AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
In Situ HybridizationIn Situ Hybridization, FluorescenceNucleic Acid HybridizationRNA, MessengerMolecular Sequence DataDNA ProbesBase SequenceRNA ProbesHybridization, GeneticImmunohistochemistryPolymerase Chain ReactionDigoxigeninCloning, MolecularGene ExpressionOligonucleotide ProbesChromosome AberrationsBlotting, NorthernChromosome MappingAmino Acid SequenceComparative Genomic HybridizationGene AmplificationKaryotypingDNA, ComplementaryGene Expression Regulation, DevelopmentalChromosome BandingTranslocation, GeneticCarcinoma in SituSequence Analysis, DNAPhylogenyBlotting, SouthernRNA, Ribosomal, 16SDNAAneuploidyReverse Transcriptase Polymerase Chain ReactionDNA, RibosomalTissue DistributionDNA, BacterialGene DosageOligonucleotide Array Sequence AnalysisPrimed In Situ LabelingSequence Homology, Amino AcidTranscription, GeneticDNA PrimersDNA, ViralChromosomes, Human, Pair 1Chromosomes, Human, Pair 17Gene LibraryParaffin EmbeddingChromosomes, Human, Pair 7Nucleic Acid ProbesSpecies SpecificityGene Expression RegulationGene Expression ProfilingOrgan SpecificityRNACytogenetic AnalysisChromosome DeletionChromosomes, Human, Pair 11Peptide Nucleic AcidsSequence Homology, Nucleic AcidGenes, erbB-2Chromosomes, Human, Pair 12GenesChromosomes, Human, Pair 8Y ChromosomeBrainInterphaseChromosomes, HumanSensitivity and SpecificityAutoradiographyCytogeneticsEmbryonic and Fetal DevelopmentChromosome PaintingGene RearrangementMolecular Probe TechniquesChromosomes, Human, Pair 14Chromosomes, Human, Pair 18Rats, Sprague-DawleyPhenotypeChromosome DisordersSequence AlignmentChromosomes, Artificial, BacterialRNA, ViralImmunoenzyme TechniquesChromosomesTranscription FactorsRestriction MappingChromosomes, Human, Pair 3Tissue FixationChromosomes, Human, Pair 13RNA, BacterialMetaphaseCells, CulturedZebrafishDNA, NeoplasmTrisomyReceptor, erbB-2Antisense Elements (Genetics)Embryo, NonmammalianEmbryo, Mammalian
FluorescenceCISHAssaysImmunohistochemistryReagentsProbesDenaturation and hybridizationFluorescentDetectionNucleic acidRNAScopeProbeFISHHER2DiagnosticsQuantitativeTissue2020ViralAbstractTissuesSignalsBiopsiesAntibodyTechniqueMethodFindingsProductGene expressionCellsWideEasyCancerSmallSolutionMeasureCheck
Fluorescence14
  • It was developed around the year 2000 as an alternative to fluorescence in situ hybridization (FISH) for detection of HER-2/neu oncogene amplification. (wikipedia.org)
  • FISH probes are generally labelled with a variety of different fluorescent tags and can only be detected under a fluorescence microscope, whereas CISH probes are labelled with biotin or digoxigenin and can be detected using a bright-field microscope after other treatment steps have been applied. (wikipedia.org)
  • Early in 2020, Vizgen, a Harvard University spinout looking to commercialize the multiplexed, error-robust fluorescence in situ hybridization (MERFISH) method, launched with $14 million in Series A financing . (genomeweb.com)
  • Instead of direct fluorescence labelling of the probe, various haptens - digoxigenin, biotin, dinitrophenyl - are used which are detected in a multi-stage procedure by means of chromogenic precipitate formation. (zytomed-systems.com)
  • DNA fluorescence in situ hybridization (FISH) technology is used to study chromosomal and genomic changes in fixed cell suspensions and tissue block preparations. (biomedcentral.com)
  • The fluorescence in Situ Hybridization or FISH check detects extra copes of the involved HER2 genes if any in these cells. (healthtracking.us)
  • Whether you select to visualize your RNA and DNA targets by fluorescence (FISH) or chromogenic (CISH) detection, you can be sure to find the best products and guidance for your workflow with our specialists. (ahdiagnostics.dk)
  • Several of them are with both fluorescence tag and chromogenic tag to support your preferred choice of method. (ahdiagnostics.dk)
  • Her-2/ neu gene amplification is primarily detected by in situ hybridization and uses fluorescence (FISH) to detect the signals. (biomedcentral.com)
  • Based on Technology, it is categorized into fluorescence in situ hybridization (FISH) and chromogenic in situ hybridization (CISH). (envisageresearch.com)
  • Hybridizer is a hands-free denaturation and hybridization instrument designed for slide-based fluorescence and chromogenic in situ hybridization (FISH and CISH, respectively) on histology and cytology samples. (dssimage.com)
  • What is Fluorescence In Situ Hybridization (FISH), and how does it work? (dssimage.com)
  • Fluorescence in situ hybridization (FISH) was carried out on FFPE sections with FOXO1/PAX3 and/or FOXO1/PAX7 probes. (aetox.com)
  • Genome wide association studies, fluorescence in situ hybridization, comparative genomic hybridization, and candidate gene studies have enumerated genetic contributors to cancers in women. (hindawi.com)
CISH10
Assays2
  • These assays are now routinely used for monitoring or detecting, as well as to help decide which therapies would work best for patients. (enzolifesciences.com)
  • And in the case of ACD's popular chromogenic ISH assays, it is also necessary to block the activity of endogenous peroxidases. (bio-techne.com)
