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  • dendritic
  • Despite deficits in cellular immunity in patients with GBM, CMV pp65-specific T cells could be reliably activated and expanded in vitro using CMV pp65 RNA-pulsed autologous dendritic cells. (aacrjournals.org)
  • The primary goal of this Phase I study was to assess the safety and bioactivity of tumor lysate-pulsed dendritic cell (DC) vaccination to treat patients with glioblastoma multiforme and anaplastic astrocytoma. (aacrjournals.org)
  • This article is from Frontiers in Immunology, volume 5.AbstractOur newly generated murine tumor dendritic cell MuTuDC lines, generated from tumors developing in transgenic mice expressing the simian virus 40 large T antigen SV40LgT and GFP under the DC specific promoter CD11c, reproduce the phenotypic and functional properties of splenic wild type CD8α+ conventional DCs. (duhnnae.com)
  • Approaches used to target this tumor-specific epitope include unarmed and radiolabeled antibody therapy and cell mediated approaches using peptide vaccines and dendritic cells. (wikipedia.org)
  • vivo
  • https://doi.org/10.1084/jem.20171576 ) show that removal of cholesterol from CD8 T cells during type 9 differentiation increases their IL-9 production, persistence in vivo, and cytolytic function against tumors by preventing SUMOylation of liver X receptors. (rupress.org)
  • in this issue) report that Tc9 cells differentiated with minimal cholesterol increases their persistence and increases their antitumor activity in vivo. (rupress.org)
  • However, such protocols are complicated by extensive ex vivo manipulations of tumor-reactive cells and have largely focused on CD8 + CTLs, with much less emphasis on the role and contribution of CD4 + T cells. (rupress.org)
  • We have previously shown that bioactive IL-15 in vivo comprises a complex of the IL-15 chain with the soluble or cell-associated IL-15 receptor alpha chain that are together termed heterodimeric IL-15 (hetIL-15). (jimmunol.org)
  • The expression of WNT16B in the tumor microenvironment attenuates the effects of cytotoxic chemotherapy in vivo, promoting tumor cell survival and disease progression. (wikipedia.org)
  • cytokine
  • The cytolytic and cytokine functions of NK cells are regulated by a complex balance between inhibitory and activating signals that are triggered via the engagement of different NK cell surface receptors with their specific ligands ( 1 , 2 , 3 ). (jimmunol.org)
  • first used Ingenuity Pathway Analysis comparing microarrays from murine cytotoxic CD8 T cells differentiated under type 1 (IL-2 and IL-12) and type 9 (TGF-β and IL-4) cytokine conditions. (rupress.org)
  • Interleukin-15 (IL-15) is a common γ-chain cytokine that plays a significant role in the activation and proliferation of T and NK cells and holds great potential in fighting cancer. (jimmunol.org)
  • HeLa
  • Methanolic and aqueous extracts from 37 seaweed species (10 green and 27 brown seaweeds) collected from Jeju Island coast were prepared at high (70℃) and room (20℃) temperatures and examined for cytotoxic activity against 4 tumor cell lines: U937 (human monoblastoid leukemia cell line), HL60 (human promyelocytic leukemia cell line), HeLa (woman cervical carcinoma cell line) and CT26 (mouse colon carcinoma line). (dbpia.co.kr)
  • one has moderate activity against HeLa tumour cells while the other is inactive. (wikipedia.org)
  • CTLs
  • We set out to investigate one possible explanation for the failure of CTLs to eliminate tumors, specifically, the concept that this failure is not dependent on inhibition of T cell function. (nih.gov)
  • Our data therefore suggest what we believe to be a novel mechanism of MDSC-mediated tumor cell resistance to CTLs. (nih.gov)
  • A ) NO donor (1 hour pre-treatment with 1 mM SIN-1) inhibited killing of EL-4 cells subsequently washed and loaded with specific peptides (SP) by CTLs in chromium release assay. (nih.gov)
  • B ) Pre-treatment of EL-4 cells with 0.1 mM PNT for 10 minutes inhibited killing of target cells by CTLs. (nih.gov)
