• In this study, we investigated further the cellular expression and relative tissue levels of human CYP2D6 in these transgenic mice in liver, intestine, kidney, and brain. (aspetjournals.org)
  • Human CYP2D6 was not detected in brain of transgenic mice. (aspetjournals.org)
  • To address this question, we have generated transgenic mice expressing only the H chain gene or both H and L chain genes of the 6-19 IgG3 anti-IgG2a RF and determined the development of glomerular and vascular lesions. (unige.ch)
  • H-single and H/L-double transgenic mice displayed comparable high amounts of IgG3 cryoglobulins, but only H/L-double transgenic mice having 10-fold higher levels of IgG3 anti-IgG2a RF progressively developed chronic, lethal glomerulonephritis. (unige.ch)
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
  • [ 8 ] Despite higher TT, transgenic mice had hypogonadal features, including a weight reduction in seminal vesicles and of androgen-dependent pelvic floor muscles. (medscape.com)
  • identified a spontaneous mutation in C57BL/10ScSn inbred mice that exhibited a disease state similar to human DMD [ 4 ]. (hindawi.com)
  • C57BL/6J mice were immunized with elastin or elastin peptide oxidatively modified by cigarette smoke (ox-elastin). (utah.edu)
  • They used this model to study age-dependent impairment of interferon (IFN) immunity and COVID-19 aggravation in standard C57 black 6 (C57BL/6) wild-type (wt) mice. (news-medical.net)
  • In the present study, researchers analyzed the individual and combined role of type I and III IFNs in limiting SARS-CoV-2 replication in mice lacking functional type I (Ifnar1 -/- ) or type III IFN receptors (Ifnlr1 -/- ) and C57BL/6 wt mice infected with MA SARS-CoV-2. (news-medical.net)
  • The adult C57BL/6 mice initiated a rapid innate and adaptive immune response associated with high IFN-γ and low interleukin-10 (IL-10) expression. (news-medical.net)
  • The adult C57BL/6 mice lacking functional type I (Ifnar1 -/- ) or type III IFN receptors (Ifnlr1 -/- ) had about 10-fold increased viral loads in the lungs and upper airways. (news-medical.net)
  • In this study, the inbred mouse strains A/J and C57BL/6J were used to determine whether their specific strain differences correlate with differences in susceptibility to respiratory depression and constipation. (pacific.edu)
  • C57BL/6J mice showed a greater degree of change in several respiratory parameters, resulting in more pronounced respiratory depression. (pacific.edu)
  • This study demonstrates that the strain differences between A/J and C57BL/6J mice have a major effect on opioid-induced constipation and respiratory depression. (pacific.edu)
  • Here, we use an in vitro assay of protein synthesis in the hippocampus of male Fmr1 knock-out (KO) mice to explore the molecular mechanisms involved in this core biochemical phenotype under conditions where aberrant synaptic physiology has been observed. (jneurosci.org)
  • Binding specificity of ALCAM-MPIO was demonstrated in vitro under static and flow conditions. (ox.ac.uk)
  • To carry out these legislative mandates, ATSDR's Division of Toxicology (DT) has developed and coordinated a mixtures program that includes trend analysis to identify the mixtures most often found in environmental media, in vivo and in vitro toxicological testing of mixtures, quantitative modeling of joint action, and methodological development for assessment of joint toxicity. (cdc.gov)
  • The inhibitors demonstrated promising effects in vitro with reduced viability, increased differentiation and cell death of neuroblastoma cells. (lu.se)
  • In conclusion, the presented mouse models of colorectal cancer further strengthen our previous in vitro findings and highlight the prospect of CysLT1R as an alternative therapeutic approach. (lu.se)
  • There are genes modifying the cancer susceptibility of these mouse models. (wikipedia.org)
  • The original development and use of inbred mice for probing the genetic determinants of resistance and susceptibility to infections and tumors was documented from research by Clara J. Lynch (Morse 1978). (experts.com)
  • Here, we used the Df1 /+ mouse model of 22q11.2DS to investigate the relationship between hearing loss and susceptibility to schizophrenia-relevant brain and behavioral abnormalities. (biorxiv.org)
  • Df1 /+ mice have a multi-gene deletion analogous to the chromosomal microdeletion that causes human 22q11.2DS, and like human 22q11.2DS patients exhibit high rates of hearing loss arising primarily from susceptibility to middle ear inflammation. (biorxiv.org)
  • Mouse model demonstrates strain differences in susceptibility to opioi" by Andrew L. Young, Archana Viswanath et al. (pacific.edu)
  • Previous studies have demonstrated changes in the RNA expression in cyclically stressed osteoblasts obtained from mouse calvaria. (allenpress.com)
  • Relatively recent studies have demonstrated an association between childhood infection with H pylori and low serum iron and hypochlorhydria. (medscape.com)
  • Regardless of this justification, some studies have demonstrated negative action of L-PRP on osteogenesis 1,10,16,18 at the same time that fibrosis is produced 5,6 generating conflict about the real clinical effect of L-PRP in bone reparative sites. (bvsalud.org)
  • We establish the extent and structure of genotype-by-epigenotype interaction using embryonic stem cells derived from Diversity Outbred mice. (elifesciences.org)
  • Sustained APPsα overexpression in aged mice with already preexisting pathology and amyloidosis restored synaptic plasticity and partially rescued spine density deficits. (springer.com)
  • Conventional pertussis animal models deliver hundreds of thousands of Bordetella pertussis bacteria deep into the lungs, rapidly inducing severe pneumonic pathology and a robust immune response. (cdc.gov)
  • Here we assess the role of elastin antibodies using a mouse model of smoke-induced ocular pathology (SIOP), which similarly demonstrates EL loss. (utah.edu)
  • These data demonstrate that SIOP mice generate elastin-specific antibodies and that immunization with ox-elastin exacerbates ocular pathology. (utah.edu)