Immunohistochemistry3
  • Gene amplification and protein overexpression of HER-2orneu in human extrahepatic cholangiocarcinoma as detected by chromogenic in situ hybridization and immunohistochemistry: its prognostic implication in node-positive patients. (kbsmc.co.kr)
  • Subsequently, we investigated if immunohistochemical strategy might detect surrogate marker genes as indicators of fusion gene-positive RMS.45 RMS sufferers have been included within the evaluation and immunohistochemistry was utilized to FFPE tissues collected at prognosis. (aetox.com)
  • Immunohistochemistry (IHC) is a technique that detects antigens in tissue sections by immunological and chemical reactions. (creativebiolabs.net)
Reagents4
  • All RNA-ISH kits contain reagents in a convenient ready-to-use (RTU) format, and chromogenic dyes are available to interrogate a single or two genes simultaneously. (selectscience.net)
  • DSS offers a complete solution for FISH from reagents, probes, hybridization chambers to FL microscopes with accurate filters and software. (dssimage.com)
  • The proprietary formulations of these reagents have been optimized to provide enhanced access of in situ hybridization probes to nucleic acid targets across a wide variety of tissue sample types including FFPE, fresh‑frozen (FF), and fixed frozen tissue as well as tissue microarray (TMA) and cultured adherent and non-adherent cells. (bio-techne.com)
  • It is necessary to check the relevant reagents such as buffers and chromogenic agents, and expiration dates, storage parameters, pH, and order of use are all included. (creativebiolabs.net)
Probes4
  • The technique is based on specific hybridization of small labeled DNA fragments, the probes, to complementary sequences in a target DNA molecule. (biomedcentral.com)
  • Gene probes are used in various blotting and in situ hybridization (ISH) techniques for the detection of nucleic acid sequences in food industry, environmental, medical, and veterinary applications to improve the specificity of the analyses. (enzolifesciences.com)
  • FISH is a molecular technique that uses fluorescent probes that bind to specific parts of the chromosome which is used to detect and localize the presence or absence of specific DNA sequences. (dssimage.com)
  • Following appropriate sample preparation, the next step in solving the puzzle is to provide sufficient access for in situ hybridization probes to their targets. (bio-techne.com)
Denaturation and hybridization1
  • They are complementary to the targeted sequence and bind to it after a denaturation and hybridization step. (wikipedia.org)
Fluorescent4
  • In November, BioTechne reported a surge in demand for the hybridization-based product , which can use both fluorescent and chromogenic dyes, driving an 18 percent rise in fiscal year 2021 first quarter revenues. (genomeweb.com)
  • Guidance on best practices in preparing each of the above sample types for assay categories (chromogenic, fluorescent, multiplexed, automated) is available on the ACD website. (bio-techne.com)
  • RNAscope Protease III Reagent: A moderate protease treatment used with the chromogenic kits for cultured cells and with the fluorescent kits for fixed frozen tissue as well as cultured cells. (bio-techne.com)
  • RNAscope Protease IV Reagent: A strong protease treatment used with both the chromogenic and fluorescent kits for fresh-frozen tissue. (bio-techne.com)
Detection1
Nucleic acid2
  • In Situ Hybridization (ISH) is a powerful technique that allows you to identify and localize nucleic acid targets in formalin-fixed tissue sections and cells. (ahdiagnostics.dk)
  • In situ hybridization is a technique which helps in precisely localization of particular segment of nucleic acid in a histologic section. (envisageresearch.com)
RNAScope4
  • High-resolution, single-molecule, in situ analysis methods, including BioTechne's ACD RNAScope, which detects up to 12 genes at once, have been around for several years. (genomeweb.com)
  • The ACD in situ hybridization kits (RNAscope, BaseScope™, miRNAscope™, and DNAscope™) can be used with formalin-fixed, paraffin-embedded (FFPE) samples, fresh-frozen (FF) or fixed frozen tissues, and both adherent and non-adherent cultured cells. (bio-techne.com)
  • RNAscope Hydrogen Peroxide Reagent: For blocking endogenous peroxidase activity with use of the chromogenic kits. (bio-techne.com)
  • RNAscope Protease Plus Reagent: A mild protease treatment used with the chromogenic kits for FFPE and fixed frozen tissue. (bio-techne.com)
Probe3
  • The degree of homology between target and probe results in stable hybridization. (enzolifesciences.com)
  • In developing a probe, a sequence of nucleotides must be identified, isolated, reproduced in sufficient quantity, and tagged with a label that can be detected. (enzolifesciences.com)
  • The DNA or RNA probe is used in detecting complementary genetic material in tissues or cells. (envisageresearch.com)
FISH2
  • Other techniques that are developed to detect chromosomal abnormalities are usually compared to the sensitivity and specificity of FISH to see how they measure up. (wikipedia.org)
