d ) Validation of the yeast mRNA interactome using western blotting of input samples and eluate after interactome capture with specific antibodies (ADH1, alcohol dehydrogenase 1, PUB1) or against TAP-tagged proteins (PGK1, phosphoglycerate kinase 1, TDH1, triose phosphate dehydrogenase, TRX2, thioredoxine 2, SHE2, Swi5p-dependent HO Expression 2). (nature.com)
Recent studies have shown that cyclin-dependent kinase (CDK) inhibitors can have a tremendous impact on cell cycle progression in plants. (uni-bielefeld.de)
Based on reproducible and robust expression data, the genes Phosphoglycerate mutase, Germin 2 and Cysteine-rich Receptor-like Kinase 15 (CRK15) were chosen as marker genes for the gene set regulated by the JA-Ile-independent COI1-function. (uni-goettingen.de)
Functional analysis of the promoter of the 3-phosphoglycerate kinase gene of Aspergillus nidulans. (le.ac.uk)
The pgk- mutant strain that has been isolated is deficient in 3-phosphoglycerate kinase activity, requires both acetate and glycerol for growth, conidiates poorly, and is poisoned by even moderate concentrations of hexoses. (le.ac.uk)
DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a member of the phosphatidylinositol 3-kinase related kinase family is a well-known player in repairing DNA double strand break through non-homologous end joining pathway. (molcells.org)
DNA-dependent protein kinase catalytic subunit (DNA-PKcs), encoded by PRKDC gene, is a member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, which also includes the two DNA damage repair proteins, ataxia telangiectasia mutated (ATM) and ATM and Rad3-related protein (ATR). (molcells.org)
Recent studies have shown that the main enzyme defect in pyridoxine-dependent epilepsy is caused by alpha-aminoadipic acid semialdehyde dehydrogenase in the pathway of cerebral lysine degradation. (jmedcasereportsimages.org)
Phosphoglycerate dehydrogenase (PHGDH) is an essential enzyme catalyzing the synthesis of amino acid serine. (cancer-research-network.com)
In particular, BI-4924 is a lipophilic, highly plasma protein bound selective phosphoglycerate dehydrogenase (PHGDH) inhibitor (IC 50 =3 nM) with excellent microsomal, as well as hepatocytic stability. (cancer-research-network.com)
Phosphoglycerate mutase (PGM) is any enzyme that catalyzes step 8 of glycolysis - the internal transfer of a phosphate group from C-3 to C-2 which results in the conversion of 3-phosphoglycerate (3PG) to 2-phosphoglycerate (2PG) through a 2,3-bisphosphoglycerate intermediate. (wikipedia.org)
There are a total of three reactions dPGM can catalyze: a mutase reaction resulting in the conversion of 3PG to 2PG and vice versa, a phosphatase reaction creating phosphoglycerate from 2,3-bisphosphoglycerate, and a synthase reaction producing 2,3-bisphosphoglycerate from 1,3-bisphosphoglycerate similar to the enzyme bisphosphoglycerate mutase[citation needed]. (wikipedia.org)
3PG + P-Enzyme → 2,3BPG + Enzyme → 2PG + P-Enzyme 3-phosphoglycerate intermediate 2-phosphoglycerate ΔG°′=+1.1kcal/mol 3PG 2,3BPG 2PG Phosphoglycerate mutase exists primarily as a dimer of two either identical or closely related subunits of about 32kDa. (wikipedia.org)
Phosphoglycerate mutase has a small positive Gibbs free energy and this reaction proceeds easily in both directions. (wikipedia.org)
Interestingly, JA-signalling components such as NINJA and MYC2,3,4 did not influence the repressive function of COI1 on the expression of the Phosphoglycerate mutase. (uni-goettingen.de)
Protein2
No conclusion could be drawn concerning the involvement of JAZ-proteins, as a described dominant-negative effect of the JAZ1Δ3-protein did not even interfere with all JA-dependent processes. (uni-goettingen.de)
Binding-protein-dependent transport system inner membrane component [Interproscan]. (ntu.edu.sg)
Enzyme3
PGM is an isomerase enzyme, effectively transferring a phosphate group (PO43−) from the C-3 carbon of 3-phosphoglycerate to the C-2 carbon forming 2-phosphoglycerate. (wikipedia.org)
When 3-phosphoglycerate enters the active site, the phosphohistidine complex is positioned as to facilitate transfer of phosphate from enzyme to substrate C-2 creating a 2,3-bisphosphoglycerate intermediate. (wikipedia.org)
Dephosphorylation of the enzyme histidine actuates a local allosteric change in enzyme configuration which now aligns the substrates 3-C phosphate group with enzyme active site histidine and facilitates phosphate transfer returning the enzyme to its initial phosphorylated state and releasing product 2-phosphoglycerate. (wikipedia.org)
Gene3
By whole transcriptome analyses, we characterized glucosedependent and CcpA-dependent gene regulation in Clostridium difficile. (studyres.com)
The importance of targeting these constructs to a specific gene locus has been demonstrated by the analysis of a large number of transformed strains which reveals that the expression of the lacZ reporter gene is dependent upon the site of integration of the vector into the genome, and that when targeted to the qutE locus, is directly proportional to its copy number. (le.ac.uk)
Using a neural cortical stem cell model, we find that the epigenetic changes arising as a consequence of alcohol exposure are heavily dependent on the gene under investigation, the dose of alcohol encountered, and that the signatures arising acutely differ significantly from those observed after a 4-day recovery period. (biomedcentral.com)
