Degradation of aromatic enzymes catechol 2 3 dioxygenase. Medical search. Frequent questions

Polycyclic aromatic hydrocarbons (PAHs) degrading bacteria can be isolated from polluted coastal environments. (omicsonline.org)
Polycyclic aromatic hydrocarbons (PAHs) are one class of organic pollutants that are considered the most hazardous due to their toxic, mutagenic, and carcinogenic properties [ 1 ]. (omicsonline.org)
The analysis of both the metagenome of the consortium and the reconstructed metagenome-assembled genomes show that the most abundant bacterial genus in the consortium, Acidocella , possess many of the genes required for the degradation of diesel fuel aromatic hydrocarbons, which are often the most toxic component. (springeropen.com)
and for degradation of AROMATIC HYDROCARBONS . (nih.gov)
able to significantly ( P = .05) utilize the all the aromatic hydrocarbons. (journalarrb.com)

PAHs could be degraded by bacteria under aerobic conditions through the initial oxidation of the aromatic ring, which is catalyzed by the dioxygenase enzyme. (omicsonline.org)
Plant-mediated effects on polycyclic aromatic hydrocarbon (PAH) degradation by bacteria in the rhizosphere of the salt marsh grasses, Spartina alterniflora and Phragmites australis. (nih.gov)
A number of bacteria that can degrade a variety of aromatic compounds have been identified and the pathways involved in the degradation have been extensively characterized [ 3 , 4 ]. (biomedcentral.com)
Peripheral enzymes, particularly oxygenases and dehydrogenases, were found to transform structurally diverse substrates into one of these central intermediates by bringing about the hydroxylation of the aromatic nucleus (Fig. 2A ), and hence it is thought that bacteria have developed these enzymes to extend their substrate range [ 5 ]. (biomedcentral.com)
Enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelactone. (inrae.fr)
The root-mean-square deviation from ideal bond distances for the model is 0.014 A and the error in atomic co-ordinates is estimated to be 0.15 A. DLH is a monomeric enzyme containing 236 amino acid residues and is a member of the beta-ketoadipate pathway found in bacteria and fungi. (inrae.fr)
To explore the phylogenetic framework of bacteria isolated from contaminated marine environments of Niger Delta and the expression of the metabolic genes coding for aromatic hydrocarbon degradation and surfactant production. (journalarrb.com)
A laboratory scale study was carried on six composite samples of the sediment and water samples from the three studied areas using enrichment, screening, selection, characterization, and PCR assays to explore the phylogenetic framework and metabolic genes expression of the marine bacteria for aromatic hydrocarbon degradation and surfactant production. (journalarrb.com)
The existence of catabolic and surfactant genes namely catechol dioxygenase ( C23O ), rhamnolipid enzyme ( rhlB ) and surfactin /lichenysin enzyme ( SrfA3 /LicA3 ) genes were detected in only four (4) out of the nine (9) marine aromatic degrading bacteria with 881 base pairs sizes. (journalarrb.com)

2003. Characterization of a novel Acinetobacter species containing a toluene dioxygenase. (nih.gov)
Substrate specificity of catechol oxidase from Lycopus europaeus and characterization of the bioproducts of enzymic caffeic acid oxidation. (lookformedical.com)
1. Fischer, D., Ebenau-Jehle, C. and Grisebach, H. Phytoalexin synthesis in soybean: purification and characterization of NADPH:2'-hydroxydaidzein oxidoreductase from elicitor-challenged soybean cell cultures. (qmul.ac.uk)

They isolated a Pseudomonas azelaica strain HPB1 which was able to degrade OPP through the production of 2,3-dihydroxybiphenyl. (nature.com)
His primary areas of investigation include Organic chemistry, Toluene dioxygenase, Pseudomonas putida, Biochemistry and NAD+ kinase. (research.com)
The concepts of his Pseudomonas putida study are interwoven with issues in Escherichia coli, Electron donor, Dioxygenase, Dehydrogenase and Naphthalene. (research.com)
Stereochemistry, Organic chemistry, Biochemistry, Enzyme and Pseudomonas putida are his primary areas of study. (research.com)
His Pseudomonas putida research is multidisciplinary, incorporating elements of Nuclear chemistry, Escherichia coli, Toluene, Chromatography and Toluene dioxygenase. (research.com)
His Escherichia coli research integrates issues from Regioselectivity, Microorganism, Pseudomonas putida, Metabolite and Toluene dioxygenase. (research.com)
Alkaline cyanide degradation by Pseudomonas pseudoalcaligenes CECT5344 in a batch reactor. (paperbase.net)
Metabolism of 2,4,6-trinitrotoluene by Pseudomonas sp. (paperbase.net)

