• Dengue serotype 3 viruses were isolated from patients in Brazil from 2002 through 2004. (cdc.gov)
  • Flavivirus 5' UTR are untranslated regions in the genome of viruses in the genus Flavivirus. (wikipedia.org)
  • Zika virus is related to dengue, yellow fever, Japanese encephalitis, and West Nile viruses. (getjar.com)
  • Attenuation of dengue (and other RNA viruses) with codon pair recoding can be explained by increased CpG/UpA dinucleotide frequencies. (ox.ac.uk)
  • One way wily viruses can get a leg up is by subverting the micro-RNA (miRNA) of their host. (feedstuffs.com)
  • To see if RNA viruses other than hepatitis C use host miRNA, the researchers developed a screening method that chemically attaches those miRNAs to their targets. (feedstuffs.com)
  • To find out, he and his colleagues used CLEAR-CLIP to analyze the interactions of 15 RNA viruses with their host miRNA. (feedstuffs.com)
  • Among them were several viruses known to cause human disease, including poliovirus, dengue and chikungunya virus. (feedstuffs.com)
  • 3 In addition, Flaviviridae such as Zika, Dengue and West Nile viruses, and Togaviridae such as Chikungunya also cause intraocular inflammation. (cehjournal.org)
  • Pathogen blocking has primarily been proposed as a tool to control dengue virus (DENV) transmission, however it works against a range of viruses, including Zika virus (ZIKV). (bvsalud.org)
  • Replication of all positive-stranded RNA viruses occurs in close association with virus-induced intracellular membrane structures. (denguevirusnet.com)
  • The dengue viruses belongs to the genus Flavivirus in the family Flaviviridae . (medchemexpress.com)
  • We extended these observations to two other flaviviruses, Dengue and Zika viruses. (nature.com)
  • The flavivirus family is comprised of RNA viruses- how about DNA viruses? (nature.com)
  • But for these types of viruses, RNA structures in the genome can influence their ability to function as much as encoded proteins. (phys.org)
  • RNA can form helices too, but in viruses such as SARS-CoV-2 and its relatives, it does so when a single molecule folds back on itself. (phys.org)
  • Research on other viruses has teased out how they use RNA structures to do their dirty work. (phys.org)
  • The Kuhn laboratory studies the replication of human RNA enveloped viruses that are known as alphaviruses and flaviviruses. (purdue.edu)
  • ZIKA , Dengue, Chikungunya & West Nile viruses - transmitted by the "Tiger" Mosquito - has become the biggest new viral challenge across the Globe in recent years. (diapro.it)
  • Such is the case with a wide range of LAMP-based research on pathogen detection, including many viruses, such as influenza , dengue , Chikungunya , and Zika . (thermofisher.com)
  • In contrast to most bacteria, fungi, or parasites, viruses are completely dependent on the host cell for their replication, hijacking the cell's biochemical machinery through the actions of viral genome-encoded factors. (genetex.com)
  • Included in this viral pathogen list are the influenza viruses, dengue virus, Japanese encephalitis virus (JEV), hepatitis viruses (e.g. (genetex.com)
  • The single-stranded (+)-RNA viruses comprise the largest group of viruses, which includes pathogens such as Dengue virus, Corona virus and West Nile virus. (fortunejournals.com)
  • Studies of the mechanism of Qβ infection can increase our general understanding of the single-stranded (+)-RNA viruses, which can be exploited in the pursuit to treat and prevent diseases caused by pathogenic (+)-RNA viruses. (fortunejournals.com)
  • Viruses have been divided into seven groups in the Baltimore classification, which is based on the nature of their genome and the replication hereof [2]. (fortunejournals.com)
  • The largest group is the single-stranded, positive sense (+)-RNA viruses, which comprises more than a third of all viruses. (fortunejournals.com)
  • Common for these viruses is that their genomes resemble mRNA and act directly as a template for translation upon infection. (fortunejournals.com)
  • The group of (+)-RNA viruses includes a number of pathogenic viruses such as Polio virus, Hepatitis A and C, Dengue virus and Severe Acute Respiratory Syndrome coronavirus [3]. (fortunejournals.com)
  • The (+)-RNA viruses have been used in the development of vaccines, as imaging tools and in virus inactivation studies [4, 5]. (fortunejournals.com)
  • With sequencing technologies becoming cheaper and easier to use, more groups are able to obtain whole genome sequences of viruses of public health and scientific importance. (biomedcentral.com)
  • The program can handle individual or batch submissions of different viruses to GenBank and correctly annotates multiple viruses, including those that contain ribosomal slippage or RNA editing without prior knowledge of the virus to be annotated. (biomedcentral.com)
  • The incredible diversity of DNA and RNA viruses presents a challenge for development of a universal annotator [ 17 ]. (biomedcentral.com)
