• We used whole-genome sequencing of multiple isogenic chicken DT40 cell clones to precisely determine the consequences of BRCA1/2 loss on all types of genomic mutagenesis. (crick.ac.uk)
  • Our results resolve the outstanding question of how hybrids in native genomic contexts interact with replication to cause mutagenesis and shape genome organization. (biorxiv.org)
  • Two main strategies have been employed to identify essential genes on a genome-wide basis: directed deletion of genes and random mutagenesis using transposons. (wikipedia.org)
  • In transposon-mediated mutagenesis, transposons are randomly inserted in as many positions in a genome as possible, aiming to disrupt the function of the targeted genes (see figure below). (wikipedia.org)
  • Another mutant strain was also constructed through partial deletion of the 5' NTR region in the EV-A71 genome. (sunway.edu.my)
  • New Breeding Techniques (NBTs) include several new technologies for introduction of new variation into crop plants for plant breeding, in particular the methods that aim to make targeted mutagenesis at specific sites in the plant genome (NBT mutagenesis). (frontiersin.org)
  • instead, a convergence has taken place between conventional mutagenesis and NBTs, in particular due to the possibilities of TILLING methods that allow the fast detection of mutations in any gene of a genome. (frontiersin.org)
  • Here, we investigate the contributions of RNases HII (RnhB) and HIII (RnhC) to hybrid removal, DNA replication, and mutagenesis genome-wide. (biorxiv.org)
  • In Schizosaccharomyces pombe (fission yeast) 4,836 heterozygous deletions covering 98.4% of the 4,914 protein coding open reading frames have been constructed. (wikipedia.org)
  • We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. (elifesciences.org)
  • Site-directed mutagenesis facilitates life science research by creating specific DNA mutations (including point mutations, deletion mutations, and insertion mutations) to enable studies of gene regulatory elements, DNA-protein interactions, protein structure/function, enzyme active sites, and novel proteins. (genscript.com)
  • Our technology is optimized to introduce point mutations, insertion mutations, and deletion mutations on constructs as large as 12 kb (including target gene and its vector). (genscript.com)
  • We reasoned that it would be worthwhile to establish a generally applicable reverse genetic technique based on an unbiased and practicable random mutagenesis and an efficient mutation-detection performed on standard laboratory equipment. (biomedcentral.com)
  • Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL region of the biotype I octopine strain Ach5 and 'small TA' strains with a similar T region organization as the 'large TA' strains but with a large internal TA deletion. (nih.gov)
  • Therefore, the perceived virulence of WNV deletions existed in the 3 noncoding region of 2 lineage 2 in recent epidemics probably refl ects high medical alert- strains previously shown to be either less or not neuroinva- ness, active surveillance programs, and the emergence and sive relative to the highly neuroinvasive strains sequenced reemergence of existing strains of WNV in locations with in this study. (cdc.gov)
  • A difference in the short deletion phenotypes of BRCA1 and BRCA2 suggested distinct roles for the two proteins in the processing of DNA lesions, as BRCA2 mutants contained more short deletions, with a wider size distribution, which frequently showed microhomology near the breakpoints resembling repair by non-homologous end joining. (crick.ac.uk)
  • It was observed that mutants 475 and the partial deletant (deletion from nt. (sunway.edu.my)
  • Methods: Clones indicate single gene deletions, population indicates whole population mutagenesis, e.g. using transposons. (wikipedia.org)
  • Gene Deletion" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • This graph shows the total number of publications written about "Gene Deletion" by people in this website by year, and whether "Gene Deletion" was a major or minor topic of these publications. (umassmed.edu)
  • Below are the most recent publications written about "Gene Deletion" by people in Profiles. (umassmed.edu)
  • Pioneering work in the late 1990s through early 2010s resulted in vectors for gene deletion and expression in Clavibacter , but there is still reliance on these tools despite known limitations. (biorxiv.org)
  • Using our sophisticated gene synthesis and mutagenesis techniques, GenScript can introduce mutations at any site. (genscript.com)
  • Prices and turnaround times shown for Cat No. SC1441 apply to mutagenesis ordered at the same time as new gene synthesis orders. (genscript.com)
  • The fastest, and usually the most cost-effective, way to get the mutant DNA constructs you need is to order Express Mutagenesis bundled with gene synthesis using our online quotation form . (genscript.com)
  • For batch orders of gene synthesis bundled with mutagenesis, please download our Quick Quotation Request Form . (genscript.com)
  • Spontaneously arising and MMS-induced insertion/deletion mutations and large rearrangements were also more common in BRCA1/2 mutant cells compared with the wild-type control. (crick.ac.uk)
  • As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems. (biomedcentral.com)
  • Transitions and deletions accounted for 30 and 10% of the detected mutations, respectively. (cdc.gov)
