• The antibody detection method - enzyme-linked immunosorbent assay (ELISA) is especially relevant when examining imported unvaccinated livestock. (journalveterinariya.ru)
  • The OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals recommends the use of four serological tests to rule out brucellosis in international animal trade: the buffered Brucella antigen tests (rose bengal test and buffered plate agglutination test), the complement fixation test (CFT), the enzyme-linked immunosorbent assays (ELISA) and fluorescence polarization assay (FPA). (journalveterinariya.ru)
  • Enzyme-linked immunosorbent assay (ELISA) was for IL-6 levels in peritoneal fluid and cell culture supernatant. (endometriosi.it)
  • Our pre-activated HRP-NHS ester is ideal for labeling antibodies with HRP enzymes in a simple and efficient manner for use in ELISA and other immunoassay applications. (aatbio.com)
  • Overall, our data supports PIF's endogenous self sustaining role in embryo development and the utility of PIF- ELISA to detect viable embryos in a non-invasive manner. (biomedcentral.com)
  • Polarization allows the machine to detect the difference between antibody bound, and unbound fluorescein. (wikipedia.org)
  • We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)
  • This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • A combination of HS201-PDT with anti-PD-L1 antibody demonstrated the greatest antigen-specific immune response, tumor growth suppression, prolonged mouse survival time and abscopal effect. (bmj.com)
  • We examined factors associated with PCP beliefs about screening efficacy and uncertainty and whether beliefs were associated with prostate specific-antigen (PSA) test use. (cdc.gov)
  • Prostate-specific antigen ranges from a mass screening program utilizing extremely delicate RIA kits. (diastrip.com)
  • BACKGROUND This examine was designed to guage the distribution of prostate-specific antigen (PSA) ranges of males in a mass screening program for prostatic illness. (diastrip.com)
  • The SH2-PLA assay utilizes oligonucleotide-conjugated anti-GST and anti-EGFR antibodies recognizing a GST-SH2 probe and cellular EGFR, respectively. (biomedcentral.com)
  • Iron nanoparticles are conjugated with two types of antibodies (one against antigens on therapeutic cells and the other directed at injured cells) to produce magnetic bifunctional cell engager (MagBICE). (regenerativemedicine.net)
  • In addition, different exposure times were explored during the fluorescence detection. (bvsalud.org)
  • An appropriate increase in exposure time also allowed the detection of many weak fluorescent particles that were previously undetectable, indicating increased sensitivity of the fluorescence detection. (bvsalud.org)
  • The assay detected signal across at least 3 orders of magnitude of lysate input with a linear range spanning 1-2 orders and a low femtomole limit of detection for EGFR phosphotyrosine. (biomedcentral.com)
  • The obvious benefit of this method is that the low sample requirement allows detection of SH2 binding in samples which are difficult to analyze using traditional protein interaction assays. (biomedcentral.com)
  • AAT Bioquest are well known for their photometric detection fluorescence and luminescent technologies. (stratech.co.uk)
  • The Raman-active reagents can function as an alternative to fluorescence-labeled reagents, with advantages in detection including signal stability, sensitivity, and the ability to simultaneously detect several biological materials. (patsnap.com)
  • Signal is detected either by chemiluminescence (far-Western and rosette) or by PCR (OTM). (biomedcentral.com)
  • There are three assay platforms for SH2 profiling: quantitative far-Western, rosette, and oligonucleotide-tagged multiplex (OTM) assays [ 10 ]. (biomedcentral.com)
  • This method can be used in beads/spots-based immunoassay in gel, immuno-electrophoresis, and fluorescent staining of protein/nucleic acid bands in gel electrophoresis. (bvsalud.org)
  • Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
  • In-Cell Western assay was used for protein expression of CCL17 and phosphorylation levels of ERK, JNK, and P38. (endometriosi.it)
  • CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. (thermofisher.com)
  • Flow cytometry was applied to detect the expression of CCR4, IL-6, and the phosphorylation levels of NF-κB. (endometriosi.it)
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. (thermofisher.com)
  • citation needed] The current in vitro testing utilizes molecule specific methods of detecting salicylates. (wikipedia.org)
  • We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays that utilizes purified Src Homology 2 (SH2) domains as a molecular tool to profile the global tyrosine phosphorylation state of cells. (biomedcentral.com)
  • Cambridge Life Sciences Ltd - A leading manufacturer of in-vitro diagnostic assays" (PDF). (wikipedia.org)