  • Dengue viruses are positive-sense RNA viruses belonging to the genus. (antiviralbiologic.com)
  • Based on the success of this vaccine candidate, a strategy for the development of additional vaccine candidates representing the other three DEN virus serotypes was foreseen in which wild-type (wt) dengue viruses could be similarly attenuated for vaccine use by incorporation of mutations in the 3 UTR. (antiviralbiologic.com)
  • Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. (utmb.edu)
  • Firstly, we would like to thank you for the segment on the current debate as to the mechanism of how viruses can become attenuated through wholescale genome modification. (microbe.tv)
  • With regards to the Dengue paper under discussion, (i) the results in BHK cells and (ii) the effects of the Jak inhibitor 1 upon the attenuated phenotype of both the hmin and NS3hmin viruses (in accord with the C16 Atkinson data), suggest attenuation occurs through an unknown component of a cellular stress response pathway - and does not support the hypothesis of attenuation arising from a defect in translation. (microbe.tv)
  • ADE has been observed in case of a variety of viruses, most notably flaviviruses (e.g., dengue virus), HIV, and Ebola viruses. (tigs.res.in)
  • Fast evolution is more common in viruses containing RNA as their genome than those containing DNA. (tigs.res.in)
  • The Flaviviridae is a family of small enveloped viruses with RNA genomes of 9000-13 000 bases. (ox.ac.uk)
  • We report crystal structures for full-length, glycosylated NS1 from West Nile and dengue viruses. (nih.gov)
  • The ongoing COVID19 pandemic has made it abundantly clear that infectious diseases due to pathogenic viruses with RNA genomes represents a global public health problem. (council.science)
  • Mortality and morbidity due to known RNA viruses are high and the problem is compounded due to the appearance of new viruses due to animal-human conflicts. (council.science)
  • RNA viruses such as SARS-CoV-2, Influenza, Japanese Encephalitis Virus, Dengue virus, Chikungunya virus and HIV may cause death or result in long-term sequelae in recovered patients. (council.science)
  • To encourage a productive discussion and to disseminate knowledge about new advances in this area, we have organized, with support from the IUBMB, a focused meeting on the Biochemistry & Molecular Biology of RNA viruses. (council.science)
  • That year, 95% of dengue viruses isolated from patients were serotype 1 viruses belonging to two lineages that had diverged from an earlier, now extinct, lineage sometime before 1998. (cdc.gov)
  • The large number of clinical cases in 2001 may have been due, in part, to a preponderance of infections with dengue 1 viruses. (cdc.gov)
  • Phylogenetic studies have shown regular extinction of strains of dengue viruses in single locations and emergence of new strains ( 16 , 17 ), and it has been suggested that the appearance of more fit or more pathogenic viruses may occur as a result of immunologic selection during periods of intense transmission during outbreaks ( 18 ). (cdc.gov)
  • While weak selective pressure on the envelope (E) protein gene of some dengue viruses is evident ( 19 ), the most extensive changes in virus genotypes appear to be due to recombination or to possible genetic bottlenecks ( 16 , 17 , 20 ). (cdc.gov)
  • Some viruses have an outer envelope consisting of protein and lipid, surrounding a protein capsid complex with genomic RNA or DNA and sometimes enzymes needed for the first steps of viral replication. (msdmanuals.com)
  • Classification of viruses is principally according to their genome sequence taking into consideration nature and structure of their genome and their method of replication, but not according to the diseases they cause (see International Committee on Taxonomy of Viruses (ICTV), 2021 release ). (msdmanuals.com)
  • either DNA or RNA viruses may have single or double strands of genetic material. (msdmanuals.com)
  • Single-strand RNA viruses are further divided into those with (+) sense and (-) sense RNA. (msdmanuals.com)
  • Positive-sense RNA viruses possess a single-stranded RNA genome that can serve as messenger RNA (mRNA) that can be directly translated to produce an amino acid sequence. (msdmanuals.com)
  • Negative-sense RNA viruses possess a single-stranded negative-sense genome that first must synthesize a complementary positive-sense antigenome, which is then used to make genomic negative-sense RNA. (msdmanuals.com)
  • DNA viruses typically replicate in the host cell nucleus, and RNA viruses typically replicate in the cytoplasm. (msdmanuals.com)
  • Certain single-strand, (+) sense RNA viruses termed retroviruses use a very different method of replication. (msdmanuals.com)
  • Because RNA transcription does not involve the same error-checking mechanisms as DNA transcription, RNA viruses, particularly retroviruses, are particularly prone to mutation. (msdmanuals.com)
