RegenerationAmputationExtracellular matrixLimbProliferativeEmbryonicZebrafishGenesApoptosisMechanismGene expressionAmphibianMiceDifferentiationWoundDownregulationPathwaysStumpChromatinTissueInsulinInteractionMesenchymalStemAdultInvestigating the regePyroptosisSignaling pathwayRemainProcessIssueDevelopmentTypes
Regeneration14
- In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
- The pathways discussed below are found to be closely related to dedifferentiation and regeneration in some species. (wikipedia.org)
- Because not one pathway has been elucidated as necessary for all dedifferentiation and regeneration, it is possible that the mechanism functions differently in different species. (wikipedia.org)
- The BMP pathway has been shown to be necessary for dedifferentiation and regeneration in tadpoles. (wikipedia.org)
- Lowered Notch1 expression resulted in no tadpole tail regeneration, and induced Notch1 expression was able to partially rescue tail regeneration in the form of notochord and spinal cord (but very little musculature. (wikipedia.org)
- Interestingly, in Xenopus limb buds rendered regeneration deficient by heat shock induced expression of transgenic noggin, chaperone gene expression is not really maintained since it is in wild style buds. (atpasesignaling.com)
- Nevertheless, 3 on the four transcription component genes used to reprogram mammalian grownup somatic cells to pluripo tency are upregulated all through blastema forma tion in regenerating newt limbs, as well as all through lens regeneration. (atpasesignaling.com)
- Which biological mechanisms guide the mature cells through the regeneration process? (unifr.ch)
- We are currently investigating which adhesion mechanisms organize the different cells types during fin regeneration. (unifr.ch)
- Epimorphic regeneration is the process by which complete regeneration of a complex structure such as a limb occurs through production of a proliferating blastema. (biomedcentral.com)
- Furthermore, the underlying mesenchyme remains rounded and does not expand to form a cone shaped blastema, a normal feature of successful regeneration. (biomedcentral.com)
- Epimorphic regeneration can be thought of as occurring in two phases: wound healing and cell proliferation. (biomedcentral.com)
- We have developed a heat shock inducible transgenic line ( N1 ) of Xenopus in which the BMP antagonist Noggin can be induced at a specific time during either development or regeneration, repressing BMP signalling [ 10 , 12 ]. (biomedcentral.com)
- We have used this line to show that BMP function is not only required for appendage regeneration but that it is specifically needed to generate a proliferating blastema while being dispensable for wound healing [ 12 ]. (biomedcentral.com)
Amputation4
- Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
- Just as plant cells respond to injury by undergoing callus formation via dedifferentiation, some animal models dedifferentiate their cells to form blastema, which are analogous to plant calluses, after limb amputation. (wikipedia.org)
- First, the fin margin is covered by a wound epithelium, and next a proliferative blastema arises by dedifferentiation of stump tissues near the amputation. (unifr.ch)
- N1 transgenic hindlimbs, which do not regenerate, do not form an apical epithelial cap or cone shaped blastema following amputation. (biomedcentral.com)
Extracellular matrix2
- For dedifferentiation, genes that are in the extracellular matrix play an important role. (wikipedia.org)
- MMP degrades proteins in the extracellular matrix [1] of a cell, resulting in the destabilization of the differentiated cell identity. (wikipedia.org)
Limb2
- Beyond this, little is identified concerning the molecular mechanism of dedifferentiation from the regener ating urodele limb. (atpasesignaling.com)
- Hence it can be possible that LIN28 may perform a part from the selleckchem transcriptional regulation of nuclear reprogramming dur ing limb cell dedifferentiation. (atpasesignaling.com)
Proliferative1
- The loss of specialization observed in dedifferentiation can be noted through changes in gene expression, physiology, function witin the organism, proliferative activity, or morphology. (wikipedia.org)
