Degrees proteins chromatography gel. Medical search. Frequent questions

One of these peaks (III) was successfully rechromatographed by ion-exchange chromatography and further purified by HPLC using a gel-permeation column. (nih.gov)
SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase high-performance liquid chromatography (RP-HPLC) analysis showed that most macromolecular proteins in tempeh had been degraded during fermentation and more of the small peptides were released from black bean tempeh during digestion, respectively. (bvsalud.org)
The Primus instrument is a fully automated glycohemoglobin analyzer, which utilizes the principle of boronate affinity high performance liquid chromatography (HPLC) 4 . (cdc.gov)
Characterisation of polymer-drug conjugates and targeted nanotherapeutics by High-performance Liquid Chromatography (HPLC), Gel Permeation Chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), Nuclear Magnetic Resonance (NMR), Dynamic Light Scattering/ Electrophoretic Light Scattering (DLS/ELS), and microscopy (electron microscopy and confocal microscopy). (ibecbarcelona.eu)
The F2 peak was separated on reverse-phase column by high performance liquid chromatography (HPLC) and ACE inhibitory activity was measured. (jejunu.ac.kr)
Plasma amino acids were measured using high-performance liquid chromatography (HPLC), while, serum ammonia and oxidative stress markers were measured using colorimetric methods for all included children. (dovepress.com)
Analyze the PEGylated product to confirm its identity, purity and degree of PEGylation, using thetechniques such as mass spectrometry, SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) and HPLC (High-Performance Liquid Chromatography) can be used for characterization. (ebclink.com)
1985) and in rat urine by high-performance liquid chromatography (HPLC) with ultraviolet (UV) absorbance detection following formation of the 2,4-dinitrophenylhydrazone derivative (Shara et al. (cdc.gov)

Brain calmodulin was purified by affinity chromatography on fluphenazine-Sepharose. (erowid.org)
Tryptophan hydroxylase prepared by affinity chromatography and free of calmodulin was not activated by ATP-Mg2+ but if calmodulin was then added to the enzyme, the latter was reactivated byATP-Mg2+ providing Ca2+ was present. (erowid.org)
Further purification of this complex by DEAE A-25 column chromatography disclosed the presence of five peaks with varying degrees of affinity for the ion-exchange resin. (nih.gov)
From the resulting darkbrown fluid the proteolytic fractions were purified by gel-filtration, anion exchange chromatography and affinity chromatography to a high degree. (wikipedia.org)
1984) Purification of the messenger RNA cap-binding protein using a new affinity medium. (jenabioscience.com)
Affinity chromatography is the preferred method of bioselective adsorption and subsequent recovery of a compound from an immobilized ligand. (sigmaaldrich.com)
The protein Avidin has an extremely high affinity for the cofactor Biotin (Kdiss 10-15M). (sigmaaldrich.com)
Dye ligand chromatography is affinity chromatography that utilizes covalently bond textile dye (reactive dyes) to purify proteins. (sigmaaldrich.com)
This differs from techniques such as ion exchange chromatography (IEX) or affinity chromatography (AC). (pharmaceuticalonline.com)
The bioavailability of lead in kidney is mediated in part by binding to endogenous high-affinity cytosolic lead-binding proteins (PbBP), which are not detectable in liver. (nih.gov)
In the present study, lectin was extracted and purified from the leaves of Indian borage by affinity chromatography using Seralose-4B beads that were coupled with dextrose sugar. (alliedacademies.org)
31] AFFINITY CHROMATOGRAPHY 345 [31] Affinity Chromatography By PEDRO CUATRECASAS AND CHRISTIAN B. ANFINSEN General Considerations Conventional procedures of protein purification are generally based on relatively small differences in the physicochemical properties of the proteins in the mixture. (nih.gov)
The selective isolation and purification of enzymes and other biologically important macromolecules by "affinity chromatography" exploits the unique biological property of these pro- teins or polypeptides to bind ligands specifically and reversibly. (nih.gov)
Affinity chro- matography, as summarized in the present article, exploits the phenom- enon of specific biological interaction in a large variety of protein- ligand systems. (nih.gov)
Proteins not exhibiting appreciable affinity for the ligand will pass unretarded through the column, whereas those which recognize the inhibitor will be retarded in proportion to the affinity existing under the experimental conditions. (nih.gov)
Affinity chromatography may also be useful in concentrating dilute solutions of proteins, in removing denatured forms of a purified protein, and in the separation and resolution of protein components resulting from specific chemical modifications of purified proteins. (nih.gov)
The following general considerations should serve as guidelines in the preparation of adsorbents for affinity chromatography. (nih.gov)
4) These chemical groups should be abundant in order to allow attainment of a high effective concentration of coupled inhibitor (capacity), so that satisfactory adsorption can be obtained even with protein-inhibitor systems of low affinity. (nih.gov)
These diol groups provide the basis for separation of glycated and non-glycated components by boronate affinity chromatography 1,2,3 . (cdc.gov)
The detergent-solubilized membrane fraction was loaded onto a column packed with the TALON sorbent for metal affinity chromatography equilibrated with buffer A with 0.02% DDM, and the column was washed with the same buffer. (springer.com)
In this study AaGroEL protein has been purified by using ATP Affinity chromatography and electroelution methods. (iyte.edu.tr)