  • C ) Killing of EL-4 cells that were labeled with 2 doses of CFSE (high and low) and pre-treated with 0.1 mM PNT by CTLs. (nih.gov)
  • melanoma
  • Using a mouse model of advanced melanoma, we found that transfer of small numbers of naive tumor-reactive CD4 + T cells into lymphopenic recipients induces substantial T cell expansion, differentiation, and regression of large established tumors without the need for in vitro manipulation. (rupress.org)
  • Furthermore, blockade of the coinhibitory receptor CTL-associated antigen 4 (CTLA-4) on the transferred CD4 + T cells resulted in greater expansion of effector T cells, diminished accumulation of tumor-reactive regulatory T cells, and superior antitumor activity capable of inducing regression of spontaneous mouse melanoma. (rupress.org)
  • Repeated injections of hetIL-15 were effective in delaying tumor growth in the MC38 colon carcinoma and the B16 melanoma models. (jimmunol.org)
  • induction
  • They associate with different ITAM-transducing polypeptides, including CD3ζ and FcRγ (for NKp30 and NKp46) and KARAP/DAP12 (for NKp44), which permit delivery of intracellular signals resulting in induction of cytotoxic activity ( 9 , 15 , 16 ). (jimmunol.org)
  • 2000. Induction of apoptosis by hydrolysable tannins from Eugenia jambos L. on human leukemia cells. (dbpia.co.kr)
  • To investigate the immune response toward the large T antigen that leads to rejection of the MuTuDC lines, they were genetically engineered by lentiviral transduction to express luciferase and tested for the induction of DC tumors after adoptive transfer in various gene deficient recipient mice. (duhnnae.com)
  • brain tumor
  • This laboratory integrates well with and works closely with The Brain Tumor Center at Duke. (wikipedia.org)
  • Worse outcomes for patients undergoing brain tumor and cerebrovascular procedures following the ACGME resident duty-hour restrictions. (wikipedia.org)
  • peripheral blood mononuclear cells
  • Flow cytometry analysis showed that the bispecific F(ab')2 molecules can bind specifically to cells overexpressing p185HER2 and to normal human peripheral blood mononuclear cells bearing the CD3 surface marker. (rupress.org)
  • Peripheral blood mononuclear cells were differentiated into phenotypically and functionally confirmed DCs. (aacrjournals.org)
  • proliferation
  • Tumor-resident CD8 + T cells showed features of effectors cells and were characterized by increased proliferation (Ki67 + ) and high cytotoxic potential (Granzyme B + ). In the absence of hetIL-15, the smaller population of tumor-infiltrating T cells exhibited high levels of the exhaustion marker PD-1, potentially limiting their anti-cancer effectiveness. (jimmunol.org)
  • Menerba does not activate the ERα-regulated proliferative genes, c-myc and cyclin D1, or stimulate MCF-7 breast cancer cell proliferation or tumor formation, demonstrating that it may be a viable alternative for hormone therapy in comparison to estrogens that non-selectively activate both ER subtypes. (wikipedia.org)
  • It binds, fuses into and replicates within the infected cells' cytoplasm independent of cell proliferation. (wikipedia.org)
  • Cancer
  • Clinical relevance for these data was suggested by the observation that myeloid cells were the predominant source of PNT in human lung, pancreatic, and breast cancer samples. (nih.gov)
  • Here we show that elimination of cancer cells results from a cooperative process executed by multiple CTL engaging sequentially with the same target cell. (aacrjournals.org)
  • The cell lines used in these studies included murine L-1210 leukemia, human RPMI 8226 myeloma, MCF-7 breast cancer and WiDr colon carcinoma. (semanticscholar.org)
  • Radiation survival parameters of antineoplastic drug-sensitive and -resistant human ovarian cancer cell lines and their modification by buthionine sulfoximine. (semanticscholar.org)
  • It has been shown that the increased risk of breast and uterine cancers is associated with ERα activation and that ERβ blocks the growth promoting effects on breast cancer cells. (wikipedia.org)
