Demonstrate high throughput screening assays. Medical search. Frequent questions

The compact design featuring high power LEDs, large numerical aperture optics, and fast photodiode detection enables all-optical individual well readout of 24-wells simultaneously from multi-well plates while maintaining sufficient temporal resolution to probe millisecond response dynamics. (frontiersin.org)
Hence, our research efforts have focused on developing screening tests capable of multiplexed detection of foodborne bacteria and associated toxins. (usda.gov)
In this study, we describe the combination of a sandwich fluorescence immunoassay with a high-throughput, multiwell plate microarray detection platform. (usda.gov)
The optimized assay had demonstrated limits of detection of ca. 10**5 cells/mL and 100 ng/mL for E. coli O157:H7 and Shiga toxin 1, respectively, in a total assay time of ca. 75 min. (usda.gov)
At the 2007 SBS meeting in Montreal, Dr. Mohammed Kashem from Boehringer Ingelheim Pharmaceuticals, first reported similar findings when he compared Kinase-Glo and a second commercially available ATP-detection luminescent reagent for tolerance to interference by compounds in the BI screening deck. (wtnnews.com)
The reemerging epidemic illustrated the need for epidemiologic investigations of the serologic prevalence and geographic range of hemorrhagic fever viruses, as well as development of novel serologic assays for their detection and surveillance. (cdc.gov)
Serologic assays for detection of antibodies against EBOV glycoprotein (EBOV-GP), matrix protein (VP40), and nucleoprotein (NP) included novel microneutralization and luciferase immunoprecipitation system (LIPS) assays, as well as a commercially available ELISA. (cdc.gov)
Our successful compound screen demonstrates that the split-Rluc assay is an optimal, valid assay for screening, as it readily adapts to a high throughput detection in live cells without artifacts associated with fixation or complications related to image analysis. (biorxiv.org)
Overall, this strategy represents a pipeline for developing efficient and specific assays for virtually any tyrosine kinase that use HTS-compatible lanthanide-based detection. (umn.edu)
A small-molecule library enriched for FDA-approved drugs was screened with a high-throughput, ligation-mediated amplification assay with a fluorescent, bead-based detection. (broadinstitute.org)
While Surface enhanced Raman scattering (SERS) has demonstrated great potential to analyze a variety of bio/chemical molecular interactions within cells in a highly sensitive and selective manner, the main challenge is to ensure high sensitivity and selectivity, while achieving uniform signal enhancement and high reproducibility for the quantitative detection of targeted biomarkers within a complex stem cell microenvironment. (testtechnologypublisher.com)
Using this YaxAB nanopore, we demonstrate label-free, single-molecule detection of interactions between the anticancer Bcl-xL protein and small-molecule drugs as well as the Bak-BH3 peptide. (nature.com)
In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)

The screen shows optimal quality control, maintaining a high sensitivity and specificity withoutcompromising throughput. (whiterose.ac.uk)

One of these compounds (ebselen) also exhibited promising antiviral activity in cell-based assays. (nature.com)
To facilitate the rapid discovery of antiviral compounds with clinical potential, we developed a strategy that combines structure-assisted drug design, virtual drug screening and high-throughput screening to repurpose existing drugs to target SARS-CoV-2 M pro . (nature.com)
Pharmacologic activation of STING has been demonstrated to induce an antiviral state and boost antitumor immunity. (elsevierpure.com)
To aid the identification of effective therapeutics for LUJV, we developed a recombinant reporter virus system, confirming reporter LUJV comparability with wild-type virus and its utility in high-throughput antiviral screening assays. (cdc.gov)

Using a fluorescence based readout of neuronal stress, we developed a high throughput (HT) screen to identify neuroprotective compounds. (whiterose.ac.uk)
To characterize the enzymatic activity of SARS-CoV-2 M pro and to carry out high-throughput screening of inhibitors, we developed a fluorescence resonance energy transfer assay. (nature.com)
In this reporter system when sE activity is high, RybB will be produced, and there will be low fluorescence. (psu.edu)
if sE activity is low, ompC-yfp transcription will be undisturbed and high fluorescence will be observed. (psu.edu)