Proteins were separated by electrophoresis in NaDodSO4/ polyacrylamide gel slabs. (erowid.org)
[ 40 ] The huPON1 sample applied here had a specific arylesterase activity of ~1000 U/mg (1 U = 1 µmol of phenyl acetate hydrolyzed per minute per mg protein at 1 mM substrate concentration), as previously reported, [ 41 ] and SDS-polyacrylamide gel electrophoresis indicated a single band (= 90% protein purity). (medscape.com)
[ 35 ] The homogeneity of the preparations was assessed by non-denaturing gradient gel electrophoresis (4-20% polyacrylamide, Pharmacia) and electron microscopy indicating the formation of particles of ~10 nm. (medscape.com)
Screening involved testing for common variants, then protein truncation testing of long exons, and then denaturing gradient gel electrophoresis or denaturing high-performance liquid chromatography for the remainder of BRCA1 and BRCA2, respectively. (nih.gov)
The purified protein showed only one major band, MW 14,000, by SDS polyacrylamide gel electrophoresis. (usu.edu)
If a forum of layers of Commercial proteins is outdated at the body of the high-density and follows electrophoresed by the immunochemistry of polar through the medium, differences above the editor assay electrophoresis will identify between the community roles and influence from the aggregation of the force most only. (sweetlilyspa.com)
The latter includes 2-D gel electrophoresis, high performance liquid chromatography, membrane ultrafiltration, atomic force microscopy, and capillary electrophoresis. (studyqa.com)
nautica cells to homogeneity as judged by polyacrylamide gel electrophoresis. (unl.pt)

Size exclusion chromatography (SEC), also known as gel filtration, is the mildest of all the chromatography techniques. (pharmaceuticalonline.com)
Gel permeation chromatography, also known as size exclusion chromatography (GPC/SEC), is heavily relied upon for its ability to measure properties, such as molecular weight, that define the performance of polymers, macromolecules and proteins. (laboratorytalk.com)
Its is typically achieved through techniques such as size-exclusion chromatography, ultrafiltration or dialysis. (ebclink.com)

Suitable for purification of eukaryotic mRNA cap-binding proteins. (jenabioscience.com)
Each is designed for highly specific and efficient purification of proteins and related compounds. (sigmaaldrich.com)
Immobilized sugars and sugar derivatives offer an effective means of purification for a variety of proteins, which recognize and bind carbohydrate moieties. (sigmaaldrich.com)
Nonetheless, tag-free NoV protein purification schemes have hardly been reported because of low isoelectric degree of NoV proteins, which ends up in extraordinarily aggressive binding between the aim protein and yeast host cell proteins at alkaline pH. (aabioetica.org)
On this analysis, a two-step purification scheme based totally on flooring histidines and the fee on the NoV GII.4 stress P protein was developed. (aabioetica.org)
In- herent advantages of this method of purification are the rapidity and ease of a potentially single-step procedure, the rapid separation of the protein to be purified from inhibitors and destructive contaminants, e.g., proteases, and protection from denaturation during purification by active site ligand-stabilization of protein tertiary structure. (nih.gov)
The specific conditions for purification of a particular protein must be highly individualized since they will reflect the uniquely specific biological property of selective interaction with a given ligand. (nih.gov)
We have performed heterologous expression and purification of Neonothopanus nambi luciferase, obtained a protein suitable for subsequent crystallization, and also determined some biochemical properties of the recombinant luciferase. (springer.com)
The isolation, purification, and partial characterization of a novel iron-containing protein from the sulfate-reducing anaerobic bacterium, Desulfovibrio gigas , is described. (usu.edu)
Protein expression and purification. (ibecbarcelona.eu)
After the purification step lps contamination has been removed by detoxi-gel endotoxin removal gel and detected by LAL Assay. (iyte.edu.tr)
The development of the purification procedure thus provides an important step for subsequent study of microtubule-associated protein-tubulin and plasma-membrane-microtubule cytoskeleton interactions of trypanosomes, and increases the potential for development of tubulin-based anti-trypanosome drugs. (ox.ac.uk)
Biochemical and biomedical engineering laboratories contain a range of equipment for cell culture and fermentation, and for protein purification, analysis, and characterization. (studyqa.com)
Lectures dealing with methods for theoretical modelling of membrane protein structure, fusion protein techniques, X-ray crystallography, heterologous expression, solubilisation and purification of membrane proteins are also included in the course. (lu.se)
In silico exercise addressing potential problems concerning the detection of heterologously expressed membrane proteins, solubilisation and evaluation of detergent properties, ion exchange chromatography and gel filtering in the presence of a detergent, and control of the protein's stability and integrity after purification. (lu.se)

