Degradation pathways monitor protein. Medical search. Frequent questions

Redistribution assay technology relies on the imaging and quantification of intracellular protein translocation. (genengnews.com)
Bortezomib is a selective 26S proteosome inhibitor that functions by disrupting the major intracellular protein degradation pathway. (oncnursingnews.com)
Activation of the Yap transcription co-activator was monitored using a high content, image based assay that measured the intracellular localization of native YAP protein. (selectbiosciences.com)
such as components of the nuclear pore, by which YAP cytoplasmic/nuclear shuttling occurs, or how proteasomal degradation regulates intracellular YAP concentrations, which then alter YAP localization and transcription. (selectbiosciences.com)
Several PAR-binding modules orchestrate the relocation of DDR-associated factors in addition to the accumulation of intrinsically disordered proteins through an intracellular liquid demixing mechanism 11 , 12 . (nature.com)
The studies also showed that selective protein degradation occurs during this process and that intracellular cysteine proteases called caspases contribute to this degradation. (mayo.edu)
This pathway is regulated by a family of proteins called BCL2 family members that monitor the intracellular environment and regulate the integrity of mitochondria. (mayo.edu)
Albumin and IgG have remarkably long serum half-lives due to pH-dependent FcRn-mediated cellular recycling that rescues both ligands from intracellular degradation. (nature.com)
Thus, HERA is a novel cellular assay that can be used to predict how FcRn-binding proteins are rescued from intracellular degradation. (nature.com)
Autophagy is a cellular catabolic process that eliminates damaged cell organelles, unfolded proteins, and various intracellular pathogens through lysosomal degradation. (hindawi.com)
In general, autophagy degrades long-lived damaged intracellular proteins, in contrast to the ubiquitin-proteasome system, which controls the degradation of short-lived proteins [ 2 ]. (hindawi.com)

However, the proteasomal degradation takes place in the cytoplasm. (wikipedia.org)
Cysteine-specific ubiquitination protects the peroxisomal import receptor Pex5p against proteasomal degradation. (ruhr-uni-bochum.de)
Deubiquitination is the functionally reciprocal process of ubiquitination that prevents protein proteasomal degradation through deubiquitinating enzymes (DUB)-mediated removal of ubiquitin chains from target proteins [ 28 ]. (biomedcentral.com)

2023). Identification of potential selective autophagy receptors from protein-content profiling of autophagosomes. (unimedizin-mainz.de)
Autophagy stimulus-dependent role of the small GTPase Ras2 in peroxisome degradation. (ruhr-uni-bochum.de)
Vac8 controls vacuolar membrane dynamics during different autophagy pathways in Saccharomyces cerevisiae. (ruhr-uni-bochum.de)
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). (ruhr-uni-bochum.de)
Autophagy is a conserved catabolic process that eliminates dysfunctional cytosolic biomolecules through vacuole-mediated sequestration and lysosomal degradation. (hindawi.com)
Due to the significance of impaired autophagy in these diseases, there is increased interest in studying pathways and targets involved in maintaining efficient autophagic functions as potential therapeutic targets. (hindawi.com)
Induction of canonical autophagy is mainly controlled by the AKT/mTOR and AMPK signaling pathways and relies on the activation and assembly of two macromolecular complexes, the ATG1/ULK1 and the Class III phosphatidylinositol 3-kinase (PI3K) complex (Figure 1 ) [ 5 ]. (hindawi.com)
The autophagophore membrane then elongates and encloses the molecules to be degraded forming an autophagosome, which occurs in two separate conjugation reactions catalyzed by autophagy-related proteins (ATGs). (hindawi.com)

Poly(ADP-ribose) polymerase-1 (PARP-1) is an abundant and ubiquitous nuclear protein that uses NAD + to synthesize a multibranched polyanion composed of ADP-ribose moieties, giving rise to poly(ADP-ribose) (PAR), onto itself or a variety of target proteins. (nature.com)
One strategy that has been used to isolate the increasing array of non-antibody biopharmaceuticals is to tag target proteins and capture based upon the affinity-tag. (avacta.com)

