STECStaphylococcusPathogenicClostridiumGenetically modifiedKlebsiellaBacterialSalmonellaMicroorganismsPathogensInfectionEpidemiologyJejuniStrainsGeneticContaminationExtracellularHumansPathogen detectionYeastMicrobialPatternsCorrelationMechanismsPrimarilyCellsSpeciesRefersMicrobiologyAgriculturalQuantitativeMicrobiologicalImportantProtocolsSpecificResearchFoodIncludePublicEvolutionTechniquesImpactResultsTest
STEC4
- Shiga toxin-producing Escherichia coli (STEC) is an important public health pathogen, capable of causing hemorrhagic diarrhea and life-threatening kidney failure, particularly in children ( 1 ). (cdc.gov)
- The examples used as the antecedent events are Shiga toxin-producing Escherichia coli (STEC) contamination in the growing environment or elevated Escherichia coli ( E. coli ) levels in agricultural water used for fresh produce production. (cdc.gov)
- Assays for the BAX® System Q7 include: Salmonella, Genus Listeria, Listeria monocytogenes, Escherichia coli O157:H7, Staphylococcus aureus, Campylobacter coli/jejuni/lari, E. sakazakii (Cronobacter), Vibrio cholerae/parahaemolyticus/vulnificus, yeast and mould and coming soon, BAX® Real-Time STEC screening assay and confirmation panels. (rapidmicromethods.com)
- Shiga toxin-producing Escherichia coli (STEC) is a group of human foodborne pathogens transmitted to humans through the consumption of different types of food. (bvsalud.org)
Staphylococcus5
- It can be caused by Staphylococcus aureus toxins, for example. (wikipedia.org)
- Staphylococcus aureus is a ball-shaped bacterium which can be observed as a grape-like clustered organism under the microscope. (r-biopharm.com)
- We collected 77 environmental samples from the surroundings of 12 patients with infection/colonizations by Escherichia coli , Staphylococcus aureus or Klebsiella spp. (mdpi.com)
- The interactions between four pathogenic bacteria ( E. coli , Enterococcus , Staphylococcus aureus , fecal coliforms) isolated from the sewage outfall and the bacterial diversity were investigated in the sea adjacent to the Xinghai sewage outlet, China to illustrate the influence of pathogenic bacteria on the bacterial community structure. (researchsquare.com)
- The bacterial abundance had the most significant correlation with E. coli , followed by Enterococcus and fecal coliforms, and the least with Staphylococcus aureus . (researchsquare.com)
Pathogenic3
- The application of molecular techniques in food is large, several authors comment on the detection and quantification of strains of pathogenic microorganisms present in foods and the presence of toxins and waste allergens not related to specific goods in production lines. (peertechzpublications.com)
- In general, the most known and used methods are Western Blots (WB), Enzyme-Linked Immune Sorbent Assay (ELISA), Lateral-Flow Assay (LFA), and Dot-Blot Assay (DBA) and have been applied in the detection of carcinogenic compounds, pathogenic and allergenic microorganisms using antibodies [8]. (peertechzpublications.com)
- This review summarizes new de-velopments in our understanding of diverse virulence factors associated encoding genes used by different pathotypes of enteric pathogenic E. coli to cause intestinal and extraintestinal diseases in humans. (preprints.org)
Clostridium3
- The botulinum toxin, which is primarily produced by Clostridium botulinum and less frequently by other Clostridium species, is the most toxic substance known in the world. (wikipedia.org)
- Most clostridium species that do have toxins typically have binary toxins with the first unit involved in getting the toxin into the cell and the second unit cause cellular stress or deformation. (wikipedia.org)
- Toxin A and toxin B are two toxins produced by Clostridium difficile. (wikipedia.org)
Genetically modified3
- Our teams monitor, among other things, the quality of biological medicinal products for human and veterinary use (vaccines and blood products) before they are placed on the market, assess and monitor the quality of laboratories, or assess the risks related to the use of genetically modified organisms ( GMO s) and/or pathogens. (sciensano.be)
- They are also used to control the authenticity of many foods, very important for receiving raw material industries, identification of genetically modified organisms, and genetic selection in yeast for fermentation processes. (peertechzpublications.com)
- Molecular techniques have facilitated the identification, genotyping, and monitoring of food contamination sources, identification of genetically modified organisms, and use as food detection methods to avoid falsification through genetic markers. (peertechzpublications.com)
Klebsiella1
- Globally, multi-drug resistant (MDR) bacteria were estimated to cause 4.95 (3.62-6.57) million deaths a year, with third-generation cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae among the leading causes of MDR deaths worldwide [ 1 ]. (biomedcentral.com)
Bacterial6
- Currently, new methods of detecting bacterial toxins are being developed to better isolate and understand these toxins. (wikipedia.org)
- Some bacterial toxins can be used in the treatment of tumors. (wikipedia.org)
- Toxinosis is pathogenesis caused by the bacterial toxin alone, not necessarily involving bacterial infection (e.g. when the bacteria have died, but have already produced toxin, which are ingested). (wikipedia.org)