This homogeneous, bioluminescent assay is easily scalable to 1,536-well plate formats. (genengnews.com)
Ultra-Glo was derived by directed evolution and is the key component in Kinase-Glo Assay and a suite of other bioluminescent assays for high throughput screening and profiling of small molecule compound libraries. (wtnnews.com)
However, with existing bioluminescent reporter technology, this is simply not possible because the current market of bioluminescent reporter cells being applied toward toxicology screening relies upon a firefly luciferase gene construct that must be provided with a chemical substrate to activate its light emission response, resulting in only marginally informative single time point snapshots of potential toxicological interactions. (inknowvation.com)
To overcome these detractions and develop an improved tier 1 screening system that reduces the cost and time required for new compound evaluation, 490 BioTech proposes to develop a panel of multiple continuously bioluminescent human cell lines that will permit the simultaneous monitoring of each line to ascertain both the individual effects of compound treatment as well as the downstream effects of a compound's biotransformed metabolic breakdown products in real-time. (inknowvation.com)

Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
Clear broth extract was incubated with in vitro cultures of Plasmodium falciparum asexual or mature sexual stage cultures and assayed for parasite viability after 72 hours. (bmj.com)
Several compounds tested in secondary cell viability assays also demonstrate cytoprotective effects. (nyu.edu)

Sound energy enables the dispensing of droplets of test compounds in 2.5-nL increments directly from source plates to the assay plates. (genengnews.com)
After the compounds are dispensed into the assay plates, cells of interest are added. (genengnews.com)
Most importantly, we demonstrate that many compounds previously failed in human clinical trials, showed no stress reducing activity in the zebrafish assay. (whiterose.ac.uk)
Here we describe the results of a programme that aimed to rapidly discover lead compounds for clinical use, by combining structure-assisted drug design, virtual drug screening and high-throughput screening. (nature.com)
Through a combination of structure-based virtual and high-throughput screening, we assayed more than 10,000 compounds-including approved drugs, drug candidates in clinical trials and other pharmacologically active compounds-as inhibitors of M pro . (nature.com)
We demonstrated that this cell line responds to compounds typically used in the field of sGC research and that it represents an innovative and robust assay to screen for sGC modulators with high efficiency and high sensitivity by means of standard luminescence readers. (nih.gov)
Routine laboratories will benefit from Thermo Scientific TraceFinder 5.1 software, which simplifies and optimizes the high-throughput screening and quantitation of compounds with improved integration of difficult peaks and streamlined reporting. (technologynetworks.com)
We identified six compounds demonstrating robust anti-LUJV activity, including several compounds with previously reported activity against other arenaviruses. (cdc.gov)

Future studies will begin linking the transwell assay to higher content chemical screening capabilities to increase throughput. (cdc.gov)

Primary hepatocytes, the most common in vitro model for drug-induced liver toxicity testing, are not ideal for high throughput screening due to their limited availability, difficulty to culture, and functional instability. (rndsystems.com)
In this study, we demonstrate the reliability and efficiency our StemXVivo ® Hepatocyte Differentiation Kit , which uses a standardized protocol with high quality reagents to generate functional hepatocyte-like cells from human pluripotent stem cells. (rndsystems.com)
therefore, cellular and molecular analyses of RTX and CA125 interaction were peformed, and a library of RTX variants was generated using a proprietary technology called Block‑Removed Immunoglobulin Technology that combines randomized amino acid substitutions and high‑throughput functional screenings to identify CA125‑refractory RTX variants. (spandidos-publications.com)
Analyses in multiple orthogonal assays validated that these agonists promote physical and functional interactions between the two organelles. (biorxiv.org)
We addressed this issue by experimentally demonstrating the existence of functional disrupted genes in archaeal genomes. (nih.gov)

To demonstrate suitability of the panels for high throughput screening, the EGFR panel was selected for evaluation using Corning Epic Technology, a label-free platform that uses optical biosensors for high sensitivity biochemical and cell-based assays. (atcc.org)

These results demonstrate that the StemXVivo ® Hepatocyte Differentiation Kit provides a reliable and renewable source of hepatocyte-like cells that can be utilized for high throughput toxicology and drug discovery. (rndsystems.com)

This article highlights a system that combines acoustic dispensing, small-volume pipetting, and robust assay chemistries to identify GPCR modulators. (genengnews.com)
Implementing this miniaturized, high-throughput GPCR assay allows cost-effective screening to identify lead modulators of GPCR signaling. (genengnews.com)
In order to find new modulators for this important enzyme, an innovative cell-based assay has been developed and optimized for the use in high-throughput screening. (nih.gov)
Here we screened a compound library to identify chemical modulators for ER-Mito contacts. (biorxiv.org)

