• Imidazo[1,2-b]pyrazole-7-carboxamides induce apoptosis in human leukemia cells at nanomolar concentrations. (celljournal.org)
  • Riedl S, Leber R, Rinner B, Schaider H, Lohner K, Zweytick D. Human lactoferricin derived di-peptides deploying loop structures induce apoptosis specifically in cancer cells through targeting membranous phosphatidylserine. (celljournal.org)
  • Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
  • Real-life exposure to Fusarium toxins deoxynivalenol and zearalenone triggers apoptosis and activates NLRP3 inflammasome in bovine primary theca cells. (inrae.fr)
  • Natural products have been shown to induce the expression of apoptosis regulators that are blocked in cancer cells. (afpm.org.my)
  • Altogether, the results of the present study demonstrate that IR-SF may induce apoptosis in human colorectal carcinoma cells through activation of ROS-mediated apoptotic pathways. (afpm.org.my)
  • The cytotoxic effect of complexes between α-lactalbumin and linoleic acid, vaccenic acid, or oleic acid was further investigated using flow cytometry and found to induce cell death resembling apoptosis on Jurkat cells. (teagasc.ie)
  • Here, we have demonstrated that branched (25 kDa) and linear (750 kDa) PEI can both induce membrane damage and initiate apoptosis in three clinically relevant human cell lines (Jurkat T cells, umbilical vein endothelial cells, and THLE3 hepatocyte-like cells). (houstonmethodist.org)
  • The colony-forming ability of the cells was evaluated using plate cloning assay, and cell cycle changes and cell apoptosis were analyzed with flow cytometry. (bvsalud.org)
  • We have excluded apoptosis as main reason for tumor cell death after asparaginase treatment because multi resistant Jurkat/A4 cells have been asparaginase sensitive. (msk.ru)
  • Apoptosis induction potential of each compound determined by mitochondrial membrane potential analysis (Rho123), cleaved caspase-3 expression analysis by flow cytometry and immunofluorescent staining and cell morphology analysis by giemza, hematoxylin and eosin and Papanicolaou protocols. (ijpsonline.com)
  • In the cell morphology analyzes, chromatin condensation and marginalization, changes in the cell membrane, ghost cells and apoptotic bodies were observed as evidence of apoptosis formation. (ijpsonline.com)
  • The inhibition of cell proliferation and apoptosis on MDA-MB231 and MCF-7 cell lines occurred via the mitochondrial pathway and induction of oxidative damage to DNA through Bax/Bcl-2 modulation, decreased levels of procaspase-8, -9, -3, increased levels of cleaved caspase-3 and ROS, and also cell cycle arrest at S-phase (Jamali et al. (dieselok.md)
  • Several years later, Kerr and co-workers 2 defined the morphological changes in cells undergoing this process, also known as apoptosis. (nature.com)
  • We discuss here the contribution of glycan-lectin interactions to the initiation, execution and resolution of apoptosis and their emerging roles in other cell death programs including autophagy. (nature.com)
  • Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. (hindawi.com)
  • Target cells in antibody-dependent cellular cytotoxicity (ADCC) or antibody-dependent cellular phagocytosis (ADCP) , either with peripheral blood mononuclear cells, Natural Killer cells, or Jurkat-derived reporter T cells. (invivogen.com)
  • RTX's mechanism of action includes complement‑dependent cytotoxicity (CDC) and antibody‑dependent cellular cytotoxicity (ADCC). (spandidos-publications.com)
  • The mechanisms of action by which RTX kills FL cells include complement-dependent cytotoxicity (CDC) ( 3 ), antibody-dependent cellular cytotoxicity (ADCC) ( 4 , 5 )-herein referred to as 'effector function' mechanisms-as well as CD20 signaling effects ( 6 ). (spandidos-publications.com)
  • We further evaluated a subset of samples with strong binding responses for neutralizing, antibody-dependent cellular phagocytosis (ADCP), and antibody-dependent cellular cytotoxicity (ADCC) responses. (cdc.gov)
  • We have a dedicated team that provides support for ex vivo pharmacodynamic (PD) and pharmacokinetic (PK) studies, and in vitro primary and cultured cell-based assays. (biocytogen.com)
  • T-cell receptor (TCR) gene transfer is an attractive strategy for rapid in vitro generation of high numbers of antigen specific T cells. (haematologica.org)
  • The results showed that replacement of 25% and 50% 7 α-hydroxycholesterol for cholesterol in iLNPs improved mRNA delivery to primary human T cells in vitro by 1.8-fold and twofold, respectively.C14-4 is one of the ionizable lipids to efficiently deliver mRNA to Jurkat cells or primary human T cells. (dcchemicals.com)
  • In the current study, a combined in vivo and in vitro approach has been employed to investigate the mechanisms of quartz-induced proinflammatory activation of lung epithelial cells. (biomedcentral.com)