Liquid chromatography combined with mass spectrometry is a widely adopted means of studying proteins and has been the focus of much innovation in the past few years. (genengnews.com)
These include redesigned mass spectrometry instrumentation combined with stable isotopic labeling and novel 2-D gel technologies fused with new methods of differential staining. (genengnews.com)
Performed headspace gas chromatography mass spectrometry (quality control of water). (livecareer.com)
Formaldehyde has been measured in blood by gas chromatography (GC) in conjunction with mass spectrometry (MS) after derivatization of the formaldehyde to the pentafluorophenylhydrazone (Heck et al. (cdc.gov)

This effect results in part from a marked increase in binding of 203Pb to the PbBP relative to control liver cytosol (no PbBP) as demonstrated by Sephadex G-150 gel filtration chromatography. (nih.gov)
Equally, the NoV capsid protein VP1 was moreover purified using HisTrap and gel filtration chromatography based totally on native flooring histidines and self-assembly potential, with 20% restoration and over 90% purity. (aabioetica.org)
The fractions with the highest luminescence activity were loaded onto a column with Sephacryl S-300 in 50 mM Na-phosphate buffer (pH 7.0) containing 150 mM NaCl and 0.04% DDM for gel filtration. (springer.com)
Next, below 5 kDa molecular weight fraction was separated on Sephadex G-25 gel filtration chromatography and ACE inhibitory activity was measured. (jejunu.ac.kr)
Trypanosome tubulin was purified to near homogeneity by chromatography on DEAE-Sephadex, Amicon filtration and assembly-disassembly in vitro. (ox.ac.uk)

The pI range analyzed in this study is comparable to that reported using strong cation exchange chromatography. (nih.gov)
However, the degree of pI overlapping in CIEF fractions is drastically lower than that in strong cation exchange chromatography using a salt gradient at acidic pH. (nih.gov)
What charge exists on the column in cation exchange chromatography? (flashcardmachine.com)

Waters introduced the Protein Expression System, which substitutes microcapillary liquid chromatography for 2-D gel separation, with a direct connection to the mass spectrometer. (genengnews.com)
The peptide formation was investigated by high-performance liquid chromatography. (springeropen.com)

She became well-versed in cutting-edge chemical analysis techniques, including paper chromatography, which she demonstrates in the photo below. (nih.gov)
She became well versed in cutting-edge chemical analysis, like paper chromatography, a technique she demonstrated in the photo. (nih.gov)

Sephadex G-75 column chromatography of the kidney cytosol showed that most of the Cd and Zn were bound to a protein complex with an estimated molecular mass of 45,000 daltons. (nih.gov)

We observed that the soluble protein content of digested black bean tempeh was generally significantly higher than that of digested unfermented black beans at the same digestion stage (P (bvsalud.org)
However it has proved challenging to identify a heterologous expression platform that can produce a soluble protein-based vaccine in a manner compliant with current Good Manufacturing Practice (cGMP). (nature.com)
This work identifies Drosophila S2 cells as a clinically-relevant platform suited for the production of 'difficult-to-make' proteins from Plasmodium parasites, and identifies a PfRH5 sequence variant that can be used for clinical production of a non-glycosylated, soluble full-length protein vaccine immunogen. (nature.com)
A total of 1156 unique proteins and 1866 unique peptides are identified in the soluble fraction of yeast cell lysates. (nih.gov)
By using only the soluble fraction, our studies already demonstrate the capabilities of CIEF-based multidimensional separation technology for identifying a larger number of soluble yeast proteins than other techniques reported in the literature. (nih.gov)
When is a protein most/least soluble in solution with its pI relative to the pH? (flashcardmachine.com)