- Pore forming bacterial toxins are common and have a very interesting way of entering or invading the host's cell. (wikipedia.org)
- Our objective was to compare genomes of E. coli serogroup O26 isolates from human clinical cases and cattle in New Zealand with genomes of bacterial isolates from non-New Zealand sources, examining the genetic diversity and population structure, evolution, time to most recent common ancestor (tMRCA), antimicrobial resistance, and virulence genes. (cdc.gov)
- Fecal grab samples were collected before and after treatment (weeks 1, 2, 3, 5, 7, and 9) for bacterial quantification and metagenomic next-generation sequencing. (biomedcentral.com)
Salmonella3
- Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp. (iso.org)
- Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp. (iso.org)
- Amendment 1: Annex D: Detection of Salmonella spp. (iso.org)
Microorganisms2
- Current research themes include microbiome analysis of biomass-degrading microorganisms for biofuels and biotechnology, high-throughput methods for the detection of human pathogens in aquatic environments, and microbiome analysis of the complex tree disease, Acute Oak Decline. (bangor.ac.uk)
- Abstract: Escherichia coli are remarkably versatile microorganisms and important members of the normal intestinal microbiota of humans and animals. (preprints.org)
Pathogens2
- Along with the RiboPrinter® system, DuPont Qualicon markets the award-winning BAX® system, an innovative DNA-based technology for detecting pathogens and other organisms in food and environmental samples. (rapidmicromethods.com)
- There are two formats in each immunoassay test: 1) sandwich format (for macromolecule detection, such as foodborne pathogens and allergen proteins). (peertechzpublications.com)
Infection3
- Many microbial toxins promote infection and disease by directly damaging host tissues and by disabling the immune system. (wikipedia.org)
- Lethal toxins refers to the group of toxins that are the obvious agents responsible for death associated with the infection. (wikipedia.org)
- lt;span lang="EN-US">This review presents the basic principles of application of the loop-mediated isothermal amplification (LAMP) reaction for the rapid diagnosis of coronavirus infection caused by SARS-CoV-2. (crie.ru)
Epidemiology2
Jejuni1
- From approximately 15 known Campylobacter species, mainly C. jejuni and C. coli are known to be causes for human gastroenteritis. (r-biopharm.com)
Strains1
- there are some strains of Corynebacterium diphtheriae that do not produce diphtheria toxin and are considered nonvirulent and nontoxigenic. (wikipedia.org)
Genetic2
- Genetic makeup of Shiga toxin-producing Escherichia coli in relation to clinical symptoms and duration of shedding: a microarray analysis of isolates from Swedish children. (cdc.gov)
- This harmless commensal organism can acquire a mixture of comprehensive mobile genetic elements that contain genes encoding viru-lence factors, becoming an emerging human pathogen capable of causing a broad spectrum of intestinal and extraintestinal diseases. (preprints.org)
Contamination1
- It is possible to assess the risk of contamination via quantitative detection to ensure that, if necessary, preventive measures can be taken. (r-biopharm.com)
Extracellular1
- Clostridial toxins have the ability to damage or alter the cell membrane damaging the extracellular matrix of the organism. (wikipedia.org)
Humans2
- Clostridial toxins are widespread and aid in the production of many diseases in humans and other organisms. (wikipedia.org)
- Gene variation among isolates from humans, animals, and food was strongly associated with country of origin and stx toxin profile but not isolation source. (cdc.gov)
Pathogen detection2
- An RCA may be conducted in response to a foodborne outbreak caused by a contaminated product, a product recall from pathogen detection during finished product testing, detection of a pathogen on the pre-harvest product or within the growing environment, or elevated E. coli levels in agricultural water. (cdc.gov)
- DuPont Qualicon science-based systems for pathogen detection and microbial monitoring, together with Danisco Care4U™ range of natural food protection solutions, help companies protect their products, productivity and brands. (rapidmicromethods.com)
Yeast2
- PCP's preservatives were chemically neutralized leading to the yeast and mold recovery by means of the alternative microbiological method (AMM) and the pour-plate method. (bvsalud.org)
- Thus, this new technology was found to meet all the validation criteria needed to be considered for an alternative method for yeast and mold quantification in the PCPs tested. (bvsalud.org)
Microbial2
- However, microbial toxins also have important uses in medical science and research. (wikipedia.org)
- Potential applications of toxin research include combating microbial virulence, the development of novel anticancer drugs and other medicines, and the use of toxins as tools in neurobiology and cellular biology. (wikipedia.org)
Patterns1
- New Zealand's remarkably fewer introductions of Shiga toxin-producing Escherichia coli O26 compared with other countries (such as Japan) might be related to patterns of trade in live cattle. (cdc.gov)
Correlation1