  • the genome of RNA viruses ranges from 3.5 kilobases (some retroviruses) to 27 kilobases (some reoviruses), and the genome of DNA viruses ranges from 5 kilobases (some parvoviruses) to 280 kilobases (some poxviruses). (msdmanuals.com)
  • Zika virus is a member of the Flaviviridae family and the genus Flavivirus (which includes yellow fever virus, West Nile virus, Japanese encephalitis virus, and dengue virus). (cdc.gov)
  • The genus includes human pathogens like Zika virus, West-Nile virus, Dengue virus, Yellow Fever virus and other. (wikipedia.org)
  • Zika virus is a member of the flavivirus family, which also includes yellow fever virus, dengue virus, Japanese encephalitis virus, and West Nile virus. (virology.ws)
  • Zika virus has a nonsegmented, single-stranded, positive-sense RNA genome. (getjar.com)
  • Researchers attempting to recover the entire Zika virus genome from an aborted fetus with microcephaly also observed virus particles in the brain. (asianscientist.com)
  • The medically important flaviviruses are Zika virus, dengue virus, Japanese encephalitis virus and West Nile virus. (asianscientist.com)
  • That Zika virus is more thermally stable than dengue virus structure-wise, and it is consistent with its ability to stay infectious at extreme, feverish temperatures. (asianscientist.com)
  • Zika virus behaves very similarly to dengue, so it is actually easier task to us to solve the structures compared to other groups who are new to this field. (asianscientist.com)
  • Zika virus and dengue virus are co-circulating flaviviruses with a widespread endemic range. (bvsalud.org)
  • Like other flaviviruses, Zika virus is enveloped and icosahedral and has a nonsegmented, single-stranded, positive-sense RNA genome. (diapro.it)
  • In 2015, Zika virus RNA was detected in the amniotic fluid of two pregnant women whose fetuses had microcephaly, indicating that the virus had crossed the placenta and could have caused a mother-to-child infection. (diapro.it)
  • ZIKA Virus - a flavivirus that is often misdiagnosed as Dengue or Chikungunya. (diapro.it)
  • It is a single-stranded positive-sense ribonucleic acid(RNA)virus, has its origin traced to Zika forest in Uganda. (bvsalud.org)
  • The Flavivirus positive-oriented, single-stranded RNA genome has a length of 10,000 - 11,000 bases. (wikipedia.org)
  • Flavivirus is a virus that contains a single strand RNA genome, which is surrounded by a lipid membrane. (asianscientist.com)
  • Dengue is caused by Dengue virus (DENV), a mosquito-borne flavivirus. (denguevirusnet.com)
  • DENV is an single stranded RNA positive-strand virus of the family Flaviviridae, genus Flavivirus. (denguevirusnet.com)
  • Dengue is a viral disease caused by four serotypes of the Flavivirus genus [ 1 ] and is prevalent in tropical and subtropical countries, ranging from Southeast Asia to the Americas [ 2 ]. (biomedcentral.com)
  • The database includes all publicly obtainable full-genome flavivirus sequences and access to series analysis equipment. (bioskinrevive.com)
  • The majority of known members in the genus Flavivirus are arthropod borne, and many are important human and veterinary pathogens (e.g. yellow fever virus, dengue virus). (ox.ac.uk)
  • The flavivirus nonstructural protein 1 (NS1) functions in genome replication as an intracellular dimer and in immune system evasion as a secreted hexamer. (nih.gov)
  • Dengue is a disease caused by four serotypes of a flavivirus of the same name ( 1 ). (cdc.gov)
  • The serotype of the virus was determined by performing indirect immunofluorescence ( 23 ) on cells from the cultures of C6-36 cells used for virus isolation with flavivirus-, dengue-, or serotype-specific monoclonal antibodies ( 23 , 24 ). (cdc.gov)
  • Collectively, the mutagenesis results demonstrate that NS2A functions in both DENV RNA synthesis and virion assembly/maturation. (rcsb.org)
  • The DENV RNA genome is in the infected cell translated by the host ribosomes. (denguevirusnet.com)
  • Among 20 laboratories testing Module A, 17 (85%) correctly detected DENV RNA by reverse transcription polymerase chain reaction (RT-PCR), 18 (90%) correctly determined serotype and 19 (95%) correctly identified CHIKV by RT-PCR. (who.int)
  • Analysis of cell-associated DENV RNA by oligo(dT) primed 5' capture sc" by Mark A. Sanborn, Tao Li et al. (uri.edu)
  • Both positive- and negative-sense DENV RNA was detected in reactions containing either an oligo(dT) primer alone, or in reactions supplemented with a DENV-specific primer. (uri.edu)
  • The addition of a DENV-specific primer did not increase the total amount of DENV RNA captured or the fraction of cells identified as containing DENV RNA. (uri.edu)
  • These results demonstrate that oligo(dT) primed 5' capture scRNAseq can detect DENV RNA and quantify virus-infected cells in physiologically relevant conditions, and provides insight into viral sequence variability within infected cells. (uri.edu)
