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  • genome
  • RNA viruses encode the information required for their replication in both their primary sequences and higher-order structures formed when the RNA genome strand folds back on itself, but the extent of higher-order structure has remained unclear. (pnas.org)
  • We use a new high-throughput RNA structure probing technology to identify RNA regions with tertiary folds and discover that roughly one-third of the dengue virus RNA genome forms higher-order interactions, many in regions functionally important for replication. (pnas.org)
  • A novel therapeutic approach prevents dengue virus from reproducing in humans by targeting and silencing key regions of the dengue genome essential for viral replication. (innovations-report.com)
  • In the article "Inhibition of Dengue Virus 2 Replication by Artificial MicroRNAs Targeting the Conserved Regions," the authors describe how they constructed artificial short strands of nucleic acids called microRNAs that specifically target these conserved sites in the dengue virus genome. (innovations-report.com)
  • Previous studies have shown that when NS3 is mutated, the enzyme's role in genome replication is curtailed and the virus cannot make copies of its genome, demonstrating NS3's importance in the virus' life cycle. (ucsd.edu)
  • gene
  • Dengue virus induces novel changes in gene expression of human umbilical vein endothelial cells," Journal of Virology , vol. 77, no. 21, pp. 11822-11832, 2004. (hindawi.com)
  • Garcia-Blanco and colleagues in Duke's RNAi facility were able to knock down gene function in fruit fly cells infected with a strain of the dengue virus known as DENV-2. (thaindian.com)
  • vaccine
  • The findings of the La Jolla Institute for Allergy and Immunology have major implications for efforts to develop a first-ever vaccine against the dangerous infectious dengue disorder. (medindia.net)
  • Scientists had long complained that the lack of a good animal model hampered efforts to develop a first-ever dengue vaccine. (medindia.net)
  • Dr. Shresta said her group's ADE findings emphasize the importance of special caution in designing a dengue virus vaccine. (medindia.net)
  • She said: "Researchers will have to be extremely careful to ensure that, under no conditions, will a dengue vaccine generate these subneutralizing antibody conditions. (medindia.net)
  • Dr. Halstead agreed and said: "The vaccine should cause you to make antibodies to each of the four dengue viruses. (medindia.net)
  • Despite the threat posed by the disease, developing a vaccine against dengue has so far proved challenging, according to Ram Sasisekharan, the Alfred H. Caspary Professor of Biological Engineering at MIT. (innovations-report.com)
  • There have been indications that vaccination against dengue can worsen the symptoms of the virus if the vaccine is given to people who have not had the virus previously," said Brian Geiss, associate professor of microbiology at Colorado State and co-author of the study. (ucsd.edu)
  • inhibit
  • By studying a group of cell lines from recovered dengue-infected patients over two years, the team identified the antibody, could attach itself strongly to a specific part of the dengue virus and inhibit it from attacking other cells. (thaindian.com)
  • For viruses to productively infect their hosts, they must evade or inhibit important elements of the innate immune system, namely the type I interferon (IFN) response, which negatively influences the subsequent development of antigen-specific adaptive immunity against those viruses. (mdpi.com)
  • Dengue virus (DENV) can inhibit both type I IFN production and signaling in susceptible human cells, including dendritic cells (DCs). (mdpi.com)
  • The compounds do not inhibit the closely related Kunjin virus. (harvard.edu)
  • Journal of Infectious Diseases
  • Early immune activation in acute dengue illness is related to development of plasma leakage and disease severity," Journal of Infectious Diseases , vol. 179, no. 4, pp. 755-762, 1999. (hindawi.com)
  • Early clinical and laboratory indicators of acute dengue illness," Journal of Infectious Diseases , vol. 176, no. 2, pp. 313-321, 1997. (hindawi.com)
  • World Health Organ
  • The World Health Organization estimates there may be 50-100 million dengue infections worldwide every year. (thaindian.com)
  • WHO/TDR, Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control -- New Edition , World Health Organization (WHO) and the Special Programme for Research and Training in Tropical Diseases (TDR), Geneva (Switzerland), 2009, http://apps.who.int/iris/bitstream/10665/44188/1/9789241547871_eng.pdf . (hindawi.com)
  • Halstead
  • Dr. Halstead said he got his first inkling of the phenomenon while doing extensive clinical studies of dengue virus patients in Thailand in the 1960s. (medindia.net)
  • S. B. Halstead, "Antibodies determine virulence in Dengue," Annals of the New York Academy of Sciences , vol. 1171, no. 1, pp. (hindawi.com)