Another group of enzymes, more specifically ubiquitin protein ligases called E3, bind to the misfolded protein. (wikipedia.org)
The tail anchored membrane protein Ubc6 as well as Ubc1 and the Cue1 dependent membrane bound Ubc7 are the ubiquitin conjugating enzymes involved in ERAD. (wikipedia.org)
This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)
Using his ribosome profiling approach, Weissman and his lab can measure protein production in both eukaryotic and prokaryotic organisms, and monitor when molecular chaperones, targeting factors and processing enzymes interact with nascent amino acid chains. (mit.edu)
These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades (e.g., the blood-clotting cascade, the complement system, apoptosis pathways, and the invertebrate prophenoloxidase-activating cascade). (stratech.co.uk)
Proteases determine the lifetime of other proteins playing an important physiological role like hormones, antibodies, or other enzymes-this is one of the fastest "switching on" and "switching off" regulatory mechanisms in the physiology of an organism. (stratech.co.uk)
Mainly, two genes linA and linB encode dehydrochlorinase and dehalogenase enzymes involved in degradation of HCH. (ispub.com)
Enzymes assembled from related degradation pathways into our host strain serve as the function part. (igem.org)
We introduce popular components involved in chemotaxis, quorum-sensing to regulatory parts, sense the environment signal, respond to move, accelerate growing and produce related degradation enzymes. (igem.org)
In the second step of the upper pathway BphB enzymes catalyzes the conversion of dihidrodiol to dihydroxy compound. (igem.org)
In drug discovery, it is of utmost importance to accurately calculate the free energies of binding ligands to various protein targets, such as enzymes and receptors. (lu.se)

This work demonstrates that mammalian metabolism is amenable to restoration of ancient core pathways, paving a path for genome-scale efforts to synthetically restore metabolic functions to the metazoan lineage. (elifesciences.org)
Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
STUB1 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Cell Cycle, Cellular Stress, Cytoplasmic Tyrosine Kinases, and Metabolic Disorder research. (rockland.com)
The major identified metabolic pathways are oxidation and degradation of the imidazole and piperazine rings, oxidative Odealkylation and aromatic hydroxylation. (nih.gov)
Multidrug tolerance results from drug-induced quiescence, which enables parasites to survive exposure to unrelated antimalarial drugs that inhibit a variety of metabolic pathways. (cdc.gov)
Biochemical genetic testing and newborn screening are essential laboratory services for the screening, detection, diagnosis, and monitoring of inborn errors of metabolism or inherited metabolic disorders. (cdc.gov)

Proteins that do not bind the receptor are directed to lysosomal degradation. (nature.com)

The first checkpoint is called ERAD-C and monitors the folding state of the cytosolic domains of membrane proteins. (wikipedia.org)
Not only membrane proteins surviving the first checkpoint are controlled for their luminal domains, also soluble proteins are inspected by this pathway as they are entirely luminal and thus bypass the first checkpoint. (wikipedia.org)
Membrane proteins must be threaded co-translocationally into the lipid bilayer to become membrane-integrated, often with complex topologies and typically form hetero- or homo- oligomers. (stanford.edu)
15. KulkarniRD, KelkarHS, DeanRA (2003) An eight-cysteine-containing CFEM domain unique to a group of fungal membrane proteins. (prelekara.sk)
Retention of cholesterol results in increased cholesterol content of membranes that reduces their fluidity and impairs the function of integral membrane proteins. (medscape.com)

In this report, the authors devised synthetic genomic strategies to introduce essential amino-acid biosynthetic pathways into mammalian cells. (elifesciences.org)
Altogether, this work was found to be of substantial interest as it provides pioneering evidence that mammalian systems may be permissive to the restoration of essential amino acid biosynthetic pathways and is thus anticipated to have a broad impact in the fields of synthetic biology, biotechnology and beyond. (elifesciences.org)
The biosynthetic pathways of RIN and IRN are largely unknown. (biomedcentral.com)