  • HyperChrome is the perfect Instrument for denaturation & hybridization procedures during FISH experiments. (dssimage.com)
HER21
  • Language": "en", "Country": "XG", "Code": "Storage Conditions (Product)" }, { "Name": "Background Information", "Value": "Human epidermal growth factor receptor 2 (HER2) is a member of the epidermal growth factor subfamily of transmembrane receptor tyrosine kinases that mediate the growth, differentiation, and survival of cells. (roche.com)
Diagnostics1
  • Although RT-PCR is widely used to detect and diagnose coronavirus in human samples, researchers and diagnostics companies across the globe are finding cost-effective and sensitive assay kits to deal with the pandemic. (envisageresearch.com)
Quantitative1
  • Methods and results: Paired bladder and cervix carcinoma specimens from 37 patients with cervical carcinoma with bladder involvement, four patients with bladder carcinoma with uterine cervical involvement and two patients with double primaries were studied with quantitative multiplex polymerase chain reaction and chromogenic in-situ hybridization. (tmu.edu.tw)
Tissue1
  • The tissue samples must then be washed and heated several times to remove any paraffin before the hybridization step. (wikipedia.org)
20201
  • Global in situ hybridization market is valued USD 0.79 billion in 2020 and expected to reach USD 1.32 billion by 2027, registering a substantial CAGR of 7.6% in the forecast period of 2020-2027. (envisageresearch.com)
Viral3
  • We detected HCirV-1 transcripts in 2% of hepatocytes, demonstrating viral replication and supporting the role of HCirV-1 in liver damage. (cdc.gov)
  • Among transplant recipients, it is especially critical to detect infection, often viral, to prevent unnecessary interruption of treatment. (cdc.gov)
  • We determined the full-length proviral genomes from six of the seven investigated animals and, using in situ hybridization, identified viral RNA in the cytoplasm of cells morphologically compatible with neurons in diseased brains. (biomedcentral.com)
Abstract1
  • Abstract Anaplasma marginale and A. platys were detected and characterized (16S rDNA sequence analysis) from dairy and indigenous cattle, and the latter in domestic dogs in Vietnam. (edu.vn)
Tissues1
  • It uses specific binding between antibodies and antigens and chromogenic color development to detect and localize specific antigens in tissues. (creativebiolabs.net)
Signals1
Biopsies2
  • Twenty-three tongue biopsies were detected immunohistochemically for p16, Epstein-Barr virus (EBV) and Ki67, and real-time PCR and chromogenic in-situ hybridization for human papillomavirus (HPV). (uwi.edu)
  • Self-examination of the breasts, ultrasound testing, mammography and biopsies are some of the ways to detect this disease. (healthresource4u.com)
Antibody1
Technique2
  • Similar to solving a jigsaw puzzle, successfully performing the technique of in situ hybridization (ISH) benefits from a systematic, step-wise approach that concentrates on one stage of the process at a time, then moves onto the next. (bio-techne.com)
  • The technique is highly sensitive and specific and can detect a wide range of antigens in many animal species. (creativebiolabs.net)
Method2
Findings1
  • Our findings suggest that chromogenic reporter strains can be applied for simple screening, thus reducing the number of samples requiring analytical techniques. (springeropen.com)
Product2
  • HRP then converts diaminobenzidine (DAB) into an insoluble brown product, which can be detected in a bright-field microscope under 40- to 60-fold magnification. (wikipedia.org)
  • This market segments the global in situ hybridization market based on product, technology, application, end user, and region. (envisageresearch.com)
Gene expression1
  • In October, 10x said it would buy ReadCoor, an in situ multiomics platform developed at George Church's Harvard lab, for $350 million as well as Cartana, a Swedish firm offering spatial gene expression analysis methods, for $41.2 million, likely with the intent to combine their technologies into a new platform . (genomeweb.com)
Cells1
  • Using bacterial cells as reporter strains represents an advantage for detecting pollutants present in soil or water samples. (springeropen.com)
Wide1
  • In situ hybridization has wide applications in microbiology, pathology, developmental biology, karyotyping & phylogenetic analysis and physical mapping. (envisageresearch.com)
Easy3
  • Abbott Molecular ThermoBrite® System offers an easy, safe, system for in-situ hybridization procedures. (dssimage.com)
  • Chromoproteins are easy to detect reporter proteins. (springeropen.com)
  • recA -AmilCP expression is easy to detect reporter strain for assessing DNA damage. (springeropen.com)
Cancer3
Small1
Solution1
  • 4-Nitrophenyl-α-D- glucopyranoside is a chromogenic substrate for alpha-D-glucosidase, yielding a yellow solution upon cleavage. (goldbio.com)
Measure1
  • The presence of pollutants in soil and water has given rise to diverse analytical and biological approaches to detect and measure contaminants in the environment. (springeropen.com)
Check1