• One of these technologies envisions the use of discarded human organs as a platform for the bioengineering and regeneration of organoids for transplant purposes. (biocapan.eu)
  • As a physician scientist, Professor Orlando specializes in kidney and pancreas transplantation, and abdominal organ bioengineering and regeneration. (biocapan.eu)
  • He has been instrumental in popularizing the concept of recycling discarded human organs for tissue engineering purposes and is currently proposing the use of discarded human kidneys and pancreas as platforms for kidney and endocrine pancreas bio-engineering and regeneration. (biocapan.eu)
  • He joined Dr. Orlando's team and BioCaPan in October 2015, with the intent to specialize in regenerative medicine technologies as they are applied to organ transplantation, and ultimately develop new platforms for organ bioengineering, regeneration and repair. (biocapan.eu)
  • The goal of this review is to assess the potential of two of the most recent innovations in kidney transplant technology-the implantable bioartificial kidney (BAK) and kidney regeneration technology-in addressing the aforementioned problems related to kidney replacement for patients with ERSD. (krcp-ksn.org)
  • Two of the most recent technological advancements in this field are the implantable bioartificial kidney (BAK) and kidney regeneration technology. (krcp-ksn.org)
  • We are currently working on an automated system for organ and tissue decellularization and regeneration that includes actuator systems for mechanical conditioning and optical sensor technologies to receive feedback about the tissue quality based on imaging modalities and tissue architecture. (fau.de)
  • Contributing to organ formation and tissue regeneration, extracellular matrix (ECM) constituents provide tissue with three-dimensional (3D) structural integrity and cellular-function regulation. (bvsalud.org)
  • Gaining better insight into ECM derived from either tissue or cells that regulate 3D tissue reconstruction or organ regeneration helps us to select, produce, and implant the most suitable ECM and thus promote 3D organoid culture and tissue remodeling for in vivo regeneration. (bvsalud.org)
  • The Massachusetts researchers described their findings in the Nature Medicine article, Regeneration and orthotopic transplantation of a bioartificial lung. (regenerativemedicine.net)
  • Often, the only viable treatment for end-stage heart failure is organ transplantation. (wikipedia.org)
  • A theoretical alternative to traditional transplantation processes is the engineering of personalized bioartificial hearts. (wikipedia.org)
  • The ideal treatment option for terminally ill patients is organ transplantation. (frontiersin.org)
  • To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
  • Organ transplantation is the ultimate treatment option for patients suffering from refractory diseases. (frontiersin.org)
  • Depending on the patient's medical condition, a refractory disease patient also requires an on-time selective option, such as less invasive cellular therapy options or curative organ transplantation that can function immediately after transplantation. (frontiersin.org)
  • The ultimate goal of these investigations is to provide "organs on demand" through the development of technologies that will eventually address the two most urgent needs in organ transplantation, namely, the identification of a new and potentially inexhaustible source of organs and immunosuppression-free transplantation. (biocapan.eu)
  • Professor Orlando's training both as a transplant surgeon and regenerative medicine scientist provides a unique background for his research to transition traditional organ transplantation towards a regenerative medicine paradigm. (biocapan.eu)
  • His recent book "Regenerative Medicine Technologies Applied to Organ Transplantation", Associated Press, New York 2013, is a benchmark in transplant and regenerative medicine literature. (biocapan.eu)
  • During his training in the UK, he has developed a major interest in organ transplantation. (biocapan.eu)
  • Current opinion in organ transplantation, 24 (5). (unibas.ch)
  • So far, research, investigating the impact of sex hormones and different sex- and age-dependent sex-hormone levels on alloimmune response after solid organ transplantation is lacking. (experimental-surgery.de)
  • Liver transplantation is the only curative treatment option for end-stage liver disease, however, its use remains limited due to a shortage of suitable organs. (experimental-surgery.de)
  • In recent years, ex vivo liver machine perfusion has been introduced to liver transplantation, as a means to expand the donor organ pool. (experimental-surgery.de)
  • Ex vivo liver machine perfusion seems to be a suitable strategy to expand the donor pool for liver transplantation and holds promise as a platform for reconditioning diseased organs. (experimental-surgery.de)
  • Researchers have had many successful advances in the engineering of cardiovascular tissue and have looked towards using decellularized and recellularized cadaveric hearts in order to create a functional organ. (wikipedia.org)