  • Moreover, PV+ inhibitory interneurons, another marker for schizophrenia pathology, were significantly reduced in density in auditory cortex but not secondary motor cortex of Df1 /+ mice with hearing loss. (biorxiv.org)
  • Previous work found that a lead p75 NTR ligand, LM11A-31, prevents degeneration of cholinergic neurites when given to an AD mouse model in the early stages of disease pathology. (plos.org)
  • To extend its potential clinical applications, we sought to determine whether LM11A-31 could reverse cholinergic neurite atrophy when treatment begins in AD mouse models having mid- to late stages of pathology. (plos.org)
  • In this study, LM11A-31 (50 or 75 mg/kg) was administered orally to two AD mouse models, Thy-1 hAPP Lond/Swe (APP L/S ) and Tg2576, at age ranges during which marked AD-like pathology manifests. (plos.org)
  • Importantly, a 1 month LM11A-31 treatment given to male APP L/S mice (12-13 months old) with late-stage pathology reversed the degeneration of cholinergic neurites in basal forebrain, ameliorated cortical dystrophic neurites, and normalized increased basal forebrain levels of p75 NTR . (plos.org)
  • We demonstrated histological evidence of persistent ectopic synapses, prolonged cellular apoptosis, and gliosis in the OIR retina that corresponded with long-term in vivo evidence of capillary attrition, inner retinal thinning, and dysfunction despite full peripheral revascularization. (molvis.org)
  • Finally, we demonstrate that exhausted T cells are reinvigorated towards CLL cytotoxicity by inhibition of PDCD1/CD274 interaction in vivo. (nih.gov)
  • Both in vivo near-infrared imaging of caspase activity and TUNEL staining demonstrated that TRA-8 rapidly induced apoptosis of macrophages in inflamed synovium. (nih.gov)
  • Collectively, our data indicate that Sarm1 ablation increases mitochondrial bioenergetics and confers histological and functional protection in vivo in the mouse retina against mitochondrial dysfunction, a hallmark of many neurodegenerative conditions including a variety of ocular disorders. (tcd.ie)
  • The primary dysmenorrhea test aimed to verify the effect of AqECsR on induced abdominal contortions in female mice in vivo and to perform a morphometric analysis of the organs. (bvsalud.org)
  • However, in the antidysmenorrheal activity test, AqECsR demonstrated a significant in vivo effect of reducing oxytocin -induced abdominal contortions. (bvsalud.org)
  • We pharmacologically inhibited PKCe in ex vivo mouse models of aging. (cdc.gov)
  • Moreover, The use of animals as surrogate rine host, can provide a platform for animal models for tumour viruses in hosts for the study of human tu- in vivo infection. (who.int)
  • For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)
  • In vivo, combination treatment resulted in slower tumor growth and increased survival of neuroblastoma PDX models. (lu.se)
  • KSP inhibition caused tumor regression in vivo and resulted in increased survival of multiple PDX models. (lu.se)
  • In vivo, rigosertib treatment resulted in decreased tumor growth and increased survival of PDX mice. (lu.se)
  • Methods and results We undertook a microarray approach to identify dysregulated miRNAs following engraftment in an interpositional porcine graft model. (ed.ac.uk)
  • Thus, this study aimed to identify microRNAs (miRNAs) that are dysregulated during neointimal formation and determine their pathophysiological relevance following miRNA manipulation.Methods and results We undertook a microarray approach to identify dysregulated miRNAs following engraftment in an interpositional porcine graft model. (ed.ac.uk)
  • Methods: We replaced DCs by peripheral mononuclear cells and myelin autoantigens by glatiramer acetate (Copaxone (R)), a drug approved for the treatment of MS. Spleen cells were loaded with Copaxone (R), incubated with mitomycin C (MICCop) and injected into mice after the first bout of relapsing-remitting EAE. (uni-koeln.de)
  • Here, we show that hESC-MSCs prevent the progression of fatal lupus nephritis (LN) in NZB/W F1 (BWF1) mice. (nature.com)
  • These models enable researchers to study the onset, progression of the disease, and understand in depth the molecular events that contribute to the development and spread of colorectal cancer. (wikipedia.org)
  • Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
  • We demonstrate the sensitivity of the last component of the Stokes vector, the degree of polarization and high-order statistical moments of depolarization to the structural alterations in brain tissue, which correspond to the disease progression. (spiedigitallibrary.org)
  • Mariia Borovkova , Alexander Bykov , Alexey Popov , Angelo Pierangelo , Tatiana Novikova , Jens Pahnke , and Igor Meglinski "The use of Stokes-Mueller polarimetry for assessment of amyloid-β progression in a mouse model of Alzheimer's disease", Proc. (spiedigitallibrary.org)
  • However, the mechanism of tumor progression in FAP/sporadic colorectal cancer and in colitis-associated colorectal cancer might differ as indicated by reduced tumor expression of COX-2 and reduced serum levels of PGE2 in the female double mutant (CysLT1R−/− ApcMin/+) mice, whereas AOM/DSS-treated female single mutant (CysLT1R−/−) mice demonstrated increased serum levels of PGE2. (lu.se)
  • We confirmed this finding with CTCF ChIP-seq that revealed strain-specific binding in the inbred founder mice. (elifesciences.org)
  • During the 1920's, she personally brought a mouse strain into the United States from a laboratory in Lausanne, Switzerland (Lynch 1969). (experts.com)
  • Sixty-four mice ( Mus musculus ), female, adult, Swiss strain, weighing approximately 20 g, were divided into four groups: G1: control (n = 24), G2: nephropathy group (N) (n = 7), G3, DM (n = 9), and G4: N+DM (n = 24). (scielo.br)
  • We have studied the transgenic mouse strain, Tyrp-1-TAg, to try to gain insight into possible molecular mechanisms common to pigmented ocular neoplasms occurring spontaneously in the eyes of these mice and human choroidal melanoma. (molvis.org)