  • An alanine substitution at this site completely abrogated the connection and DENV RNA replication, and both were restored by pseudoreversions A69S and A137V. (informationalwebs.com)
  • In a few cases, DF can progress to life-threatening dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). (cdc.gov)
  • It can cause symptoms ranging from self-limiting dengue fever to sometimes-fatal dengue hemorrhagic fever [ 1 ]. (biomedcentral.com)
  • DENV causes a wide range of diseases in humans, from a self limited Dengue Fever (DF) to a life-threatening syndrome called Dengue Hemorrhagic Fever (DHF) or Dengue Shock Syndrome (DSS) . (denguevirusnet.com)
  • 2006 For instance a primary an infection with one stress of DV may predispose a person to Dengue Hemorrhagic Fever a far more serious disease if contaminated subsequently using a different DV stress. (bioskinrevive.com)
  • In 2001, Myanmar (Burma) had its largest outbreak of dengue-15,361 reported cases of dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS), including 192 deaths. (cdc.gov)
  • In 1998, a pandemic of dengue resulted in 1.2 million cases of dengue hemorrhagic fever (DHF) in 56 countries ( 3 ). (cdc.gov)
  • Flaviviruses, the human pathogens responsible for dengue fever, West Nile fever, tick-borne encephalitis, and yellow fever, are endemic in tropical and temperate parts of the world. (nih.gov)
  • Clinical presentation of the infection is nonspecific and may often be confused with symptoms of other flaviviral diseases (dengue, West Nile [WN], and chikungunya). (bvsalud.org)
  • Like other flaviviruses, ZIKV is enveloped and icosahedral and has an 11-kb positive single-stranded RNA genome. (encyclopedia.pub)
  • This positive-sense RNA genome can be directly translated into viral proteins. (getjar.com)
  • The major difference for humans lies in subtle differences in the surface proteins of the different dengue subtypes. (denguevirusnet.com)
  • The viral nonstructural proteins use a negative-sense intermediate to replicate the positive-sense RNA genome, which then associates with capsid protein and is packaged into individual virions. (denguevirusnet.com)
  • These RCs seem to contain viral proteins, viral RNA and host cell factors. (denguevirusnet.com)
  • The core of the virus is the nucleocapsid, a structure that is made of the viral genome along with C proteins. (medchemexpress.com)
  • The general wisdom is that if we just focus on the proteins encoded in the virus's genome, we'll understand how SARS-CoV-2 works," Pyle says. (phys.org)
  • Viral proteins are made that redirect cellular machinery to establish sites for viral RNA synthesis, and concomitant virus particle assembly. (purdue.edu)
  • The RNA genome genes encode for nonstructural proteins and structural proteins, whose most immunogenic ones are NS1 and ENV antigens. (diapro.it)
  • Availability http://carnot.utmb.edu/flavitrack Get in touch with ude.bmtu@niehcshc Supplementary details http://carnot.utmb.edu/flavitrack/B1S1.html 1 Launch Flaviviruses (FV) (+)-strand RNA infections in the same genus seeing that hepaci- and pestiviruses are in charge of many emerging individual encephalitic and hemorrhagic illnesses (Barrett and Higgs 2007 The ~10.5 kb genome encodes an individual polyprotein that's cleaved into 10 viral proteins (Fig. 1). (bioskinrevive.com)
  • We studied the course of infection by analysing the production of viral RNA, proteins and phage particles produced in the cell-free reactions. (fortunejournals.com)
  • The replication of the viral RNA was determined by RT-PCR, while the translation of the viral proteins was examined by radiolabelling, and the production of infectious phage particles was evaluated by double-layered plaque assays. (fortunejournals.com)
  • Specifically, the viral RNA was replicated, the viral proteins were translated, and infectious phage particles were produced in the cell-free infection systems. (fortunejournals.com)
  • Virus particles have a nucleic acid genome and may also carry a few viral proteins, which are enclosed in a capsid of viral proteins or an envelope comprised of a small part of the host cell membrane. (fortunejournals.com)
  • The approximately 11,000-base genome contains a single open reading frame encoding a polyprotein which is processed by proteases of both viral and cellular origin into three structural proteins (C, prM, and E) and at least seven nonstructural (NS) proteins. (antiviralbiologic.com)
  • for most persons, this infection is either asymptomatic or dengue fever (DF) develops. (cdc.gov)
  • 50-100 million cases of dengue fever brate hosts of dengue virus in nature cessed by multiplex polymerase chain and 500 000 of dengue haemorrhagic are humans and several species of Asian reaction (PCR) in which the presence fever (DHF) reported annually ( 4 ). (who.int)
  • Genetics is closely attributed to Dengue fever in his study. (research.com)