  • Decellularization-recellularization involves using a cadaveric heart, removing the cellular contents while maintaining the protein matrix (decellularization), and subsequently facilitating growth of appropriate cardiovascular tissue inside the remaining matrix (recellularization). (wikipedia.org)
  • Challenges here are of course to combine the opto-biomechatronics hardware and software environment but also find biological cues and 'recipes' to mature bioartificial constructs into functional bio-tissue. (fau.de)
  • Tissue engineering provides a therapeutic method that can replace or regenerate damaged tissues and organs through the research of cells, biological scaffolds and biologically related molecules. (bvsalud.org)
  • Overall, the decellularization methodologies and tissue/cell-derived ECM as scaffolds or cellular-growth supplements used in cell propagation and differentiation for 3D tissue culture in vitro are discussed. (bvsalud.org)
  • Magnetic resonance elastography quantification of the solid-to-fluid transition of liver tissue due to decellularization " was published in the latest issue of the Journal of the Mechanical Behavior of Biomedical Materials . (experimental-surgery.de)
  • Maintenance of tissue extracellular matrix (ECM) and its biomechanical properties for tissue engineering is one of the substantial challenges in the field of decellularization and recellularization. (experimental-surgery.de)
  • Extracellular matrix (ECM) is a dynamic tissue that contributes to organ integrity and function, and its regulation of cell phenotype is a major aspect of cell biology. (atsjournals.org)
  • We report that the decellularization process effectively removes cellular and nuclear material while retaining native dimensionality and stiffness of lung tissue. (atsjournals.org)
  • Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
  • Ex situ bioengineering of bioartificial endocrine glands: a new frontier in regenerative medicine of soft tissue organs. (genescells.ru)
  • Bioartificial hearts have not been developed yet for clinical use, although the recellularization of porcine hearts with human cells opens the door to xenotransplantation. (wikipedia.org)
  • To overcome this significant crisis, researchers are investigating various approaches involving direct xenotransplantation, organoids, decellularization, and recellularization, and more recently, organ bioengineering using blastocyst complementation (BC). (frontiersin.org)
  • Preservation of the organ-specific biomatrix is crucial for successful recellularization to support cell survival, proliferation, and functionality. (experimental-surgery.de)
  • Presently, in the United States, another person is added to an organ transplant list every 10 min, 17 people die each day while waiting for donor organs, and approximately 105,800 patients are waitlisted for an organ transplant according to the health resources and services administration (HRSA). (frontiersin.org)
  • However, donor organs are in absolute shortage, and sadly, most patients die while waiting for a donor organ. (frontiersin.org)
  • However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
  • Bioartificially-engineered whole organs or heart chambers may be a feasible solution to the lack of donor organs. (savingtinyhearts.org)
  • The current standard of care for patients with end-stage renal disease (ERSD) is a kidney transplant or dialysis when a donor organ is not available. (krcp-ksn.org)
  • The growing gap between patients who require a kidney transplant and the availability of donor organs as well as the negative effects of long-term dialysis, such as infection, limited mobility, and risk of cancer development, drive the impetus to develop alternative renal replacement technology. (krcp-ksn.org)
  • This method of treatment is severely limited by donor organ availability. (krcp-ksn.org)
  • The need for donor organs in the United States is predicted to rise by 8% each year [ 2 ]. (krcp-ksn.org)
  • The current standards of care for patients with kidney failure or ESRD include full kidney transplant with a donor organ, in-center hemodialysis (HD) or peritoneal dialysis (PD), or at-home HD via an external machine. (krcp-ksn.org)
  • Both technologies address donor organ shortages as well as complications from dialysis and immunosuppressants. (krcp-ksn.org)
  • The purpose of this review is to analyze recent progress in kidney replacement technology and assess its potential impact on reducing risks associated with dialysis and donor organ kidney transplants, specifically donor shortages, renal failure, and risk of cancer. (krcp-ksn.org)
  • Historically, kidney failure following ESRD is best treated by a full kidney transplant with a donor organ. (krcp-ksn.org)
  • Given the extremely limited availability of donor organs, most patients with a failing kidney end up on dialysis, either HD or PD. (krcp-ksn.org)
  • Because minimal DNA material remains after the decellularization process, the engineered organ is biocompatible with the transplant recipient, regardless of species. (wikipedia.org)
  • In 2017, approximately 114,000 patients in the United States waited for an organ transplant ( Sykes and Sachs, 2019 ). (frontiersin.org)
  • Specific Aim-3 describes the plans to transplant the bioartificial hearts from Aim2, and study their properties in vivo. (savingtinyhearts.org)