Dienelactone hydrolase (DLH), an enzyme from the beta-ketoadipate pathway, catalyzes the hydrolysis of dienelactone to maleylacetate. (inrae.fr)

2006. Microbial dioxygenase gene population shifts during polycyclic aromatic hydrocarbon biodegradation. (nih.gov)

2-Hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85, xylG [gene], praB [gene] ) is an enzyme with systematic name (2E,4Z)-2-hydroxy-6-oxohexa-2,4-dienoate:NAD+ oxidoreductase. (wikipedia.org)
Moreover, a dioxygenase gene, nidA3B3, was detected in Mycobacterium vanbaalenii PYR-1 as an alternate degradation pathway which could catalyze both the initial dihydroxylation of pyrene [ 11 ] to be pyrene cis-4,5-dihydrodiol, and an alternate detoxification pyrene pathway to be pyrene cis-1,2-dihydrodiol [ 12 ]. (omicsonline.org)
6 identified a gene cluster, hbpCAD , encoding the upper metabolic pathway of OPP which involves the transformation of OPP to 2-hydroxypenta-2,4-dienoateand benzoic acid (BA). (nature.com)
Each enzyme entry contains its common name and synonym, reaction in which enzyme is involved, family and subfamily, structure and gene link and literature citation. (biomedcentral.com)
For all significantly differentially expressed microRNAs inside of the 2 groups, we now have produced gene sets from their expressed target genes. (pdpksignaling.com)
Catechol 2,3-dioxygenase (C23O) gene was found andamplified with the designed primers from the total DNA of C-14-1. (researchgate.net)

This enzyme catalyses the following chemical reaction 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O ⇌ {\displaystyle \rightleftharpoons } (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+ This substrate for this enzyme is formed by meta ring cleavage of catechol (EC 1.13.11.2, catechol 2,3-dioxygenase), and is an intermediate in the bacterial degradation of several aromatic compounds. (wikipedia.org)
low-molecular-weight (LMW) compounds consist of two or three rings, and high-molecular-weight (HMW) compounds, more than three rings [ 3 ]. (omicsonline.org)
Microbial degradation of organic compounds. (research.com)
They play a key role in the metabolism of organic compounds by increasing their reactivity or water solubility or bringing about cleavage of the aromatic ring. (biomedcentral.com)
Many of these compounds have been reported to be toxic to the living organisms [ 3 ]. (biomedcentral.com)
In the convergent mode, structurally diverse aromatic compounds are converted to one of a few aromatic ring cleavage substrates such as catechol, gent sate, protocatechuate and their derivatives [ 5 ]. (biomedcentral.com)
Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)
Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
Comment: (2Z)-2-hydroxypenta-2,4-dienoate (HPD) is a common intermediate in the aerobic degradation of many aromatic compounds. (lbl.gov)

David T. Gibson has included themes like Plasmid, DNA, Cleavage and Hydroquinone in his Enzyme study. (research.com)
In this divergent mode, a metal-dependent dioxygenase channels these dihydroxylated intermediates into one of the two possible pathways: the meta -cleavage pathway or the ortho -cleavage pathway [ 7 - 9 ] (Fig. 1 ). (biomedcentral.com)
Dienelactone hydrolases play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. (inrae.fr)
Comment: In MetaCyc pathway catechol degradation to HPD I (meta-cleavage, link ), dioxygenase xylE converts catechol to (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate (also known as 2-hydroxymuconate 6-semialdehyde). (lbl.gov)
In MetaCyc pathway catechol degradation III (ortho-cleavage, link ), the 1,2-dioxygenase catA forms cis,cis-muconate, a cycloisomerase forms (+)-muconolactone, an isomerase converts this to (4,5-dihydro-5-oxofuran-2-yl)-acetate (also known as 3-oxoadipate enol lactone), and a hydrolase cleaves this to 3-oxoadipate. (lbl.gov)
This series of steps is part of protocatechuate para-cleavage, link , or catechol degradation II, link . (lbl.gov)

Oxygenases belong to the oxidoreductive group of enzymes (E.C. Class 1), which oxidize the substrates by transferring oxygen from molecular oxygen (O 2 ) and utilize FAD/NADH/NADPH as the co-substrate. (biomedcentral.com)
As the thiolate anion is not energetically favoured in the hydrophobic interior of the enzyme, it swings into the active site where it can be stabilized by the His202 imidazolium and the dipole of helix C. The Cys123 thiolate which now lies adjacent to the acyl carbon of the substrate, is thus generated only in the presence of substrate. (inrae.fr)
Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. (nih.gov)
The substrate specificity of catechol oxidase from Lycopus europaeus towards phenols is examined. (lookformedical.com)

