It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. (thermofisher.com)
Far better transfection efficiency than Lipofectamine® 2000 when we compared them head-to-head. (thermofisher.com)
We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
Each year many new products and technologies are launched with improved efficiency and less cytotoxicity. (benthamscience.com)
Choose the general purpose TransFectin Lipid Reagent for high efficiency transfection of your cell lines or primary cells, including difficult-to-transfect cells. (bio-rad.com)
Transfection efficiency and consistency were significantly higher than those following retroviral transduction (87% ± 6 vs. 60% ± 20). (maxcyte.com)
Transfection efficiency ranged from 5.5% with DMRIE Liposomes in rat mesangial cells to 1.1% with LipofectAmine liposomes in human mesangial cells. (mdc-berlin.de)
Effects of PEGylation and Acetylation of PAMAM Dendrimers on DNA Binding, Cytotoxicity and in Vitro Transfection Efficiency. (quantabiodesign.com)
Meanwhile, numerous studies analysed transfection efficiency, vehicle stability and cytotoxicity of distinct chitosan formulations. (gmp-chitosan.com)
They offer new solutions for major issues of gene transfection, improving transfection efficiency for primary cells and transfer rate of large gene sequences. (gmp-chitosan.com)
The transfection of human cell lines by non-viral gene transfections strategies is often restricted by their low transfection efficiency and high toxicity compared to viral-based methods. (gmp-chitosan.com)
The authors of this study analysed whether a 3D chitosan scaffold can improve transfection efficiency for cell lines and primary cells. (gmp-chitosan.com)
The transfection efficiency of the peptides was assessed in HCT 116 colorectal cancer cells and human dermal fibroblasts (HDFs) using high content microscopy. (port.ac.uk)
Both SLPs delivered siRNA and ODNs to HCT 116 colorectal cancer cells with high transfection efficiency which was comparable to the commercial lipid-based transfection reagents, but with higher selectivity for HCT 116 compared to HDFs. (port.ac.uk)
Reagent17
The Effectene Transfection Reagent is intended for molecular biology applications. (qiagen.com)
Due to its low cytotoxicity, Effectene Transfection Reagent is highly suitable for transfection of primary cells and many sensitive cell lines. (qiagen.com)
Transfection using Effectene Transfection Reagent requires low amounts of DNA and minimal handling. (qiagen.com)
In addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. (qiagen.com)
When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable exceptional transfection performance and reproducible results. (thermofisher.com)
Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
BOC Sciences's Hela Transfection Reagent uses unique cationic lipids and is specially used for the efficient delivery of small molecule RNA and DNA in the Hela cell line. (bocsci.com)
Transfection can sometimes seem more like an art than a science-the perfect transfection experiment being dependent on optimization of conditions, including cell density, transfection reagent and DNA:reagent ratio. (promegaconnections.com)
The development of commercially available transfection reagents for gene transfer applications has revolutionized the field of molecular biology and scientific research. (mdpi.com)
Indeed, research shows that transfecting some cell types with any plasmid may cause cell death [ 1 ], while some transfection reagents (e.g., lipids) used for plasmid transfection are themselves toxic to cells [ 2 ]. (idtdna.com)
Synvolux is an independent company based in Leiden, the Netherlands, that develops, manufactures, and markets highly efficient transfection reagents. (synvoluxproducts.com)
Getting DNA or RNA into cells can be a tricky business, and a variety of transfection reagents have been developed over the years to make the process easier. (promegaconnections.com)
Lipid-based reagents are especially popular because they combine efficient transfection with relatively low toxicity. (promegaconnections.com)
Some cell types cannot tolerate plasmid transfection, so one important consideration is direct cytotoxicity due to plasmid transfection itself. (idtdna.com)
Importantly, it has also been shown that plasmid transfections, specifically in CRISPR experiments, can cause cytotoxicity in embryonic stem cells [ 3 ], which could be of concern to many researchers using this cell type. (idtdna.com)
Even with cells that can tolerate plasmid transfections, there are other problems associated with the use of plasmids for CRISPR editing, such as complications in the timing of CRISPR experiments. (idtdna.com)
The neoplastic potential of transformed cells was determined via soft agar cloning and gene transfection analysis. (cdc.gov)
To establish a liposome-based system for transfection of mesangial cells we analyzed the efficacy and toxicity of different cationic liposomes and other nonviral transfection methods in primary cultures of rat and human mesangial cells using the Escherichia coli {beta}-galactosidase (lacZ) gene as a marker. (mdc-berlin.de)