  • In vitro , the direct, particle-mediated effect on proinflammatory signalling in a rat lung epithelial (RLE) cell line was compared to the indirect, macrophage product-mediated effect. (biomedcentral.com)
  • The high potential of the PEGylated comb-like polymeric nanocarrier (PNC) in delivering both traditional and experimental drugs to tumor cells in vitro and in vivo has been demonstrated previously. (biomedcentral.com)
  • The reduced toxicity of this PNC in combination with its improved bioavailability and previously detected capability to enhance cytotoxicity toward tumor cells in vitro and potential tumor treatment effects in vivo suggests its potential as a safe drug delivery platform for treating various diseases, especially cancer. (biomedcentral.com)
  • Moreover, this is the first paper to clearly establish that a CD56 bright CD3 - CD16 - perforin- NK reg population associates with a lack of cGvHD and has several unique characteristics, including the suppression of helper T-cell function in vitro. (haematologica.org)
  • We have generated a high affinity and selective humanized monoclonal IgG4 antibody, TSR-033, which binds human LAG-3 and serves as a functional antagonist, enhancing in vitro T-cell activation both in mixed lymphocyte reactions and staphylococcal enterotoxin B-driven stimulation assays. (aacrjournals.org)
  • e Relationship between TLR2 or TLR4 appearance and percent of B7-H1-positive cells (Pearsons relationship) Appearance.Furthermore, both ligands activate or inhibit T cells in AML, based on their degree of appearance, which may be modified in vitro and in vivo simply by chemotherapy [18]. (memorial2014.com)
  • Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
  • In each of these diseases, genes that play a role in the proliferation or activation of CD8+ T cells have been found to be affected by epigenetic modifications. (frontiersin.org)
  • Data obtained, shows that IR-SF inhibited cell proliferation and induced typical apoptotic changes in the overall morphology of all the CRC cell lines tested. (afpm.org.my)
  • Potential effects of forced CD20 expression on T-cell function were investigated by comparing CD20- and mock-transduced cytomegalovirus (CMV) specific T cells for cytolysis, cytokine release and proliferation. (haematologica.org)
  • A specific PLEKHA4 siRNA was transfected in glioma U251 and T98G cells, and the changes in cell proliferation ability were assessed by real-time cell analysis technology and Edu experiment. (bvsalud.org)
  • In cell coculture assays, ICOVIR-15K-cBiTE-mediated oncolysis resulted in robust T-cell activation, proliferation, and bystander cell-mediated cytotoxicity. (aacrjournals.org)
  • IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. (thermofisher.com)
  • Kelly and SH-SY5Y neuroblastoma cells also exhibited a reduced proliferation rate after exposure to carvacrol (Kocal and Pakdemirli, 2020). (dieselok.md)
  • Understanding the function of lectin-glycan recognition systems in cell death will facilitate the implementation of novel therapeutic strategies aimed at controlling unbalanced cell proliferation and survival in several pathologic conditions. (nature.com)
  • Analogous studies in a murine syngeneic tumor model using surrogate antibodies demonstrated significant synergy between LAG-3 and PD-1 blockade-combination treatment led to a marked improvement in therapeutic efficacy, increased T-cell proliferation, IFNγ production, and elicited durable immunologic memory upon tumor rechallenge. (aacrjournals.org)
  • CD20-transduced T cells with and without co-transferred T-cell receptor were efficiently eliminated by complement dependent cytotoxicity induced by therapeutic anti-CD20 antibody rituximab. (haematologica.org)
  • 11 - 14 Upon ligation of CD20, RTX triggers various effector mechanisms, including complement-dependent cytotoxicity (CDC). (haematologica.org)
  • In the present study, the anticancer activity of pleurocidin-like peptide WF3 isoform X2 (AMP-WF3), from the Poecilia Mexicana fish, against leukemic cell line Jurkat was evaluated, and the cytotoxicity compared with the effects on normal cells, including peripheral blood mononuclear cells (PBMCs) and human dermal fibroblast (HDF) cells. (celljournal.org)
  • We have studied dose- and time-dependent antitumor and cytotoxic effects of Erwinia carotovora L-asparaginase (ECAR LANS) and Escherichia coli L-asparaginase (MEDAC) on human leukemic cells and human and animal solid tumor cells. (msk.ru)
  • Evaluation of leukemic cell number after treatment with L-asparaginases for 24, 48 and 72 h demonstrated that asparagine deficiency did not kill cells but stopped normal cell division and had no effect on protein and DNA synthesis. (msk.ru)