  • In a recent study posted to the bioRxiv * pre-print server, researchers developed a highly pathogenic mouse-adapted (MA) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strain to model mild, moderate, or severe coronavirus disease 2019 (COVID-19). (news-medical.net)
  • Also, in a strain of mice that develop both emphysema and fibrosis after chronic cigarette-smoke exposure, the presence of elastase in alveolar structures is associated with a positive immunohistochemical reaction for reaction for both TGF-β and TGF-α. (biomedcentral.com)
  • Mice with mutations in transforming growth factor-β1 gene introduced into 129/Sv Rag2 mutant mouse accelerates adenocarcinomas with strong local invasion suggesting a role for genetic background in tumor development. (wikipedia.org)
  • In both genetic and diet-induced models of insulin resistance, CHOP deficiency improved β cell ultrastructure and promoted cell survival. (jci.org)
  • This mouse population segregates millions of variants from eight inbred founders, enabling precision genetic mapping with extensive genotypic and phenotypic diversity. (elifesciences.org)
  • With 176 samples profiled for genotype, gene expression, and open chromatin, we used regression modeling to infer genetic-epigenetic interactions on a genome-wide scale. (elifesciences.org)
  • Our results demonstrate that statistical interactions between genetic variants and chromatin accessibility are common throughout the genome. (elifesciences.org)
  • In two mitochondrial disease models, hypoxia corrects defects that arise as a consequence of the genetic lesion. (fightaging.org)
  • Unlike cancer arising in the human population, tumors in genetically engineered mouse models arise in mice with well defined genetic backgrounds where genetic variability can be minimized. (molvis.org)
  • Genetic ablation of miR-21 in mice or grafted veins dramatically reduced neointimal formation in a mouse model of vein grafting. (ed.ac.uk)
  • outer and inner retinal dysfunction were demonstrated with fERG. (molvis.org)
  • Mouse models of colorectal cancer and intestinal cancer are experimental systems in which mice are genetically manipulated, fed a modified diet, or challenged with chemicals to develop malignancies in the gastrointestinal tract. (wikipedia.org)
  • This experimental approach can measure aspects of the infection processes not observed in the conventional pneumonic infection model. (cdc.gov)
  • In the experimental autoimmune encephalitis model of multiple sclerosis , continuous 10% oxygen promotes vascular integrity and apoptosis of infiltrating leukocytes . (fightaging.org)
  • To create an experimental model of associated comorbidities, for healing and regeneration studies, protocols for induction of nephropathy by ischemia and reperfusion (I/R) and induction of DM by injection of streptozotocin (STZ) were associated. (scielo.br)
  • Oxygen restriction is an exciting experimental modality, and it's now been shown to have pretty potent beneficial effects across multiple models of aging and disease," said Robert Rogers, first author on the PLOS study, postdoctoral fellow in Mootha's lab, and an instructor in the Division of Pulmonary and Critical Care Medicine at Massachusetts General Hospital. (broadinstitute.org)
  • Experimental procedures of a mouse DMT model. (jci.org)
  • Mark Baxter, PhD , a neuroscientist working at the Icahn School of Medicine at Mount Sinai, began his presentation with a simple suggestion bearing deep implications for experimental design and interpretation: instead of trying to create animal models of postoperative delirium, we should develop models for postoperative delirium. (iars.org)
  • Background: Dendritic cells (DCs) rendered suppressive by treatment with mitomycin C and loaded with the autoantigen myelin basic protein demonstrated earlier their ability to prevent experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis (MS). This provides an approach for prophylactic vaccination against autoimmune diseases. (uni-koeln.de)
  • In this study we investigate in experimental animal models of emphysema and fibrosis whether neutrophil elastase may constitute a pathogenic link between these two pathologies. (biomedcentral.com)
  • 2003). These so-called nude mice have a marked ability to engraft many different types of tumor cells from other animals, including humans. (experts.com)
  • Although a close connection between uterine regeneration and successful pregnancy in both humans and mice has been consistently observed, its molecular basis remains unclear. (jci.org)
  • The cell-specific expression of human CYP2D6 in liver, kidney, and intestine in humanized mice was similar to that reported in humans. (aspetjournals.org)
  • The expression patterns of mouse CYP2D proteins were similar to those in humans in liver and kidney but substantially different in intestine. (aspetjournals.org)
  • A probiotic intervention was also demonstrated to mitigate the risk of testing positive in mice, paving the way for future studies in humans. (iars.org)
  • In contrast, certain immunodeficient mouse models infected with mouse-adapted DENV strains show signs of severe disease similar to the 'vascular-leak' syndrome seen in severe dengue in humans. (mdpi.com)
  • Indeed a wealth of studies from humans and mouse models establishes a particularly potent role for CD8 T cells in controlling the outgrowth of malignancies ( 3 ). (frontiersin.org)
  • One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
  • In this combined immunodeficiency (SCID) cancer is low in these species (as it chapter, some aspects of this issue mice, in which the human target is in humans), which renders cancer are discussed. (who.int)
  • that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
  • Lamivudine, a commonly-used antiretroviral drug for treating HIV, improves cognition in a mouse model of Down syndrome, according to the findings of a joint new study by researchers at the Centre for Genomic Regulation (CRG) and the IrsiCaixa AIDS Research Institute, a centre jointly promoted by the "la Caixa" Foundation and the Department of Health of the Generalitat de Catalunya. (scienceblog.com)