There are now a number of publications demonstrating successful application of Epic for G proteincoupled receptors (GPCRs), where DMR profiles across many different cell backgrounds are highly characteristic of the downstream signaling pathway. (atcc.org)
We provide experimental evidence demonstrating that ebastine has antitumor activity in osteosarcoma and promotes autophagy by activating the AMPK/ULK1 signaling pathway, which is IPMK dependent. (ijbs.com)

Non-invasive saliva human biomonitoring: development of a transwell assay to support exposure science assessment and health impacts. (cdc.gov)
Serous-acinar cells express the tight junction marker zona occludens-1 and measurements of transepithelial electrical resistance (TEER) demonstrate exceptional tight junction formation [routinely >2000 omega(ohms)/cm2] in a relatively short period of time (6-8 days) in Transwell inserts. (cdc.gov)
Results from the Transwell assay indicate that chlorpyrifos transports by diffusion with transport rates that are linear among doses tested. (cdc.gov)

An assay to identify inhibitors of a conserved bacterial stress response pathway, sigma factor sE, has been developed. (psu.edu)
No inhibitors are currently avaibale for this sE pathway and as assays can currently identify such inhibitors. (psu.edu)
The innovative primary screen and secondary assays can be readily adapted to identify inhibitors of other sRNA regulators and sigma factors or other transcriptional regulators. (psu.edu)
Both diagnostic assays to test for defects in this DNA repair pathway and HER2 inhibitors are FDA-approved in cancer patients and can be easily used in the breast cancer context. (sbpdiscovery.org)

The Swarm can screen ~10,000 wells/day in 384-well format, probing complex pharmacological interactions via a wide array of stimulation protocols. (frontiersin.org)
The drug discovery process requires that upwards of 10,000 molecules be screened for each new lead compound developed and relies upon an expensive and time consuming combination of in vitro cell culture-based and in vivo whole animal-based models to identify, validate, and ensure the safety of any resulting potential therapeutic agents. (inknowvation.com)

Our protocol demonstrates how to produce honey bee virus particles en masse for their use in controlled, high-throughput bioassays for rapid screening of viral treatment effectiveness. (jove.com)

The high-throughput character and feasibility in SELDI-TOF-MS experimental methods produced this technology helpful for biomarker finding [23 24 for the characterization of different cell populations (T-cell and fibroblast) [25 26 and with an excellent potential for medical perspectives. (sciencepop.org)
Experimental results from a leading pharmaceutical company have demonstrated the feasibility of this assay. (psu.edu)
We probed putative mechanisms and demonstrated clinical feasibility and activity of a putative combination by treating two patients with active, r/r-T-PLL. (haematologica.org)

Actually the hierarchical clustering analyses of proteins profile demonstrated substantial inter-individual differences which were consistent with earlier data obtained through the use of additional approaches as microarray assays [28]. (sciencepop.org)

Finally, longer term assay formats such as cytotoxicity, viral infection and cellular differentiation assays are all feasible using Epic. (atcc.org)
Cellular studies of NTD and CTD oligomerization interface mutants demonstrate the importance of each interface in matrix assembly. (uci.edu)
However, most existing technologies for assay of cellular lipids possess poor sensitivity and require large sample volumes making them unsuitable for the assay of mammalian cells which are 1 pL in volume and may possess as little as 10-20 moles of key regulatory lipids. (selectbiosciences.com)
This technology can be used for high-quality and high throughput bio/chemical molecule screening assays as well as to understand cellular phenomena such as disease progression and stem cell differentiation. (testtechnologypublisher.com)

Taken together, these findings warranted further investigation on NAV‑006 as a next generation anti‑CD20 antibody that could improve the efficacy of parent RTX in NHL patients with high levels of CA125. (spandidos-publications.com)
We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs. (bvsalud.org)
This report describes generation of a recombinant Sudan virus and demonstrates its utility by quantifying antibody cross-reactivity between Ebola and Sudan virus glycoproteins after human infection or vaccination with a licensed Ebola virus vaccine. (cdc.gov)

This initiative is one of the integrated components of the NIH Molecular Libraries Roadmap initiative that offers biomedical researchers access to large-scale automated high throughput screening (HTS) centers in the MLPCN, diverse compound libraries in the Small Molecule Repository (MLSMR) and information on biological activities of small molecules in the PubChem BioAssay public database. (nih.gov)
The NCGC is leading the US government-sponsored efforts aimed at developing biological probes and expanding the use of HTS assays in academic settings. (wtnnews.com)
The last two decades have witnessed major breakthroughs in the identification of genes, gene products, metabolic pathways, and signaling pathways, as well as progress in miniaturization and robotics, enabling the development of high-throughput mechanism-based biological assays. (mdpi.com)

