
  • Genome sequencing is rapidly gaining traction as a diagnostic and surveillance tool for clinical settings, where the profiling of co-infections, identification of imported malaria parasites, and detection of drug resistance are crucial for infection control and disease elimination. (bvsalud.org)
Growth factor-beta1
  • Induction of c-sis mRNA and activity similar to platelet-derived growth factor by transforming growth factor beta: a proposed model for indirect mitogenesis involving autocrine activity. (academicinfluence.com)
Primary1
  • Early detection and treatment of primary tumours is crucial to limit progression and local invasion of deep tissues. (unav.edu)
Cells4
  • Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells. (ox.ac.uk)
  • SV40 is a powerful carcinogen that, in vitro , transforms human mesothelial cells. (sv40.org)
  • Growth inhibitor from BSC-1 cells closely related to platelet type beta transforming growth factor. (academicinfluence.com)
  • Transforming growth factor production by chemically transformed cells. (academicinfluence.com)
Human1
  • Reversible inhibition of normal human prokeratinocyte proliferation by type beta transforming growth factor-growth inhibitor in serum-free medium. (academicinfluence.com)
Studies2
  • Recent advances have been made by an accumulation of studies on Merkel cell polyomavirus (MCPyV), which is highly associated and integrated in most Merkel cell carcinomas ( 5 ). (cdc.gov)
  • In order to distinguish between KA and SCC, further histologic studies can be used, including proliferating-cell nuclear antigen immunostaining. (medscape.com)
Development1
  • Raccoon polyomavirus may contribute to the development of malignant brain tumors of raccoons. (cdc.gov)
Early1
  • Thus, continuous extension of our knowledge of the repertoire of viruses prone to infect the central nervous system (CNS) is vital for pathogen monitoring and the early detection of emerging viruses. (biomedcentral.com)
Merkel5
  • Recent advances have been made by an accumulation of studies on Merkel cell polyomavirus (MCPyV), which is highly associated and integrated in most Merkel cell carcinomas ( 5 ). (cdc.gov)
  • In 2017, Merkel cell polyomaviruses also were isolated from lesions. (medscape.com)
  • The pathogenesis of 80% of Merkel cell carcinoma (MCC) cases is associated with Merkel cell polyomavirus (MCPyV). (bvsalud.org)
  • Two accepted possible pathways for Merkel cell carcinoma (MCC) pathogenesis include the clonal integration of the Merkel cell polyomavirus (MCPyV) into the neoplastic cells and by UV irradiation. (bvsalud.org)
  • Merkel cell carcinoma (MCC) is a rare cutaneous neuroendocrine carcinoma that is frequently divided into Merkel cell polyomavirus negative and positive tumors due their distinct genomic and transcriptomic profiles, and disease outcomes. (bvsalud.org)
Progressive Multifocal Leukoenc6
  • Detection of HIV-1 Tat and JCV capsid protein, VP1, in AIDS brain with Progressive Multifocal Leukoencephalopathy. (lsuhsc.edu)
  • 9. Evidence for involvement of transforming growth factor beta1 signaling pathway in activation of JC virus in human immunodeficiency virus 1-associated progressive multifocal leukoencephalopathy. (nih.gov)
  • 13. Quantification of human polyomavirus JC in brain tissue and cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy by competitive PCR. (nih.gov)
  • 16. High-resolution melting analysis for mutation scanning in the non-coding control region of JC polyomavirus from patients with progressive multifocal leukoencephalopathy. (nih.gov)
  • 18. Human polyomavirus JC reactivation and pathogenetic mechanisms of progressive multifocal leukoencephalopathy and cancer in the era of monoclonal antibody therapies. (nih.gov)
  • 19. Detection of JC virus in two African cases of progressive multifocal leukoencephalopathy including identification of JCV type 3 in a Gambian AIDS patient. (nih.gov)
Papillomavirus infection1
  • We are also using genetic and biochemical techniques to determine how tumor viruses enter cells and have identified novel cellular factors required for polyomavirus and papillomavirus infection. (yale.edu)
Neoplastic cells1
  • The multifunctional nuclear protein large T-antigen was detectable by immunohistochemical analyses in a subset of neoplastic cells. (cdc.gov)
Tumors4
  • Detection of JC virus DNA sequences and expression of the viral regulatory protein T-Antigen in tumors of the central nervous system. (lsuhsc.edu)
  • Raccoon polyomavirus may contribute to the development of malignant brain tumors of raccoons. (cdc.gov)
  • Furthermore, transgenic mice harboring the viral-encoded large T-antigen (LT-Ag) alone develop tumors of neuroectodermal origin, including malignant peripheral nerve sheath tumors (MPNSTs) and glioblastomas. (cdc.gov)
  • We characterized from these tumors a candidate etiologic agent, raccoon polyomavirus (RacPyV). (cdc.gov)
Localization1
  • Detection of JC polyomavirus DNA sequences and cellular localization of T-Antigen and Agnoprotein in oligodendrogliomas. (lsuhsc.edu)