One pathway leading to apoptosis, the so-called extrinsic or death receptor pathway, involves tumor cell killing that is initiated by cytotoxic T lymphocytes and natural killer cells. (mayo.edu)
The other major pathway leading to apoptosis is the intrinsic pathway, called the mitochondrial pathway. (mayo.edu)

We designed codon-optimized biosynthesis pathways based on genes mined from Escherichia coli . (elifesciences.org)
Briefly, miRNAs are expected to account for 1-5% of the human genome and to interfere with at least 30% of the protein-coding genes ( 4 , 5 ). (frontiersin.org)
The lab also builds innovative tools for exploring organizational principles of biological systems including ribosome profiling, which globally monitors protein translation, and CRISPRi/a for controlling the expression of human genes and rewiring the epigenome. (mit.edu)
While technologies like RNA-seq have made it much easier to monitor which genes are being expressed in a cell at any given time, these approaches use messenger RNA as a proxy for proteins. (mit.edu)
Weissman also seeks to understand the ways different pathways in a cell interact with each other, and how genes function together in both normal and disease states. (mit.edu)
Bioinformatics strategies were used to find other homologous genes and proteins, which can also degrade HCH present in various bacteria for controlling of pollution and offer clue to prevent its entry in the food chain. (ispub.com)
In the present study bioinformatics analysis demonstrated cardiac hypertrophy signaling may be the most important pathway for upregulating genes in sesamin-treated groups. (spandidos-publications.com)

Endoplasmic-reticulum-associated protein degradation (ERAD) designates a cellular pathway which targets misfolded proteins of the endoplasmic reticulum for ubiquitination and subsequent degradation by a protein-degrading complex, called the proteasome. (wikipedia.org)
In this talk the important biological considerations for initiation of a degradation project, our platform approach for therapeutic degrader development, and the approaches we apply to functionally characterize these compounds in relevant cellular context. (fimecs.com)
Enable sensitive quantitation of PROTAC-mediated degradation of targets using Enzyme Fragment Complementation (EFC)-based cellular biosensor cell lines which combine EFC detection technology with CRISPR genome editing in our SPRINTer™ Protein Turnover Biosensor Assays. (fimecs.com)
But generally it is proteins, and not RNAs, that mediate a gene's cellular functions. (mit.edu)
Additionally - because the endoplasmic reticulum (ER) integrates lipid biosynthesis, protein folding, glycosylation, quality control and degradation and plays a key role in cellular homeostasis, infection, and immune defense - Weissman's research encompasses all aspects of ER function. (mit.edu)
Ratiometric imaging of GFP-ODD and a red- fluorescent reference protein reveals tissue-specific differences in the cellular hypoxic status at ambient normoxia. (unifr.ch)
After the degradation of damaged proteins and lipids, amino acids and fatty acids are released into the cytoplasm and recycled for new biosynthesis of cellular components or energy production [ 4 ]. (hindawi.com)

To use this resource, a user provides the sequence information for each protein building block and available partial or homologous structures. (asbmb.org)
Both homologous recombination, in which the ends of the broken DNA seek out intact templates with the same sequence, and nonhomologous end-joining pathways are found in Saccharomyces as they are in humans. (brandeis.edu)
This "in vivo biochemistry" approach has enabled us to demonstrate that there are in fact several independent, competing pathways of homologous recombination, each with its own genetic requirements. (brandeis.edu)
The carboxyl-terminal 64 aa of gamma(1)34.5 protein are homologous to the corresponding domain of MyD116, the murine growth arrest and DNA damage gene 34 (GADD34) protein and the two domains are functionally interchangeable in infected cells. (scienceopen.com)
In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. (nih.gov)

