• The primary aim of the present study was to determine the immunological phenotype of NSCLC-associated macrophages. (ersjournals.com)
  • CD68 + macrophages expressing markers of a cytotoxic M1 phenotype or a noncytotoxic M2 phenotype were identified in the islets and stroma of surgically resected tumours from 20 patients with extended survival (median 92.7 months) and 20 with poor survival (median 7.7 months), using immunohistochemistry. (ersjournals.com)
  • Macrophages infiltrating the tumour islets in nonsmall cell lung cancer were predominantly of the M1 phenotype in patients with extended survival. (ersjournals.com)
  • The M1 phenotype (classically activated) macrophages are thought to be induced by interferon-γ, with or without lipopolysaccharide and tumour necrosis factor (TNF)-α, and exert a cytotoxic effect against cancer cells. (ersjournals.com)
  • Additionally, M2 macrophages have been traditionally thought of as being the predominant macrophage phenotype in solid tumours (tumour-associated macrophages). (ersjournals.com)
  • Recent evidence, however, supports a dual role for macrophages in the regulation of tumour proliferation and immune control 10 , 16 , and indicates that the local tissue microenvironment plays a critical role in determining cell phenotypes. (ersjournals.com)
  • Kupffer cells, hepatic stellate cells and sinusoidal endothelium) and infiltrating leukocytes (e.g., monocytes, monocyte-derived macrophages, neutrophils and lymphocytes). (mdpi.com)
  • The leukocyte donor to recipient ratio varied with rejection status for macrophages and with time post-transplant for lymphocytes. (biorxiv.org)
  • In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
  • However, due to the release of proinflammatory cytokines and cytotoxic radical species, uncontrolled activity of macrophages may also be detrimental to tissue repair. (hindawi.com)
  • In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
  • Classically, activated M1 macrophages release pro-inflammatory cytokines and activate type1 T cell response that has a cytotoxic effect on tumor cells, whereas M2 macrophages produce proteolytic enzymes, suppress immune response, and contribute to hypoxia-induced angiogenesis, thus promoting tumor cell proliferation and migration [ 5 ]. (springeropen.com)
  • In strong tumors, bidirectional interaction between macrophages as well as the tumor interstitium shapes their phenotype. (translateinthetownships.com)
  • In contrast to macrophages, tumor-infiltrating cytotoxic T lymphocytes (TILs), like CD8+ T cells, are generally related with good prognosis (21). (translateinthetownships.com)
  • however, due to constitutive mTORC1 activation, these cells retained a terminally differentiated effector phenotype and were incapable of transitioning into a memory state. (jci.org)
  • If the collaborative requirement of Lyt 1 T helper cells is bypassed by the Lyt 1 T cell-derived mediator of T help, termed I1-2, upon antigenic stimulation, PNA + Lyt 123 thymocytes differentiate into either alloreactive or H-2-restricted PNA - Lyt 23 cytotoxic effector cells. (psu.edu)
  • Along the differentiation pathway from Lyt 123 → 23 effector cells, cytolytic activity is carried out by T cells that still express the Lyt 123 phenotype. (psu.edu)
  • Recipient origin T cells expressed cytotoxic and pro-inflammatory genes consistent with an effector cell phenotype whereas donor origin T cells are likely quiescent expressing oxidative phosphorylation genes relative to recipient T cells. (biorxiv.org)
  • The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). (neobiotechnologies.com)
  • The NK-1 monoclonal antibody specifically reacts with a 110 kDa carbohydrate antigen associated with myelin-associated glycoprotein expressed on 7-35% of normal peripheral blood lymphocytes including a subset of natural killer cells, a subset of CD8-positive peripheral blood T cells, and on some neural tissues. (bdbiosciences.com)
  • Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. (edu.au)
  • To determine the immunologic characteristics of tumor infiltrating lymphocytes (TILs) in comparison with the corresponding peripheral blood lymphocytes (PBLs) of patients with ovarian cancer in order to detect specific antitumor-reactive-immunocompetent cells. (nih.gov)
  • Peripheral blood lymphocytes had a higher proliferative activity and cytotoxicity against natural killer-sensitive tumor cells than the corresponding TILs. (nih.gov)
  • Peripheral blood lymphocytes showed more potentially valuable behavioral characteristics than TILs. (nih.gov)