  • A dramatic increase in the number of dengue fever and hemorrhagic dengue cases had been reported in the American continent. (unb.br)
  • The Ltc 1 peptide showed a significantly inhibitory effect against the dengue protease NS2B-NS3pro at 37°C, a physiological human temperature, (IC 50 , 12.68 ± 3.2 μM), and greater inhibitory effect was observed at 40°C, a temperature similar to a high fever (IC 50 , 6.58 ± 4.1 μM). (biomedcentral.com)
  • Patients presenting at Kasih Ibu Hospital with fever and clinical symptoms of dengue were asked to participate in the study. (warmadewa.ac.id)
  • Of these, 115 (86.5%) manifested dengue fever (DF) and 18 (13.5%) dengue haemorrhagic fever (DHF). (warmadewa.ac.id)
  • Conclusion: Our study confirms that dengue is one of the causes of fever in travellers visiting Bali. (warmadewa.ac.id)
  • Motivation Properly annotated series data for flaviviruses which trigger diseases such as for example tick-borne encephalitis (TBE) dengue fever (DF) Western world Nile PHA-739358 (WN) and yellow fever (YF) can certainly help in the look of antiviral medications and vaccines to avoid their pass on. (bioskinrevive.com)
  • Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. (utmb.edu)
  • Dengue virus belonging to family Flaviviridae is the most prevalent arthropod transmitted virus in humans. (biomedcentral.com)
  • The development of animal models of dengue virus (DENV) infection and disease has been challenging, as epidemic DENV does not naturally infect non-human species. (mdpi.com)
  • Dengue virus infection has emerged as marker in clinical practice ( 11 ). (who.int)
  • Now, the same team - including researchers in the laboratories of Charles M. Rice, the Maurice R. & Corinne P. Greenberg professor and head of the Laboratory of Virology & Infectious Disease, and Robert B. Darnell, the Robert & Harriet Heilbrunn professor and head of the Laboratory of Molecular Neuro-oncology - has found evidence that another RNA virus - bovine viral diarrhea (BVD) virus, which infects cows and other livestock - also depends on miRNA for infection. (feedstuffs.com)
  • This releases the RNA into the cell and infection starts. (denguevirusnet.com)
  • Dengue is a viral infection caused by dengue virus (DENV), which has a significant impact on public health worldwide. (bvsalud.org)
  • To define microRNA (miRNA) involvement during arbovirus infection of Aedes aegypti, we mined deep sequencing libraries of Dengue type 2 (DENV2)-exposed mosquitoes. (unicamp.br)
  • Once scientists have identified RNA structures that carry out key tasks, for instance, it may be possible to devise ways to disrupt them-and interfere with infection. (phys.org)
  • It's kind of amazing, all the different things RNA structures can do in viral infection," he says. (phys.org)
  • However, a quirk of SARS-CoV-2 infection-its RNA becomes unusually abundant-helped the team create a snapshot of the RNA genome's full structure. (phys.org)
  • In Malaysia, the fatality rate of dengue infection is approximately 3.6% based on the total number of dengue infections. (biomedcentral.com)
  • In addition to the global health problem caused by dengue infection, it also has an economic burden. (biomedcentral.com)
  • The estimated cost of dengue infection is approximately US$ 950 million per year, which is higher than hepatitis B and Japanese encephalitis in Southeast Asia [ 5 ]. (biomedcentral.com)
  • Background: Dengue, an acute febrile illness caused by infection with dengue virus (DENV), is endemic in Bali, Indonesia. (warmadewa.ac.id)
  • Accurate diagnosis, preventive measures and continuous disease surveillance will be useful for better management of dengue infection in travellers. (warmadewa.ac.id)
  • In this study, we have analysed the production of infectious Qβ phage particles in three different cell-free infection systems upon addition of the Qβ genome as a template. (fortunejournals.com)
  • However, the size, heterogeneity, and temporal dynamics of the cell-associated viral reservoir during acute dengue virus (DENV) infection remains unclear. (uri.edu)
  • In this study, we analyzed cells infected in vitro with DENV and PBMC from an individual experiencing a natural DENV infection utilizing 5' capture single cell RNA sequencing (scRNAseq). (uri.edu)
  • Vaccine development for the prevention of dengue infection is challenging because there are 4 different serotypes of dengue and an individual can be infected consecutively with different serotypes. (pasteur-kh.org)
  • Clinical outcome after infection range from asymptomatic infection to life threatening dengue shock syndrome. (pasteur-kh.org)
  • During replication, the 5' UTR interacts with the 3' UTR of the genome to initiate synthesis of new viral replicates and viral protein translation. (wikipedia.org)
  • Further, SLA is the promoter for RNA synthesis and interacts with the viral protein NS5 during circularization of the viral genome. (wikipedia.org)