You sample the same proteins over and over again, so the peptides you detect are overrepresented, resulting in an inability to detect peptides of interest-a situation we refer to as undersampling. (genengnews.com)
The purpose of the present study was to evaluate the antagonistic effect of corn protein hydrolysate with glutamine-rich peptides on ulcerative colitis. (bvsalud.org)
Most importantly, the amount of tryptic peptides employed for performing yeast proteome analysis is only around 960 ng which is two to three orders of magnitude less than those utilized in the current non-gel based proteome techniques. (nih.gov)
In an activated form, they hydrolyze the protein substrate at specific sites and the released peptides can be absorbed. (springeropen.com)
The entirety of produced protein fragments and peptides is called proteolysome (Pimenta and Lebrun 2007 ). (springeropen.com)

Molecules are separated by size differences as they pass through a resin packed in a column, and molecules do not bind to the chromatography resin, meaning buffer composition does not directly affect resolution, which is the degree of separation between peaks. (pharmaceuticalonline.com)
But this size measurement only works for large molecules and not for small species like proteins. (laboratorytalk.com)
This approach involves tagging the biomolecules of interest with fluorescent molecules, such as green fluorescent protein (GFP), so that they can be identified in cells. (elifesciences.org)
proteins), followed by further GO and KEGG pathway analyses of these overlapped molecules, and molecular network analysis to obtain the hub molecules with Cytoscape. (frontiersin.org)
Protein and small molecules analysis. (massey.ac.nz)
PEGylation is a process of both covalent and non-covalent attachment or consolidation of polyethylene glycol (PEG) polymer chains to molecules and macrostructures, such as drugs, therapeutic proteins or vesicles. (ebclink.com)

Recent advances in high-throughput technologies in genomics, proteomics, and metabolomics, in combination with robust bioinformatics enable researchers to rapidly survey thousands of differentially expressed genes, proteins, or metabolites simultaneously, thus employing an integrative approach to identify unique biomarker signatures. (nih.gov)

Mark McDowall, Ph.D., strategic development manager, MS, and James Langridge, Ph.D., senior manager proteomics business, of Waters (www.waters.com), say the characterization of serum and plasma is particularly fraught with peril given the vast span (a factor of 1011) over which protein concentrations range. (genengnews.com)
Isolation and partial characterization of a high molecular weight Cd/Zn-binding protein from the kidney of the scallop Placopecten magellanicus: preliminary studies. (nih.gov)
The lack of homology with other enzymes does not allow modeling the spatial structure of this protein, and the difficulty of its isolation from a natural source prevents obtaining a sufficient amount of this protein for crystallization and characterization of its properties. (springer.com)

The enzyme was purified from pooled blood sera of healthy individuals by three sequential gel chromatographic steps: Cibacron Blue 3GA, DEAE I, and DEAE II as described. (medscape.com)
The proteins are synthesised with N-terminal signal and pro-enzyme sequences, and many contain multiple domains C-terminal to the protease domain. (wikipedia.org)
In this study, black bean tempeh and unfermented black beans were digested in vitro and changes in protein degradation, phenolic compound release, angiotensin I-converting enzyme (ACE)-inhibitory activity, and antioxidant activity between the two groups were compared. (bvsalud.org)
The proenzymes are enzymatically inactive proteins which are converted to proteolytic enzymes by the action of an "activator", generally another proteolytic enzyme produced at an earlier stage in the coagulation cascade. (justia.com)
The enzyme modulators are principally cofactors such as calcium ions or nonenzyme proteins and most are essential if the enzymes to exhibit any catalytic activity at all. (justia.com)
Here, however, we show that some group 1 coronavirus spike proteins carry a furin enzyme recognition motif and can actually be cleaved, as demonstrated for a feline coronavirus. (srcinhibitors.com)
The enzyme specific peptide fingerprint produced with this proteolytic capillary reactor system correlated with the degree of hydrolysis, which can be controlled over the residence time by adjusting the flow rate. (springeropen.com)
2012 ). When using enzymes for digesting food proteins, an enzyme specific peptide composition, the so-called peptide fingerprint is formed. (springeropen.com)
In industrial processes, protein hydrolysis is often carried out in batch processes and is mostly used in fermentation and enzyme based processes, like brewing, cheese manufacturing, meat tenderization or baking (Godfrey and Reichelt 1982 ). (springeropen.com)