  • In this mesenchymal subtype, a decreased number of CD8 + tumor-infiltrating lymphocytes (TILs) were detected, suggesting an association between EMT tumor cells and exclusion of these immune cells from the TME. (encyclopedia.pub)
  • Therefore, in the last years characterization of macrophage phenotype in vivo during tissue repair has been a matter of active investigation. (hindawi.com)
  • Abnormal movement of lytic granules and reduced perforin content in cytotoxic T lymphocytes and natural killer cells define their respective defects in cytolytic activity. (medscape.com)
  • We previously reported that CD8 + T cells in human gastrointestinal mucosa exhibit reduced perforin expression and weak or impaired cytotoxic capacity compared with their counterparts in blood. (aai.org)
  • Rectosigmoid CD8 + T cells appeared skewed toward cytokine production rather than cytotoxic responses, with cells expressing multiple cytokines and chemokines generally lacking in perforin and Gzm expression. (aai.org)
  • Besides their role as cytotoxic cells through the production of perforin and granzymes, NK cells are potent producers of cytokines like Interferon (IFN)-γ and Tumor Necrosis Factor (TNF)-α [ 3 ] and thus play important roles in immunomodulation and the defence against viral, parasitic and bacterial pathogens [ 4 ]. (biomedcentral.com)
  • Genotype-phenotype study of familial haemophagocytic lymphohistiocytosis due to perforin mutations. (lu.se)
  • The essential role of FAS plays in maintaining lymphocyte homeostasis and peripheral immune tolerance to prevent autoimmunity was first demonstrated by studying FAS-deficient MRL/lpr-/- mice. (medscape.com)
  • Flow cytometric analysis of CD57 expression on human peripheral blood lymphocytes. (bdbiosciences.com)
  • In this situation, peripheral activated T lymphocytes can pass through the blood-retinal barrier because of the T cell receptors and similar polypeptides in the retina, resulting in endophthalmitis[7],[16]. (mingsheng88.org)
  • However, at least two distinct macrophage populations have been identified: the classically activated M1 phenotype and the alternative activated M2 phenotype [ 6 ]. (hindawi.com)
  • Carcinoma cells that undergo an epithelial-mesenchymal transition (EMT) and display a predominantly mesenchymal phenotype (hereafter EMT tumor cells) are associated with immune exclusion and immune deviation in the tumor microenvironment (TME). (encyclopedia.pub)
  • An association between EMT tumor cells (mesenchymal and hybrid epithelial-mesenchymal phenotypes) and increased numbers of infiltrating PD-1 + cells was also observed in another study in patients with adenocarcinoma of the lung [ 6 ] . (encyclopedia.pub)
  • Weinberg characterized "aberrant metabolic phenotype" with "autologous proliferation signaling, apoptosis resistance, evasion of proliferation inhibition, continuous angiogenesis, infiltration and migration, unlimited replication capacity, immune escape" in tumor cells. (biomedcentral.com)
  • However, rather than actively modifying the environment, multiple mechanisms guide the evolution of tumor cells through the selection of subclones with the most adaptive phenotype by environmental factors ( 1 ). (spandidos-publications.com)
  • Tumor-specific neoantigens, which are expressed on tumor cells, can induce an effective antitumor cytotoxic T-cell response and mediate tumor regression. (wjgnet.com)
  • Specifically, we observed increased numbers of BAL central memory T cells, evidence of type I polarization, and decreased expression of cytotoxic T lymphocyte-associated protein 4 and programmed cell death protein 1 in BAL Tregs, suggesting both activation of proinflammatory subsets and an attenuated suppressive phenotype. (jci.org)
  • In the current study it is shown that this NKp46 high phenotype was correlated with an increased expression of CD16 and CD27 compared to the CD8α + NKp46 - and NKp46 + NK-cell subsets in spleen and blood. (biomedcentral.com)
  • Nevertheless, a recent study from our group with newly developed monoclonal antibodies (mAbs) against the activating receptor NKp46 enabled a more comprehensive insight into the phenotype of porcine NK cells and putative subsets [ 20 ]. (biomedcentral.com)
  • CD4+ T-cells and other lymphocyte subsets in persons infected with human immunodeficiency virus (HIV). (cdc.gov)
  • The guidelines describe single-platform technology (SPT), a process in which absolute counts of lymphocyte subsets are measured from a single tube by a single instrument. (cdc.gov)
  • With CD45 gating, the relative numbers of beads and lymphocyte subsets are enumerated, and their absolute numbers and percentage values are calculated. (cdc.gov)