Concurrently, we analyzed patient serum samples before and 1 week after 3 weekly injections of CLBS20, using comprehensive high-throughput screening for proteins responsible for immune response, inflammation and angiogenesis. (bmj.com)
Using a newly developed enzyme-coupled continuous activity assay, we compare differing NAS proteins identified through multiple sequence alignments and phylogenetic analyses. (bvsalud.org)
The long funnel-shaped structure and nanofluidic characteristics of the YaxAB nanopore enable the electro-osmotic trapping of diverse folded proteins and high-resolution monitoring of PDIs. (nature.com)
http://toxsci.oxfordjournals.org/ node assay (LLNA) is viewed as the most appropriate skin react with proteins for the effective induction of skin sensitization. (cdc.gov)

In order to sift through today's complex compound libraries, drug developers need high-throughput assays that are easy to use, sensitive, precise, cost-effective, and able to accurately identify promising drug candidates quickly. (genengnews.com)
Use this information to develop high-throughput assays to screen for drug candidates. (transcellonco.science)
High-throughput screening of drug candidates for channelopathies can greatly benefit from an automated patch-clamping assay. (iitj.ac.in)

Optogenetic assays provide a flexible, scalable, and information rich approach to probe compound effects for ion channel drug targets in both heterologous expression systems and associated disease relevant cell types. (frontiersin.org)
Despite the potential utility and growing adoption of optogenetics, there remains a critical need for compatible platform technologies with the speed, sensitivity, and throughput to enable their application to broader drug screening applications. (frontiersin.org)
Zebrafish is an emerging model system that is well suited for the study of diseasepathophysiology and also for high throughput (HT) drug screening. (whiterose.ac.uk)
Conclusion: We conclude that HT drug screening using a mutant sod1 zebrafish is a reliable model system which supplemented with secondary assays would be useful in identifying drugs with potential for neuroprotective efficacy in ALS. (whiterose.ac.uk)
As the technology has matured, intestinal organoids have found application in a wide variety of research fields, including upstream drug discovery and patient-specific drug screening, cancer and immunology studies, and the pathogenesis of infectious agents. (stemcell.com)
Our results demonstrate the efficacy of our screening strategy, which can lead to the rapid discovery of drug leads with clinical potential in response to new infectious diseases for which no specific drugs or vaccines are available. (nature.com)
Zebrafish are used extensively in disease modeling and drug development due to their high level of genetic conservation, associated disease phenotypes, and ability to rapidly reproduce. (axionbiosystems.com)
This enables us to provide high-quality data for veterinary drug residue testing to our clients. (technologynetworks.com)
The program focuses on the early stages of the drug discovery process, from target validation through "proof-of concept" studies, which aims to demonstrate that a newly discovered drug candidate can successfully alter the course of cancer disease progression. (sbpdiscovery.org)
With current in vitro screening assays now representing a $1.4B market with a predicted 12% annual growth rate, we believe we possess a product capable of significantly impacting the chemical/drug screening market and, here in particular, advancing our understanding of cytotoxic chemical biotransformations as they pertain to public health and consumer safety. (inknowvation.com)
In drug discovery, efficient screening of protein-drug interactions (PDIs) is hampered by the limitations of current biophysical approaches. (nature.com)
Current drug development suffers from high cost and low efficiency of the drug discovery process. (nature.com)
Thus, there is a high demand for low-cost, efficient drug screening technologies against them. (nature.com)
We employed combinatorial drug screening to identify synergistic combination partners to enhance the efficacy of venetoclax in T-PLL patients. (haematologica.org)
Drug screening was performed as previously described. (haematologica.org)

Demonstrate safety and efficacy in clinical trials in order to satisfy the approval requirements of regulators, and deploy in the marketplace to benefit patients. (transcellonco.science)
r\nDiscussion: The results of this review will provide clinicians with an unbiased, high level of evidence of the\r\ncomparative efficacy of individual preparations of botulinum toxin A. (inventi.in)
Recently, we and others have demonstrated in vitro activity and clinical efficacy of the Bcl-2 inhibitor venetoclax as a single agent in relapsed/refractory T-PLL (r/r-TPLL). (haematologica.org)