Glycolytic1
Overall, the present study indicated that decreased JMJD5 expression promoted cell proliferation and glycolytic metabolism in pancreatic cancer cells in a c-Myc-dependent manner. (morulaivf.com)
Phosphorylation2
In the cofactor-dependent enzyme's initial state, the active site contains a phosphohistidine complex formed by phosphorylation of a specific histidine residue. (wikipedia.org)
BACKGROUND AND AIMS: ATP-dependent phosphofructokinases (PFKs) catalyse phosphorylation of the carbon-1 position of fructose-6-phosphate, to form fructose-1,6-bisphosphate. (bvsalud.org)
Genes2
As expected JA-dependent functions such as fertility, JA-sensitive root growth and JA-inducible JAZ10 expression were not restored, but the function of the COI1-proteins to repress the expression of marker genes were maintained. (uni-goettingen.de)
Vitamin B6-dependent epilepsies are aheterogeneous group of autosomal recessive diseases that are caused by mutations of five different genes involved in vitamin B6 metabolism [1]. (jmedcasereportsimages.org)
Phosphate4
An important finding was that CO2 dependent O2 evolution required inorganic phosphate (Pi) with a ratio of O2 evolved per Pi added of 3 to 1. (nartsignaling.com)
Here, we report that PEPCK-M-dependent glycerol phosphate formation from noncarbohydrate precursors (glyceroneogenesis) occurs in starved lung cancer cells and supports de novo glycerophospholipid synthesis. (ox.ac.uk)
Using stable isotope-labeled glutamine and lactate, we show that PEPCK-M generates phosphoenolpyruvate and 3-phosphoglycerate, which are at least partially converted to glycerol phosphate and incorporated into glycerophospholipids (GPL) under glucose and serum starvation. (ox.ac.uk)
Code errors leading to a lack of pyridoxal-5'-phosphate manifest as B6-dependent epilepsy, including pyridoxamine-5-phosphate oxidase (PNPO) deficiency, which affects the synthesis and recycling of pyridoxal-5'-phosphate [2,3]. (jmedcasereportsimages.org)
Reaction5
Light reaction or photochemical reaction takes place in thylakoid membrane or granum and it is completely dependent upon the light. (webreus.net)
The reactions require reduced NADP, which comes from a light-dependent reaction. (webreus.net)
Light Reaction (Image Source: actforlibraries.com) The light reaction of light dependent reaction occurs in the chloroplast of the mesophyll cells of the leaves. (webreus.net)
But, in case of light dependent reaction or light reaction of photosynthesis, it is most influenced by presence or absence of light. (pavementworks.com)
00:26 Biology A) ADP, Pi, and NADP+ A basic quiz of the light-dependent reaction in photosynthesis. (pavementworks.com)
Proteins1
Under ABA treatment, the NR proteins are degraded via a 26S-proteasome dependent manner, while the transcriptional regulation is the main manner to rapidly reduce the NIA1 levels under nitrogen deficiency and NaCl stress conditions. (bvsalud.org)
Carbon1
The potential energy in the ATP and NADPH produced during the light dependent reactions of photosynthesis can only be stored for a fraction of a … Carbon dioxide, water and light are required for photosynthesis to occur. (pavementworks.com)
Enzymes1
These enzymes are categorized into the two distinct classes of either cofactor-dependent (dPGM) or cofactor-independent (iPGM). (wikipedia.org)
Among these unconventional activities, an iron-dependent form of non-apoptotic cell death, termed ferroptosis, attracts great interest. (nature.com)
Completely dependent1
For years, the obligate intracellular eubacteria chlamydiae, were speculated to be energy parasites, completely dependent on the host cell for high-energy intermediates. (umanitoba.ca)
Pathways1
Here, we review the role of ferroptosis in p53-mediated tumor suppression, with a focus on what cellular factors are critical for p53-dependent ferroptosis during tumor suppression and how p53 modulates both the canonical (GPX4-dependent) and the non-canonical (GPX4-independent) ferroptosis pathways. (nature.com)
Family1
42329 iron %28metal%29 dependent repressor%2C DtxR family CP001857 CDS Arcpr_0049 complement(42326. (go.jp)
Activity2
PLA2 activity was dose dependent (data not shown) and the MPD determined in this study was 5.0 μg (S.D. ± 2.83 μg) ( Table 1). (coxsignaling.com)
Confirming its role in enhancing c-MYC activity, we found that elevated OTUB1 correlates with inferior clinical outcomes in the c-MYC-dependent cancer multiple myeloma, and overexpression of OTUB1 accelerates the growth of myeloma cells. (morulaivf.com)
Function1
The question to which extent the signal trans-duction chain of the novel COI1-function differs from the known COI1-dependent signal transduction pathway was also addressed. (uni-goettingen.de)
Human1
Akhavan O, Ghaderi E, Akhavan A: Size-dependent genotoxicity of graphene nanoplatelets in human stem cells. (nartsignaling.com)
Products1
The products of the light-dependent reactions of photosynthesis are A. sugar and water. (pavementworks.com)
Forms1
Humans are totally dependent on other organisms for converting atmospheric nitrogen into forms available to the body. (medmuv.com)