  • Cytofluorometric study of solid and leukemic cells demonstrated that the treatment with L-asparaginase for 72 h did not change cell cycle phase distribution and did not increase the number of apoptotic cells. (msk.ru)
  • Additionally, NK reg cells do not kill leukemic cells. (haematologica.org)
  • The inducibility of B7-H1 appearance by TLR or IFN- ligands shows that several stimuli, either produced through the immune system response against leukemia cells or released by infectious microorganisms, could secure leukemic cells from T cells. (memorial2014.com)
  • Appearance also increased in Jurkat and U937 cells on 24-h incubation in 500?IU/ml IFN-, suggesting that leukemic cells could exhibit B7-H1 below appropriate conditions. (memorial2014.com)
  • Open up in another window Fig.?1 TLR and B7-H1 expression in leukemic cell lines and blast cells from AML. (memorial2014.com)
  • a Stream cytometry evaluation of B7-H1 appearance in myeloid leukemic cell lines (LAMA-84, HL60, K562, U937, KG1a, THP-1) and lymphoid lines (Raji, Jurkat) with or without incubation with 500?IU/ml IFN- for 24?h. (memorial2014.com)
  • In this study, naïve CD4 T cells were incubated with mature dendritic cells and treated with a Nivolumab analog. (biocytogen.com)
  • Various immune-suppressive cell populations appear to associate with suppression of cGvHD, including T reg cells, B reg cells, M2 macrophages, dendritic cells, and CD56 bright natural killer (NK) cells. (haematologica.org)
  • SARS-CoV-2 spike and nucleocapsid proteins fail to activate human dendritic cells or "" T cells. (tcd.ie)
  • Humanized B-hCD40 mice were treated with different doses of an anti-human CD40 antibody, and the percentage of receptor occupancy on B cells isolated from peripheral blood mononuclear cells (PBMCs) was assessed by flow cytometry. (biocytogen.com)
  • Peripheral blood mononuclear cells (PBMC) were collected from healthy donors after approval by the LUMC institutional review board and informed consent according to the Declaration of Helsinki. (haematologica.org)
  • This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
  • MR1-restricted TCRs with specificity for tumor cells have been described, raising interest in their potential therapeutic application for cancer treatment. (bmj.com)
  • Hematopoietic stem cell transplantation (HSCT) is an important therapeutic option for patients with non-malignant diseases as well as high-risk and refractory hematopoietic malignancies. (haematologica.org)
  • This approach set the basis for therapeutic strategies aimed at eliminating aberrantly glycosylated cancer cells. (nature.com)
  • Cytotoxicity activities of the crude ethanol extracts from Chamaecostus subsessilis and Chamaecostus cuspidatus and the fractions obtained by liquid-liquid partition were observed when these species were assayed against a panel of six human cancer cell lineages (HL60, Jurkat, MDA-MB231, MCF-7, HCT, THP-1) and normal (Vero) cells using the MTT colorimetric assay. (iomcworld.com)
  • Raji-APC cells stably overexpressing an IC molecule (e.g. hPD-L1) are part of InvivoGen's anti-IC assay (Bio-IC™) . (invivogen.com)
  • The mixed lymphocyte reaction (MLR) assay typically involves co-incubation of immune cell subtypes to trigger their activation. (biocytogen.com)
  • In addition to potent and selective BTK enzyme and cellular activity, rilzabrutinib inhibited activation and inflammatory activities of B cells and innate cells such as macrophages, basophils, mast cells, and neutrophils, without cell death (in human and rodent assay systems). (aai.org)
  • We designed and synthesized novel PNP inhibitors based on acyclic nucleoside phosphonates, where the most potent compounds exhibited good potency in enzymatic assay (hPNP) but also significant cytotoxic effects in several leukemia cell lines (CCRF-CEM, MOLT-4, Jurkat). (biospot.eu)
  • The tested compounds proved to be non-toxic in any of the cell lines tested under standard assay conditions, however, co-incubation of the cells with the inhibitors and 10 µM deoxyguanosine greatly enhanced cytotoxicity of many compounds. (biospot.eu)
  • Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
  • This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
  • Compounds (C1-8 and doxorubicin) were placed to 96-well culture plate in triplicate order at doses of 1, 10, 100, 1000 μM and then K562 cells were seeded into the wells at the dose of 105/ml. (ijpsonline.com)
  • Mitochondrial membrane potential was found at low levels in cells treated with Schiff base compounds and doxorubicin. (ijpsonline.com)
  • The lack of targeted immunotherapeutic approaches for T cell malignancies results in part from potential risks associated with targeting broadly expressed T cell markers, namely T cell depletion and clinically significant immune compromise. (mdpi.com)