1. The proteins encoded by these genes are classified as alpha or beta forms on the basis of the predicted amino acid sequences and have homologs in man. (everypatent.com)

A family of proteins called p21-activated kinases (PAKs) has been implicated in autism and other disorders like Huntingtin disease and Alzheimer disease, but it is not fully understood how these proteins interact with endocannabinoid. (elifesciences.org)

A solution containing the macromolecule to be purified is passed through a column containing an insoluble polymer or gel to which a specific competitive inhibitor or other ligand has been covalently attached. (nih.gov)

Therefore, for further experiments, we decided to use the yeast strain, producing luciferase with a sequence of six histidine residues at the C-terminus of the protein. (springer.com)
Furthermore, the distribution of codon adaptation index value for identified yeast proteins approximates to that predicted for the entire yeast proteome. (nih.gov)

KEGG pathway analysis of 6076 proteins obtained 114 statistically significant pathways, including endocytosis, and spliceosome signaling pathways. (frontiersin.org)
By combining the strength of our proteome technologies with the knowledge in Drosophila genetics, the results gathered in this study provide valuable information toward the difference between autophagy and apoptosis and the novel protein signaling pathways that mediate steroid-triggered cell death. (nih.gov)

Historically, the use of cataplasms was shown to induce allergy to mustard, documenting the reality of sensitization to food proteins by skin application. (manchester.ac.uk)
Common food proteins like casein, sunflower and lupin protein isolates were tested for continuous hydrolysis in the developed reactor system. (springeropen.com)
Food proteins are important macronutrients providing the human body with essential amino acids. (springeropen.com)

The amino acid composition was similar to that of a number of membrane proteins. (usu.edu)
Membrane proteins play a key role in the cell's energy metabolism and in its signalling and communication with its environment. (lu.se)
More than half of all drugs that are in use today target membrane proteins. (lu.se)
The main aim of the course is to enable students to acquire specialised knowledge and understanding of membrane biochemistry and the molecular structure, topology and functional mechanisms of membrane proteins. (lu.se)
The lectures address the three different main types of membrane proteins and associated cellular processes: transport and transporters, signal transduction and receptors, bioenergetics and photosynthetic and respiratory proteins. (lu.se)
Group discussions about e.g. the similarities/dissimilarities, cloning and overexpression strategies, and structure and function of membrane proteins. (lu.se)
The project entails practice in literature searching, project planning and documentation, and provides specialised practical knowledge of expression and management of membrane proteins. (lu.se)

These results indicate that activity-dependent CK2 phosphorylation of the NR2B PDZ ligand regulates the interaction of NMDAR with PSD-95/SAP90 family proteins as well as surface NMDAR expression and may be a critical mechanism for modulating excitatory synaptic function and plasticity. (jneurosci.org)

The heterologous P protein expressed by Pichia pastoris self-assembles into P particles. (aabioetica.org)

Lectins are the proteins that have the ability to bind to sugar moieties and agglutinate cells. (sigmaaldrich.com)
Lectins are heterogeneous proteins of non-immune source and reversibly bind to carbohydrates having more than one non- catalytic domain, resulting in cell agglutination. (alliedacademies.org)
In addition, the CH domain occurs also in a number of proteins not known to bind actin, a notable example being the vav protooncogene. (embl.de)

As they explained, the classic separation of complex protein mixtures in large proteomic studies was most commonly carried out using 2D-PAGE. (genengnews.com)

Lectins are glycan-binding proteins that have been of interest to researchers because of their multi-physiological role. (alliedacademies.org)

For preparative extraction, we used DDM at a concentration of 10 mM, due to its availability and good applicability for chromatography with UV detection. (springer.com)
0 is scattering at zero degrees to the incident beam, K is a constant, c is concentration and Mw is weight average molecular weight. (laboratorytalk.com)
What occurs to proteins in a column when the salt concentration is increased? (flashcardmachine.com)