Embryonic1
- Our experiments revealed that the myocardium adjacent to the injury undergoes dedifferentiation, during which embryonic cardiac programs become reactivated to give rise to new tissue. (unifr.ch)
Zebrafish2
- Several years ago, our laboratory established a cryoinjury-induced myocardial infarction model in zebrafish, whereby a freezing-thawing procedure destroys approx. (unifr.ch)
- Using Affymetrix Gene Chip analysis, we have identified genes linked to regenerative success downstream of BMP signalling, including the BMP inhibitor Gremlin and the stress protein Hsp60 ( no blastema in zebrafish). (biomedcentral.com)
Genes3
- reported the upregulation of two genes connected with combating cell strain in regenerating axolotl limbs at four dpa. (atpasesignaling.com)
- Dedifferentiated cells express a number Resminostat price of genes related with all the dedifferentiated state, which include msx1, Nrad, rfrng and notch. (atpasesignaling.com)
- First of all, remedy of cultured insulin creating INS 1E cells with all the reversible ER stress inducer cyclopiazonic acid upregulated genes related to ER pressure when concurrently downregulating genes associated with differentiated cell functions. (atpasesignaling.com)
Apoptosis1
- Sec ondly, NO signaling inhibits apoptosis and induces ded ifferentiation of chondrocytes in vitro by means of p38 kinase and calveolin 1. (atpasesignaling.com)
Mechanism1
- The mechanism by which dedifferentiation occurs has not been completely illuminated. (wikipedia.org)
Gene expression1
- This is in contrast to differentiation, where differences in gene expression, morphology, or physiology arise in a cell, making its function increasingly specialized. (wikipedia.org)
Amphibian1
- The molecular characteri zation of blastema cell surface antigens and study of the regulation of dedifferentiation by transcription variables, microRNAs, polycomb proteins and chromatin modify ing enzymes is going to be essential for comprehending the mecha nism of dedifferentiation in regenerating amphibian limbs. (atpasesignaling.com)
Mice5
- For example, in mice myotubes, dedifferentiation is marked by a decreased expression of Myogenin, a protein present in differentiated myotubes. (wikipedia.org)
- The UPR is induced in mice trans genic for any mutation that leads to accumulation of mis folded collagen 101 chains from the hypertrophic chondrocytes of creating endochondral bones. (atpasesignaling.com)
- We demonstrate that deletion of the macrophage vitamin D receptor (VDR) in mice (KODMAC) is sufficient to induce insulin resistance by promoting M2 macrophage accumulation in the liver as well as increasing cytokine secretion and hepatic glucose production. (regenerativemedicine.net)
- Bone marrow transplant of VDR-expressing cells into KODMAC mice improved insulin sensitivity, suppressed atherosclerosis, and decreased foam cell formation. (regenerativemedicine.net)
- The immunomodulatory effects of vitamin D in macrophages are thus critical in diet-induced insulin resistance and atherosclerosis in mice. (regenerativemedicine.net)
Differentiation2
- Here, the concept of a marble moving downhill through various paths is used to represent cell decision-making and cell potency, thus visualizing how cells can take different paths of differentiation to reach a final state. (wikipedia.org)
- HIF-1α drives the transcription of NOG to inhibit osteogenic differentiation of periodontal ligament stem cells in response to hypoxia. (stembook.org)
Wound2
- While it can be induced in a laboratory setting through processes like direct reprogramming and the production of induced pluripotent stem cells, endogenous dedifferentiation processes also exist as a component of wound healing mechanisms. (wikipedia.org)
- Once the wound is healed, the cells of the stump must mobilise under the wound epidermis and begin the process of replacing lost tissues, by forming a proliferating blastema. (biomedcentral.com)
Downregulation1
- Downregulation of RCN1 promotes pyroptosis in acute myeloid leukemia cells. (stembook.org)
Pathways3