  • 4 According to the number of tumor-infiltrating lymphocytes, tumors can be divided into three phenotypes: immune-inflamed, immune-excluded, and immune-desert types. (nature.com)
  • [ 2 ] , is caused by a failure of apoptotic mechanisms to maintain lymphocyte homeostasis leading to abnormal lymphocyte survival. (medscape.com)
  • The ceramide-induced down-regulation of PKC results in abnormal cellular phenotypes, suggesting these metabolic cascades are responsible for the beige phenotype [ 13 ]. (biomedcentral.com)
  • 6 months) non-malignancy and non-infectious uncontrolled proliferation of lymphocytes commonly accompanied by autoimmune manifestations, lymphadenopathy, splenomegaly, and susceptibility to malignancies. (medscape.com)
  • Tumor infiltrating lymphocytes and PBLs were phenotyped by their surface markers, cytokine pattern, proliferation rate, and cytotoxic ability. (nih.gov)
  • T cell immunity is at the center of the disease contributing to the inflammatory process through the loss of tolerance to gluten and the differentiation of HLA-DQ2 or HLA-DQ8-restricted anti-gluten inflammatory CD4 + T cells secreting pro-inflammatory cytokines and to the killing of intestinal epithelial cells by cytotoxic intraepithelial CD8 + lymphocytes. (frontiersin.org)
  • However, some immune cells are immunosuppressive or transform into immunosuppressive phenotypes under specific conditions, leading to the development of radioresistance. (nature.com)
  • Because the expression of the beta-3A subunit is normally ubiquitous, deficiency of the beta-3A subunit leads to a precise phenotype in cells with a large number of intracellular granules (eg, neutrophils, natural killer cells, cytotoxic T lymphocytes, platelets, melanocytes). (medscape.com)
  • Consistent with downregulated MHC I expression, the elimination of Nlrc5(Δ/Δ) lymphocytes by cytotoxic T cells was markedly reduced and, in addition, we observed low NLRC5 expression in several murine and human lymphoid-derived tumor cell lines. (unige.ch)
  • Human influenza A virus-specific, cytotoxic T cells have been shown previously to recognize the virus nucleoprotein on infected cells. (ox.ac.uk)
  • CD8+ CTL lines were derived from lymphocytes of two HLA B37-positive donors and used to show that the peptide was represented on virus-infected cells and to determine the probable boundaries of the epitope. (ox.ac.uk)
  • Immunized T-lymphocytes which can directly destroy appropriate target cells. (edu.au)
  • Phenotypical analyses with newly developed monoclonal antibodies (mAbs) against porcine NKp46 recently revealed that in blood NKp46 - and NKp46 + cells with NK phenotype exist with comparable cytotoxic properties. (biomedcentral.com)
  • Additionally, a third NK cell population with elevated NKp46 expression levels was identified in high frequencies in spleen and liver, pointing towards a special role of NK cells with this phenotype. (biomedcentral.com)
  • The development of skin sensitization is associated with, and requires, the activation and clonal expansion of allergen responsive T lymphocytes and it is these cells that orchestrate the cutaneous allergic reaction. (cdc.gov)
  • Intracellular antigens (eg, viruses) can be processed and presented to CD8 cytotoxic T cells by any nucleated cell because all nucleated cells express class I MHC molecules. (msdmanuals.com)
  • Macrophage activation has been described as a dynamic process: the same cell may initially induce proinflammatory and cytotoxic reactions and later may take part in the resolution of inflammation and wound healing [ 9 ]. (hindawi.com)
  • The phenotypes of both lymphocyte populations were very heterogeneous. (nih.gov)
  • The task of the researcher is to define a unifying set of parameters to study (ie carefully define the phenotype), and to seek out and interpret the informative mating experiments that are hidden within modern populations. (biomedcentral.com)
  • Our data show that NLRC5 acts as a key transcriptional regulator of MHC I in lymphocytes and support an essential role for NLRs in directing not only innate but also adaptive immune responses. (unige.ch)
  • Maturing DCs acquire a phenotype that allows them to migrate through the lymphatic system and stimulate adaptive lymphocyte responses. (jscimedcentral.com)
  • The survival advantage conferred by islet macrophage infiltration may be related to their cytotoxic antitumour activity. (ersjournals.com)
  • CeD is characterized by an infiltration of intraepithelial lymphocytes in the proximal part of the small intestine, crypt hyperplasia and the development of villous atrophy in the latest stages of the disease. (frontiersin.org)