We have previously reported a simple procedure for gene transfection mediated by cationic lipid vesicles for animal cells, in which a commercially available synthetic cationic surfactant, dimethyidioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. (elsevierpure.com)
Spontaneous gene transfection of human bone cells using 3D mineralized alginate-chitosan macrocapsules. (gmp-chitosan.com)
Conclusion: Macrocapsules generated from mineralized polysaccharides display excellent vehicle properties for gene delivery, while chitosan-free or unmineralized capsules were ineffective for transfection. (gmp-chitosan.com)
The 3D scaffold clearly improved the reliability and reproducibility of gene transfection, especially for primary cells, as their transfection is often challenging. (gmp-chitosan.com)
Cellularly, ACD NP exhibited high transfection but low cytotoxicity in HeLa cervical carcinoma cells. (bvsalud.org)
MRNA2
Examples include introducing precise genomic modifications into primary T cells and hematopoietic stem cells via transfection of mRNA or RNPs encoding zinc finger nucleases or CRISPR-Cas9 components. (maxcyte.com)
mRNA transfection is a novel solution for difficult-to-transfect cells such as neurons, primary cells and suspension cells. (bocsci.com)
Vitro2
pEG-containing complexes show increased transfection activity against cells in vitro. (ox.ac.uk)
Purpose To evaluate in vitro cytotoxicity/biocompatibility as well as in vivo tolerability of the novel polysaccharide 4DryField® PH, certified for haemostasis and adhesion prevention. (uni-hannover.de)
Efficient2
Meanwhile, however, many discoveries have been made about the best approaches to CRISPR genome editing-and it turns out that CRISPR plasmids are often not the most efficient approach to use, due to undesired effects such as cytotoxicity and unpredictability, both of which are discussed below. (idtdna.com)
Laser based transfection methods have proven to be an efficient and gentle alternative to established molecule delivery methods like lipofection or electroporation. (uni-hannover.de)
Efficiencies1
Relative colony forming efficiencies (RCE) were used to determine glass fiber cytotoxicity. (cdc.gov)
Methods2
In the following chapter, we will discuss the advantages and disadvantages of different methods of transfection. (benthamscience.com)
Do you want to do transient (short-term) transfections, or are you going to establish a stable cell line? (promegaconnections.com)
Complexes1
Surface charges are slightly shielded by the presence of the hydrophilic polymer, and complexes generally show decreased cytotoxicity compared with simple pLL/DNA complexes. (ox.ac.uk)
Peptides2
The cytotoxicity of the peptides was assessed by standard MTT test. (port.ac.uk)
Moreover, both peptides exhibited very low cytotoxicity even at high concentrations and long exposure time. (port.ac.uk)
Plasmids1
CRISPR plasmids expressing Cas enzymes and guide RNAs have been used for several years, but these plasmids have several limitations, including cytotoxicity and unpredictability of expression. (idtdna.com)
Lipofectamine1
The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
Protein1
Apoptin is a viral protein with well-documented cancer-selective cytotoxicity. (mun.ca)
Cellular1
We further evaluated a subset of samples with strong binding responses for neutralizing, antibody-dependent cellular phagocytosis (ADCP), and antibody-dependent cellular cytotoxicity (ADCC) responses. (cdc.gov)
Molecular1
However, the host intracellular signaling pathways and the molecular mechanisms associated with Acanthamoeba -mediated HBMEC cytotoxicity have not been determined. (asm.org)
Particle1
Possible applications for materials delivery to cells was explored including the cell-particle interface, spiky particle cytotoxicity, and spiky particle-mediated DNAtransfection. (confex.com)
The proprietary mixture of cationic compound and the co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) is designed to provide high expression levels with reduced cytotoxicity. (bio-rad.com)
Figure 2: Development of a Non-Viral, Clinically Adaptable Method to Enhance NK Cell Cytotoxicity Against B Cell Malignancies via CAR Expression. (maxcyte.com)
Transfection with the human renin expression vector led to the secretion of 11 pg/104 cells/48 h human renin in rat mesangial cells, 3600 pg/104 cells/48 h in 293 cells, and 113 pg/104 cells/48 h human renin in opossum kidney cells. (mdc-berlin.de)
Dependent2
Moreover, we showed that Acanthamoeba produces severe HBMEC cytotoxicity by secreting extracellular proteases, as well as using contact-dependent mechanisms such as phagocytosis ( 12 ), which may play an important role in blood-brain barrier perturbations. (asm.org)
Furthermore, it displays no cytotoxicity, which makes it superior to virus-based transfection strategies. (gmp-chitosan.com)
Human1
Human osteosarcoma cell lines Saos-2, MG63 and primary human bone marrow stromal cells (hBMSCs) were chosen as transfection cell model. (gmp-chitosan.com)
System1
The 3D capsule-based transfection system was composed of alginate beads, surrounded by an outer shell of chitosan and calcium phosphate. (gmp-chitosan.com)
Technology1
The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. (qiagen.com)