  • Our findings add to a nascent but burgeoning literature on the beneficial effect of hypoxia in a wide variety of neurologic disease models. (fightaging.org)
  • Previous work from his lab suggested that hypoxia could prevent ataxia - impaired balance and coordination - in Friedreich's ataxia mice, and the new findings now show that the approach can even reverse it in animals with more advanced disease. (broadinstitute.org)
  • These findings suggest that LM11A-31 can reduce and/or reverse fundamental AD pathologies in late-stage AD mice. (plos.org)
  • This point again highlights Dr. Xie's findings with the "CAM in mice. (iars.org)
  • Together these findings showed that age-dependent type I and type III IFN responses synergize to limit excessive SARS-CoV-2 replication, expedite virus clearance, and protection against symptomatic disease in aged mice. (news-medical.net)
  • With combination of Min and Mom1 mutations the lifespan of FAP mouse models of colorectal cancer is increased. (wikipedia.org)
  • In the presented papers in this thesis we investigated the role of CysLT1R in different mouse models of colorectal cancer. (lu.se)
  • We also investigated the role of CysLT1R in 1) FAP/sporadic colorectal cancer by crossing ApcMin/+ mice with mice lacking CysLT1R expression and in 2) colitis-associated colorectal cancer by employing the AOM/DSS-model on mice lacking CysLT1R expression. (lu.se)
  • Importantly, the regenerating epithelium around the DUM demonstrated heightened STAT3 phosphorylation and cell proliferation, which was suppressed in uteri of Stat3 conditional knockout mice. (jci.org)
  • Sarm1 knockout mice retain visual function after rotenone insult, displaying preservation of photopic negative response following rotenone treatment in addition to significantly higher optokinetic response measurements than wild type mice following rotenone. (tcd.ie)
  • A ) A schematic illustration of a mouse model of decellularized matrix transplantation (DMT). (jci.org)
  • Schematic diagram demonstrating the proposed potential mechanisms involving in neuroprotective effects of loganin. (aging-us.com)
  • In these models, high doses of B. pertussis, often 10 5 -10 6 CFU, are delivered to the lungs of rodents ( 20 , 21 ). (cdc.gov)
  • however, a new model using high doses of DENV has recently been shown to develop hemorrhagic signs after infection. (mdpi.com)
  • C57BL6J mice also showed significantly more constipation than A/J mice with 40 and 60 mg/kg morphine doses. (pacific.edu)
  • PLP-α-syn mice have been shown to recapitulate several features of MSA such as motor dysfunction/parkinsonism ( 17 , 18 ), respiratory dysfunction ( 20 ) and dysautonomia ( 21 ). (frontiersin.org)
  • In this study, to obtain a better understanding of the potential availability of A. trifoliatus for the treatment of cognitive and emotional dysfunction, we elucidated the antidementia and antidepressive effects of A. trifoliatus using an animal model of olfaction deficits. (hindawi.com)
  • As is always the case, recall that when we say "accelerated aging" what we really mean is that the mouse lineage in question exhibits some deficiency that allows one specific form of cellular dysfunction to accumulate rapidly. (fightaging.org)
  • Here we explore the neuroprotective effect afforded by the absence of sterile alpha and Toll/interleukin-1 receptor motif-containing 1 (SARM1), a prodegenerative NADase, in a rotenoneinduced mouse model of retinal ganglion cell loss and visual dysfunction. (tcd.ie)
  • In stem cells, open chromatin participating in the most significant regression models demonstrated an enrichment for developmental genes and the TAD-forming CTCF binding complex, providing an opportunity for statistical inference of shifting TAD boundaries operating during early development. (elifesciences.org)
  • Though there are many potential confounders to this finding, recent cross-sectional studies in Bolivia have demonstrated significant enrichment for nonagenarians and centenarians at very high altitudes. (fightaging.org)
  • Furthermore, the reseachers performed gene set enrichment analyses (GSEA) comparing mock-treated or infected adult and aged Ifnar1 -/- mice. (news-medical.net)
  • Interestingly, older mice were shown to be more likely to test positive on the "CAM in mice" after surgery and anesthesia than younger mice, recapitulating the finding that elderly patients are at increased risk of developing postoperative delirium. (iars.org)
  • Interestingly, aged wt mice did not show any weight loss compared with adult controls. (news-medical.net)
  • Interestingly, a reduced polyp formation in a gender-specific manner could be observed in both models. (lu.se)
  • Here, we used a gene therapy approach to directly overexpress APPsα in the brain using AAV-mediated gene transfer and explored its potential to rescue structural, electrophysiological and behavioral deficits in APP/PS1∆E9 AD model mice. (springer.com)
  • Neurochemical studies conducted after completing behavioral experiments demonstrated that OBX downregulated the expression levels of cholinergic marker genes encoding choline acetyltransferase and muscarinic M 1 receptor in a manner reversed by ATL and tacrine. (hindawi.com)
  • We found that hearing loss in Df1 /+ mice affected schizophrenia-relevant endophenotypes, including electrophysiological measures of central auditory gain and behavioral measures of auditory sensorimotor gating. (biorxiv.org)
  • So instead of pursuing the futile task of identifying and studying truly delirious animals, Dr. Baxter suggests we refocus our attention on creating behavioral tasks and animal models that are useful to study the disease process itself, a subtle but important distinction. (iars.org)
  • With this in mind, Dr. Baxter recommended using different behavioral models to provide converging lines of evidence, congruent with Dr. Xie's "CAM in mice. (iars.org)
  • The X chromosome-linked mutation resulted in mice ( mdx mice) with high serum levels of muscle enzymes and with histological lesions comparable to those seen in human muscular dystrophy. (hindawi.com)
