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  • fibroblast growth
  • 2. The method according to claim 1 wherein the population of DECs is produced by a method comprising: i) culturing a population of pluripotent cells (PSCs) in a definitive endoderm (DE) induction medium comprising a TGFβ ligand, fibroblast growth factor (FGF), bone morphogenetic protein (BMP) and a PI3K inhibitor, and ii) allowing the PSCs to differentiate into DECs. (patsnap.com)
  • 7. The method according to claim 2 wherein the DE induction medium consists of a chemically defined nutrient medium supplemented with a TGFβ ligand, fibroblast growth factor (FGF), bone morphogenetic protein (BMP) and a PI3K inhibitor. (patsnap.com)
  • marker
  • When used together with a published Nkx2.1-GFP knock-in ESC line, the protocol allows tracking and purification of lung and thyroid progenitors by sorting on the GFP reporter based on the induction of the earliest known marker of lung and thyroid cell fate, Nkx2.1. (bio-protocol.org)
  • support
  • These endoderm-derived organs support indispensible functions in adults, such as gas exchange in respiration, mechanical and chemical digestion, and blood glucose homeostasis and detoxification. (biomedcentral.com)