  • Peripheral T cell lymphomas (PTCLs) are generally chemotherapy resistant and have a poor prognosis. (mdpi.com)
  • The approval (Japan in 2017) of forodesine (ImmH), a transition-state analogue inhibitor of human PNP for treatment of peripheral T-cell lymphoma, validated PNP as druggable target with potential clinical applications, namely for treatment of T-cell leukemias. (biospot.eu)
  • Leukemia is a blood cancer that develops in the hematopoietic system and presents in the bone marrow with or without peripheral blood cell involvement[ 1 , 2 ]. (ijpsonline.com)
  • Era of cytotoxic T cells T cells in the peripheral bloodstream of a wholesome donor had been isolated utilizing a Skillet T Cell Isolation Package (Miltenyi Biotec) and cultured in RPMI 1640 (Lifestyle Technology) supplemented with 10% fetal leg serum, 100?IU/ml penicillin, 100?mg/ml streptomycin, 2?mM l-glutamine, 50?M -mercaptoethanol and 20?IU/ml interleukin 2 (PeproTech, Rocky Hill, USA). (memorial2014.com)
  • In contrast, knockdown of BRD4 but not of BRD2 impairs NK cell cytolytic responses, suggesting BRD4 as critical regulator of NK cell mediated tumor cell elimination. (frontiersin.org)
  • This is supported by pharmacological targeting where the first-generation pan-BET bromodomain inhibitor JQ1(+) displays anti-inflammatory effects and inhibit tumor cell eradication, while the novel bivalent BET bromodomain inhibitor AZD5153, which shows differential activity towards BET family members, does not. (frontiersin.org)
  • Tumor cell invasion at the metastatic site is effectively reduced upon CXCR4 silencing (Fig. 7B), similar to the antagonist IT1t [3] . (medchemexpress.com)
  • The present study demonstrated that CA125 could bind to RTX and reduce its tumor cell killing activity. (spandidos-publications.com)
  • We have demonstrated that the synthesized PNC possesses weak toxic effects toward human leukemia cells (HL-60 and Jurkat lines), as well as toward hepatocellular (HepG2), colon (HCT116) and breast (MCF-7) tumor cell lines. (biomedcentral.com)
  • Fusobacterium nucleatum (Fn) is an oral anaerobic bacterium that has recently been found to colonize on the surface of colorectal cancer cells in humans, and its degree of enrichment is highly negatively correlated with the prognosis of tumor treatment. (bvsalud.org)
  • Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)
  • kamtschaticus has shown potent cytotoxicity against Jurkat T cells. (wikipedia.org)
  • Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. (nature.com)
  • Here, we generate dromedary camel nanobodies targeting B7-H3 and demonstrate that CAR-T cells, based on the nanobodies recognizing the IgC but not IgV domain, had potent antitumour activity against large tumors in female mice. (nature.com)
  • The most potent nanobody-based CAR-T cells show inhibition of large tumor xenografts in mice with rigorous T-cell signaling and significant T-cell infiltration into the tumor. (nature.com)
  • Compared with electroporated CAR T cells, the CAR T cells engineered via C14-4 iLNPs showed potent cancer-killing activity when they were cocultured with Nalm-6 acute lymphoblastic leukemia cells. (dcchemicals.com)
  • How do C-type lectins tailor adaptive immunity following phagocytosis of apoptotic cells? (nature.com)
  • Methods The MR1-restricted T cell clone, MC.7.G5 (7G5.TCRT), with reported cancer specificity and pan-cancer activity, 1 was cloned and expressed via lentiviral transduction in Jurkat E6.1 TCRαβ- β2M- CD8+ NF-κB:CFP NFAT:eGFP AP-1:mCherry cells or in human donor T cells (with or without CD8 or after knockout of the TRBC1 and 2 genes with CRISPR Cas9). (bmj.com)
  • In contrast, transduction into human TCRα/β-positive T-cells revealed that mispairing is largely reduced. (oncotarget.com)
  • PI3k belongs to a family of lipid kinases, involved in extracellular signals transduction and cell growth promotion (Wullschleger et al. (dieselok.md)
  • In particular, NK cells mediate their cytolytic function through the engagement of activating receptors, such as NKG2D, DNAM-1, NKp30, NKp46, and NKp44 ( 3 , 4 ), or following pro-inflammatory cytokine stimulation ( 5 ). (frontiersin.org)
  • The activation, differentiation, and development of CD8+ T cells is accompanied by large-scale changes in the coordinated expression of numerous inflammatory cytokines, transcription factors (TFs) and chemokines that are correlated with their survival, effector function, and self-renewal ( 7 ). (frontiersin.org)
  • Historically, these cells have proven to be convenient target cells in studies assessing the effector functions mediated by monoclonal antibodies (mAbs). (invivogen.com)
  • In this study, anticancer properties of heterocyclic salicylaldimines and their Cu(II) complexes on K562 human chronic myelogenous leukemia cell line were investigated. (ijpsonline.com)
