Decorin is an archetypal member of the small leucine-rich proteoglycan gene family and is involved in various biological functions and many signaling networks, interacting with extra-cellular matrix (ECM) components, growth factors, and receptor tyrosine kinases. (nebraska.edu)
Decorin is a small leucine rich proteoglycan initially characterized for regulating collagen fibrillogenesis. (jefferson.edu)
Human decorin is a small leucine-rich proteoglycan of 90-140 kDa that is associated with collagen fibrils in all connective tissue. (encyclopedia.pub)
Proteoglycan1
Here, we leverage biophysical microstructural cues made of hyaluronic acid (HA) loaded with the anti-fibrotic proteoglycan decorin to more robustly attenuate cardiac fibrosis after acute myocardial injury. (bvsalud.org)
Tumor microenvironment3
However, this paradigm changed upon the discovery that soluble decorin directly binds, with high affinity, to a plethora of cell surface receptor tyrosine kinases that are richly expressed by the tumor microenvironment and parenchyma. (jefferson.edu)
He is mainly interested in the study of the biology of decorin and in its antitumorigenic role in the tumor microenvironment. (iozzolab.com)
The expression of decorin mRNA in lens epithelial cells in vitro was found to decrease upon transforming growth factor (TGF)-β-2 addition and increase upon fibroblast growth factor (FGF)-2 addition. (nebraska.edu)
Vivo5
Specifically, we study the roles of decorin and perlecan, particularly the C-terminal fragment known as endorepellin, in regulating these fundamental processes by evaluating diverse in vitro systems and novel in vivo models. (jefferson.edu)
Secondly, nanoparticles were loaded with DOX and their in vitro and ex vivo/in vivo delivery was assessed, in comparison with liposomal Doxorubicin (Caelyx). (dovepress.com)
SiNPs-TZ (1:8 Hc-TZ) nanoparticles loaded with Doxorubicin (DOX-SiNPs-TZ) showed a similar DOX delivery capability than Caelyx (at 6 h p.i.), in in vitro and ex vivo assays. (dovepress.com)
Methodologically, the lab employs a wide spectrum of techniques ranging from in vitro biochemical and biophysical assays, to cell biology methods with primary cells and transformed cell lines, to light and electron microscopic techniques, to in vivo studies with genetically modified mouse models. (mcgill.ca)
While many methods have been developed to screen and evaluate the efficacy of immune cell‑mediated killing, there is a need for a more robust in vitro assay to accurately predict the in vivo behavior of therapies. (technologynetworks.com)
Collagen fibrillogenesis3
Decorin interacts with collagen via its protein core and influences collagen fibrillogenesis, thus regulating excessive bundle-like aggregation of collagen. (mattek.com)
The candidates were screened with an in vitro collagen fibrillogenesis assay and the tetrapeptide with International Nomenclature of Cosmetic Ingredients (INCI) name Tripeptide-10 Citrulline achieved the best results. (mattek.com)
Like decorin, this synthetic tetrapeptide proved, through in vitro tests, to regulate collagen fibrillogenesis and to influence the diameter of collagen fibres, making them thinner and more uniform. (mattek.com)
Assays1
While different methods exist to evaluate the efficacy of immunotherapy products, more efficient and robust in vitro assays are still required to ensure their safety and consistency. (technologynetworks.com)
Specifically, the purpose of this project was to investigate the regulation of these proteins by monocyte-derived macrophages in vitro , mimicking the hypoxic conditions of the AAA thrombus. (healthdrugpdf.com)
Co-workers and Cassatt reported that mice immunized with DbpA, a surface area lipoprotein adhesin which mediates binding towards the Flavopiridol HCl collagen-associated proteins decorin (33, 35) and it is up-regulated during an infection (13), showed incomplete security against an infection with a problem of 6 to 8 spirochetes retrieved from contaminated mouse plasma (13). (antiviralbiologic.com)
Cells9
In the present chapter, we report the development of heparin-, chondroitin sulfate-, decorin-, and poly(ε-caprolactone)-based self-assembling peptide composite scaffolds to promote re-differentiation of expanded human articular chondrocytes and induction of adipose-derived stem cells to chondrogenic commitment. (intechopen.com)
By using human lungs and cells, this new system provides a novel way to culture cells in vitro to investigate matrix contributions to lung pathology in a disease-specific manner. (atsjournals.org)
Gal-3C in vitro treatment of M2-polarized macrophage-like cells reduced their M2-phenotypic expression of arginase-1 and interleukin-10. (bvsalud.org)
Using a chromatin immunoprecipitation assay, it was noted that progesterone and dienogest directly induced the binding of the decorin promoter in the EMOsis cc/TERT cells (immortalized human ovarian epithelial cells) and CRL-4003 cells (immortalized human endometrial stromal cells). (endocrinology-journals.org)
In conclusion, decorin induced by dienogest appears to play a crucial role in suppressing endometriosis by exerting anti-proliferative effects and inducing cell cycle arrest via the production of p21 human ectopic endometrial cells and eutopic endometrial stromal cells. (endocrinology-journals.org)