For development of Redistribution assays, BioImage (www.bioimage.com) uses Aequorea victoria GFP and other fluorescent proteins fused to translocating targets of interest. (genengnews.com)
G protein-coupled receptors (GPCRs) account for some 50 percent of all current drug targets, says Arne Heydorn, scientist at BioImage, whereas marketed drugs only target about 30 percent of the GPCRs in the genome. (genengnews.com)
Targeted protein degradation as a therapeutic modality has experienced an explosion in research the past several years resulting in advancements of many degraders to the clinic as well as opening possibilities in terms of targets and disease indications. (fimecs.com)
Growth arrest and DNA damage-inducible protein GADD34 targets protein phosphatase 1 alpha to the endoplasmic reticulum and promotes dephosphorylation of the alpha subunit of eukaryotic translation initiation factor 2. (scienceopen.com)

This led to the identification of 3 different pathways that constitute in fact 3 checkpoints. (wikipedia.org)

It is formed by thermal decomposition of chlorinated solvents, e.g., chloroform, carbon tetrachloride, tri- and tetra-chloroethylene and methylene chloride, as well as by the thermal degradation of chlorinated polymers. (inchem.org)

Furthermore, irradiation-induced pyroptosis is triggered by GSDME, which are cleaved by activated caspase-3 in the intrinsic mitochondrial pathway. (biomedcentral.com)

The APC protein, which comprises of 2843 amino acids, plays an important role in the β-catenin nuclear localization ( 8 ). (spandidos-publications.com)
Major genomic deletions in independent eukaryotic lineages have led to repeated ancestral loss of biosynthesis pathways for nine of the twenty canonical amino acids. (elifesciences.org)
Whole genome sequencing across the tree of life has revealed the surprising observation that nine essential amino acid (EAA) biosynthesis pathways are missing from the metazoan lineage ( Payne and Loomis, 2006 ). (elifesciences.org)
Using EBNA3C amino acids 365-545 in a yeast two hybrid screen, we found an interaction with the Growth Arrest and DNA-damage protein, Gadd34. (scienceopen.com)
A protease is an enzyme that conducts proteolysis, i.e., the protein catabolism by hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain which form the protein. (stratech.co.uk)
Proteases are involved in digesting long protein chains into short fragments, splitting the peptide bonds that link amino acid residues. (stratech.co.uk)
Some of them can detach the terminal amino acids from the protein chain (exopeptidases, such as aminopeptidases, carboxypeptidase A), while others attack internal peptide bonds of a protein (endopeptidases, such as trypsin, chymotrypsin, pepsin, papain and elastase). (stratech.co.uk)
Proteases can either break specific peptide bonds, depending on the amino acid sequence of a protein, or break down a complete peptide to amino acids. (stratech.co.uk)
Bacteria also secrete proteases to hydrolyze (digest) the peptide bonds in proteins and therefore break the proteins down into their constituent monomers (amino acids). (stratech.co.uk)
The encoded protein shares a high degree of homology to clostridial FMN- and FAD-dependent 2-enoate reductases, including the cinnamic acid reductase proposed to be involved in amino acid metabolism in proteolytic clostridia. (microbiologyresearch.org)

In HERA, rescue from degradation depends on FcRn, and engineered ligands are recycled in a manner that correlates with their half-lives in human FcRn transgenic mice. (nature.com)
Similarly, repeated exposure to the harsh chemical conditions of elution and column cleaning procedures results in degradation and leaching of the affinity ligands, reducing column capacity and stunting optimal process performance. (avacta.com)

This study revealed proteins involved in oxidative stress are able to inhibit p53 degradation (4). (novusbio.com)
Workplace exposures equivalent to no or low observable proteins and other molecular chaperones (valosin-containing pro- adverse effect concentrations in animals: Step by step tein or VCP) are up-regulated to handle the increase of misfolded approach and damaged proteins which are causing oxidative stress. (cdc.gov)

