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  • substrates
  • HCP exhibited cooperative inhibition of these enzymes since the observed maximum interaction coefficient, n', between HCP binding sites ranged between 1.62 and 3.33 but it was not an allosteric effector as evidenced by Hill coefficients for the substrates of approximately 1.0 both in the absence and the presence of HCP. (oregonstate.edu)
  • Other substrates may bind to the enzyme as well. (wikibooks.org)
  • The enzyme accepted a selection of vicinal diketones, α-hydroxy ketones and vicinal diols as alternative substrates. (megazyme.com)
  • The enzyme from pig liver can accept substrates with acyl chain lengths of 4 to 16 carbon atoms, but is most active with C8 to C12 compounds. (creative-enzymes.com)
  • NADP
  • One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP + at pH 7.4 and 25 o C. (megazyme.com)
  • Here we report the steady-state kinetics for 17beta-HSD1 at 37 degrees C and pH 7.5, using a homogeneous enzyme preparation with oestrone, dehydroepiandrosterone (DHEA) or dihydrotestosterone (DHT) as substrate and NADP(H) as the cofactor. (semanticscholar.org)
  • Regulation
  • Mark R. Garbrecht and Thomas J. Schmidt, "Expression and Regulation of 11- β Hydroxysteroid Dehydrogenase Type 2 Enzyme Activity in the Glucocorticoid-Sensitive CEM-C7 Human Leukemic Cell Line," ISRN Oncology , vol. 2013, Article ID 245246, 5 pages, 2013. (hindawi.com)
  • In this study, we determined the correlation between the effects of Ps leaf water extract with the regulation of 11β-hydroxysteroid dehydrogenase (HSD) type 1 enzyme activity in serum and bone of glucocorticoid-induced osteoporotic rats. (mdpi.com)
  • Human oestrogenic 17beta-hydroxysteroid dehydrogenase specificity: enzyme regulation through an NADPH-dependent substrate inhibition towards the highly specific oestrone reduction. (semanticscholar.org)
  • substrate
  • Another substrate is the dihydrolipoyl group in the H-protein of the glycine-cleavage system (click here for diagram), in which it acts, together with EC 1.4.4.2, glycine dehydrogenase (decarboxylating), and EC 2.1.2.10, aminomethyltransferase, to break down glycine. (creative-enzymes.com)
  • Furthermore, we identified a substrate for the enzyme in cauliflower extract, which co-eluted with L-galactono-gamma-lactone by high-performance liquid chromatography, suggesting that this compound is a naturally occurring precursor of L-ascorbic acid biosynthesis in vivo. (ugent.be)
  • glucose-6-phospha
  • The enzyme converts glucose-6-phosphate to ribulose-5-phosphate in the pentose phosphate pathway. (sciencephoto.com)
  • High purity Hexokinase (yeast) / Glucose-6-phosphate dehydrogenase (G6P-DH) ( Leuconostoc mesenteroides) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • Fusions of the first two enzymes in the pentose phosphate pathway, glucose-6-phosphate dehydrogenase G6PD and 6-phosphogluconolactonase 6PGL, have been previously described in two distant clades, chordates and species of the malarial parasite Plasmodium. (duhnnae.com)
  • yeast
  • The results from these studies suggest that the mechamism for inhibition of dehydrogenases by HCP and related compounds is quite complex, probably involving, at least in the case of torula yeast G6PD: the direct binding of HCP to the enzyme, resulting in inhibition and conformational changes in the enzyme and dissociation of the active dimeric form of the enzyme to its inactive subunits. (oregonstate.edu)
  • mitochondrial
  • Besides a crucial role in patients that suffer from pyruvate dehydrogenase deficiency -one of the most common neurodegenerative disorders associated with abnormal mitochondrial metabolism- PDH is also topic of interest of many studies on cancer . (axonmedchem.com)
  • activity
  • This study was performed to investigate, if pyruvate dehydrogenase enzyme (PDH) is affected in its activity giving further hints for a derangement of oxidative metabolism. (frontiersin.org)
  • Calculate the enzyme activity as follows with decrease in absorbance for one min. (biocyclopedia.com)
  • Previous studies show that deficiency in the activity of this enzyme can lead to incomplete metabolism of fluoroprimidine derivatives and severe complications. (radioncology.com)
  • botrytis) to apparent homogeneity with an overall yield of 1.1%, The purification procedure consisted of anion exchange, hydrophobic interaction, gel filtration, and fast protein liquid chromatography, The enzyme had a molecular mass of 56 kDa estimated by gel filtration chromatography and SDS-polyacrylamide gel electrophoresis and showed a pH optimum for activity between pH 8.0 and 8.5, with an apparent K-m of 3.3 mM for L-galactono-gamma-lactone. (ugent.be)
  • Likewise, racemic acetoin was reduced with a specific activity of up to 115 U/mg yielding a mixture of ( R , R )- and meso -butane-2,3-diol, while the enzyme reduced butane-2,3-dione ( V max 74 U/mg) solely to ( R , R )-butane-2,3-diol via ( R )-acetoin. (megazyme.com)
  • G6PD
  • Surprisingly, our search revealed two additional fusion events, one that combined 6PGL and G6PD in an ancestor of the protozoan parasites Trichomonas and Giardia, and another fusing G6PD with phosphogluconate dehydrogenase 6PGD in a species of diatoms. (duhnnae.com)
  • oxidation
  • Dehydrogenases catalyze the oxidation of alcohols to carbonyl compounds by using either NAD+ or NADP+. (wikibooks.org)
  • The isolated enzyme catalyzed the ( R )-specific oxidation of ( R , R )- and meso -butane-2,3-diol to ( R )- and ( S )-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. (megazyme.com)
  • extract
  • Determine the protein content by the method of Lowry et at in the enzyme extract. (biocyclopedia.com)
  • This suggests that Ps leaf water extract was able to prevent bone loss due to long-term glucocorticoid therapy by acting locally on the bone cells by increasing the dehydrogenase action of 11β-HSD type 1. (mdpi.com)
  • serum
  • The serum concentration of DPD enzyme was measured by the enzyme-linked immunosorbent assay (ELISA) kit and its relationship with incidence of capecitabine induced side effects was evaluated. (radioncology.com)
  • protein
  • The amino acid sequence of the encoded protein is only distantly related to previously studied enzymes (identity 33-43%) and exhibited some uncharted peculiarities. (megazyme.com)
  • conversion
  • The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. (activemotif.jp)