  • Insertion-deletion (InDel) backbone mutagenesis of Anc(HLD-RLuc) challenged the scaffold dynamics. (muni.cz)
  • Systematic deletion mutagenesis of Tsp localized the binding site to amino acids 206-307, a region that completely encompasses the putative PDZ domain (217-301). (embl.de)
  • We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster . (biomedcentral.com)
  • Here we introduce a novel mutagenesis protocol utilizing the cross-linking drug hexamethylphosphoramide (HMPA) [ 24 ], streamlined fly genetics and high-throughput fragment analysis on sequencers to demonstrate the feasibility of our reverse genetics approach. (biomedcentral.com)
  • The vector pSelAct-KO is a recombination based, markerless knockout system that uses dual selection to engineer seamless deletions of a region of interest, providing opportunities for repeated higher-order genetic knockouts. (biorxiv.org)
  • For other HDR donor applications, including conditional knockout, mutagenesis, in-frame fusion tagging, and more, please contact us for a custom quote. (genecopoeia.com)
  • Chou Q (1992) Minimizing deletion mutagenesis artifact during Taq DNA polymerase PCR by E. coli SSB. (springer.com)
  • Our latest issue of NEB Expressions covers lyophilized assays for regulated markets, our new phi29-XT RCA Kit, web tool updates, and solutions for automating DNA assembly, sequencing, mutagenesis, cell-free protein expression and more! (neb.com)
  • An increased and prolonged gamma-H2AX signal in MMS-treated BRCA1/2 cells suggested an aberrant processing of stalled replication forks as the cause of increased mutagenesis. (crick.ac.uk)
  • The success of our approach suggests that a strategy comprising (i) constructing a stable and evolvable template, (ii) mapping functional regions by backbone mutagenesis, and (iii) transplantation of dynamic features, can lead to functionally innovative proteins. (muni.cz)
  • Deletion of either rnhB or rnhC triggers RNA:DNA hybrid accumulation, but with distinct patterns of mutagenesis and hybrid accumulation. (biorxiv.org)
  • Due to its long safety record, organisms obtained by physical and chemical mutagenesis are exempt from the provisions of the GMO legislation in the EU. (frontiersin.org)
  • A non-polar iglA deletion mutant was defective for growth in mouse macrophage-like cells, and in cis complementation largely restored the wild type macrophage growth phenotype. (biomedcentral.com)
  • Following its decision, the EU Court considers that the NBTs operate "at a rate out of all proportion to those resulting from the application of conventional methods of mutagenesis. (frontiersin.org)
  • The high rate of base substitution mutagenesis demonstrated by our experiments is likely to significantly contribute to the oncogenic effect of the inactivation of BRCA1 or BRCA2. (crick.ac.uk)
  • And in fact by some very tortuous reasoning which of course clarified the moment one said it, I said that, 'What would it be like if there were not only base substitutions but base additions and deletions? (webofstories.com)
  • Is the Subject Area "Deletion mutagenesis" applicable to this article? (plos.org)
  • Here, we present a suite of tools for genetic manipulation in the tomato pathogen C. michiganensis including a markerless deletion system, an integrative plasmid, and an R package for identification of permissive sites for plasmid integration. (biorxiv.org)
  • The NarI restriction site sequence 5′-G1G2CG3CX-3′ in Escherichia coli is a well-known mutational hotspot, in which lesioning of acetylaminofluorene (AAF) at G3* induces a greater −2 deletion frequency than that at other guanine sites. (jascoinc.com)
  • The working group prepared a list of seven new plant breeding techniques: zinc finger nuclease (ZFN) technology, oligonucleotide-directed mutagenesis (ODM), cisgenesis and intragenesis, grafting on GM-rootstock, RNA-dependent DNAmethylation, agro-infiltration "sensu stricto," and reverse breeding. (frontiersin.org)
  • This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus. (umassmed.edu)
  • We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
  • The latest version of NEBaseChanger® is available to help design site-directed mutagenesis primers. (neb.com)
  • GenScript's site-directed mutagenesis services provide a fast, cost-saving, way to get 100% accurate mutant DNA clones, without having to spend your time and money on mutagenesis kits to do PCR mutagenesis, cloning, and sequencing in your own lab. (genscript.com)
  • Site-directed mutagenesis of a surface residue at the peptide binding site of the PDZ domain, valine 229, to Glu or Gln resulted in an increase in the K(M) value but had no effect on the k(cat) value. (embl.de)
  • The toolkit created in Phase I will be expanded through the implementation of several new high throughput approaches toward yield improvement including mutagenesis and cDNA library screening and deletion of protease genes as well as traditional fermentation and purification process development. (sbir.gov)
  • For mutagenesis from a template previously synthesized by GenScript (Cat No. SC1626), please Quote with your previous order number to request a quote for price and turnaround time. (genscript.com)
  • Here the authors report a protein-engineering framework based on InDel mutagenesis and fragment transplantation resulting in greater catalysis and longer glow-type bioluminescence of the ancestral luciferase. (muni.cz)
  • 1,260 of these deletions turned out to be essential. (wikipedia.org)