The net impact of nutritional regulation of protease activity among the thousands of species present in soil can be observed at the overall microbial community level as proteins are broken down in response to carbon, nitrogen, or sulfur limitation. (stratech.co.uk)
Gene technology, combined with a solid knowledge of catabolic pathways and microbial physiology, enables the experimental evolution of new or improved catabolic activities for such pollutants. (igem.org)
Source: Kensuke Furukawa and Hidehiko Fujihara, Microbial Degradation of Polychlorinated Biphenyls: Biochemical and Molecular Features , Journal of bioscience and bioengineering , Vol. 105, No. 5, 433-449. (igem.org)

PARP-1 is the best-characterized member of the diphtheria toxin-like ADP-ribosyl transferases (ARTDs) family of proteins. (nature.com)

Eventually EDEM will target the misfolded glycoproteins for degradation by facilitating binding of ERAD lectins OS9 and XTP3-B. Because the ubiquitin-proteasome system (UPS) is located in the cytosol, terminally misfolded proteins have to be transported from the endoplasmic reticulum back into cytoplasm. (wikipedia.org)
In addition to its role as a transcription factor, p53 functions in the cytoplasm to regulate the activity of apoptotic pathway proteins such as BAX, BAK , and BCL-2 (2). (novusbio.com)
Two of these family members, BAX and BAK, induce cell death by punching holes in the outer mitochondrial membrane, leading to the release of mitochondrial proteins to the cytoplasm, where they activate caspases. (mayo.edu)

Indeed, it was confirmed that soluble, membrane and transmembrane proteins were recognized by different mechanisms. (wikipedia.org)
Although the mechanisms are still unclear, p53 is thought to regulate these apoptotic proteins through direct interaction (2). (novusbio.com)
The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
What is involved in protein folding mechanisms, and what is the role improper protein folding can play in disease? (mit.edu)
Building on these earlier results, current studies in our lab focus on understanding the pathways that lead to caspase activation and the mechanisms that regulate those pathways. (mayo.edu)
Combined with the other studies in the Anticancer Drug Action Lab, our studies of apoptotic pathways are providing new insight into factors that govern cancer cell sensitivity to targeted anti-cancer agents and immunotherapy, while also elucidating the mechanisms of resistance to a variety of novel anti-cancer agents. (mayo.edu)
Dr. Marchese has had a long-standing interest in understanding the molecular mechanisms governing G protein-coupled receptor signaling. (mcw.edu)
Research in the Marchese lab is directed towards understanding the molecular mechanisms governing G protein-coupled receptor (GPCR) signaling. (mcw.edu)

A polyubiquitinated protein is produced and this is recognized by specific subunits in the 19S capping complexes of the 26S proteasome. (wikipedia.org)
CHIP acts as a molecular switch for the degradation of misfolded proteins via the proteasome and lysosome pathways (2). (rockland.com)

Most evidence suggest that the Hrd1 E3 ubiquitin-protein ligase can function as a retrotranslocon or dislocon to transport substrates into the cytosol. (wikipedia.org)
CHIP E3 ubiquitin ligases act sequentially in ER membrane and cytosol to monitor the folding status of CFTR and delF508.CHIP possesses intrinsic E3 ubiquitin ligase activity and promotes ubiquitylation. (rockland.com)
The fully functional autolysosome then degrades the autolysosomal cargo and releases the degradation products into the cytosol for recycling. (hindawi.com)

Our current studies are designed to better understand the process of BAK activation and the mechanism by which this critical pro-apoptotic protein permeabilizes the outer mitochondrial membrane. (mayo.edu)

When protein interactions occur in intrinsically disordered regions, it's often through short linear motifs, known as SLiMs, which are both tedious and challenging to study. (asbmb.org)

Ultimately, the autophagosome fuses with a lysosome, facilitated by Rab7 and LAMP proteins. (hindawi.com)