  • Liquiritigenin did not stimulate uterine size or tumorigenesis of MCF-7 breast cancer cells. (wikipedia.org)
  • PTK7 is expressed by many tumors, including ovarian cancer, breast cancer, and non-small cell lung cancer. (wikipedia.org)
  • Early clinical trial results show a 50% response rate in patients with triple negative breast cancer and an almost complete response in ovarian tumors resistant to platinum-based chemotherapies. (wikipedia.org)
  • LAK cells, along with the administration of IL-2 have been experimentally used to treat cancer in mice and humans, but there is very high toxicity with this treatment - Severe fluid retention was the major side effect of therapy, although all side effects resolved after interleukin-2 administration was stopped. (wikipedia.org)
  • Abnormal MET activation in cancer correlates with poor prognosis, where aberrantly active MET triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). (wikipedia.org)
  • However, cancer stem cells are thought to hijack the ability of normal stem cells to express MET, and thus become the cause of cancer persistence and spread to other sites in the body. (wikipedia.org)
  • In 2011, Memorial Sloan-Kettering Cancer Center researchers found that NDV modified with the viral protein NS1 had a higher specificity for cancer cells that overexpressed the antiapoptotic factor Bcl-xL. (wikipedia.org)
  • Many cancer cells will overexpress antiapoptotic factors as part of tumor development. (wikipedia.org)
  • This mechanism of delaying apoptosis in abnormal cells gives NDV the specificity it needs to be an efficient cancer fighting oncolytic virus. (wikipedia.org)
  • Kuruppu & Tanabe 2005) The precise way in which the presence of NDV induces tumor cell death remains to be clarified and may show variation regarding the strains of NDV used and which type of cancer is targeted. (wikipedia.org)
  • NDV triggers apoptosis in a wide range of cancer cell types via the mitochondrial/intrinsic pathway, through loss of membrane potential and thereby inducing release of cytochrome c in the tumor cell. (wikipedia.org)
  • This gene is predominantly expressed in prostate tissue, and is found to be upregulated in multiple cancer cell lines. (wikipedia.org)
  • It has been proposed that stimulation of WNT16 expression in nearby normal cells is responsible for the development of chemotherapy-resistance in cancer cells. (wikipedia.org)
  • Interleukin
  • A study demonstrated the engineered Hitcher B1 NDV/F3aa strain could be modified to express a highly fusogenic F-protein in combination with immunostimulatory molecules such as IFN-gamma, interleukin 2 or tumor necrosis factor alpha. (wikipedia.org)
  • inhibit
  • These compounds inhibit cell mitosis by binding to the protein tubulin in the mitotic spindle and preventing polymerization or depolymerization into the microtubules. (wikipedia.org)
  • microenvironment
  • This implies a mechanism by which cycles of genotoxic therapy might enhance subsequent treatment resistance in the tumor microenvironment. (wikipedia.org)
  • reactive
  • Similar strategies could greatly affect ACT, perhaps circumventing the problems associated with priming, expansion, and differentiation of tumor-reactive CD4 + T cells in vitro. (rupress.org)
  • syngeneic
  • LAK cells are also capable of acting against cells that do not display the major histocompatibility complex, as has been shown by the ability to cause lysis in non-immunogenic, allogeneic and syngeneic tumors. (wikipedia.org)
  • inhibition
  • Glutathione export by human lymphoid cells: depletion of glutathione by inhibition of its synthesis decreases export and increases sensitivity to irradiation. (semanticscholar.org)
  • Dimethylarsinic acid, DMA(V), caused DNA single stand breaks resulting from inhibition of repair enzymes at levels of 5 to 100 mM in human epithelial type II cells. (wikipedia.org)
  • transgenic
  • Tc9 cells from Apoe −/− mice, a transgenic, atherosclerosis-prone mouse with high levels of cellular and plasma cholesterol, had impaired IL-9 expression and production compared with Tc9 cells from wild-type mice. (rupress.org)
