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  • epidemiology
  • We conducted a series of retrospective and prospective studies to characterize the epidemiology of dengue and population mobility patterns in Nha Trang city, Vietnam, with a view to rational design of trials of community-level interventions. (ajtmh.org)
  • flaviviridae
  • ZIKV is an RNA virus (non-segmented positive-sense RNA genome) that belongs to the Flaviviridae family and Flavivirus genus. (springer.com)
  • vaccines
  • These results have been considered somewhat disappointing by many experts in the field, highlighting the strategic importance to keep on going studies on alternative dengue vaccines. (biomedcentral.com)
  • incidence
  • A 10-year time series of dengue case notifications showed pronounced interannual variability, as well as spatial heterogeneity in ward-level dengue incidence (median annual coefficient of variation k = 0.47). (ajtmh.org)
  • serum
  • The serum and cerebral spinal fluid specimens had been referred to the Centers for Disease Control and Prevention (CDC) for confirmatory testing. (ajtmh.org)
  • A serum sample was collected on 21 January and tested at the Analytic Diagnostic Centre in Willemstad, Curaçao, where the diagnosis was confirmed by polymerase-chain reaction (PCR) on 25 January. (who.int)
  • A serum sample was collected from the patient on 21 January and sent to the Caribbean Public Health Agency (CARPHA) for laboratory testing. (who.int)
  • Serum dilutions, neutralization reactions, and virus growth were performed in 96-well plates. (ajtmh.org)
  • All blood samples were drawn into serum separator tubes, allowed to clot at room temperature for 1-2 hours, then stored at 4°C. Sera were separated into aliquots within 24 hours and stored at −70°C until laboratory testing. (biomedcentral.com)
  • Neutralizing sera were diluted to 1:5, followed by ten-fold serial dilutions using phosphate buffer solution (PBS) pH 7.5 with 30% fetal bovine serum, mixed with virus (for a final starting dilution of 1:10), and incubated. (biomedcentral.com)
  • detection of antibodies
  • When to test in order to reliably predict the detection of antibodies is determined by: from the date of last potential exposure (return to Canada), add 14 days for incubation period, plus one week viremia plus one week for the appearance IgM, or test one month after the individual has returned from her trip . (rqhealth.ca)