  • Myostatin, which functions specifically as a negative regulator of skeletal muscle growth, is present at a higher level in serum of cancer cachectic mice than in those of normal healthy mice ( 19 - 21 ). (spandidos-publications.com)
  • Similarly, mice deficient for Msh2 combined with Apc Min demonstrate accelerated rate of tumorigenesis. (wikipedia.org)
  • In ovariectomized mice, uterine regeneration of the DUM transplantation was similarly observed, suggesting that ovarian hormones are not essential for this regeneration process. (jci.org)
  • In this model mutation in the Cdx2 gene in the ApcΔ716 mouse model shifted the formation of the polyps from the intestine to the colon, resembling the human FAP. (wikipedia.org)
  • Mice overexpressing human α-synuclein under the control of the oligodendrocyte specific proteolipid promoter (PLP-α-syn) display aged-dependent progressive neurodegeneration and have been extensively used in the past to assess MSA-related alterations ( 16 - 19 ). (frontiersin.org)
  • This mutation in the murine dystrophin gene caused an absence of dystrophin in skeletal muscle and this key defect validated the mdx mouse as a suitable model of the early onset of DMD human disease [ 5 , 6 ]. (hindawi.com)
  • The overall goal of this centrally categorized mouse method for cecal ligation and puncture, or CLP, is to create a reproducible model of prolonged critical illness with intensive care measures to mimic the human clinical setting. (jove.com)
  • Immunization with human acellular pertussis vaccine, which prevents severe lung infections in the conventional pneumonic infection model, had little effect on nasopharyngeal colonization. (cdc.gov)
  • In pertussis models that emerged from this approach, large numbers of pathogen are introduced deep in the respiratory tract of animals, resembling extreme human infections in their severity and virulence but with more lung involvement than is generally clinically observed. (cdc.gov)
  • To determine the therapeutic efficacy and immunomodulatory effect of an anti-human death receptor 5 (DR5) antibody, TRA-8, in eliminating macrophage subsets in a mouse model of type II collagen-induced arthritis (CIA). (nih.gov)
  • A human/mouse-chimeric DR5-transgenic mouse, under the regulation of a mouse 3-kb promoter and a loxP-flanked STOP cassette, was generated and crossed with an ubiquitous Cre (Ubc.Cre) mouse and a lysozyme M-Cre (LysM.Cre)-transgenic mouse to achieve inducible or macrophage-specific expression. (nih.gov)
  • Chicken type II collagen was used to induce CIA in mice, which were then treated with an anti-human DR5 antibody, TRA-8. (nih.gov)
  • In human/mouse DR5-transgenic Ubc.Cre mice with CIA, transgenic DR5 was most highly expressed on CD11b+ macrophages, with lower expression on CD4+ T cells. (nih.gov)
  • In human/mouse DR5-transgenic LysM.Cre mice, transgenic DR5 was restrictively expressed on macrophages. (nih.gov)
  • In the Df1 /+ mouse model of human 22q11.2 Deletion Syndrome, we find that hearing loss shapes measures that are considered schizophrenia-relevant endophenotypes, such as central auditory gain and auditory sensorimotor gating. (biorxiv.org)
  • Relevant mouse models of human uveal melanoma still remain to be developed. (molvis.org)
  • miR-21 levels were substantially elevated following engraftment and these results were confirmed by quantitative real-time PCR in mouse, pig, and human models of vein graft neointimal formation. (ed.ac.uk)
  • The expression of human CYP2D6 and mouse CYP2D enzymes in humanized and wild-type mice was quantified by immunoblotting and detected at the cellular level by immunocytochemistry. (aspetjournals.org)
  • Mouse CYP2D proteins were detected in brain, allowing, for the first time, a direct comparison of CYP2D expression among mouse, rat, and human brain. (aspetjournals.org)
  • The development of animal models of dengue virus (DENV) infection and disease has been challenging, as epidemic DENV does not naturally infect non-human species. (mdpi.com)
  • Small animal models mimicking human disease could help provide novel insights into the critical pathomechanism that drives the age-dependent SARS-CoV-2 virulence. (news-medical.net)
  • REMFS delayed and reversed cellular senescence in mouse and human cell cultures. (intechopen.com)
  • These applications indicate the feasibility of subsequent practical models for diagnosing and treating human diseases. (intechopen.com)
  • These two pathologies may be present at the same time in human lungs and in mice either instilled with elastolytic enzymes or bleomycin or exposed to cigarette-smoke. (biomedcentral.com)
  • Subsequently, in a proof-of-concept study, mouse models of brain metastasis were induced by intracardial injection of brain-tropic human breast carcinoma, lung adenocarcinoma or melanoma cells to upregulate endothelial ALCAM. (ox.ac.uk)
  • Because PS372424 activates human (but not murine) CXCR3, the O'Boyle/Kirby team had to establish a murine model of human T cell migration. (jax.org)
  • There is no clear human evidence for efficacy of corticosteroids in the treatment of AFM, and there is some evidence in a mouse model with EV-D68 that corticosteroids may be harmful. (cdc.gov)
  • In addition, we measured bead clearance speeds in human ALI, which demonstrated a decrease in bead velocity with aging and a return to baseline after inhibition of PKCe. (cdc.gov)
  • In summary, in human and mouse models, aging is associated with increased oxidant stress, which activates PKCe and slows CBF. (cdc.gov)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • For several of the human tu- and various assays demonstrated an sues. (who.int)
  • Recently, in a transgenic mouse model overexpressing human SHBG, higher TT than wild-type controls but similar FT was demonstrated. (medscape.com)
  • Using a multi-omics approach, we demonstrate that TEs introduce a layer of transcriptome complexity to the human brain. (lu.se)