- Dedifferentiation would be represented by the marble moving uphill through the pathways it has already taken, until it settles somewhere above the most downhill location. (wikipedia.org)
- Some of the pathways that have shown interaction in dedifferentiation are MSX1, Notch 1, BMP, and Wnt/β-Catenin. (wikipedia.org)
- Within a latest meeting critique, Tanaka and Galliot described data presented by Andras Simon indicating that activation of apoptotic pathways in cultured newt myo tubes resulted within their cellularization, suggesting that these pathways may well play a part in dedifferentiation. (atpasesignaling.com)
Stump1
- Our fate mapping experiments demonstrated that the activated mesenchyme of the stump gives rise to the blastema. (unifr.ch)
Chromatin1
- Furthermore, we identified that chromatin modification is critical for redifferentiation of blastema cells. (unifr.ch)
Tissue3
- However, the markers selected to represent dedifferentiation can differ according to tissue and cell types that are being studied. (wikipedia.org)
- Dedifferentiation Dedifferentiation happens in conjunction with the libera tion of cells from their tissue matrix by protease induced histolysis. (atpasesignaling.com)
- This property involves activation of the remaining tissue at the site of injury to promote cell division, cell migration and replacement of the missing structures. (unifr.ch)
Insulin1
- Skeletal muscle stem cells confer maturing macrophages anti-inflammatory properties through insulin-like growth factor-2. (stembook.org)
Interaction1
- Increased foam cell formation results from lack of VDR-SERCA2b interaction, causing SERCA dysfunction, activation of ER stress-CaMKII-JNKp-PPARγ signaling, and induction of the scavenger receptors CD36 and SR-A1. (regenerativemedicine.net)
Mesenchymal2
- Nitric oxide-dependent immunosuppressive function of thymus-derived mesenchymal stromal/stem cells. (stembook.org)
- Leptin and NAP2 promote mesenchymal stem cell senescence through activation of PI3K/Akt pathway in patients with systemic lupus erythematosus. (stembook.org)
Stem3
- Stem Cell Res Ther. (stembook.org)
- CDK7-YAP-LDHD axis promotes D-lactate elimination and ferroptosis defense to support cancer stem cell-like properties. (stembook.org)
- Stem Cells Transl Med. (stembook.org)
Adult1
- Her lab established the cryoinjury model system for inducing myocardial infarction in adult fish. (unifr.ch)
Investigating the rege1
- We are currently investigating the regenerative capacity of specific cardiac cell populations and comparing the restorative programs of different fish species. (unifr.ch)
Pyroptosis1
- Pyroptosis, an inflammatory form of programmed cell death, is directly executed by gasdermin (GSDM) depending on its N-terminal pore-forming fragment-mediated membrane-disrupting, triggering intracellular contents release, which plays important roles in mammalian anti-infection and anti-tumor immune responses. (bvsalud.org)
Signaling pathway1
- Notch proteins are part of an intercellular signaling pathway responsible for regulating interactions between cells that are physically next to one another by binding to other notch proteins. (wikipedia.org)
Remain1
- In our modern day understanding of dedifferentiation, some controversies remain when defining the boundaries of its definition. (wikipedia.org)
Process1
- Dedifferentiation (pronounced dē-ˌdi-fə-ˌren-chē-ˈā-shən) is a transient process by which cells become less specialized and return to an earlier cell state within the same lineage. (wikipedia.org)
Issue1
- Interestingly, we discovered that LIN28, a fourth transcription issue utilised to reprogram mammalian somatic cells to pluripotency, was upregulated on all dpa. (atpasesignaling.com)
Development1
- This repressor would be able to keep cells undifferentiated during development. (wikipedia.org)
Types2
- This suggests an increase in cell potency, meaning that, following dedifferentiation, a cell may possess the ability to re-differentiate into more cell types than it did prior to dedifferentiation. (wikipedia.org)
- In the 1940s C. H. Waddington created the "Epigenetic Landscape", a diagrammatic representation of cell fate from less differentiated to more differentiated cell types. (wikipedia.org)