  • The first-in-human testing of scFv-based B7-H3 CAR-T cells has shown antitumor response against anaplastic meningioma and childhood diffuse intrinsic pontine glioma without severe side effects 16 , 17 . (nature.com)
  • Although human CD20 has previously been described as functional selection marker and suicide gene after retroviral transfer to T lymphocytes, 9 , 10 the specificities of these T cells were unknown, and therefore a potential effect of CD20 on antigen-specific T-cell functions could not be investigated. (haematologica.org)
  • T-cell clones were stimulated every 10-14 days with irradiated allogeneic PBMC and phyto-hemagglutinin in IMDM with 5% human serum (HS), 5% FBS and 100 IU/mL IL-2 as previously described. (haematologica.org)
  • All complexes were cytotoxic to human promyelocytic leukemia-derived (HL-60) cells but to different degrees depending on the fatty acid. (teagasc.ie)
  • In a human Jurkat-76 T-cell line lacking endogenous TCRs, surface expression and function of a scTCR could be reconstituted by any cointroduced TCRα-chain indicating mispairing to take place on a molecular basis. (oncotarget.com)
  • 2002). The Mechanism of Carvacrol-Evoked [Ca2+]i Rises and Non-ca2+-triggered Cell Death in OC2 Human Oral Cancer Cells. (dieselok.md)
  • Lymphocyte activation gene-3 (LAG-3) is a coinhibitory receptor associated with impaired T-cell function and is frequently coexpressed with programmed cell death protein-1 (PD-1) in the context of human cancers. (aacrjournals.org)
  • Previous study has shown that kenaf seed oil extracted by SFE (KSO-SFE) is cytotoxic towards human cervical cancer cell line [ 7 ]. (hindawi.com)
  • nevertheless, its appearance in human cancers cell lines provides were modest. (memorial2014.com)
  • Raji lymphoblast-like cells were established from a Burkitt lymphoma patient. (invivogen.com)
  • We viewed B7-H1 appearance in a number of myeloid lines (U937, K562, KG1a, HL60, THP-1) and lymphoid lines (Raji, Jurkat). (memorial2014.com)
  • The cytotoxic effects in the HL60, Jurkat and THP-1 lineages were mediated via an apoptotic mechanism when treated with crude ethanol extracts from these species. (iomcworld.com)
  • CD8+ T cells, also called cytotoxic T lymphocytes (CTL), are one subtype of T cell ( 6 ), characterized by robust production of interferon (IFN)-γ and cytolytic activities via perforin (PRF)/granzymes (GZM) or Fas mechanisms to kill target cells ( 7 ). (frontiersin.org)
  • The molecular mechanisms mediating drug resistance in cancerous cells include increased function of enzymes that render drugs ineffective, mutations in drug targets, and abnormal efflux pump activity (4). (whatisepigenetics.com)
  • Two decades later, pioneering studies suggested that lectin-like molecules constitutively expressed on the surface of macrophages can selectively recognize changes on glycans decorating the surface of apoptotic thymocytes, 4 , 5 although these studies likewise did not provide substantial insight into the mechanisms by which lectin-glycan interactions regulate cell death. (nature.com)
  • Although they display low to moderate toxicity in cells in culture, the underlying mechanisms of surfactant-mediated cytotoxicity are poorly investigated. (umw.edu)
  • Single-cell transcriptome RNA sequencing coupled with functional T-cell proteomics analysis uncovers the top-upregulated genes that might be critical for the persistence of polyfunctional CAR-T cells in mice. (nature.com)
  • was carried out through the use of siRNA/ASO or CRISPR/Cas9 functional program to knockdown or knockout TANCR, and verified that silencing of (S)-3-Hydroxyisobutyric acid TANCR inhibits Path manifestation in several types of cells, including HEK293T cells, Jurkat cells, and major T cells. (vaggi.org)
  • Functional activity of 7G5.TCRT was demonstrated by cytotoxicity utilizing the xCELLigence analyzer and by ELISA for cytokine release after co-culture with cancer cell lines with or without loading of known MR1 ligands. (bmj.com)
  • Results The TCR cloned from MC.7.G5 retains MR1 restricted functional cytotoxicity as 7G5.TCRT. (bmj.com)
  • Advances in research techniques since then have shown the mitochondria in cancer cells to be functional across a range of tumour types. (researchgate.net)
  • The emergence of functional studies on animal lectins during the 1990s has provided the appropriate framework to better understand their roles in cell death. (nature.com)
  • Arising from the transformation of epithelial cells in the colon and/or rectum into malignant cells, the foundation of CRC pathogenesis lies in the progressive accumulation of mutations in oncogenes and tumor-suppressor genes, such as APC and KRAS. (afpm.org.my)
