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  • fluorescence
  • The antibody and streptavidin conjugates of Alexa Fluor FluoroNanogold 1.4 nm gold clusters ( Secondary Immunoreagents-Section 7.2 , Avidin, Streptavidin, NeutrAvidin and CaptAvidin Biotin-Binding Proteins and Affinity Matrices-Section 7.6 ) can also be used with hapten-labeled tyramides for correlated fluorescence, light and electron microscopy studies. (thermofisher.com)
  • The antibodies are labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection. (wikipedia.org)
  • This technique was originally developed for the evaluation of the differences between the chromosomal complements of solid tumor and normal tissue, and has an improved resoIution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized. (wikipedia.org)
  • This is achieved through the use of competitive fluorescence in situ hybridization. (wikipedia.org)
  • sequences
  • Phylogenetic analyses of CP amino acid sequences from these begomoviruses revealed that the CP sequences of begomoviruses recognized by the narrow-spectrum MAb M1 were highly conserved, sharing 93% identity with each other but only 72-81% identity with MAb M1-negative begomoviruses. (biomedcentral.com)
  • The CP sequences of begomoviruses recognized by the broad-spectrum MAb D2 demonstrated a wider range of amino acid sequence identity, sharing 78-96% identity with each other and 72-91% identity with those that were not detected by MAb D2. (biomedcentral.com)
  • Thus, MMTV can infect human cells and this finding provides a possible explanation for the detection by others of MMTV sequences in human breast cancer patients. (aacrjournals.org)
  • Detection of sequences with moderate or high similarity depends on how stringent the hybridization conditions were applied-high stringency, such as high hybridization temperature and low salt in hybridization buffers, permits only hybridization between nucleic acid sequences that are highly similar, whereas low stringency, such as lower temperature and high salt, allows hybridization when the sequences are less similar. (wikipedia.org)
  • The global standard library of rRNA sequences is constantly becoming larger and continuously being updated, and thus the possibility of a random hybridization event between a specifically-designed probe (based on complete and current data from a range of test organisms) and an undesired/unknown target organism cannot be easily dismissed. (wikipedia.org)
  • antigen
  • As a consequence of their in vitro manufacturing method the antigen recognition site of synthetic antibodies can be engineered to any desired target and may extend beyond the typical immune repertoire offered by natural antibodies. (wikipedia.org)
  • conjugates
  • 2/ With two competing companies in Europe, Inotech, Basil, Switzerland and Boehringer Mannheim, Germany, Dr. Sutula pioneered the concept of "reagent sets"-matched, quality-controlled antibodies and antibody-enzyme conjugates that can be used to prepare and perform one's own test. (wikipedia.org)
  • Reagents
  • The Zenon Horseradish Peroxidase Antibody Labeling Kits ( Zenon Technology: Versatile Reagents for Immunolabeling-Section 7.3 , Zenon Antibody Labeling Kits-Table 7.7 ), which are described below, are of particular utility when used in combination with TSA technology. (thermofisher.com)
  • Synthetic antibodies are affinity reagents generated entirely in vitro, thus completely eliminating animals from the production process. (wikipedia.org)
  • lectin
  • Glycobiological cancer diagnosis and prognosis relies on lectin or antibody-based glycotope detection, performed either on tumor biopsy material using histochemistry, or in biofluids using solid-phase or insolution immunoassays. (coursehero.com)
  • detectable
  • Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable. (google.ca)
  • Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. (google.ca)
  • 3. The method of claim 1 wherein the antibody reagent is labeled with a detectable chemical group. (google.ca)
  • 6. The method of claim 3 wherein the labeled antibody reagent which becomes bound to said duplexes is separated from that which does not become so bound and wherein the detectable chemical group is measured in one of the separated fractions. (google.ca)