The NIH Molecular Libraries Roadmap Initiative wishes to solicit HTS assay applications from the scientific community from investigators who have the interest and capability to work with the Molecular Libraries Probe Production Centers Network (MLPCN) in support of chemical probe development. (nih.gov)
We hypothesize that screening small-molecule libraries highly enriched for FDA-approved drugs will provide a more rapid path to clinical application. (broadinstitute.org)

MADISON Research results just published in the Journal of Medicinal Chemistry demonstrate the superior performance of Promega Ultra-Glo™ Luciferase. (wtnnews.com)
This program brings together the complementary strengths and resources of its members and provides expertise in assay development, high throughput screening, downstream medicinal chemistry and pharmacology through its partnership with the Prebys Center. (sbpdiscovery.org)

Results: Here we show that the zebrafish screen is a robust system that can be used to rapidly screen thousands ofcompounds and also demonstrate that riluzole is capable of reducing neuronal stress in this model system. (whiterose.ac.uk)

The studies reveal why most screeners prefer Ultra-Glo as the luciferase enzyme formulation in their screening and profiling regimens. (wtnnews.com)
The tools provided in the pipeline also have the potential to be adapted to identify peptides for other purposes, including other enzyme assays or protein-binding ligands. (umn.edu)

Here we describe two protocols: first to propagate, extract, purify, and quantify large quantities of honey bee non-enveloped virus particles, including a method for removing honey bee pupae and second to test the effects of viral infection using a highly repeatable, high-throughput cage bioassay. (jove.com)

The combination of a RT-PCR strategy with shotgun proteomics demonstrates that interrupted genes in the archaeon Sulfolobus solfataricus are expressed in vivo. (nih.gov)
1 T-PLL cells commonly demonstrate rearrangements involving T-cell leukemia/lymphoma 1 (TCL1) family genes TCL1A, MTCP1 (mature T-cell proliferation), or TCL1B as molecular hallmarks. (haematologica.org)

The cAMP-Glo assay can be performed in standard multiwell formats, including 96-, 384- or 1,536-well plates. (genengnews.com)

PBMC fraction also contains progenitor populations, as demonstrated by methylcellulose based colony forming assays. (wikipedia.org)

The OrganoPlate ® is a high-throughput platform that combines the most recent advances in 3D cell culture, Phaseguides™ and microfluidics 4,5,6 . (moleculardevices.com)
Cell culture, compound treatment, and cell staining steps were performed using the microfluidics assay format. (moleculardevices.com)

To address this challenge, we developed the Swarm TM , a custom designed optical instrument for highly parallelized, multicolor measurements in excitable cells, simultaneously recording changes in voltage and calcium activities at high temporal resolution under optical stimulation. (frontiersin.org)
As a demonstration of the versatility of the Swarm, we also developed an assay measuring cardiac action potential and calcium waveform properties simultaneously under paced conditions using human induced pluripotent stem (iPS) cell-derived cardiomyocytes as an additional counter screen for cardiac toxicity. (frontiersin.org)
The Orbitrap Exploris 120 mass spectrometer was designed specifically for laboratories to safeguard their current assays and enhance their provision of high-throughput and differentiated services, while simultaneously minimizing required training and method development times. (technologynetworks.com)
This problem could be significantly mitigated by coordinately screening multiple tissue types simultaneously in a high-throughput fashion during upstream tier 1 screening. (inknowvation.com)

Peptide substrates that can enhance lanthanide ion luminescence upon tyrosine phosphorylation enable rapid, sensitive screening of kinase activity, however design of suitable substrates that can distinguish between tyrosine kinase families is a huge challenge. (umn.edu)

In this webinar, we will discuss how the combination of Epic Technology and the EGFR Genetic Alteration Panel offers convenient tools to screen for ligands or biologics that directly target or affect EGFR receptor biology. (atcc.org)
In this webinar, Dr. Nadja Zeltner and Dr. Vicente Kenyi Saito-Diaz (University of Georgia) discuss how their lab developed a protocol to derive enriched populations of peripheral neuronal subtypes which they characterized using the Maestro multielectrode array assay. (axionbiosystems.com)
In this webinar, Dr. Danielle Tomasello (Whitehead Institute for Biomedical Research) , describes an easy-to-use method to measure neural activity in living zebrafish larvae using microelectrode arrays, or MEAs, and demonstrates it by measuring responses to valproic acid in the brain and spinal cord. (axionbiosystems.com)
In this webinar, Dr. Patapia Zafeiriou (University Medical Center Goettingen) discusses how her lab used a Maestro multielectrode array assay to record the complex neural network activity from Bioengineered Neural Organoids (BENOs). (axionbiosystems.com)
On the phone dialing Star 1 will put you in the queue for questions and you may submit questions through the Webinar system at any time during the presentation by selecting the Q&A tab at the top of the webinar screen and typing in your question. (cdc.gov)