The focus of this study is the role of protein kinase Chk1 and the phosphatase Cdc25A in the DNA replication checkpoint. (vt.edu)
The model was updated with additional interactions, such as those including Myt1, a second inhibitor kinase, and lamin proteins, which become phosphorylated at the onset of nuclear envelope breakdown (NEB) at entry into mitosis. (vt.edu)
The gamma(1)34.5 protein of herpes simplex virus 1 complexes with protein phosphatase 1alpha to dephosphorylate the alpha subunit of the eukaryotic translation initiation factor 2 and preclude the shutoff of protein synthesis by double-stranded RNA-activated protein kinase. (scienceopen.com)
In human cells infected with herpes simplex virus 1 the double-stranded RNA-dependent protein kinase (PKR) is activated but phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2) and total shutoff of protein synthesis is observed only in cells infected with gamma(1)z34.5- mutants. (scienceopen.com)
Our research team previously showed that death receptors, which must be present on the cell surface in order for this pathway to be activated, are inhibited from going to the cell surface when the signaling molecule protein kinase Cβ is activated. (mayo.edu)

CHIP (STUB1) or STIP1 homology and U-box containing protein 1 (also known as E3 ubiquitin protein ligase) is an ubiquitin ligase/cochaperone that participates in protein quality control by targeting a broad range of chaperone protein substrates for degradation (1). (rockland.com)

Previous studies have highlighted that EAA biosynthesis tends to be more energetically costly, raising the possibility that these pathways were lost from organisms with access to abundant EAAs. (elifesciences.org)
13 C-tracing verified de novo biosynthesis of valine and further revealed build-up of pathway intermediate 2,3-dihydroxy-3-isovalerate. (elifesciences.org)

Together, our work explains how Retro-2 prevents retrograde trafficking of toxins by inhibiting TA-protein targeting, describes a general CRISPR strategy for predicting the MOA of small molecules, and paves the way for drugging the TRC pathway to treat broad classes of viruses known to be inhibited by Retro-2. (elifesciences.org)
Small non-protein-coding RNA molecules, composed of around 22 nucleotides, are commonly named as miRNAs ( 1 - 3 ). (frontiersin.org)
In particular, the authors monitored two specific molecules, RIG-1 and STAT1, involved in interferon signaling and found that the levels of these proteins correlate with viral loads. (asbmb.org)

The ubiquitination of terminally misfolded proteins is caused by a cascade of enzymatic reactions. (wikipedia.org)
Regulation of peroxisomal matrix protein import by ubiquitination. (ruhr-uni-bochum.de)

The Hippo pathway regulates two highly conserved, transcription co-activators YAP and TAZ. (selectbiosciences.com)
Chk1 regulates cell cycle arrest in the presence of unreplicated DNA in somatic cells by phosphorylating Cdc25A and leading to its degradation. (vt.edu)

Specifically, he and colleagues at UCSF developed CRISPRi, a method that uses the CRISPR-associated catalytically inactive dCas9 protein as a way to precisely regulate gene expression. (mit.edu)

This study extends our understanding of the pathway of host heme utilization by fungal pathogens, and provides new insights into the question of how nutrients such as heme cross the fungal cell wall. (prelekara.sk)

Transgenes can be designed in silico , assembled from validated DNA parts and inserted at precise and predetermined locations within the chloroplast genome to give stable synthesis of a desired recombinant protein. (microbiologyresearch.org)

Numerous recent examples of different therapeutic proteins produced successfully in the C. reinhardtii chloroplast highlight the potential of this green alga as a simple, low-cost and benign host. (microbiologyresearch.org)
In this paper we review the current status of algal chloroplast engineering with respect to therapeutic proteins. (microbiologyresearch.org)
Protein tagging is often undesirable in itself- particularly for therapeutic proteins where the affinity-tag may induce immunogenicity. (avacta.com)

Here we show that efficient heme uptake relies on a family of extracellularly-anchored proteins that serve as heme receptors, two of which, at least, are required for efficient heme utilization. (prelekara.sk)
Our data suggest the existence of a relay system that transfers heme from one protein to the next across the cell envelope, explaining the requirement for multiple heme receptors for efficient heme-iron utilization. (prelekara.sk)