  • S. B. Halstead and N. J. Marchette, "Biologic properties of dengue viruses following serial passage in primary dog kidney cells: studies at the University of Hawaii," The American Journal of Tropical Medicine and Hygiene , vol. 69, Supplement, no. 6, pp. 5-11, 2003. (hindawi.com)
  • strains
  • They found that both strains seemed to block the transmission of dengue virus, and that the wMel strain was able to infect almost the entire test population in just a few generations. (innovations-report.com)
  • Research Consortium
  • According to a study by the International Research Consortium on Dengue Risk Assessment, Management and Surveillance, up to 390 million people are infected with the dengue virus each year. (innovations-report.com)
  • North Carolina State University mathematical biologist Dr. Alun Lloyd is part of the Eliminate Dengue program, a research consortium that includes scientists from Australia and the United States. (innovations-report.com)
  • structural
  • We have discovered that an astonishing structural change occurs between the immature and mature dengue shells," said Kuhn, also professor of biology. (purdue.edu)
  • pathogenesis
  • In this review, we will evaluate the contribution of innate immunity evasion by DENV to the pathogenesis and host tropism of this virus. (mdpi.com)
  • targets
  • These highly conserved regions are ideal targets for antiviral drug development as they are unlikely to mutate and allow the virus to develop drug resistance. (innovations-report.com)
  • diseases
  • Dengue virus activates membrane TRAIL relocalization and IFN-alpha production by human plasmacytoid dendritic cells in vitro and in vivo," PLoS Neglected Tropical Diseases , vol. 7, no. 6, article e2257, 2013. (hindawi.com)
  • Department of Virus Diseases, Walter Reed Army Institute of Research, Washington 20307-5100. (asm.org)
  • Researchers
  • In a paper published today in the Proceedings of the National Academy of Sciences, the researchers, from MIT's Koch Institute of Integrative Cancer Research, present a novel approach to developing a dengue therapy using mutated antibodies. (innovations-report.com)
  • The researchers chose as their model an antibody known as 4E11, which has been shown in tests to neutralize dengue 1, 2 and 3, but not dengue 4. (innovations-report.com)
  • This significantly narrowed the number of possible changes, or mutations, the researchers needed to make antibody 4E11 in order to improve its ability to neutralize all four viruses, in particular dengue 4. (innovations-report.com)
  • Lloyd contributed mathematical models that helped the researchers interpret the results of their experiments, which pointed to the wMel strain as the bacterium with the greater potential for suppressing the spread of dengue virus. (innovations-report.com)
  • Three decades ago researchers identified NS3 as crucial to the virus' survival. (ucsd.edu)
  • disease
  • The origin of the name Dengue is unknown, but is thought to derive from the Swahili "ka-dinga pepo" (or "disease of the devil") corrupted to the Spanish word "dengue" which described the "fastidious" gait exhibited by sufferers as they walk. (giantmicrobes.com)
  • Evidence of vascular damage in dengue disease: demonstration of high levels of soluble cell adhesion molecules and circulating endothelial cells," Endothelium , vol. 13, no. 5, pp. 335-340, 2006. (hindawi.com)
  • Dengue is a nasty disease, and right now, there is no treatment for it and no way to prevent it," said Mariano Garcia-Blanco, professor of molecular genetics and microbiology at Duke University Medical Centre (DUMC) and study co-author. (thaindian.com)
  • peptides
  • We found stem-derived peptides from dengue virus (DV) bind stem-less E trimer and mimic the stem-reconfiguration step in the fusion pathway. (harvard.edu)
  • If you compare a virus to a pirate ship, these peptides are the grappling hooks by which they attach themselves to their prey," Kuhn said. (purdue.edu)
  • Fusion peptides allow the virus to prepare for boarding, so to speak. (purdue.edu)
  • The peptides need to be protected until the virus is ready to bond with a cell, so in the immature particle, each peptide is covered with a special cap that protects it until the time is right. (purdue.edu)
  • It is in examining the changes a virus undergoes for example, in the case of dengue, how it uncaps its fusion peptides to become an infectious agent that the team hopes to find clues to stopping the developmental process in its tracks. (purdue.edu)
  • particle
  • The pair solved the structure of the mature dengue virus particle last year (see related story), and Rossmann said the new findings were a significant step toward unraveling the behavior of viruses. (purdue.edu)
  • The research group used an advanced imaging technique, known as cryoelectron microscopy, to take 3-D pictures of the dengue particle the term experts use to denote a single virus. (purdue.edu)
  • Compared to the mature dengue particle, for example, the immature form is 15 percent greater in diameter. (purdue.edu)
  • The metamorphosis the dengue particle undergoes is so radical, he said, that there is a possibility the immature form the team has seen is not actually a step in dengue's development. (purdue.edu)