Recently, we found that decorin evokes a protracted autophagic program in endothelial cells mitochondrial autophagy in tumor cells. (jefferson.edu)
The information given at AACR 2022 shows that OncoMyx's myxoma virus, which is multi-armed with IL-12 and decorin, infects and kills human multiple myeloma cells in vitro and exhibits dose-responsive effectiveness after intravenous (IV) injection in a mouse model of multiple myeloma. (vascodagamaonlinejournal.in)
In the 1960's, Hayflick observed that human cells displayed a finite lifespan when cultured in vitro. (biologiedelapeau.fr)
He later determined that most cells had a maximal capacity to proliferate in vitro of about 50 population doublings (the Hayflick limit) after which they entered what he termed cellular senescence, a process characterized by irreversible growth arrest. (biologiedelapeau.fr)
Mitochondrial autophagy1
His primary project focuses on the role of decorin in the regulation of mitochondrial autophagy (mitophagy) in triple negative breast carcinoma. (iozzolab.com)
Epithelial2
Decorin may be associated with epithelial-mesenchymal transition and PCO development in the lens. (nebraska.edu)
Our objectives were to examine the direct effects of progesterone and dienogest on the in vitro proliferation of the human ectopic endometrial epithelial and stromal cell lines, and evaluate as to how decorin contributes to this effect. (endocrinology-journals.org)
Situ1
Physiologically relevant platform for an advanced in vitro model of the vascular wall: focus on in situ fabrication and mechanical maturation. (mcgill.ca)
Inhibit2
Particularly, he is now focused on the ability of decorin to inhibit the growth of breast cancer. (iozzolab.com)
The demonstration that parasites that have the var2csa knockout gene irreversibly lose the ability to adhere to CSA ( 18 ), as well as the ability of VAR2CSA to induce antibodies that inhibit adherence of placental infective erythrocytes to CSA in vitro, strongly argue for use of VAR2CSA as a vaccine against placental malaria. (cdc.gov)
Receptor1
Upon receptor engagement, decorin briefly activates the receptor resulting in a multitude of intracellular signaling events before triggering receptor internalization and lysosomal degradation. (jefferson.edu)
Human3
To examine the effect of the natural antimicrobial peptide human β-defensin-3 (hBD-3), on the migration of a head and neck cancer cell line in vitro using microfabrication and soft-lithographic techniques. (biomedcentral.com)
Wound healing of the injured lens in mice transgenic for lens-specific human decorin was promoted by inhibiting myofibroblastic changes. (nebraska.edu)
Our results indicate that 50 ng/ml IGF-1 and 10 ng/ml TGF-β3 (in the absence of FBS) were capable of maintaining in vitro human tenocyte survival in 14-day cultures. (ox.ac.uk)
Affinity1
These sequences were engineered to generate new tetrapeptides with improved affinity that would present a decorin-like activity. (mattek.com)
The extent of tenocyte differentiation was assessed by examining cell survival, collagen synthesis, cell morphology and expression of tenocyte differentiation markers such as scleraxis (Scx), tenomodulin (Tnmd), collagen type I (Col-I) and decorin (Dcn). (ox.ac.uk)
Evaluate2
Intramyocardial injections of saline (n = 5), microrods (n = 7), decorin microrods (n = 10), and free decorin (n = 4) were performed in male rat models of ischemia-reperfusion MI to evaluate therapeutic effects on cardiac remodeling and function. (bvsalud.org)
This eBook highlights innovative tools to evaluate the potency of immunotherapies in vitro . (technologynetworks.com)
Processes1
In Iozzo's lab, he is interested in the role of decorin in cancer metastasis and lymphangiogenic processes. (iozzolab.com)
Results1
As skin ages, there is lack of functional decorin, which results in disrupted collagen fibres and in a reduction in the tensile strength of the skin. (mattek.com)
Zinc is an essential element and serum zinc deficiency can cause hair loss,Thymulin's activity on the hair follicle was recently published in an in vitro study where several thymic peptides and showed thymulin could extend the anagen phase of the hair follicle. (invigoratemedspa.com)
Model1
In a rabbit model of PVR, decorin used as an adjuvant during vitrectomy reduced the amount of fibrosis and TRD. (medscape.com)
Growth2
Decorin has been shown to be a powerful endogenous tumor repressor acting in a paracrine fashion to limit tumor growth. (endocrinology-journals.org)
Progesterone and dienogest also led to significant induced cell cycle arrest via decorin by promoting production of p21 in both cell lines in a dose-dependent manner. (endocrinology-journals.org)
Change2
Echocardiographic analysis demonstrated that rats treated with decorin microrods (5.21% ± 4.29%) exhibited significantly increased change in ejection fraction (EF) at 8 weeks post-MI compared to rats treated with saline (-4.18% ± 2.78%, p (bvsalud.org)
There is certainly biological evidence these host-adapted borreliae (HAB) change from in vitro-cultivated borreliae (IVCB) in relevant methods. (antiviralbiologic.com)
Review1
In this review, we present findings regarding the roles of decorin in the lens and ocular diseases. (nebraska.edu)