Proteins/genes with a putative catabolic role and bacterium adaptation mechanisms during OPP degradation were identified via genomic and proteomic analysis. (nature.com)
The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon ( opp ) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon ( bph ). (nature.com)
An analysis of the genome and its transcriptome revealed enzymes with biotechnological potential, such as two extracellular tannases (Atan1p and Atan2p) of the tannic-acid catabolic route, and a new pathway for the assimilation of n-butanol via butyric aldehyde and butyric acid. (biomedcentral.com)
For example, pathway IV yields indole-3-lactate, which could potentially be oxidized to indole-3-acetate, which has a known catabolic pathway, but no prokaryotes are known to consume tryptophan this way. (lbl.gov)

Crystal structure of naphthalene dioxygenase: side-on binding of dioxygen to iron. (research.com)
An integrated approach of bioassay and molecular docking to study the dihydroxylation mechanism of pyrene by naphthalene dioxygenase in Rhodococcus sp. (brenda-enzymes.org)

The pcaH was significant in the β-ketoadipate pathway [9] where the pathway as it conserves biochemically, and is a major class of non-heme-iron containing dioxygenase [ 10 ]. (omicsonline.org)
However, the microbial metabolic pathway of the fungicide and the genetic systems driving its degradation by strain P3 remain unknown. (nature.com)
Pathway VIII yields tryptophol (also known as indole-3-ethanol), which could potentially be oxidized to indole-3-acetate and consumed. (lbl.gov)
Comment: In pathway I, dioxygenase kynA opens the non-aromatic ring, to N-formyl-L-kynureine, a hydrolase yields L-kynurenine (and formate), and a hydrolase yields anthranilate and L-alanine. (lbl.gov)
Comment: In MetaCyc pathway anthranilate degradation I ( link ), a dioxygenase cleaves off carbon dioxide and ammonia, leaving catechol. (lbl.gov)
In MetaCyc pathway anthranilate degradation IV ( link ), anthranilate hydroxylase/monooxygenase (hpaH) yields 3-hydroxyanthranilate. (lbl.gov)
Comment: 3-hydroxyanthranilate degradation is part of L-tryptophan degradation pathway XII ( link ). (lbl.gov)
Comment: MetaCyc pathway 3-oxoadipate degradation ( link ) involves activation by CoA (using succinyl-CoA) and a thiolase (succinyltransferase) reaction that splits it to acetyl-CoA and succinyl-CoA. (lbl.gov)
This is part of a MetaCyc pathway for catechol degradation, link . (lbl.gov)

Thus, the study revealed that these bacterial strains especially Serratia marcescens XYL7 might possess metabolic genes for in situ aromatic hydrocarbon degradation and surfactant production. (journalarrb.com)

Based on the complexity of the degradation pathways, the phenomenon of biodegradation is categorized into two types: convergent and divergent modes of degradation (Fig. 1 ). (biomedcentral.com)
Further, the centralized degradation pathways mean synthesis of fewer degradative enzymes requiring less metabolic energy. (biomedcentral.com)
Overview: Tryptophan degradation in GapMind is based on MetaCyc degradation pathways I via anthranilate ( link ), II via pyruvate ( link ), or IX via 3-hydroxyanthranilate ( link ). (lbl.gov)
Pathways X and XIII yield indole-3-propionate, which may spontaneously oxidize to kynurate, but kynurate catabolism is not reported. (lbl.gov)
Additional pathways are not included: the fate of 2-amino-5-oxocyclohex-1-enecarboxyl-CoA is not known ( link ), and anthraniloyl-CoA reductase (the only anaerobic route known, link ) has not been linked to sequence. (lbl.gov)

Taken with the present results, this suggests that the potential use of inhibitors of prolyl 4-hydroxylase to control excessive collagen deposition in pathological fibrosis may be limited by the need to maintain continuous inhibition of collagen hydroxylation so as to facilitate intracellular degradation of the accumulated protein. (embl.de)

Dioxygenase NbaC cleaves the aromatic ring, yielding 2-amino-3-carboxymuconate 6-semialdehyde, a decarboxylase forms (2Z,4E)-2-aminomuconate semialdehyde, a dehydrogenase forms (2Z,4E)-2-aminomuconate, a deaminase forms (3E)-2-oxo-3-hexenedioate (also known as 2-oxalocrotonate), and a decarboxylase forms (2Z)-2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)
Comment: Dehydrogenase praB forms 2-hydroxymuconate, tautomerase praC forms (3E)-2-oxohex-3-enedioate (2-oxalocrotonate), and decarboxylase praD yields 2-hydroxypenta-2,4-dienoate (HPD). (lbl.gov)