  • This two-stage amplification closed-tube diagnostic assay was used to test SARS-CoV-2 samples with significantly enhanced sensitivity compared to conventional RT-PCR with dynamic range of at least seven orders of magnitude (2 × 10 −4 -2000 TCID 50 /reaction) for RNA from SARS infected cells and below 10 copies per reaction for DNA plasmid as positive standards [ 1 ] . (encyclopedia.pub)
  • Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays. (lookformedical.com)
  • After image processing and calculations, 27.8% of the average grayscale intensity of the fluorescent spot was retained, comparing the average gray value of the bright spot before and after EE, and 97.6% of excessive FITC in the channel was cleared, obtained by calculating the decreased background fluorescence grayscale intensity after EE. (bvsalud.org)
  • FP Fluor™ superior fluorescent labelling dyes, optimised for developing fluorescence polarisation-based assays. (stratech.co.uk)
  • These professional antigen-presenting cells are able to discriminate unmodified self from non-self thanks to pattern recognition receptors such as the Toll like Receptors (TLR) and Scavenger Receptors (SR). SR were originally identified by their ability to bind and internalize modified lipoproteins and microorganisms but also particles and TLR agonists. (biomedcentral.com)
  • The Raman-active reporter molecule and the binding molecule are affixed to the particle to give both a strong SERS signal and to provide biological functionality, i.e. antigen or drug recognition. (patsnap.com)
  • In this case, the basic principle is to detect specific DNA and RNA sequences via hybridization of a probe, detecting any complementary sequence on the cDNA of this bacterium. (australiaessays.com)
  • DC are professional antigen-presenting cells that are essential for initiating adaptive immune responses. (biomedcentral.com)
  • One limitation of current SH2 profiling methods is that these assays themselves do not provide the identity of the binding proteins. (biomedcentral.com)
  • Raman spectroscopy systems include such NERS-active structures, and methods for performing Raman spectroscopy include irradiating an analyte proximate such a NERS-active structure and detecting Raman-scattered radiation scattered by the analyte. (patsnap.com)
  • We showed for the first time that interactions between SH2 domain probes and EGFR in cell lysate can be determined in a microliter-scale assay using SH2-PLA. (biomedcentral.com)
  • Cell Meter™ cellular functional assay kits, a complete set of tools for functional analysis of cellular events. (stratech.co.uk)
  • We therefore investigated whether oleandrin or a N. oleander extract could inhibit virus transmission from a GFP-expressing HTLV-1+ lymphoma T-cell-line to huPBMCs in co-culture assays. (longdom.org)
  • CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. (thermofisher.com)
  • The virus was detected almost simultaneously in cattle in many countries of the world: USA (1998), Great Britain (2008), Canada (2010), Belgium (2013), Poland (2013), Russia (2013), New Zealand (2014). (journalveterinariya.ru)
  • A device that uses specific biochemical reactions mediated by isolated enzymes, immunosystems, tissues, organelles or whole cells to detect chemical compounds, usually by electrical, thermal or optical signals. (genomicglossaries.com)
  • The Raman-active reagents also have a wide range of applications, especially in clinical fields (e.g., immunoassays, imaging, and drug screening). (patsnap.com)
  • Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the Avidin-biotin interaction to be used for the development of robust and highly sensitive assays. (genomicglossaries.com)
  • This feature along with short assay runtime makes this method a useful platform for the development of high throughput assays to determine modular domain-ligand interactions which could have wide-ranging applications in both basic and translational cancer research. (biomedcentral.com)
  • Salicylate testing is a category of drug testing that is focused on detecting salicylates such as acetysalicylic acid for either biochemical or medical purposes. (wikipedia.org)
  • Analysis and comparability of the analytical performances of two RIA kits for the assay of atrial natriuretic peptides (ANP). (diastrip.com)
  • Before an EE run, the original fluorescence image of the target gel was captured using a CCD camera. (bvsalud.org)
  • After the 30-min EE (50 V, 6 mA, pH 7.4 PBS), the fluorescence image was also recorded by the CCD camera. (bvsalud.org)
  • We have developed PLA-SH2, an alternative in-solution modular domain binding assay that takes advantage of Proximity Ligation Assay and real-time PCR. (biomedcentral.com)
  • The introduction of a salicylate specific antigen labeled with fluorescein into the sample will mark the sample. (wikipedia.org)
  • In an oligonucleotide-tagged multiplex (OTM) assay, a mixture of SH2 domains with domain-specific DNA tags are incubated with a sample spot allowing for competitive binding (single sample per well) [ 8 ]. (biomedcentral.com)