Whether or Not a infected Imaginary ebook can set between acids is on the full point of the username and on the cycle proteins of the isolation. (sweetlilyspa.com)

In contrast, the second route of appearance for the inhibitors is not believed to be a function of the administration of therapeutic blood protein fractions. (justia.com)

If you are any reagents or therapeutic proteins, Try that time for Program. (sweetlilyspa.com)
PEGylation process involves the attachment of PEG chains to a molecule of interest, such as a therapeutic protein or drug. (ebclink.com)

Overall, the False Discovery Rate approach allows one to strike a balance between the need to find statistically valid proteins of interest and the additional cost that is associated with following up on false positives. (decodon.com)

If you look in the literature, there are a number of groups doing peptide analysis of serum proteins in which the results are biased toward the high-abundance proteins," states Dr. McDowall. (genengnews.com)

It was noted by the authors that this method was free from interferences from proteins and bacterial cells so it might have applicability to biological fluids such as blood or urine. (cdc.gov)

The key thing to notice is that it relates molecular weight very specifically to the value of scattering at zero degrees to the incident beam. (laboratorytalk.com)
The majority of proteins fall into this category along with some other sample types like low molecular weight polymers, and here RALS is a sound choice. (laboratorytalk.com)
64kDa GroEL protein which is a molecular chaperone and a heat shock protein can be the potential candidate for being a virulence factor. (iyte.edu.tr)
Interactions between NMDA receptors (NMDARs) and the PDZ [postsynaptic density-95 (PSD-95)/Discs large/zona occludens-1] domains of PSD-95/SAP90 (synapse-associated protein with a molecular weight of 90 kDa) family proteins play important roles in the synaptic targeting and signaling of NMDARs. (jneurosci.org)
The purified protein is a heterodimer with subunits of molecular masses of 54 and 18 kDa. (unl.pt)
120 credits) in Chemistry and Molecular Biology and compulsory for a degree of Master of Science (120 credits) in Protein Science. (lu.se)
A number of proteins from each process, for which the structure is known, are explored in greater detail in order to highlight the functional molecular mechanisms. (lu.se)

Metal analysis of the purified protein showed that it contained Cd, Zn, and Cu in ratios of 1:1:1. (nih.gov)

The antibodies induced by one protein variant were shown to be qualitatively similar to responses induced by other vaccine platforms. (nature.com)

This allows identification of the peptide ions with a high degree of quantitative and qualitative accuracy, according to the company. (genengnews.com)
Protein identification is then achieved via database search using the exact mass of the tryptic peptide and fragment ion information. (genengnews.com)
Choose the molecule you would liketo PEGylate, such as a protein, peptide, small molecule drug or other biologically active compound. (ebclink.com)

This is followed by experimental determination using genetic construction and expression of a fusion protein of the membrane protein and a marker protein in a bacterial system which is subsequently analysed. (lu.se)

Polymerization of the tubulin in vitro yielded long, structurally normal, microtubules and some sheet structures on addition of GTP and incubation at 37 degrees C, in either the presence or the absence of Mg2+. (ox.ac.uk)

Much more complex than genomics, it represents the industrial-scale analysis of the vast array of proteins in their myriad configurations. (genengnews.com)
A number of scientists at academic and commercial institutions have complained of poor experimental design, use of inappropriate statistical analysis, and erroneous protein identification, which appeared in the published proteomics database. (genengnews.com)
But in really complex protein mixtures, such as a tumor cell lysate, there maybe several thousand individual proteins and 2-D gel analysis would be overwhelmed due to the complexity and dynamic range. (genengnews.com)
2016) Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions. (jenabioscience.com)
Cox regression analysis was used to examine cancer outcomes reported by the case probands for their 8680 first-degree relatives. (nih.gov)
The results of elemental analysis of a crude protein preparation were 5.3% iron, 2.9% sulfur and 11 .9% nitrogen. (usu.edu)
Immunological analysis of the purified tubulin revealed tyrosinated and acetylated alpha-tubulin, in addition to defining the migration characteristics of the alpha- and beta-tubulin on one-dimensional SDS/polyacrylamide gels. (ox.ac.uk)

2017) p90RSK Blockade Inhibits Dual BRAF and MEK Inhibitor-Resistant Melanoma by Targeting Protein Synthesis. (jenabioscience.com)