  • Neuroscience focusing on human development and disease has long been hampered due to ethical rea¬sons, low tissue availability, and low translatability from animal models. (lu.se)
  • Targeting retrotransposons is a new unexplored option for Down syndrome that this work demonstrates is of great therapeutic interest. (scienceblog.com)
  • 2010). Radiobiologists have valued this animal model for sustained grafted tumor growth, which permits fractionation considerations in therapeutic models, even for radiation therapy (Allam et al. (experts.com)
  • extended their groundbreaking discovery demonstrating the protective effect of Txnip on cone photoreceptor survival. (elifesciences.org)
  • On the other hand, adult Ifnar1 -/- Ifngr1 -/- mice lacking functional type I and type II IFN signaling showed severe weight loss and poor survival rates as their aged counterparts. (news-medical.net)
  • Protection of spatial vision is sustained over time in both sexes and is accompanied by increased RGC survival and additionally preservation of axonal density in optic nerves of Sarm1−/− mice insulted with rotenone. (tcd.ie)
  • This particular approach will be demonstrated to characterize a mouse model of vascular cognitive impairment. (bruker.com)
  • Obviously, the abnormal expression of the differentially expressed proteins may directly or indirectly regulate cognitive impairment of the 3xTg-AD mice. (aging-us.com)
  • The first mouse mutant in the Apc gene came from a colony of randomly mutagenized mice. (wikipedia.org)
  • The conditional stabilizing mutation in the beta-catenin gene caused formation of up to 3000 polyps in the small intestine of this mouse model. (wikipedia.org)
  • Aydin D, Weyer SW, Muller UC (2012) Functions of the APP gene family in the nervous system: insights from mouse models. (springer.com)
  • For this work, I have used patient-derived xenograft mouse models and neuroblastoma cells grown as 3D tumor organoids. (lu.se)
  • In the mouse xenograft model of colon cancer, we were able to observe a reduced tumor growth in response to CysLT1R antagonist treatment. (lu.se)
  • We assessed the effects of EQ on peripheral nerve function in the db/db mouse model of type II diabetes. (nature.com)
  • This is the first study describing the production and distribution of ETS-1 and ETS-2 mRNAs and proteins using in situ hybridization and immunohistochemistry in murine ocular tissue sections of normal control eyes and tumoral eyes from mice of the same age. (molvis.org)
  • ETS-1 and ETS-2 mRNA and protein levels were much higher in the ocular tissues of Tyrp-1-TAg mice than in control ocular tissues from wild-type mice. (molvis.org)
  • In the current study, we utilized Stokes and Mueller polarimetry techniques to screen for amyloid-β (Aβ) deposits in formalin-fixed, paraffin-embedded mouse brain tissue at different stages of Alzheimer's disease. (spiedigitallibrary.org)
  • Ethoxyquin (EQ), a quinolone-based antioxidant, has demonstrated neuroprotective properties against several neurotoxic drugs in a phenotypic screening and is shown to protect axons in animal models of chemotherapy-induced peripheral neuropathy. (nature.com)
  • We previously identified ethoxyquin (EQ) as a potent neuroprotective compound through a phenotypic screen against three neurotoxic drugs and demonstrated that it prevents development of peripheral neuropathy in paclitaxel and cisplatin models of chemotherapy-induced peripheral neuropathy (CIPN). (nature.com)
  • The ability of hypoxia to alleviate brain degeneration in such diverse models points either to the pleiotropic effects of oxygen restriction, or alternatively, the existence of a downstream and convergent neuroprotective mechanism. (fightaging.org)
  • The researchers used Ts65Dn mice, the most widely-studied Down syndrome animal model to date. (scienceblog.com)
  • Many researchers worldwide have attempted to elucidate the molecular mechanism underlying muscle wasting using the two models ( 5 - 16 ). (spandidos-publications.com)
  • Researchers here show that a mouse model of accelerated aging lives considerably longer when in a low-oxygen atmosphere for most of its life span. (fightaging.org)
  • Now, researchers at the Broad Institute of MIT and Harvard have found that treatment with continuous hypoxia - low-oxygen conditions comparable to levels at a Mount Everest base camp - restores balance and coordination in a mouse model of Friedreich's ataxia. (broadinstitute.org)
  • To the best of our knowledge, the current study is the first to report that hypoxia extends lifespan in a mouse model of aging. (fightaging.org)
  • We have demonstrated that continuous hypoxia (11% oxygen) - or "oxygen restriction" - significantly extends lifespan of Ercc1 Δ/- mice and delays neurologic morbidity . (fightaging.org)
  • An important future goal is to define the mechanism by which chronic continuous hypoxia is extending lifespan in this model, and the extent to which this mechanism overlaps with that of pathways known to be involved in aging, such as mTOR and insulin signaling . (fightaging.org)
  • We inoculated only the nasopharynx of mice to explore the course of infection in a more natural exposure model. (cdc.gov)
  • Our infection model revealed that B. pertussis can efficiently colonize the mouse nasopharynx, grow and spread within and between respiratory organs, evade robust host immunity, and persist for months. (cdc.gov)
  • Most of our knowledge of B. pertussis has been learned from animal models of pneumonic infection that were developed during an era guided by Koch's postulates ( 8 - 19 ). (cdc.gov)
  • As valuable as conventional high-dose models have been, the bolus introduction of many bacteria deep into the lungs bypasses many key steps in the highly infectious catarrhal stage of pertussis, the prolonged period of early infection involving milder nonspecific upper respiratory tract symptoms. (cdc.gov)
  • Notably, Ifnlr1 -/- mice continued to have high viral titers in their lungs five days post-infection (dpi). (news-medical.net)