  • These changes are thought to lead to tumor-microenvironment interactions via factors secreted by stromal or epithelial cells in response to lymphoma cell-produced cytokines ( 7 ). (spandidos-publications.com)
  • High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
  • Activation of the classical NF-κB pathway in macrophages and type II pneumocytes was indicated by enhanced immunostaining of phospho-IκBα in these specific lung cell types. (biomedcentral.com)
  • Supernatant from quartz-treated macrophages rapidly activated the NF-κB signalling pathway in RLE cells and markedly induced iNOS mRNA expression up to 2000-fold compared to non-treated control cells. (biomedcentral.com)
  • Quilty F, Freeley M, Gargan S, Gilmer J, Long A., Deoxycholic acid induces proinflammatory cytokine production by model oesophageal cells via lipid rafts. (tcd.ie)
  • Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • At that time, glycobiology, which is the study of carbohydrates and their recognition by motif-specific carbohydrate-binding proteins or lectins, lagged far behind the studies that defined the structural and cellular biology of cell death. (nature.com)
  • 10 Galectins can function inside the cells by modulating signaling pathways, 11 although they also act extracellularly by establishing multivalent interactions with cell surface glycans and delivering signals that lead to disruption of cellular homeostasis. (nature.com)
  • Flow cytometry verified that the apoptotic activity of AMP-WF3 on Jurkat cells was significantly higher than that of HDF and PBMCs. (celljournal.org)
  • Possibility of the use of flow cytometry and spectrofluorimetry analysis for investigation mitochondria and plasma membrane polarization in myometrium cell suspension using potential-sensitive probe 3,3′-dihexyloxacarbocyanine [DiOC 6 (3)] has been demonstrated. (ukrbiochemjournal.org)
  • PEI is also cytotoxic, but the molecular basis of its cytotoxicity is poorly understood. (houstonmethodist.org)
  • These genes are involved in T cell regulation, including interferons, interleukin (IL),tumor necrosis factor (TNF), as well as linker for activation of T cells (LAT), cytotoxic T-lymphocyte-associated antigen 4 (CTLA4), and adapter proteins. (frontiersin.org)
  • Antigen-presenting cells (APCs) in screening bioassays for new molecules, including mAbs, interfering with immune checkpoints . (invivogen.com)
  • The use of CD20 as suicide gene was investigated in CMV specific T cells and in T cells genetically modified with an antigen specific T-cell receptor. (haematologica.org)
  • CD20 is a B-cell-specific antigen expressed on malignant FL cells. (spandidos-publications.com)
  • Jurkat cells and major T cells had been cultured in RPMI moderate (Invitrogen, Carlsbad, CA, USA) with 10% FBS. (vaggi.org)
  • Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)
  • We determined the sensitivity of tumor cells to L-asparaginases, as well the effect L-asparaginases on cell growth rate, protein and DNA synthesis per se and with addition of different cytostatics. (msk.ru)
  • C14-4 (C14-494,Lipid B-4,Lipid B4) is a novel ionizable lipid with the highest T-cell transfection efficiency and low cytotoxicity.The C14-4 ionizable lipid has been explored for CAR-T therapy.To screen the excellent formulations for mRNA delivery, a lipid library of 24 ionizable lipids was constructed to make iLNPs, which were used to deliver luciferase mRNA into Jurkat cells. (dcchemicals.com)
  • peptide] complex, providing a cognate TCR stimulation on Jurkat-Lucia™ TCR-derived cells. (invivogen.com)
  • Biochemical and haematological analysis revealed that H-Pb was associated with low body weight and feed efficiency, low total protein levels (p ≤ 0.05), high blood lead (Pb-B) levels (p ≤ 0.001), low ALAD (δ-aminolevulinate dehydratase) activity (p ≤ 0.0001), high creatinine (p ≤ 0.0001) and blood urea nitrogen (BUN) (p ≤ 0.01) levels, elevated RBC and WBC counts, reduced haemoglobin and blood cell indices compared to control. (afpm.org.my)
  • To knock out TANCR in Jurkat T and cells cells, a vector including TANCR help RNAs and plasmid including cas9 protein had been packed in HEK293T cells respectively. (vaggi.org)
  • Genome-wide CRISPR-Cas9 screening reveals ubiquitous T cell cancer targeting via the monomorphic MHC class I-related protein MR1. (bmj.com)
  • Cancer cells often maintain high basal level of ROS and are vulnerable to any further increase in ROS levels beyond a certain protective threshold. (degruyter.com)
  • This is because cancer cells maintain high basal level of ROS, thus they are vulnerable to any further increase in ROS (Fig. 1 ). (degruyter.com)
  • Some cationic anti-microbial peptides show a wide range of cytotoxic action versus malignant cells, which may lead to developing a novel group of antitumor medications. (celljournal.org)