The OrganoPlate contains 96 tissue chips suitable for longterm culture of live cells, is amenable for screening purposes, and is compatible with standard laboratory equipment or automated systems, like the ImageXpress ® Micro Confocal High-Content Imaging System. (moleculardevices.com)
We demonstrated the approach by developing several substrates that are selective within kinase families and amenable to high-throughput screening (HTS) applications. (umn.edu)
Unfortunately, many classes of oncoproteins (e.g., transcription factors) are not amenable to conventional small-molecule screening. (broadinstitute.org)

Cells and subsequent assay reagents can be dispensed into assay plates using a non-contact, liquid dispenser (Equator™ HTS, Deerac Fluidics ) for accurate pipetting. (genengnews.com)

This luminescent assay, which is suitable for both 96- and 384-well plate formats, has been achieved by stably expressing the alpha and beta subunits of a mutated form of sGC in Chinese hamster ovary cells. (nih.gov)
Moreover, we demonstrate that the Biolog metabolic assay is suitable for high-throughput antimicrobial susceptibility screening of H. pylori. (montana.edu)

Many scientists conducting research in the fields of immunology (including auto-immune disorders), infectious disease, hematological malignancies, vaccine development, transplant immunology, and high-throughput screening are frequent users of PBMCs. (wikipedia.org)
Though it is a useful model for neural development and neurological disease, achieving high quality electrophysiological readings from live zebrafish can still be a challenge. (axionbiosystems.com)
While iPSC-derived neurons show great promise for compound screening and disease modeling, use of three-dimensional (3D) cultures is emerging as a valid approach for neuronal cell based assay development. (moleculardevices.com)
Furthermore, the development of lanthanide-based kinase assays is hampered by incomplete understanding of how to integrate sequence selectivity with metal ion binding, necessitating laborious iterative substrate optimization. (umn.edu)
Assay development experience. (astrixinc.com)
The results of these investigations reveal that measurement of peptide reactivity has considerable potential the development of alternative methods for skin sensitization utility as a screening approach for skin sensitization testing, and testing. (cdc.gov)
Allergic contact dermatitis (ACD) resulting from skin have undertaken recently the development of chemical re- sensitization is a critical toxicological endpoint evaluated for activity screening methods for aiding in the assessment of all new chemicals developed for consumer and/or occupational a chemical's skin sensitization potential (Aptula et al. (cdc.gov)

The assay exhibits high reproducibility and precision and works well in 384-well plates and in 5 μl to 20 μl samples. (illinois.edu)

Recent clinical evidence suggests that high serum levels of the tumor‑produced mucin 16 (MUC16) and cancer antigen 125 (CA125) have a negative impact on the effectiveness of RTX clinical activity in up to 40% of patients with follicular lymphoma. (spandidos-publications.com)
Previous clinical evidence suggested that high levels of serum CA125 have a negative impact on the effectiveness of the experimental therapeutic antibodies farletuzumab and amatuximab ( 13 - 15 ). (spandidos-publications.com)
Key evidence showing that ADCC plays an important role in the clinical effect of RTX was provided by the analysis of Fc gamma RIIIa (CD16a) polymorphisms, where patients carrying CD16a 158V/V genotype (associated with high affinity Fc gamma receptor) showed significantly improved objective response rates compared with FL patients carrying at least one 158F allele (associated with low affinity Fc gamma receptor) ( 20 ). (spandidos-publications.com)
Then, I will discuss several applications of our modular systems and the assays for selection of rare liquid biopsy targets from clinical samples and molecular analysis of their contents to help guide disease management. (selectbiosciences.com)
We demonstrate that a gene expression-based approach to small-molecule library screening can identify, for rapid clinical testing, candidate drugs that modulate previously intractable targets. (broadinstitute.org)
While numerous clinical trials demonstrated success in long-term survival using immunotherapy agents in various types of malignancies, very few have demonstrated the underlying mechanism of action of these therapies. (bmj.com)
This process is frustratingly exacerbated by an unfortunate dichotomy whereby the inexpensive in vitro cell culture systems used for tier 1 screening contribute to failures due to their inabiliy to model the complexity and parallel systems interaction inherent in whole animal models (which are responsible for up to 92% of new compound failures at the clinical level) and because they are not capable of demonstrating species-specific effects. (inknowvation.com)
No tumor markers with high sensitivity and high specificity for endometrial cancer are known at present, although CA-125 is often used in clinical practice. (medscape.com)
Conse- demonstrated that this protein signature provided an added value quently, using minute amounts of nonfractionated serum (14) and compared with conventional clinical parameters. (lu.se)