  • The replicated RNA strand is held within a nucleocapsid formed from 12-kDa protein blocks. (getjar.com)
  • Identification of a new dengue virus inhibitor that targets the viral NS4B protein and restricts genomic RNA replication. (bris.ac.uk)
  • In conclusion, we have identified a new DENV inhibitor, SDM25N, which restricts genomic RNA replication by - directly or indirectly - targeting the viral NS4B protein. (bris.ac.uk)
  • In this study docking analysis was performed with active site of dengue NS2B-NS3 protein with selected plant flavonoids. (biomedcentral.com)
  • We hereby suggest that plant flavonoids could be used as potent inhibitors of dengue NS2B-NS3 protein and can be used as antiviral agents against dengue virus. (biomedcentral.com)
  • The hepatitis C virus, for example, uses a complex configuration of RNA to trick cells into making viral protein, according to Jeffrey Kieft, an RNA structural biologist and virologist at the University of Colorado Anschutz Medical Campus, who was not involved with Pyle's team's work. (phys.org)
  • Module A contained serum samples spiked with cultured dengue virus (DENV) or chikungunya virus (CHIKV) for the detection of nucleic acid and DENV non-structural protein 1 (NS1) antigen. (who.int)
  • The fundamental structure of the viral particle includes the DNA or RNA genome within a protein coat, or capsid. (genetex.com)
  • We examine at a molecular level the mechanisms of virus replication, such as virus protein synthesis, genome replication, and viral particle formation and their interplay with cellular processes. (ucsf.edu)
  • 2 FEATURES The major features of Flavitrack are sequence retrieval BLAST assessment of a given RNA or protein sequence to the people in the database and PHA-739358 sequence alignment of user selected sequences. (bioskinrevive.com)
  • IMPORTANCE With no authorized therapy or vaccine against dengue computer virus illness, the viral nonstructural protein 4B (NS4B) represents a possible drug Torin 1 price target, because it is definitely indispensable for computer virus replication. (informationalwebs.com)
  • The 5' UTR of flaviviruses are highly structured, has a length of approximately 100 nucleotides and harbors two conserved RNA secondary structures which are vital for the viral life cycle. (wikipedia.org)
  • These flavonoids can directly work as anti-dengue drug or with little modifications in their structures. (biomedcentral.com)
  • Although there have been considerable advances in the study of dengue virus, no vaccines or anti-dengue drugs are currently available for humans. (biomedcentral.com)
  • Therefore, new approaches are necessary for the development of potent anti-dengue drugs. (biomedcentral.com)
  • Therefore, further investigation is necessary to develop the Ltc 1 peptide as a new anti-dengue therapeutic. (biomedcentral.com)
  • These facts accentuate the need for new approaches to develop potent anti-dengue drugs. (biomedcentral.com)
  • The capsid-coding hairpin region (cHP) actually lies in the ORF of the viral genome and is followed by the 5'CS (conserved sequence), which forms another long-range RNA-RNA interaction with the 3' UTR (3'CS). (wikipedia.org)
  • We determined the frequency of different serotypes of dengue virus to highlight its hyperendemicity in Rawalpindi, Pakistan. (who.int)
  • Given these observations, and the finite pool of hosts in most locations (humans and selected species of Aedes mosquitoes), perhaps it is surprising that greater competition between the four serologically related serotypes of dengue virus has not been observed, e.g., the complete exclusion of two or three serotypes from an ecologic niche. (cdc.gov)
  • Location of the city of Belo Horizonte and its boroughs Pampulha, Nordeste, and Noroeste, Minas Gerais State, where samples of dengue virus serotype 3 were collected during 2002-2004. (cdc.gov)
  • The ratio of DHF to DSS cases in 2001 was not significantly different from that in 2000, when 1,816 cases of DHF/DSS were reported and dengue 1 also was the most frequently isolated serotype. (cdc.gov)
  • ABSTRACT Dengue is one of the most important arthropod-borne viral diseases. (who.int)
  • abstract = "Dengue virus (DENV) is an important human arthropod-borne virus with a major impact on public health. (bris.ac.uk)
  • Overall, each model has its advantages and disadvantages and is differentially suited for studies of dengue pathogenesis and immunopathogenesis and/or pre-clinical testing of antiviral drugs and vaccines. (mdpi.com)
  • This suggests that the virus or host immune response may affect the protective function of endothelial barriers, ultimately being considered the most relevant event in severe and fatal dengue pathogenesis. (bvsalud.org)
  • In this work we describe the diversity found in full-length DENV-1 sequences and phylogenetic inference of dengue virus isolated in Americas. (unb.br)
  • We found three recombination events among all DENV examined sequences, one of the new full length genome described here was apparently recombinant. (unb.br)