  • Conversely, within five dpi, WT mice had cleared the SARS-CoV-2 infection. (news-medical.net)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • Primary fibroblasts extracted from Sarm1−/− mice demonstrate an increased oxygen consumption rate relative to those from wild type mice, with significantly higher basal, maximal and spare respiratory capacity. (tcd.ie)
  • BRS was significantly changed in PLP-α-syn mice and was age-dependent. (frontiersin.org)
  • QT and QTc intervals were not significantly modified in PLP-α-syn mice. (frontiersin.org)
  • Moreover, we found that leaf extract from A. trifoliatus significantly exhibited acute antianxiety effects in animal models by the single oral administration at the concentration of 500 mg/kg body weight [ 6 ]. (hindawi.com)
  • Last month, for instance, Mootha's team showed that a mouse model of an accelerated aging disease lived 50 percent longer and showed slower neurological decline in low-oxygen conditions. (broadinstitute.org)
  • Neurological symptom was observed in G93A mice from 14 weeks by the tail suspension test, and rotarod performances were decreased at 16 weeks and older. (go.jp)
  • This study was carried out in 6 and 12 months old transgenic PLP-α-syn and WT male and female mice. (frontiersin.org)
  • This study investigated long-term outcomes of hyperoxia on retinal morphology and function using a mouse model of oxygen-induced ischemic retinopathy (OIR). (molvis.org)
  • In the present study, we established models of ECC and late cancer cachexia (LCC) and compared different stages of cancer cachexia using two cancer cachectic mouse models induced by colon-26 (C26) adenocarcinoma or Lewis lung carcinoma (LLC). (spandidos-publications.com)
  • In this study, we tested whether T cell exhaustion is recapitulated in the TCL1(tg) mouse model for CLL. (nih.gov)
  • This study investigated effects of the aqueous extract of its leaves (ATL) on cognitive and emotional deficits using an olfactory bulbectomized mouse (OBX) model. (hindawi.com)
  • This phantom mouse design is unique, simple, reproducible, and therefore recommended as a standard approach to dosimetry for radiobiological mouse studies by means of any of the detectors used in this study. (experts.com)
  • To look for other possible regimens, Mootha's team, in their latest study, tested seven different hypoxia treatments in the same mouse model of Friedreich's ataxia and included animals in later stages of the disease. (broadinstitute.org)
  • In this panel, "To Study the Underlying Mechanisms of Postoperative Delirium: Establishment of Clinically Relevant Animal Models" during the IARS 2021 Annual Meeting, experts in the basic science of postoperative delirium weighed in on an important question: can we develop valid animal models to study postoperative delirium, and if so, how? (iars.org)
  • Unfortunately, there is currently no consensus for a valid animal model to study the underlying mechanisms and possible interventions for postoperative delirium. (iars.org)
  • Deborah Culley, MD , Department Chair of Anesthesiology and Critical Care at The University of Pennsylvania, echoed the other panelists' views on the challenges of developing valid animal models to study postoperative delirium. (iars.org)
  • Study 1 investigated the effects of maternal choline supplementation (MCS) on pregnancy outcomes in Dlx3+/- mice. (cornell.edu)
  • This study demonstrated that maternal choline supplementation led to a marked increase in fetal growth by mid-gestation, which resulted in compensatory mechanisms to slow growth by downregulating Igf1. (cornell.edu)
  • Study 2 explored the effects of MCS on aspects of placental function in the Dlx3+/- mouse. (cornell.edu)
  • Study 3 assessed the effects of MCS on vitamin B-12 status in mice with and without placental insufficiency. (cornell.edu)
  • This study was done in two animal models in which emphysema and fibrosis were induced either by bleomycin (BLM) or by chronic exposure to cigarette-smoke. (biomedcentral.com)
  • Humanized NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ ( 005557 ) mice were particularly critical to the success of this study. (jax.org)
  • Histology of the OIR mice exhibited a thin, disorganized structure. (molvis.org)
  • The histology and time course of the disease in mdx mouse model are very different from those in DMD patients: relatively normal life span and overall fitness compared to progressive physical impairment leading to death in DMD patients [ 7 ]. (hindawi.com)
  • In this paper we constructed a simple mathematical model to investigate the role of the immune response in muscle degeneration and subsequent regeneration in the mdx mouse model of Duchenne muscular dystrophy. (hindawi.com)
  • Our model suggests that the immune response contributes substantially to the muscle degeneration and regeneration processes. (hindawi.com)
  • Furthermore, the analysis of the model predicts that the immune system response oscillates throughout the life of the mice, and the damaged fibers are never completely cleared. (hindawi.com)
  • The article [ 13 ] is a comprehensive literature review of the immune-mediated molecular and signaling mechanisms that regulate the time course of the disease and the mdx mouse model. (hindawi.com)
  • Humanized mouse models can sustain DENV replication and show some signs of disease, but further development is needed to validate the immune response. (mdpi.com)
  • Results: MICCop cells were able to inhibit the harmful autoreactive T-cell response and prevented mice from further relapses without affecting general immune responses. (uni-koeln.de)
  • In cell cultures and in "humanized" NOD.Cg- Prkdc scid Il2rg tm1Wjl /SzJ (NSG, 005557 ) mice, they interfered with the immune system's ability to recruit the synovium-attacking T cells. (jax.org)
  • Title : Pulmonary immune responses to Aspergillus fumigatus in an immunocompetent mouse model of repeated exposures Personal Author(s) : Buskirk, Amanda D.;Templeton, Steven P.;Nayak, Ajay P.;Hettick, Justin M.;Law, Brandon F.;Green, Brett J.;Beezhold, Donald H. (cdc.gov)