We screened serum samples for neutralizing antibodies by using a pseudotype microneutralization assay and a newly developed luciferase immunoprecipitation system assay. (cdc.gov)
High serum level of CA125 is used as an indicator of tumor abdominal involvement, advanced staging, relapse rates and survival in NHL patients ( 8 - 12 ). (spandidos-publications.com)
The analysis of cytokines demonstrated a decrease of serum tumor markers and the reduction of Treg response in the treatment arm, compared to the control arm, and the results suggest a combination of humoral and cell immune responses. (bmj.com)
The results from this prevali- the field of cancer biomarkers (11-13), and that approach was dation study showed that patients could be classified into high- adopted here to define predictive serum biomarkers associated versus low-risk groups for developing metastatic breast cancer with tumor relapse in breast cancer patients. (lu.se)

The assay is based on the principle that cAMP stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction (Figure 1). (genengnews.com)
The present study demonstrated that CA125 could bind to RTX and reduce its tumor cell killing activity. (spandidos-publications.com)
We found that PF-6870961 had no off-target interactions and demonstrated both binding affinity and inverse agonist activity at GHSR1a. (uri.edu)
Intraperitoneal injection of PF-6870961 suppressed food intake under conditions of both food restriction and with ad libitum access to food in male and female rats, demonstrating in vivo activity. (uri.edu)
Our findings indicate that the newly discovered major hydroxy metabolite of PF-5190457 may contribute to the overall activity of PF-5190457 by demonstrating inhibitory activity at GHSR1a. (uri.edu)
Our data demonstrate biased inverse agonism of PF-6870961 at GHSR1a and provide new structure-activity relationship insight into GHSR1a inverse agonism. (uri.edu)
Antimicrobial activity was higher for extracts than powders and appeared to be independent of the anthocyanin concentration. (montana.edu)
Conclusions This screening approach succeeded in finding broths with activity against malaria parasite in vitro growth, arising from complex vegetable and/or meat-based broths. (bmj.com)
6 In addition, independent annexin V/Hoechst assays of primary T-PLL cells on NK-Tert stromal support demonstrated modest single-agent activity of ibrutinib ( Figure 1D , Online Supplementary Figure S2A ). (haematologica.org)

The new Thermo Scientific Orbitrap Exploris 120 mass spectrometer delivers demonstrated qualitative and quantitative capabilities synonymous with Orbitrap high-resolution accurate-mass spectrometry (HRAM), that supports consistent data quality and decision making. (technologynetworks.com)

Through its funding of the MLPCN, NIH will support the costs of assay automation, screening and optimization chemistry. (nih.gov)

Kit-derived hepa tocyte-like cells were evaluated for use as a high throughput in vitro model for liver toxicity by assessing their response to known hepatotoxic molecules using a high-content imaging assay. (rndsystems.com)

Adherent, suspension, or frozen cell cultures can be used with the cAMP-Glo Assay. (genengnews.com)
What other types of targets has Corning Epic Technology been used for in cell-based assays? (atcc.org)
Epic has been used successfully for a wide range of targets in cell-based assays. (atcc.org)
The assay uses easy-to-culture bee pupae rather than cell culture, reducing the labor of obtaining experimental subjects. (jove.com)
To evaluate the Swarm screening system, we optimized a series of heterologous optogenetic spiking HEK293 cell assays for several voltage-gated sodium channel subtypes including Nav1.2, Nav1.5, and Nav1.7. (frontiersin.org)
Beyond this protective role, Mrr can inflict chromosomal DNA damage that elicits the SOS response in the host cell upon heterologous expression of specific methyltransferases such as M.HhaII, or after exposure to high pressure (HP). (uci.edu)
To demonstrate the power of pTLC, leukemic cells were loaded with fluorescent PC, PE or sphingosine and a single cell printed at the inlet of a pTLC channel. (selectbiosciences.com)
The ideal candidate will have post-doctoral and industry experience in single cell genomics and have been involved in high throughput screening and lead candidate selection. (juvenatherapeutics.com)
Juvena's approach integrates proteomics, transcriptomics and imaging with phenotypic human in vitro disease model screening and extensive preclinical validation to mine stem cell secretomes as a rich source of new medicines for chronic and rare diseases. (juvenatherapeutics.com)
Immune cell infiltration analysis suggested that patients with idiopathic pulmonary fibrosis had a higher level of B cells memory, Plasma cells, T cells CD8, T cells follicular helper, T cells regulatory (Tregs), Macrophages M0, and Mast cells resting compared with the control group. (bvsalud.org)
We've screened more than 1,275 cancer cell lines as part of the Cancer Dependency Map (DepMap). (broadinstitute.org)
Identification of these mutated antigens allows us to further study specific T cell activation using mixed lymphocyte reaction assays. (bmj.com)
We previously reported a cell-based high-throughput-screening assay that allowed for identification of small-molecule cGAS-STING-pathway agonists. (elsevierpure.com)