  • 1 , 2 The anti-tumor effect of DLI is mediated by donor T cells recognizing allo-antigens on the malignant cells of the recipient. (haematologica.org)
  • Donor T cells may also induce GvHD due to recognition of allo-antigens on non-malignant tissues of the patient. (haematologica.org)
  • Given the important role of both cytokine-mediated inflammatory microenvironment and cytolytic NK cell activities in immune-oncology therapies, our findings present a compelling argument for further clinical investigation. (frontiersin.org)
  • With further investigation we may decipher the mechanism of NK reg suppression and operationalize expansion of NK reg cells associated with cGvHD suppression. (haematologica.org)
  • Following activation, NK cells mediate killing of target cells through two major pathways that require direct contact between NK cells and their target cells ( 6 ). (frontiersin.org)
  • Bruton tyrosine kinase (BTK) is expressed in B cells and innate immune cells, acting as an essential signaling element in multiple immune cell pathways. (aai.org)
  • Fluorescent staining assays revealed characteristic nuclear condensation, and marked decrease in mitochondrial outer membrane potential in treated cells. (afpm.org.my)
  • In the initiation and progression of pulmonary inflammation, macrophages have classically been considered as a crucial cell type. (biomedcentral.com)
  • Adaptive immune cells, including B and T cells, have been demonstrated to be primary contributors to the overactive immune response and overproduction of antibodies. (frontiersin.org)
  • Movement cytometry Cells had been clogged with 5% BSA diluted in PBS for 20?min and stained with the next surface area antibodies: anti-CD3, (S)-3-Hydroxyisobutyric acid anti-TCR , and anti-TRAIL for 30?min. (vaggi.org)
  • In this study, we tested the hypothesis that tumor-infiltrating T cells could be more effectively activated and redirected by oncolytic adenoviruses that were armed with bispecific T-cell-engager (BiTE) antibodies. (aacrjournals.org)
  • and a single-clone working cell bank for late-stage and pivotal clinical trials were successively adopted. (bvsalud.org)
  • Despite the existence of a highly immunosuppressive tumor environment, adenovirus-infected cells can nonetheless be efficiently cleared by infiltrating cytotoxic T lymphocytes (CTL) without compromising tumor burden. (aacrjournals.org)
  • IC50)=50 μM], while normal cells (PBMCs and HDF) were less susceptible. (celljournal.org)
  • In contrast, the changes in P21, P53, and BCL-2 genes differed in PBMCs and HDF cells. (celljournal.org)
  • In HDF cells, simultaneous increase of P21, P53, and BCL-2, and in PBMCs, only the increase of BCL-2 was observed. (celljournal.org)
  • T cells had been finally purified with an Anti-TCR gamma delta Micro-Bead Package (Miltenyi Biotec, Germany) from IPP treated PBMCs based on the producers instructions. (vaggi.org)
  • MEK inhibitors, including AZD6244 and UO126, reduced B7-H1 appearance and restored CTL-mediated lysis of blast cells. (memorial2014.com)
  • Their function is regulated by the activation of a number of activating and inhibitory receptors that bind to specific ligands expressed on the surface of target cells. (frontiersin.org)
  • Conclusions These data demonstrate that healthy individuals can harbor T cells reactive to MR1 variants displaying self-ligands expressed in cancer and benign tissues. (bmj.com)
  • Appearance of TLR2 and TLR4 ligands or IFN- induced by B7-H1 was discovered to safeguard AML cells from CTL-mediated lysis. (memorial2014.com)
  • They used the inhibition of proprotein convertases ( PCSK1 ) in T cells and programmed death-ligand 1 ( CD274 ) in cancer cells. (emerginginvestigators.org)
  • Anna Sannino, Olga Zeni, Stefania Romeo , Rita Massa, Maria Rosaria Scarfì, Adverse and beneficial effects in chinese hamster lung fibroblast cells following radiofrequency exposure, Bioelectromagnetics 38(4): 245-254, 2017. (cnr.it)
  • The HL-60 cell line was only exemption. (msk.ru)
  • doi:10.7287/peerj.9626v0.1/reviews/1, Soković, M., Glamoclija, J., and Ciric, A. Thymol also caused a decrease in cell viability of prostate cancer (PC-3 cell line), and provoked cytotoxic effects (Pathania et al. (dieselok.md)
  • Natural killer (NK) cells are innate lymphocytes that play a pivotal role in the immune surveillance and elimination of transformed or virally infected cells. (frontiersin.org)
  • Natural killer cells are cytolytic lymphocytes belonging to the innate immune system and are involved in anti-viral and anti-tumor responses ( 1 ) and are recognized as major players in immune-mediated anti-tumor therapies ( 2 ). (frontiersin.org)
  • Therefore, this study determined the cytotoxicity of KSO-SFE on colon cancer cells. (hindawi.com)