  • In 2018 alone, over 100,000 people in the United States were on the kidney transplant waiting list, while only 21,000 organs were available for transplant [ 1 ]. (krcp-ksn.org)
  • Currently, there are 12 studies enrolled in the clinicaltrials.gov registrar, and these focus on use of ex vivo perfusion in extended criteria donors as well as declined organs. (experimental-surgery.de)
  • The preferred method to remove all cellular components from a heart is perfusion decellularization. (wikipedia.org)
  • We propose to use whole-organ decellularization to generate extracellular matrices of human-size tissues as a starting point for bioartificial organogensis. (savingtinyhearts.org)
  • For the damage and loss of tissues and organs caused by urinary system diseases, the current clinical treatment methods have limitations. (bvsalud.org)
  • The organ decellularization paradigm has opened a breach where multidisciplinary teams of biologists, clinicians, and engineers can explore new ways to engineer complex tissues. (regenerativemedicine.net)
  • This methodology is expected to allow investigation of important ECM-based hypotheses in human tissues and permits future scientific exploration in an organ- and disease-specific manner. (atsjournals.org)
  • He has been successful in developing clinically relevant models for organ bio-engineering consisting of scaffolds produced through the decellularization of porcine or human organs. (biocapan.eu)
  • We used compact magnetic resonance elastography (MRE) with 400, 500, and 600 Hz driving frequency to investigate rat liver specimens for quantification of viscoelastic property changes resulting from decellularization. (experimental-surgery.de)
  • Decellularization did not affect the integrity of microanatomy and structural composition of liver ECM but was found to be associated with increases in the relative amounts of collagen by 83-fold (37.4 ± 17.5 vs. 0.5 ± 0.01 μg/mg, p = 0.0002) and elastin by approx. (experimental-surgery.de)
  • Two-hundred-thirteen (n = 213) human whole organ liver grafts will be submitted to either NMP (n = 85) or HOPE (n = 85) directly before implantation and going to be compared to a control-group of patients (n = 43) transplanted with static cold storage preserved ECD-allografts. (experimental-surgery.de)
  • Researchers at WFUHS were the first in the world to engineer laboratory-grown organs that were successfully implanted in humans. (biocapan.eu)
  • Although far from use in a clinical setting, there have been great advances in the field of bioartificial heart generation. (wikipedia.org)
  • First, the amputated limb undergoes a process called decellularization where the cells inside are flushed out. (doctorshealthpress.com)
  • Using our refined decellularization process we generated easily obtainable TEVG with retained ECM- and mechanical quality, serving as a platform to develop small-diameter ( (experimental-surgery.de)
  • To date, no technology has achieved long-term sustainable patient-derived organ generation. (frontiersin.org)
  • In this regard, whole organ generation via the BC approach holds great promise with a ready resource (livestock) for cellular therapies and as a radical treatment option for most terminal diseases. (frontiersin.org)
  • In this review, we summarize the history of interspecies chimerism in various animal models to find hints for BC application and describe the challenges and prospects of utilizing BC for human organ generation. (frontiersin.org)
  • Genome editing on human pluripotent stem cells (hPSCs) together with the development of protocols for organ decellularization opens the door to the generation of autologous bioartificial hearts. (bvsalud.org)
  • The next generation of artificial or bio-hybrid organs may provide temporary support for patients while patient-specific regenerative solutions are prepared and implanted. (regenerativemedicine.net)
  • Generation of a Bone Organ by Human Adipose-Derived Stromal Cells Through Endochondral Ossification. (unibas.ch)
  • Currently organ supply is insufficient to meet the demand, which presents a large limitation in an end-stage treatment plan. (wikipedia.org)
  • A bioartificial heart is an engineered heart that contains the extracellular structure of a decellularized heart and cellular components from a different source. (wikipedia.org)
  • In 2014, human-sized bioartificial pig hearts were constructed. (wikipedia.org)
  • In this regard, emerging technologies of chimeric human organ production via blastocyst complementation (BC) holds great promise. (frontiersin.org)
  • Like the native organ, will the regenerated lung recruit vascular beds that will permit increasing blood flows with low resistance? (regenerativemedicine.net)
  • Engineering Small-Scale and Scaffold-Based Bone Organs via Endochondral Ossification Using Adult Progenitor Cells. (unibas.ch)
  • Also, the level of phenotypic organ recapitulation that can be achieved and is functionally adequate remains open. (regenerativemedicine.net)
  • Organs by design: can bioprinting meet self-organisation? (unibas.ch)
  • Bovine carotid arteries (BCA) were decellularized using a combined SDS (sodium dodecyl sulfate) -free mechanical-osmotic-enzymatic-detergent approach to show the feasibility of xenogenous vessel decellularization. (experimental-surgery.de)