  • Our analysis of full genome sequences samples of DENV-1 population geographically structured and with low-diversity in Latin American has yielded strong evidence of relatively pervasive recombination. (unb.br)
  • The sequencing of the human genome revealed that at least 1% of the human genome consists of endogenous retroviral sequences, representing past encounters with retroviruses during the course of human evolution. (msdmanuals.com)
  • We analyzed the C-prM and the envelope genes of DENV-3 isolates related to different clinical manifestations of dengue disease from Minas Gerais State, Brazil, from 2002 through 2004. (cdc.gov)
  • Contorting its RNA into three-dimensional shapes gives SARS-CoV-2 another set of tools with which to compensate for a limited number of genes. (phys.org)
  • INTRODUCTION Dengue trojan (DENV) can be an enveloped plus-strand RNA trojan owned by the genus from the family members luciferase (Rluc)-expressing reporter trojan (pFK-DVs-R2A), the subgenomic reporter replicon (pFK-sgDVs-R2A), as well as the hygromycin B-selectable subgenomic replicon (pFK-sgDVs-H2A) had been defined previously (29). (informationalwebs.com)
  • Currently, dengue is the most significant mosquito-borne viral disease that affects humans. (cdc.gov)
  • In contrast, certain immunodeficient mouse models infected with mouse-adapted DENV strains show signs of severe disease similar to the 'vascular-leak' syndrome seen in severe dengue in humans. (mdpi.com)
  • GenBank currently has automatic prokaryotic and eukaryotic genome annotation pipelines but has no viral annotation pipeline beyond influenza virus. (biomedcentral.com)
  • State and federal public health laboratories have built capacity such that they now sequence more than 6000 influenza virus genomes and more than 5000 enteropathogenic bacterial genomes each year [ 11 ]. (biomedcentral.com)
  • Surprisingly, other than for influenza virus, NCBI GenBank does not currently have an automatic viral genome annotation pipeline. (biomedcentral.com)
  • After entering the cell, the replication of the viral RNA genome is influenced by circadian regulated lipid genesis pathways. (nature.com)
  • Clinical and laboratory assessments were performed and sera were collected for molecular analysis, which included DENV serotyping, genome sequencing and phylogenetic analysis. (warmadewa.ac.id)
  • RNA extraction is essential for the molecular detection of common viral pathogens. (bvsalud.org)
  • Furthermore, the incidence of disease and its severity vary between primary and secondary infections and between infections with different dengue virus serotypes ( 6 - 8 ). (cdc.gov)
  • The virus has a genome of about 11000 bases that encodes a single large polyprotein that is subsequently cleaved into several structural and non-structural mature peptides. (denguevirusnet.com)
  • Despite having a very small DNA genome, the virus encodes multiple genetic elements that can bind circadian transcription factors. (nature.com)
  • DENV is an enveloped virus with a positive stranded RNA genome of approximately 11 kb in length that encodes a single polypeptide. (biomedcentral.com)
  • We also have a lot of experience with imaging dengue structures to high resolution. (asianscientist.com)
  • The novel coronavirus uses structures within its RNA to infect cells. (phys.org)
  • A team led by RNA scientist Anna Marie Pyle has now made the most comprehensive map to date of these genomic structures. (phys.org)
  • In two preprints posted in July 2020 to bioRxiv.org, Pyle's team mapped structures across the entire RNA genome of the coronavirus SARS-CoV-2, using living cells and computational analyses. (phys.org)
  • SARS-CoV-2 relies on its unique RNA structures to infect people and cause the illness COVID-19. (phys.org)
  • Previous efforts using HIV- and hepatitis C-infected cells did not produce enough information to create a full inventory of RNA structures. (phys.org)
  • The 3 UTR is nearly 400 bases in length and is predicted to contain several stem-loop structures conserved among dengue virus serotypes (3, 9, 14, 17). (antiviralbiologic.com)
  • Permuting codon usage in this manner would destroy any undetected RNA structures within these regions of the viral sequence. (microbe.tv)
  • Notably, 11.9% of dengue-like disease patients showed VEEV infections. (bvsalud.org)
  • suggested that there are 390 million dengue virus (DENV) infections per year, of which 96 million manifest clinically. (who.int)
  • Remarkably, an R84A mutation did not affect viral RNA synthesis but blocked intracellular formation of infectious virions. (rcsb.org)
  • Dengue is an acute systemic viral infectious disease, established worldwide in epidemic and endemic transmission cycles. (bvsalud.org)
  • d WHO Collaborating Centre for Arbovirus Reference and Research (Dengue/Severe Dengue), Tropical Infectious Diseases Research and Education Centre, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. (who.int)
