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  • STEC
  • Together these findings highlight improved strategies for STEC detection using currently available systems and the development of novel approaches for future surveillance. (frontiersin.org)
  • Growing recognition of the shortfall in STEC detection has prompted a shift toward more comprehensive STEC identification methods. (frontiersin.org)
  • This research addressed three important components for detection of the recently declared STEC adulterant serotypes. (k-state.edu)
  • Part three, part of a field study determining STEC-7 prevalence rates for cattle, hides and dressed carcasses, compared qualitative results generated by two molecular-based detection systems (BioControl Assurance GDS® and Neogen NeoSeek™), analyzing 576 selectively enriched beef carcass sponge samples collected from a commercial processing facility. (k-state.edu)
  • It was speculated the higher GDS prevalence rate was due to false-positive determinations from the mixed culture enrichments, as viable STEC-7 cells were not recovered by immuno-magnetic bead culture isolation. (k-state.edu)
  • matrices
  • Founded in 2007 by researchers from the University of New Mexico, nanoMR is an early-stage life sciences company developing novel diagnostic systems for the rapid isolation of rare cell types, including bacterial and fungal pathogens, directly from blood and other matrices. (andhranews.net)
  • assay
  • Antonarakis and colleagues used a CTC-based AR-FL assay, including selection and detection kits. (onclive.com)
  • Expectantly, a thorough review of the current knowledge and technology of CTC detection will assist the scientific community in the development of more efficient CTC assay systems. (thno.org)
  • mRNA
  • AR-FL mRNA levels were also associated with detection of AR-V7-previously shown to predict lack of response to abiraterone acetate (Zytiga) and enzalutamide (Xtandi)-and with a lower PSA response in patients treated with either of the AR-targeting drugs. (onclive.com)
  • The detection kit permitted mRNA purification and use of multiplex RT-PCR probes for PSA, prostate-specific membrane antigen, EGFR , and actin. (onclive.com)
  • methods
  • These methods enable the concentration of pathogens present in the initial matrix into smaller volume and facilitate the isolation of sufficient quantities of pure DNA. (hindawi.com)
  • However, this study was based in the US and the results may not be directly applicable to Great Britain since there are differences in the methods used for isolation. (royalsocietypublishing.org)
  • Immunocytochemistry-based methods for the detection of BM micrometastases did not provide any information about the clinical status of patients, but helped to refine the immunomagnetic data by confirming the presence of micrometastases in some cases. (biomedcentral.com)
  • The methods optimised and assessed in this study proved to be suitable for the detection and isolation of E. coli O157:H7 from environmental water, food and faecal samples. (up.ac.za)
  • In addition to the isolation of E. coli O157:H7 from these sources, methods were also optimised for the characterisation of E. coli O157:H7 using repetitive sequence analysis and induction of shiga toxin (Stx)¬converting phages which carry the genes coding for Stx from strains of E. coli O157:H7. (up.ac.za)
  • clinical
  • The early detection of Johne's disease is difficult because of the long incubation period, which can last 2-10 years before clinical symptoms appear [ 3 ]. (hindawi.com)
  • These categories were highly correlated with the recently revised American Joint Committee on Cancer staging system for breast cancer, demonstrating the clinical relevance of this simple and reliable immunomagnetic technique. (biomedcentral.com)
  • pigs
  • The validity of pooled sampling for detection of Salmonella in pigs has not been established and there is an urgent need to do so, since advice to individual farmers and to the industry as a whole will be based on these results. (royalsocietypublishing.org)
  • Cell
  • In addition, the potential of tumor cell-derived materials as new targets for CTC detection, including circulating tumor microemboli, cell fragments, and circulating DNA, is evaluated. (thno.org)
  • A low-cost single-cell isolation system incorporating a digital versatile disc burner (DVD RW) optical pickup has been developed. (osapublishing.org)
  • The concept of circulating tumor cell (CTC) detection has so far been put forward for breast cancer in particular. (biomedcentral.com)
  • presence
  • However, the extraction of DNA which is a crucial step prior to PCR detection can be complicated due to the presence of inhibitory substances. (hindawi.com)
  • complicated due
  • Isolation of A. pleuropneumoniae from nasal cavities or tonsils from carrier animals is complicated due to the mixed bacterial flora present. (dtu.dk)