SV402
Protein6
  • The specific interaction of the Rous sarcoma virus transforming protein, pp60src, with two cellular proteins. (wikipedia.org)
  • Detection of human neurotropic JC virus DNA sequences and expression of the viral oncogenic protein in pediatric medulloblastomas. (lsuhsc.edu)
  • Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. (thermofisher.com)
  • We discovered that the 44-amino acid transmembrane E5 protein of bovine papillomavirus transforms cells to tumorigenicity by binding to and activating the cellular platelet-derived growth factor receptor. (yale.edu)
  • In transformed cells, the E5 protein exists as a dimeric transmembrane protein with a very hydrophobic central domain that spans the membranes of the Golgi apparatus and endoplasmic reticulum. (yale.edu)
  • We discovered that the E5 protein employs a unique mechanism to transform cells. (yale.edu)
Proteins1
  • This cell cycle block was reversed by inactivation of Rb proteins with viral oncoproteins such as polyoma large T (PyLT) antigen and Adenovirus E1A. (silverchair.com)
Oncogenesis1
Sequences1
  • Polyomavirus-specific DNA sequences were detected in 8.3% (29/351) of specimens collected from 220 children. (nih.gov)
Gene product1
  • Expression of human neurotropic polyomavirus JCV late gene product Agnoprotein in human medulloblastoma. (lsuhsc.edu)
Isolation1
  • Studies reliant on molecular detection of tumor-associated virus in isolation, however extensive, are inconclusive because association between PyVs and naturally occurring neoplasms varies and because PyV infections are highly prevalent, yet tumor formation is rare ( 3 , 4 , 12 - 14 ). (cdc.gov)
Expression3
  • Pituitary neoplasia induced by expression of human neurotropic Polyomavirus, JCV, early genome in transgenic mice. (lsuhsc.edu)
  • Expression of JC virus T-Antigen in a multiple sclerosis patient with a Glioblastoma multiforme. (lsuhsc.edu)
  • HN - 2006(1998) MH - Activating Transcription Factor 1 UI - D051697 MN - D12.776.260.108.61.500 MN - D12.776.930.127.61.500 MS - An activating transcription factor that regulates expression of a variety of genes including C-JUN GENES and TRANSFORMING GROWTH FACTOR BETA2. (nih.gov)
Antibodies1
  • The present findings suggest that the detection of immune complexes in unfractionated samples of late tumour-bearer serum using a C1q-binding assay is masked by the increasing production of tumour-specific antibodies and by a shift from complement fixing to non-complement-fixing tumour-specific antibodies. (nih.gov)
Positive3
  • Eleven samples (3.1%) from 8 (3.6%) children were positive for both polyomavirus and EBV. (nih.gov)
  • [ 7 ] In KAs, cells that stain positive with proliferating-cell nuclear antigen immunostaining are distributed only in the outer edges of the tumor, corresponding to the proliferating squamous epithelial cells. (medscape.com)
  • In contrast, cells within an SCC that stain positive with proliferating-cell nuclear antigen immunostaining are more diffusely distributed. (medscape.com)
Tissue2
  • We have identified a candidate etiologic agent, dubbed raccoon polyomavirus, that was present in the tumor tissue of all affected animals but not in tissues from 20 unaffected animals. (cdc.gov)
  • Thus, a number of viral agents are present in tissues and excreta of man and lower animals, which defy detection by methods other than tissue culture. (medicalveritas.org)
Philadelphia2
  • PURPOSE: To evaluate the detection rates of glaucoma-related diagnoses and the initial treatments received in the Philadelphia Glaucoma Detection and Treatment Project, a community-based initiative aimed at improving the detection, treatment, and follow-up care of individuals at risk for glaucoma. (cdc.gov)
  • CONCLUSIONS: Targeting individuals at risk for glaucoma in underserved communities in Philadelphia yielded a high detection rate (39.1%) of glaucoma-related diagnoses. (cdc.gov)
Virus2
  • Identification of a transformation-specific antigen induced by an avian sarcoma virus. (wikipedia.org)
  • Adaptation of lymphadenopathy associated virus (LAV) to replication in EBV-transformed B lymphoblastoid cell lines. (academicinfluence.com)
Early1
  • However, through this method, detection of PCa even at an early stage is not always accompanied by accurate determination of morbidity risk and therefore mortality might result due to over-treatment in some cases and under-treatment in others. (researchsquare.com)
Specific1
  • Prostate specific antigen (PSA) testing followed by trans-urethral resection of the prostate has long been recommended for diagnosis of prostate inflammation, while prognosis of PCa is predicted by Gleason grading, a low-power microscopic evaluation subjected to inter-observer variations and lacks precision 6 , 7 . (researchsquare.com)
Variety1
  • A variety of denoising methods have been proposed to enhance spectroscopic peaks, but still fall short for the detection of low-intensity signals. (nih.gov)