  • We tested this hypothesis using an established mouse model of diet-induced obesity with pregnancy-associated loss of glucose tolerance and a novel lipid analysis tool, Lipid Traffic Analysis, that uses the temporal distribution of lipids to identify differences in the control of lipid metabolism through a time course. (cam.ac.uk)
  • To do this, they injected sub-lethally irradiated NSG mice with peripheral blood mononuclear cells (PBMCs). (jax.org)
  • Still, radiation control branches and inspectors general have provided approval for dual use with immunodepressed nude mice when infectious disease control measures are properly in place. (experts.com)
  • Informed by studies in infectious disease models, and instructed by a clear role for T RM cells in autoimmunity, we will discuss strategies for therapeutically promoting T RM responses in settings where they don't naturally occur. (frontiersin.org)
  • Studies in infectious disease models have provided a wealth of information regarding memory CD8 T cell generation and localization. (frontiersin.org)
  • This offers significant advantages for studying tumor pathogenesis and molecular mechanisms of oncogenesis caused by a single initiating oncogenic event introduced through the mouse germ line. (molvis.org)
  • We find that elevated basal protein synthesis in Fmr1 KO mice is selectively reduced to wild-type levels by acute inhibition of mGluR5 or ERK1/2, but not by inhibition of mTOR (mammalian target of rapamycin). (jneurosci.org)
  • We also aged wild-type and PKCe KO mice and measured CBF. (cdc.gov)
  • Twenty-two mice were exposed to 77% oxygen to induce OIR, while 23 age-matched control mice were raised in room air (RA). (molvis.org)
  • In 2019, Mootha's team found that continuously exposing young mice with Friedreich's ataxia to 11 percent oxygen, as opposed to 21 percent at sea level, early in the disease prevented ataxia. (broadinstitute.org)
  • Intermittent hypoxia - 16 hours of 11 percent oxygen and 8 hours of 21 percent oxygen - was harmful, and mice developed cardiac stress and died more quickly than those without treatment. (broadinstitute.org)
  • The Min mouse can develop up to 100 polyps in the small intestine in addition to colon tumors. (wikipedia.org)
  • Xenografting is a common technique used by radiobiologists to test the characteristics of disease growth, inhibiting drugs, and tumor responses to drugs and radiation by the injection of tumors and drugs into nude mice. (experts.com)
  • The directed expression of SV40 T antigen has led to the development of several important transgenic models with spontaneous epithelial tumor formation. (molvis.org)
  • Also, a decreased nuclear expression of β-catenin within the epithelial tumor compartment was determined for CysLT1R mutant female mice in both models. (lu.se)
  • Ablation of MLH1 in mice causes development of gastrointestinal tumours in the small intestine - adenomas and invasive carcinomas. (wikipedia.org)
  • DUMs were transplanted into the mouse uteri with artificially induced defects, and all the uterine layers were recovered at the DUM transplantation sites within a month. (jci.org)
  • A truncating mutation at codon 716 (ApcΔ716) results in a mouse that develops more than 300 polyps in the small intestine, while truncation at codon 1638 (Apc1638N) results in the formation of about only 3 polyps in the same region of the gastrointestinal tract. (wikipedia.org)
  • In this case, the model has a mutation that impairs DNA repair . (fightaging.org)
  • An impaired HRV was observed at 12 months of age in PLP-α-syn female but not in male mice, indicative of cardiovascular AF. (frontiersin.org)
  • Male mice received daily oral dose of RP extract or vehicle during the SPS-CF procedure. (lu.se)
  • CysLT1R knockout female mice, but not male mice exhibited a reduced polyp formation in the small intestine and colon, respectively. (lu.se)
  • Each detector system demonstrated results similar to the dose computed by the treatment planning system, although some differences were noted. (experts.com)
  • 3 Because this bone cell culture model demonstrates a capacity to respond to mechanical changes, we hypothesize that this model may also show differences in expression of metalloproteinases in response to cyclic mechanical stretch that can be used to model changes at the bone-implant interface. (allenpress.com)
  • This transgenic mouse model is useful for investigating CYP2D6-mediated metabolism in liver, kidney, and especially the intestine, where expression patterns demonstrated substantial species differences. (aspetjournals.org)
  • To date, for both practical and ethical reasons, studies on muscle wasting have mainly depended on the use of murine models. (spandidos-publications.com)
  • We also demonstrated upregulation of ETS-1 and ETS-2 target expressions in Tyrp-1-TAg mice when comparing with the same target expressions in control mice. (molvis.org)
  • These results demonstrate that lipid metabolism is modulated in healthy pregnancy and the timing of these changes is altered in GDM pregnancies. (cam.ac.uk)
  • Immunohistochemical analysis demonstrated that age-dependent expression of mPGES-1 was found in motor neurons in G93A mice at 11 and 15 weeks. (go.jp)
  • Immunohistochemical analyses confirmed prolonged virus persistence in Ifnar1 -/- Ifnlr1 -/- mice compared with WT mice, with severe damage to lung tissues and bronchial epithelium. (news-medical.net)
  • Dystrophin deficiency does not always produce muscle degeneration at all life stages, in all muscle phenotypes, or in all animal models [ 8 ]. (hindawi.com)
  • 2. Dissect the mechanism of Txnip in RPE vs Cone photoreceptors in retinal degeneration models. (elifesciences.org)
  • In addition, we compared this with the expression of mouse CYP2D enzymes in these organs. (aspetjournals.org)
  • These results suggest that RP exerts some symptomatic relief in a mouse with exaggerated fear response. (lu.se)
  • The DUM transplantation model is a powerful tool for uterine regeneration research. (jci.org)
  • In addition, we found that isolated islets from Chop -/- mice displayed increased expression of UPR and oxidative stress response genes and reduced levels of oxidative damage. (jci.org)