CRISPRi genetic interaction analysis revealed Retro-2 activity resembles disruption of the transmembrane domain recognition complex (TRC) pathway, which mediates post-translational ER-targeting and insertion of tail-anchored (TA) proteins, including SNAREs required for retrograde transport. (elifesciences.org)

The GCX, which is major constituent of the ESL, forms a luminal mesh that provides endothelial cells with a framework to bind plasma proteins and soluble GAGs. (biomedcentral.com)

The applications of p53 antibodies range from assessing protein levels and post-translational modifications to examining interactions with binding partners or occupancy at a gene locus. (novusbio.com)
Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. (nih.gov)

To overcome these shortcomings, Evelyn Ramberger, Lorena Suarez-Artiles, Daniel Perez-Hernandez and colleagues at Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Germany, have developed an optimized method for using protein interaction screen on a peptide matrix, or PRISMA, in combination with quantitative mass spectrometry. (asbmb.org)
Körschgen H, Baeken M, Schmitt D, Nagel H, Behl C . Co-chaperone BAG3 enters autophagic pathway via its interaction with microtubule associated protein 1 light chain 3 beta. (unimedizin-mainz.de)
10.1016/j.toxlet.2010.03.267 expressed and the interaction was analysed with protein network software. (cdc.gov)

Amplite™ Universal Fluorimetric Protease Activity Assay Kit is an ideal choice to perform routine protease isolation assays or for identifying the presence of contaminating proteases in protein samples. (stratech.co.uk)

This eventually leads to an inhibition of replication, transcription and translation of the viral genome, followed by its degradation and recruitment of immune cells. (asbmb.org)

Extracts containing these terpene indole alkaloids (TIAs) can inhibit the formation and destabilize preformed fibrils of amyloid β protein (a pathological marker of Alzheimer's disease), and have been shown to improve the cognitive function of mice with Alzheimer-like symptoms. (biomedcentral.com)

The selective degradation of sirtuins via macroautophagy in the MPP + model of Parkinson's disease is promoted by conserved oxidation sites. (unimedizin-mainz.de)

These observations, which are currently being explored in greater detail, lay the foundation for efforts to increase the efficacy of immunotherapy by modulating the death receptor pathway in various tumors. (mayo.edu)
In addition, our recent studies have implicated the death receptor pathway in the TP53-independent anti-leukemic effects of inhibitors of the kinases Chk1, ATR and Wee1 as single agents. (mayo.edu)
Phosphorylation of the receptor protein Pex5p modulates import of proteins into peroxisomes. (ruhr-uni-bochum.de)
The deubiquitination of the PTS1-import receptor Pex5p is required for peroxisomal matrix protein import. (ruhr-uni-bochum.de)

We conjugated the oxygen-sensing transcription activation domain (TAD) of hypoxia-inducing factor (HIF-1α) with the Cas9/dCas9 protein. (bvsalud.org)

When unreplicated or damaged DNA is present, cell cycle checkpoint pathways cause cell cycle arrest by inhibiting cyclin-dependent kinases (Cdks). (vt.edu)

Lipid and protein content profiling of isolated native autophagic vesicles. (unimedizin-mainz.de)

Peritoneal stomata constitute the principal pathways for the drainage of intraperitoneal contents from the PERITONEAL CAVITY to the LYMPHATIC SYSTEM. (bvsalud.org)

It is unclear whether present-day metazoans can reaccept these pathways to resurrect biosynthetic capabilities that were lost long ago or whether evolution has rendered EAA pathways incompatible with metazoan metabolism. (elifesciences.org)
1993). The acetylated and glucuronidated forms of AAs, which are excreted directly in urine, are the products of the body's detoxification metabolism pathway. (cdc.gov)

In vitro , the plasma protein binding is about 99% mainly to the albumin fraction. (nih.gov)

When the cytosolic domains are found to be correctly folded, the membrane protein will pass to a second checkpoint where the luminal domains are monitored. (wikipedia.org)