Because levodopa bypasses the enzyme tyrosine hydroxylase , the rate-limiting step in dopamine synthesis, it is much more readily converted to dopamine than tyrosine, which is normally the natural precursor for dopamine production. (cloudfront.net)

Substrates containing a -COOH group are inhibitors for catechol oxidase. (lookformedical.com)
In vitro production of enzymatically active O. volvulus prolyl 4-hydroxylase should facilitate identification of specific inhibitors of the parasite enzyme. (embl.de)

His Toluene dioxygenase research includes themes of Indole test, Toluene, Spectrophotometry and Tryptophanase. (research.com)

Oxygenases can further be grouped into two categories i.e. monooxygenases and dioxygenases on the basis of number of oxygen atoms used for oxidation. (biomedcentral.com)
The enzyme catalyzes the oxidation of o-diphenols to o-quinones without hydroxylating monophenols, the additional activity of tyrosinase. (lookformedical.com)

To date little is known about the microbial degradation of OPP. (nature.com)
The microbial degradation of native lignin by basidiomyce- citrant and stable in the environment. (lu.se)

The United States Environmental Protection Agency indicated that implementing in situ degradation will result in cost savings of 50 to 80% over traditional methods such as excavation and landfill incineration (USEPA 2001 ). (springeropen.com)

Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. (wikipedia.org)
These results contribute to a better more biomass-derived carbohydrates are also used for produc- understanding of the aromatic metabolism of P. putida in tion of chemicals, a further valorization of lignin is an essential terms of growth and uptake rates, which will be helpful for component. (lu.se)

nidA and nidB genes are encoding genes for large and small subunits of napthalene-inducible dioxygenase [ 7 , 8 ]. (omicsonline.org)
B, solute carrier relatives six member 2, solute carrier relatives 18 member one, and transcription factors and homeobox genes involved in neural crest derived cell de velopment, paired like homeobox 2a and 2b, GATA binding protein two and 3, heart and neural crest derivatives expressed two. (pdpksignaling.com)

Comment: There are two forms of anthranilate dioxygenase, 3-subunit antABC or 4-subunit andAabcd. (lbl.gov)

An enzyme of the oxidoreductase class that catalyzes the reaction between catechol and oxygen to yield benzoquinone and water. (lookformedical.com)

The enzyme responsible for collagen maturation is prolyl 4-hydroxylase, making this enzyme a central activity in cuticle biosynthesis and a potentially important chemotherapeutic target. (embl.de)

In the pyrene and fluoranthene degradation experiment using a minimum medium, B. fungorum isolate no. 1 and M. gilvum isolate no. 13 were able to degrade 98.6% + 1.9 of pyrene after 20 days, and 99.9% ± 0.1 after a 16-day incubation period, respectively. (omicsonline.org)

His Enzyme research is included under the broader classification of Organic chemistry. (research.com)

16S lignin-derived mixtures of aromatic molecules. (lu.se)

In the reverse reaction, the 2'-hydroxyisoflavone (2'-hydroxydaidzein) is reduced to an isoflavanone. (qmul.ac.uk)

The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
l -3,4-dihydroxyphenylalanine , is an amino acid that is made and used as part of the normal biology of some plants [3] and animals, including humans. (cloudfront.net)

The enzyme exhibited activity that was slightly more tolerant to alkaline pH. (openmicrobiologyjournal.com)

Therefore, engineering of Catechol 2,3-dioxygenase may be employed for application on bioremediation of polluted sites. (openmicrobiologyjournal.com)

The entries are also linked to several external database including BRENDA, KEGG, ENZYME and UM-BBD providing wide background information. (biomedcentral.com)

this has seen reduction in SO 2 emissions across Europe by more than 60% between 1990 and 2004 (Vestreng et al. (springer.com)
reproductive enthusiastic feed 's the Common reduction of 1,3-diaminobenzenes from entirely gradual risks. (xn--drpverein-rahe-vpb.de)

Catechol degradation to HPD II also involves xylE and HPD, link . (lbl.gov)

Anatomy7

Organisms26

Diseases2

Chemicals and Drugs142

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes51

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care4

Hydrocarbons5

Bacteria9

Characterization3

Pseudomonas8

Beta-ketoadipate pathway1

Polycyclic1

Gene6

Compounds9

Cleavage6

Substrate4

Catabolic4

Naphthalene dioxygenase2

Pathway9

Hydrocarbon1

Pathways5

Hydroxylation1

Dehydrogenase2

Synthesis1

Inhibitors2

Toluene1

Oxidation2

Microbial degradation2

Situ1

Metabolism2

Genes2

Anthranilate1

Oxidoreductase1

Biosynthesis1

Pyrene1

Organic1

Molecules1

Reaction1

Amino acid2

Alkaline1

Bioremediation1

BRENDA1

Reduction2

Involves1