Instead PAK1 boosts levels of another protein called COX-2 and reduces levels of a molecule called anandamide, which together restrict endocannabinoid's inhibitory effects. (elifesciences.org)

On the basis of its primary structure one proteolytic fraction from Astacus obviously represented an unknown protein and was named Astacin. (wikipedia.org)

Hydrolysis is conducted by an enzymatic or acidic breakdown of proteins (Tsugita and Scheffler 1982 ). (springeropen.com)

The resultant protein peak which was resistant to disaggregation by 20 mM dithiothreitol gave a preliminary amino acid composition with cysteine, glycine, alanine, and lysine as the major amino acids. (nih.gov)
Results of these studies indicate that scallop kidney produces a protein complex which appears to share both similarities with mammalian metallothionein with respect to the presence of both Cd and Zn but different with respect to apparent size, amino acid composition, and ultraviolet absorption spectrum. (nih.gov)
A number of actin-binding proteins, including spectrin, alpha-actinin and fimbrin, contain a 250 amino acid stretch called the actin binding domain (ABD). (embl.de)
Proteins containing only a single amino terminal CH domain. (embl.de)

The specifically adsorbed protein can be eluted by altering the composition of the solvent so that dissociation oc- curs. (nih.gov)

This protein was highly expressed in prostate tumor tissues 7 and has a number of physiological functions, including serving as a cofactor for human immunodeficiency virus replication through an interaction with viral integrase 8 . (jrheum.org)
Totally 6076 proteins in NFPA tissues were identified, and 3598 DEGs between NFPA and control tissues were identified from GEO database. (frontiersin.org)

For food protein hydrolysates, the substrate and enzymes are mixed in huge, tempered tanks, and require continuous stirring, as well as a constant temperature and pH level for several hours. (springeropen.com)

An ideal insoluble support should possess the following properties: (1) It must interact very weakly with proteins in general, to minimize the nonspecific adsorption of proteins. (nih.gov)
The highly insoluble protein was isolated from the cell debris following osmotic shock of the bacteria. (usu.edu)

Their functions include activation of growth factors, degradation of polypeptides, and processing of extracellular proteins. (wikipedia.org)

GO and KEGG enrichment analyses were used to analyze the identified proteins. (frontiersin.org)

Studies of zinc release and/or displacement from PbBP under ALAD assay conditions (37 degrees C, + glutathione, pH 6.8) were conducted utilizing Sephadex G-25 chromatography. (nih.gov)

METHODS: Cultured renal tubular epithelial cells (LLC-PK1) were stored in University of Wisconsin (UW) or Euro-Collins (EC) solution at 4 degrees C for 20 hours. (cryonet.org)
Clustering methods can group expression profiles and gel images by similarity. (decodon.com)
Determination of the transmembrane topology of a protein starts with a model of the protein based on sequence information and theoretical methods. (lu.se)

Dystrophin protein is integral to the structural stability of the myofiber. (medscape.com)
Actin binding domains present in duplicate at the N-termini of spectrin-like proteins (including dystrophin, alpha-actinin). (embl.de)

The analytical column contains aminophenylboronic acid bonded to a porous polymer support (gel). (cdc.gov)

Anatomy14

Organisms13

Chemicals and Drugs99

Analytical, Diagnostic and Therapeutic Techniques and Equipment80

Phenomena and Processes50

Disciplines and Occupations6

Technology, Industry, Agriculture5

Information Science4

Health Care6

HPLC8

Affinity21

Electrophoresis8

Size Exclusion Chro3

Purification15

Mass spectrometry4

Filtration5

Exchange chromatography3

Liquid chromatography2

Paper chromatography2

Column chromatography1

Soluble6

Peptides5

Molecules6

Differentially expressed1

Characterization3

Enzyme9

Genes1

Family of proteins1

Covalently1

Yeast2

Pathways2

Food proteins3

Membrane proteins7

Ligand1

Heterologous1

Bind3

Separation1

Lectins1

Concentration3

Isolation1

Fractions1

Therapeutic2

Statistically1

Serum1

Applicability1

Molecular7

Purified protein showed1

Antibodies1

Peptide3

Bacterial1

Microtubules1

Analysis7

Synthesis1

Molecule1

Proteolytic1

Hydrolysis1

Amino4

Composition1

Tissues2

Substrate1

Insoluble2

Polypeptides1

Analyze1

Assay1

Methods3

Dystrophin2

Polymer1