As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis. (cellsignal.com)
Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.com)
Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. (cellsignal.com)
Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.com)
Western blot analysis of HepG2 cell lysate using P16INK4a Recombinant Rabbit Monoclonal Antibody (CDKN2A/4844R). (neobiotechnologies.com)
Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.cn)
CDK4 and CDK62
Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. (neobiotechnologies.com)
The p16 family (p15, p16, p18 and p19) binds to and inhibits the activities of CDK4 and CDK6. (prospecbio.com)
Isotype1
Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is CDK6 (E3E3Q) Rabbit mAb. (cellsignal.com)
Specificity2
At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.com)
The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. (cellsignal.com)
ELISA1
Description: A competitive ELISA for quantitative measurement of Human Anti centriole and centrosome antibody IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (cell-ess.com)
Paraffin-embedded2
Immunohistochemical analysis of paraffin-embedded normal human colon (two fields of view) using CDK6 (E3E3Q) Rabbit mAb (left) or a CDK6 Rabbit mAb (right). (cellsignal.com)
Formalin-fixed, paraffin-embedded human cervix stained with P16INK4aRecombinant Rabbit Monoclonal Antibody (CDKN2A/4844R). (neobiotechnologies.com)
Rabbit5
Western blot analysis of extracts from various cell lines using CDK6 (E3E3Q) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
using CDK6 (E3E3Q) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). (cellsignal.com)
Western blot analysis was performed using CDK6 (E3E3Q) Rabbit mAb. (cellsignal.com)
Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody. (cellsignal.com)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (cusabio.com)
Protein3
Low expression of CDK6 protein in ZR-75-1, Neuro-2a, and INS-1 cells is consistent with the predicted expression pattern. (cellsignal.com)
Immunoprecipitation of CDK6 protein from Jurkat cell extracts. (cellsignal.com)
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein. (abcam.cn)
Epitope2
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
The antibody was purified using epitope specific phosphopeptide. (abcam.com)
GAPDH1
Description: Mouse monoclonal to GAPDH (Human Specific). (sebov-detection.com)
Laboratories2
Human IgG antibody Laboratories manufactures the https://www.leebio.com/ reagents distributed by Genprice. (cell-ess.com)
Human Antibody Laboratories manufactures the names of specialty perscription human anti bodies reagents distributed by Genprice. (sebov-detection.com)
Inhibitor2
It is a specific inhibitor of cdk4/cdk6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. (neobiotechnologies.com)
Description: LEE011 Hcl is an orally available cyclin-dependent kinase (CDK) inhibitor targeting cyclin D1/CDK4 and cyclin D3/CDK6 cell cycle pathway, with potential antineoplastic activity. (cell-ess.com)
IHC-P analysis of human tonsil tissue using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
Product2
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you can trust. (cellsignal.com)
Support1
in situations where an antibody will be used to support large, long-term studies, this can be an especially critical factor. (cellsignal.com)
Analysis1
WB analysis of HeLa cell lysate using GTX33611 Cyclin D1 antibody [RM241]. (genetex.com)
Host1
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
Research1
This antibody is available for research use only and is not approved for use in diagnosis. (neobiotechnologies.com)
Potential1
In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)
Months1
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. (neobiotechnologies.com)
Reagents distributed3
Human IgG antibody Laboratories manufactures the mouse monoclonal anti-p16ink4a antibody reagents distributed by Genprice. (microbiologystudents.com)
Human IgG antibody Laboratories manufactures the rabbit human specifice cadh erin antibody reagents distributed by Genprice. (sebov-detection.com)
Iga Antibody Laboratories manufactures the iga antibody small intestine reagents distributed by Genprice. (sebov-detection.com)
Hybridoma-based2
Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. (abcam.com)
In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)
Clone2
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
Description: Anti-PSA (Prostate Specific Antigen) Rabbit Monoclonal Antibody, Clone#RM323 tested in WB, IHC. (sebov-detection.com)
Rabbit3
Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody. (cellsignal.com)
Description: A sandwich ELISA for quantitative measurement of Rabbit Granulocyte Specific Antinuclear Antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (sebov-detection.com)
Description: A sandwich ELISA for quantitative measurement of Rabbit anti-calmodulin specific antibody,CaM-ab in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (sebov-detection.com)
Polyclonal1
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
Immunogen1
Based on the immunogen sequence, we do not expect the antibody to cross-react with other CDK family members. (abcam.com)
Azide2
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. (abcam.co.jp)
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. (avivasysbio.com)
Catalog1
The following product was used in this experiment: Cyclin D1 Monoclonal Antibody (3D8) from Thermo Fisher Scientific, catalog # MA5-15650, RRID AB_10978307. (thermofisher.com)
Consistent1
The small intestine tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. (sebov-detection.com)
Expression1
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
Cell2
Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. (thermofisher.com)
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. (abcam.co.jp)
Region1
What buffer format is "SFRS7 Antibody - middle region (ARP56232_P050)" provided in? (avivasysbio.com)
Long term1
in situations where an antibody will be used to support large, long-term studies, this can be an especially critical factor. (cellsignal.com)