  • Many foresee a world in which nearly every infectious disease genome is sequenced and archived in a publicly searchable database [ 9 , 10 ]. (biomedcentral.com)
  • A great deal of series and structural data is currently designed for FV such as for example WN and dengue (DV) that have pass on worldwide because of raising intercontinental travel rest in vector control and insufficient effective antiviral medications (Kuno and Chang 2005 Mackenzie et al . (bioskinrevive.com)
  • The strong correlation between NS3-NS4B connection and RNA replication provides strong evidence that this complicated performs a pivotal function in the viral replication routine, representing a appealing antiviral medicine focus on Torin 1 price hence. (informationalwebs.com)
  • The NS2B-NS3 protease contains two functional regions i.e., a C-terminal region acting as RNA helicase and a N-terminal 180-residue is a trypsin like serine protease (Fig. 1 ). (biomedcentral.com)
  • In this study, in-silico screening using automated docking method was performed and binding models of dengue NS2B-NS3 protease with selected plant flavonoids are proposed. (biomedcentral.com)
  • For instance, the non-homologous DNA end joining and homologous recombination pathways are central cellular mechanisms by which eukaryotic cells maintain genome integrity. (bvsalud.org)
  • Last year, Rockefeller scientists were surprised to discover that hepatitis C, a virus that contains RNA rather than DNA, not only sequesters a host miRNA but relies on it. (feedstuffs.com)
  • Using a transient subgenomic replicon, we demonstrate that SDM25N restricts genomic RNA replication rather than translation of the viral genome. (bris.ac.uk)
  • There is currently no specific treatment for dengue, and vector control and vaccination are the only effective methods to prevent future outbreaks. (who.int)
  • Methods: We performed a prospective cross-sectional dengue study involving foreign travellers visiting Bali, Indonesia in the period of 2015-17. (warmadewa.ac.id)
  • Clinical symptoms resemble dengue, and while chikungunya is a generally milder disease, debilitating sequelae such as persistent arthralgia have been reported in 36-64% of cases. (who.int)
  • MicroRNA levels are modulated in Aedes aegypti after exposure to Dengue-2. (unicamp.br)
  • Viral RNA is translated into a single polyprotein. (biomedcentral.com)
  • Furthermore, while the majority of intracellular DENV sequence captured in this analysis mapped to the 5' end of the viral genome, distinct patterns of enhanced coverage within the DENV polyprotein coding region were observed. (uri.edu)
  • Incidence of dengue has increased many fold in the past 50 years and has expanded to areas that were previously free from the disease. (who.int)
  • VEEV complex RNA was found in five suspected dengue cases from disease surveillance. (bvsalud.org)
  • BACKGROUND: Dengue is the most common vector-borne viral disease worldwide. (bvsalud.org)
  • Information on the epidemiology and virological aspects of dengue in these tourists is important to gain a better understanding of the dengue disease in international travellers. (warmadewa.ac.id)
  • Genome-wide transcriptomic studies suggest that ADE also suppresses the operation of anti-viral pathways in immune cells, thereby enhancing disease manifestations. (tigs.res.in)
  • This manageable size together with the current advances in nucleotide sequencing technology means that partial and whole virus genome sequencing will become an essential component in epidemiologic investigations of disease outbreaks. (msdmanuals.com)
  • Global dengue incidence has increased in recent decades, though the actual numbers of dengue cases are masked by underreporting. (who.int)
  • However, when R84 was replaced with E, the mutation attenuated both viral RNA synthesis and virus production. (rcsb.org)
  • One such stem-loop structure, identified as TL2 in the proposed secondary structure of the 3 UTR (14), was previously removed by deletion of 30 nucleotides from the DEN4 genome (3 nucleotides 172 to 143) (12) and has subsequently been designated as the 30 mutation (5). (antiviralbiologic.com)
  • The original pRS424DEN1WP cDNA clone was digested Phellodendrine chloride with strain STBL2 (Invitrogen, Carlsbad, Calif.). Plasmid DNA suitable for generating RNA transcripts was prepared, and the presence of the 30 mutation was verified by sequence analysis. (antiviralbiologic.com)
  • Biotechnological approaches targeted toward ZIKV vector control include the development of transgenic mosquitoes to disrupt the genome pool of wild strains and use of an endosymbiotic bacterium to prevent replication of arboviruses within its vector. (bvsalud.org)
  • or 3) dengue virus was recovered by culturing 140 μL of acute-phase serum on 25-cm 2 monolayers of C6-36 A. albopictus cells for 7 days. (cdc.gov)