  • Not all women infected with HPV-16/18 have detectable levels of HPV-16/18 antibodies, those who seroconvert develop low antibody levels, and seroconversion occurs typically several months post-infection. (pubmedcentralcanada.ca)
  • In contrast, not all women infected with human papillomavirus (HPV) 16/18 have detectable levels of anti HPV-16/18 antibodies. (pubmedcentralcanada.ca)
  • sensitivity
  • TSA has been reported to increase detection sensitivity up to 100-fold, as compared with conventional avidin-biotinylated enzyme complex (ABC) procedures. (thermofisher.com)
  • pathogen detection
  • In this study, we have developed a singleplex immunoassay for Salmonella to evaluate the potential of SPRi in pathogen detection. (springer.com)
  • Conventional pathogen detection methods, such as microbiological and biochemical identification are time-consuming and laborious, while immunological or nucleic acid-based techniques require extensive sample preparation and are not amenable to miniaturization for on-site detection. (mdpi.com)
  • Digoxigenin
  • Albeit generic, the ligation probe is similar to a detection probe in that it is labelled with, for example, digoxigenin for downstream signalling. (wikipedia.org)
  • commonly used markers are 32P (a radioactive isotope of phosphorus incorporated into the phosphodiester bond in the probe DNA) or Digoxigenin, which is a non-radioactive, antibody-based marker. (wikipedia.org)
  • Optimization of a Digoxigenin-Based Immunoassay System for Gene Detection in Arabidopsis thaliana" (pdf). (wikipedia.org)
  • PCNA
  • Imaging of the nuclear distribution of PCNA (via antibody labeling) can be used to distinguish between early, mid and late S phase of the cell cycle. (wikipedia.org)
  • temperature
  • Dynamic allele-specific hybridization (DASH) genotyping takes advantage of the differences in the melting temperature in DNA that results from the instability of mismatched base pairs. (wikipedia.org)
  • Bacteria
  • As other virulent bacteria, GBS harbors an important number of virulence factors (virulence factors are molecules produced by bacteria that boosts their capacity to infect and damage human tissues), the most important being the capsular polysaccharide (rich in sialic acid) and a pore-forming toxin, β-hemolysin. (wikipedia.org)
  • As other virulent bacteria, GBS harbours an important number of virulence factors, the most important are the capsular polysaccharide (rich in sialic acid), and a pore-forming toxin, β-haemolysin. (wikipedia.org)
  • microbial
  • To reduce the healthcare and economical burdens associated with foodborne infections, real-time and deployable microbial detection and source identification becomes crucial. (springer.com)
  • pathogens
  • Rapid early detection of food contamination is therefore relevant for the containment of food-borne pathogens. (mdpi.com)
  • He has authored several articles and many posters and presentations dealing with the detection of plant pathogens. (wikipedia.org)
  • genetic
  • For example, because cell-free nucleic acids exist in human plasma, a simple blood sample can be enough to sample genetic information from tumours, transplants or an unborn fetus. (wikipedia.org)
  • reaction
  • In 1976, Shmeder had proposed chemiluminescence immunoassay, using the 3-aminophthalhydrazide-H202 and its derivative ABEI as the reaction detection system. (chemicalbook.com)
  • After hybridization, the target nucleic acid is detected through a series of steps that lead to an enzyme‐catalyzed light reaction. (currentprotocols.com)
  • The hybridized nucleic acid is reacted with a transition metal complex capable of oxidizing a preselected base in the hybridized nucleic acid in an oxidation-reduction reaction, the oxidation-reduction reaction is detected, and the presence or absence of the nucleic acid is determined from the detected oxidation-reduction reaction. (google.ca)
  • species
  • The results of begomovirus detection in 147 field samples indicated that MAb M1 reacted with 2 begomovirus species, TYLCTHV and Tobacco leaf curl Yunnan virus (TbLCYnV), whereas MAb D2 reacted with 4 begomovirus species, TYLCTHV, TbLCYnV, Tomato leaf curl New Delhi virus (ToLCNDV) and Squash leaf curl China virus (SLCCNV). (biomedcentral.com)
  • rapid
  • Biosensors have shown tremendous promise to overcome these limitations and are being aggressively studied to provide rapid, reliable and sensitive detection platforms for such applications. (mdpi.com)