These results indicate that in infected cells, gamma(1)34.5 interacts with and redirects phosphatase to dephosphorylate eIF-2alpha to enable continued protein synthesis despite the presence of activated PKR. (scienceopen.com)
The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. (nih.gov)

Western blotting or immunofluorescence with p53 antibodies is also widely used to monitor induction of DNA damage pathways (5). (novusbio.com)
Protein A affinity purification is the accepted industry standard for purifying monoclonal antibodies, yet multiple problems (link is external) present with industry use of this affinity ligand. (avacta.com)

To limit these processes with the initiation of smart monitoring systems would require an increased understanding of the factors that influence protein A affinity purification performance loss, though this knowledge is not currently available. (avacta.com)

We are interested in understanding at the molecular level how recombination occurs and what roles are played by the many proteins involved in DNA recombination, repair and replication. (brandeis.edu)

Total 38 nucleotides and 36 proteins sequences of linA and linB were used for constructing the phylogeny. (ispub.com)

EBNA3C is an EBV-encoded nuclear protein, essential for proliferation of EBV infected B-lymphocytes. (scienceopen.com)
When both proteins are overexpressed, Gadd34 can interact with EBNA3C in both nuclear and cytoplasmic compartments. (scienceopen.com)
A cell-penetrant peptide blocking C9ORF72-repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins. (le.ac.uk)

We are interested in determining what are the specific biochemical roles played by the many proteins implicated in DNA recombination, repair and replication. (brandeis.edu)

This converging pathway makes the differentiation of cholestatic diseases on clinical grounds very difficult. (medscape.com)

Our goal is to elucidate the functional networks that coordinate protein synthesis and quality control in the early secretory pathway. (stanford.edu)
We have identified the proteins necessary to carry out the initial steps in strand invasion and the beginning of new DNA synthesis, which is significantly different from the normal process of replication. (brandeis.edu)
The proposed mechanism for maintenance of protein synthesis in the face of double-stranded RNA accumulation is different from that described for viruses examined to date. (scienceopen.com)

Interrogating the degradation pathways of unstable mRNAs with XRN1-resistant sequences. (nih.gov)

If defects are detected in the cytosolic domains, this checkpoint will remove the misfolded protein. (wikipedia.org)
This second checkpoint is called the ERAD-L pathway. (wikipedia.org)
The use of checkpoint inhibitors in combination therapy further complicates the safety monitoring of SKM injury due to the potential for drug-drug interactions. (degruyter.com)
One goal of this work is to understand the developmental role and regulation of checkpoint signaling pathways due to its monitoring of DNA integrity within the cell. (vt.edu)

Anatomy22

Organisms18

Diseases1

Chemicals and Drugs118

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes65

Disciplines and Occupations1

Information Science4

Health Care3

Intracellular11

Proteasomal3

Autophagy8

Target proteins2

Enzymes11

Metabolic6

Lysosomal degradation1

Membrane proteins5

Biosynthetic pathways3

Apoptosis2

Genes7

Cellular7

Homologous5

Targets4

Different pathways1

Thermal degradation1

Mitochondrial pathway1

Amino10

Ligands2

Oxidative stress2

Microbial3

Family of proteins1

Cytoplasm3

Mechanisms8

Proteasome2

Cytosol3

Apoptotic1

Intrinsically disordered1

Lysosome1

Kinase5

Ubiquitin-protei1

Biosynthesis2

Molecules3

Ubiquitination2

Regulates2

Regulate1

Pathogens1

Recombinant Protein1

Therapeutic3

Receptors2

Tail anchored1

Soluble1

Gene2

Interaction3

Assay1

Genome1

Inhibit1

Selective1

Receptor4

DCas9 protein1

Cyclin-depende1

Lipid1

Constitute1

Metabolism2

Albumin1

Correctly folded1

Interacts2

Antibodies2

Initiation1

Occurs1

Nucleotides1

Nuclear3

Biochemical1

Differentiation1

Synthesis3

Sequences1

Checkpoint4