  • A structure-activity relationship study (SAR) indicates that steric effectsof some substituents on the aromatic ring lead to reduce the cytotoxicity of styryllactone derivatives/analogues. (uitm.edu.my)
  • In this experimental study, cells were treated with various dosages of AMP-WF3 for 24 hours. (celljournal.org)
  • In the present study, we demonstrate that B7-H3 CAR-T cells targeting the IgC domain are more active than those targeting the IgV domain in pancreatic ductal adenocarcinoma (PDAC) and neuroblastoma (NB) mouse preclinical models. (nature.com)
  • In this study, we investigated the use of CD20 as safety switch after retroviral transfer to T cells with different cytomegalovirus (CMV) specificities, and conclude that CD20 may be broadly applicable as safeguard in adoptive T-cell therapy. (haematologica.org)
  • This study suggests a novel strategy to reactivate T cells. (emerginginvestigators.org)
  • Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
  • ROS-modulation in normal vs. cancer cell. (degruyter.com)
  • The aim of adoptive T-cell therapy of cancer is to selectively confer immunity against tumor cells. (haematologica.org)
  • 1 Recognition is restricted to cells expressing a SNV of MR1 (MR1*04) and is not cancer specific. (bmj.com)
  • 7G5.TCRT and 7G5-like TCRT cells react to both cancer and healthy cells endogenously expressing MR1*04 SNVs, which encode R9H and H17R substitutions. (bmj.com)
  • Immunotherapy of cancer envisions the adoptive transfer of T-cells genetically engineered with tumor-specific heterodimeric α/β T-cell receptors (TCRα/β). (oncotarget.com)
  • Influential research by Warburg and Cori in the 1920s ignited interest in how cancer cells' energy generation is different from that of normal cells. (researchgate.net)
  • They observed high glucose consumption and large amounts of lactate excretion from cancer cells compared with normal cells, which oxidised glucose using mitochondria. (researchgate.net)
  • It was therefore assumed that cancer cells were generating energy using glycolysis rather than mitochondrial oxidative phosphorylation, and that the mitochondria were dysfunctional. (researchgate.net)
  • This review will discuss the metabolic reprogramming of cancer, possible explanations for the high glucose consumption in cancer cells observed by Warburg, and suggest key experimental practices we should consider when studying the metabolism of cancer. (researchgate.net)
  • Since cancer cells inhibit T-cell activity, the authors investigated a method to reverse T-cell disfunction with gene therapy, so that the T-cells would become effective once again in fighting cancer cells. (emerginginvestigators.org)
  • Cell cycle involvement in cancer treatment with carvacrol and thymol. (dieselok.md)
  • Phase II cytotoxicity (24 h) was due to activation of a "mitochondrially mediated apoptotic program," resulting from PEI-induced channel formation in the outer mitochondrial membrane. (houstonmethodist.org)
  • Using methyl thiazole tetrazolium salt reduction (MTT test), the effects of the AMP-WF3 on cell viability and toxicity were evaluated. (celljournal.org)
  • Although one typically thinks of carbohydrates as associated with cell growth and viability, glycosylation also has an integral role in many processes leading to cell death. (nature.com)
  • Herpes simplex virus thymidine kinase (HSV-tk) is a well-established suicide gene that has been successfully used to control GvHD following donor lymphocyte infusions (DLI) after allogeneic stem cell transplantation. (haematologica.org)
  • Transfer of HSV-tk to DLI preserves the beneficial anti-tumor effect and allows in vivo elimination of donor T cells if severe GvHD occurs. (haematologica.org)
  • 4 In addition, TCRs for minor histocompatibility antigens, including HA-1 and HA-2, have been transferred to donor-derived virus-specific T cells to treat patients with hematologic malignancies after allogeneic stem cell transplantation. (haematologica.org)
  • 2 cGvHD is a multisystemic disorder that occurs due to foreign donor immune cells attacking the recipient's tissues. (haematologica.org)
  • We have defined Phase I toxicity as early necrotic-like changes (30 min) resulting from compromised membrane integrity, assessed by considerable lactate dehydrogenase release and phosphatidylserine translocation from the inner plasma membrane to the outer cell surface. (houstonmethodist.org)
  • Treatment for FL includes successive lines of therapy that unfortunately result in progressively shorter periods of disease-free survival followed by the development of either chemo-refractory disease, large cell transformation or death from treatment-related toxicities ( 2 ). (spandidos-publications.com)
  • This allelic specificity can be overcome by expressing supraphysiologic levels of the wild type MR1 (MR1*01) in cell lines. (bmj.com)