  • human
  • The HER2 protooncogene encodes a 185-kD transmembrane phosphoglycoproteins, human epidermal growth factor receptor 2 (p185HER2), whose amplified expression on the cell surface can lead to malignant transformation. (rupress.org)
  • Human triggering receptors responsible for NK cell activation have been recently identified ( 9 , 10 ). (jimmunol.org)
  • Importantly, this phenotype was recapitulated in human cells. (rupress.org)
  • Interest in the use of NDV as an anticancer agent has arisen from the ability of NDV to selectively kill human tumour cells with limited toxicity to normal cells. (wikipedia.org)
  • molecules
  • Target cells expressing MHC class I (MHC-I) 3 molecules are protected from NK cell lysis by interactions between these molecules and inhibitory NK receptors. (jimmunol.org)
  • synthesis
  • They found that Tc9 cells have lower basal expression of cholesterol synthesis genes, such as Hmgcr and Sqle , and high expression of cholesterol efflux genes, such as Abca1 and Abcg1 , than Tc1 cells. (rupress.org)
  • receptor
  • His current research activities involve the immunotherapeutic targeting of a tumor-specific mutation in the epidermal growth factor receptor. (wikipedia.org)
  • lines
  • The generation of such lines is particularly difficult in recurrent patients, whose tumors have been exposed to radiation. (aacrjournals.org)
  • immune
  • The need for additive "hits" has implications for topographic mechanisms of immune evasion of tumor cells as well as immune intervention to enhance CTL accumulation and cooperation. (aacrjournals.org)
  • The present study indicates that the MHC-I-deficient lung adenocarcinoma had developed mechanisms of escape from the innate immune response based on down-regulation of NCR and ligands required for target cell recognition. (jimmunol.org)
  • These therapies allow a person's own immune cells, often T cells, to specifically target tumor cells while limiting normal tissue damage. (rupress.org)
  • Furthermore, CD169 mediates vesicle trafficking and suppresses the immune response to exosomes-a type of extracellular vesicle released from living cells. (semanticscholar.org)
  • Promising results were discovered with proteins associated to the adaptive immune system, which paved the way for possibilities to use NDV to create a tumor-associated antigen. (wikipedia.org)
  • cytolytic
  • Left: Differentiation of Tc9 cells with low cholesterol decreases the SUMOylation of LXRs, leading to increased production of the cytolytic proteins IFN-γ and Gzmb, increased IL-9 production, possibly increased IL-9R expression, and increased expression of ST2. (rupress.org)
  • lysis
  • In contrast, in the absence of these interactions, target cells become sensitive to NK lysis by triggering of NK cell-activating receptors. (jimmunol.org)
  • cellular
  • Microtubules are the key components of the cytoskeleton of eukaryotic cells and has an important role in various cellular functions such as intracellular migration and transport, cell shape maintenance, polarity, cell signaling and mitosis. (wikipedia.org)
  • myeloid
  • In a previous study, we found that in mice, myeloid-derived suppressor cells (MDSCs) are a source of the free radical peroxynitrite (PNT). (nih.gov)
  • Cladocalol, a formylated triterpene, was isolated from leaves of E. cladocalyx and showed cytotoxic effect on the myeloid leukemia cell line HL-60 [ 5 ]. (hindawi.com)
  • Vesicles
  • The outer surface of the tentacles bears numerous vesicular cells, and there are a few scattered vesicles on the body surface also. (wikipedia.org)
  • proteins
  • The microtubule grows from discrete assembly sites in the cells called Microtubule organizing centers (MTOCs), which are a network of microtubule associated proteins (MAP). (wikipedia.org)
  • These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. (wikipedia.org)
  • Microtubule
  • The addition of tubulin ligands can affect microtubule stability and function, including mitosis, cell motion and intracellular organelle transport. (wikipedia.org)