- An equivalence of results was made through the construction of correlation curves that allowed the establishment of numerically equivalent results between the enumeration data from the reference method (CFU) and the alternative method (Detection times, DTs). (bvsalud.org)
Mechanisms2
- Clostridial toxins are known to aid in gastrointestinal diseases and there is a wide range of mechanisms that clostridial toxins take to invade or enter the cell of the host. (wikipedia.org)
- The main authors of the theme defend the use of these techniques to improve the quality assurance of foods, in addition to studying the molecular mechanisms of the main contaminants. (peertechzpublications.com)
Primarily1
- The advantages and limitations of this promising methodology in comparison to other methods of molecular diagnostics, primarily RT-PCR, are discussed, as well as the prospects for the development of technology for the detection of other infectious agents. (crie.ru)
Cells1
- The mechanism that clostridial toxins follow includes clostridia forming pores and then the pores inserting themselves into the cell membrane of cells. (wikipedia.org)
Species1
- Some of these species produce harmful toxins such as botulinum toxin and tetanus toxin among others. (wikipedia.org)
Refers1
- Immunoassay refers to a class of analytical methods based on antigen-antibody-specific interactions. (peertechzpublications.com)
Microbiology1
- You can also follow our news posts in the Rapid Microbiology Methods LinkedIn group . (rapidmicromethods.com)
Agricultural1
- Insights into how this effector-immunity pairing alters the physiological responses of the recipient organism might provide opportunities relating to the T6SS agricultural and biotherapeutic applications. (preprints.org)
Quantitative1
- The main purpose of this validation study was to prove that the alternative method's entire performance is not inferior to the conventional pour-plate method for a quantitative determination of yeasts and molds. (bvsalud.org)
Microbiological1
- Their detection is mainly performed by targeting specific serogroups by classical microbiological methods and, later, by molecular typing with different techniques. (bvsalud.org)
Important2
- Shiga toxin-producing Escherichia coli serogroup O26 is an important public health pathogen. (cdc.gov)
- Therefore, effective quality control with reliable test methods is important. (r-biopharm.com)
Protocols2
- Methods and Protocols. (bangor.ac.uk)
- Studies have been demonstrating the effectiveness of molecular techniques regarding the accuracy of the protocols used and the agility to provide the necessary answers. (peertechzpublications.com)
Specific2
- Exotoxin activity can be separated into specific cytotoxic activity or broad cytotoxic activity based on whether the toxin targets specific cell types or various cell types and tissues, respectively. (wikipedia.org)
- The basic technical details of the method, and the most popular approaches of specific and non-specific detection of amplification products are briefly described. (crie.ru)
Research2
Food3
- The BAX® System allows food laboratories to quickly and accurately detect organisms in raw ingredients, finished products and environmental samples. (rapidmicromethods.com)
- This review aims to address the use of molecular techniques applied to the identification of food. (peertechzpublications.com)
- For a long time, food analysis has always been based on physical, chemical, physicochemical, and biochemical methods, however, these are techniques that sometimes require a lot of time and have a low degree of precision. (peertechzpublications.com)
Include1
- Additional classifications used to describe toxins include enterotoxin, neurotoxin, leukocidin or hemolysin which indicate where in the host's body the toxin targets. (wikipedia.org)
Public1
- The primary method of dissemination will be existing and future public data repositories (e.g. (nimml.org)
Evolution1
- The large amount of sequence data produced by such initiatives as GenomeTrakr ( 8 ) provides an opportunity to interpret the evolution and transmission of organisms across national boundaries. (cdc.gov)
Techniques1
- New diagnostic techniques are urgently needed for the independent, rapid and accurate detection of Mycobacterium tuberculosis to guide the diagnosis of TBM. (bvsalud.org)
Impact1
- Assessing the Impact of Assemblers on Virus Detection in a De Novo Metagenomic Analysis Pipeline. (cdc.gov)
Results4
- RESULTS: Thirty PCPs have been tested for quantification of yeasts and molds using an AMM. (bvsalud.org)
- CONCLUSION: It was shown that all the test results obtained from the alternative method were in statistical agreement with the standard plate-count method (PCM). (bvsalud.org)
- The methods presented in this review proved to be fast, and effective and could be effective substitutes for chemical and physical methods, chemical and biochemical, producing results with greater accuracy, reliability and speed of analysis. (peertechzpublications.com)
- Instrumental analyses, commonly used in physical-chemical methods, present systematic errors related to the equipment and reagents used, as well as errors in reading, and weighing by the operator, and require several hours before the results are obtained. (peertechzpublications.com)
Test1
- METHODS: C. albicans and A. brasiliensis were pooled to use as inoculum (equivalent to 1.0 × 108 CFU/mL) in the suitability of the method test. (bvsalud.org)