  • Identification of novel therapeutic targets, stratified by histological as well as mutational and transcriptional phenotypes, should transform survival prospects. (lu.se)
  • In addition, this would also preclude those that exhibit clinical phenotypes of adolescent and adult CHS from this treatment. (biomedcentral.com)
  • The difficulty of precisely defining the clinical phenotype, as well as underlying genetic heterogeneity, complicates the problem. (biomedcentral.com)
  • Genetic subtypes of familial hemophagocytic lymphohistiocytosis: correlations with clinical features and cytotoxic T lymphocyte/natural killer cell functions. (lu.se)
  • In a previous study, the utility of CD4+ T lymphocytes in autoactivation was associated with the pathogenesis of autoimmune disorders. (mingsheng88.org)
  • The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552). (neobiotechnologies.com)
  • In response to environmental cues they display a proinflammatory (M1) or an alternative anti-inflammatory (M2) phenotype. (hindawi.com)
  • After the loss of the cytotoxic T lymphocyte (CTL) response to this epitope and in the presence of high viral load, reversion to wild-type sequence was observed. (ox.ac.uk)
  • HLA B37 determines an influenza A virus nucleoprotein epitope recognized by cytotoxic T lymphocytes. (ox.ac.uk)
  • These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. (edu.au)
  • Prior to the advent of cytotoxic chemotherapy, lung cancer at all stages was treated surgically or by radiation alone ( 5 , 6 ). (frontiersin.org)
  • Autoimmune lymphoproliferative syndrome (ALPS) is a rare genetic disorder of lymphocyte homeostasis. (medscape.com)
  • Patients with an absence of cytotoxic T lymphocyte (CTL) function have a high risk for developing HLH, and could therefore benefit the most from early hematopoietic stem cell transplantation (HSCT). (biomedcentral.com)
  • In this article we show that these animals exhibit slightly decreased CD8(+) T cell percentages, a phenotype compatible with deregulated MHC I expression. (unige.ch)
  • RESULTS: Patients in groups 1 and 2 had CD4+ T-lymphocyte counts that were similar to those in patients in groups 4 and 5, and they were not infected with the human immunodeficiency virus (HIV). (bvsalud.org)
  • The study incorporates disciplines such as Virology, Phenotype, Gene, Selective IgA deficiency and B-cell activating factor in addition to Common variable immunodeficiency. (research.com)
  • Lavage fluid parameters (lactate dehydrogenase and albumin), cellular influx into the lung (macrophage, neutrophil, and lymphocytes), and LALN cell number and phenotype were evaluated to assess lung injury, inflammation, and immune response. (cdc.gov)
  • the relevance of HIF as a tumor driver offers been recently challenged by the statement that the genetic ablation 607742-69-8 supplier of HIF in Fh1-deficient mice did not abolish cyst formation but, on the in contrast, exacerbated this phenotype [8]. (immune-source.com)
  • In contrast, NLRC5 deficiency dramatically impaired basal expression of MHC I in T, NKT, and NK lymphocytes. (unige.ch)
  • Single-platform technology (SPT) is designed to enable de- system and managing the health care of persons infected with terminations of both absolute and percentage lymphocyte sub- human immunodeficiency virus (HIV) ( 1-4 ). (cdc.gov)
  • The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. (bdbiosciences.com)
  • The role of macrophage phenotypes in tumour progression has been extensively reviewed 6 - 10 . (ersjournals.com)
  • ACAP1 plays a key role in endocytic recycling, which is essential for the normal function of lymphocytes. (mdpi.com)
  • A majority of DCs in circulation and the mucosal periphery,the main sites of early HIV interaction, are present in an immature phenotype [13]. (jscimedcentral.com)
  • In spleen a third NKp46-defined population with NK phenotype was observed that was characterised by a low to negative CD8α and increased NKp46 expression. (biomedcentral.com)
  • Defective cytotoxic lymphocyte degranulation in syntaxin-11 deficient familial hemophagocytic lymphohistiocytosis 4 (FHL4) patients. (lu.se)
  • et 20 témoins en bonne santé ne présentant pas d'infection par le virus de l'hépatite C. Une réduction importante de la fréquence des cellules tueuses naturelles totales dans le groupe des patients porteurs d'une infection chronique a été observée par rapport au groupe des témoins ( P = 0,001) ou au groupe des patients dont l'infection a connu une résolution spontanée ( P = 0,01). (who.int)