We analyzed suitability of the microplate-based FA assay for high-throughput screening for small-molecule regulators of RNA-protein interactions. (illinois.edu)

Data are limited in that previous serologic surveys for filoviruses date back several decades ( 7 - 9 ) or have focused on specific populations and locations with high exposure to potential risk factors ( 6 , 10 ). (cdc.gov)

Thermo Fisher Scientific's newest Orbitrap high-resolution mass spectrometer is designed to deliver proven measurement capabilities in a system developed for increased productivity. (technologynetworks.com)

The focus of the present study was to develop a high-throughput 3D neurite outgrowth assay using iPSCderived neurons developed in the microfluidic OrganoPlate ® platform, with the goal of establishing 3D models for neurodegenerative diseases and neurotoxicology screens 3 . (moleculardevices.com)

Interchangeable deck components, including small- or large-volume capacity reservoirs, stirring devices, and multiple wash stations, or other customizable configurations, make this a flexible platform for high-throughput screening (HTS). (genengnews.com)
Signature-based small molecule screening identifies cytosine arabinoside as an EWS/FLI modulator in Ewing sarcoma. (broadinstitute.org)
Previously, this target has been intractable to modulation with traditional small-molecule library screening approaches. (broadinstitute.org)
Our data here suggest that a screening protocol based on lipid-catalyzed assembly will find mechanistically informative small molecule hits in this subclass of amyloid diseases. (nyu.edu)
Furthermore, by leveraging human cells as the sensing platform, our assay provides more accurate and realistic information in regards to bioavailability and a chemical's true effect on individual human health than does the employment of small animal models. (inknowvation.com)

Thishighlights the need for compound screening in disease models to identify new neuroprotective therapies for this disease. (whiterose.ac.uk)
to identify individuals who are at high focus on identification of uncommon enough risk that they are likely to benefit or rare genetic variants that may have With the start of the new IARC Medium- from potential screening strategies. (who.int)

The assay measures cAMP levels in cells by monitoring cAMP production in response to the effects of a test compound on GPCRs. (genengnews.com)
Correlation analysis demonstrated that FHL2 was significantly associated with the infiltrating immune cells. (bvsalud.org)
In a proof-of-concept study, it was demonstrated that the progression of differentiation of stem cells into neurons can be tracked using specific mRNAs markers. (testtechnologypublisher.com)

Anatomy7

Organisms9

Diseases1

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment99

Phenomena and Processes57

Disciplines and Occupations19

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture13

Information Science29

Health Care11

Detection13

Sensitivity and specifi1

Antiviral4

Fluorescence4

Bioluminescent4

Viability3

Compounds8

Increase throughput1

Functional5

Biochemical1

Toxicology1

Modulators4

Signaling pathway2

Transwell3

Inhibitors4

10,0002

Bioassays1

Feasibility3

Microarray1

Cellular4

Antibody3

Biological3

Proteins4

Candidates3

Drug15

Efficacy3

Libraries2

Medicinal chemistry2

Robust1

Enzyme2

Viral infection1

Genes2

Multiwell1

Colony1

Microfluidics2

Simultaneously4

Luminescence1

Webinar5

Amenable3

Reagents1

Suitable2

Development7

Reproducibility1

Clinical9

Serum4

Activity9

Quantitative1

Automation1

Molecules1

Cell13

Suitability1

Populations1

Capabilities1

Develop1

Small5

Identify2

Cells3