• The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including PRKRA, TRBP, and Dicer. (hindawi.com)
  • They are formed by two hemichannels, made up of six connexin proteins assembled in groups. (nih.gov)
  • The objective is to yield supported membranes with a bilayer to substrate spacing that can accommodate membrane proteins with large cytoplasmic domains. (aiche.org)
  • Cloned Kir channel cDNAs encode proteins of between ~370-500 residues, both N- and C-termini are thought to be cytoplasmic, and the N terminus lacks a signal sequence. (embl.de)
  • RNA binding proteins recognize these sequences and assemble with their mRNA target into mRNPs. (europa.eu)
  • to postulate that CLIC1 is among the small group of proteins which are assembled as soluble cytoplasmic proteins, which will then insert themselves into the appropriate membrane via their own mechanism. (proteopedia.org)
  • Nuclear mRNA transcripts acquire a protein coat composed of cap- and RNA-binding proteins that allow nuclear export and dictate the functional program of cytoplasmic mRNAs ( Moore, 2005 ). (rupress.org)
  • This mitochondrial RNA colocalizes with components of the cytoplasmic machinery that makes and imports nuclear-encoded proteins - that is, a ribosomal protein (S6), a nascent peptide associated protein (NAC), and the translocase in the outer membrane (Tom22). (biomedcentral.com)
  • Then, proteins encoded by the nuclear genome and destined for the mitochondria will be made close to mitochondrial-encoded proteins so that they can be assembled efficiently into mitochondrial complexes. (biomedcentral.com)
  • During glucose deprivation, a stress that isn't known to cause unfolding of proteins, I have observed Hsp104, Hsp26, and Hsp42 but not Ssa1 form into cytoplasmic foci. (uth.edu)
  • Viral proteins and genomic RNAs aggregates in cytoplasmic viral factories . (expasy.org)
  • The assembly platform (comprising proteins CEFLM) is at the centre of the secretion system and interacts with the outer membrane secretin D via the periplasmic domains of CLM and with the cytoplasmic ATPase E via the cytoplasmic domain of L. At the heart of the assembly platform is the potential rotor protein F which facilitates the assembly of the short periplasmic pilus comprising the major pilin G and initiation pilins IJK. (ukri.org)
  • We were particularly interested in exploring how protein purification, self-assembling and endosomal escape perform in proteins containing the variant histidine-rich tags. (upc.edu)
  • The numer- ical modelling of cell penetration and endosomal escape of the tested proteins has revealed a negative relationship between the amount of protein internalized into target cells and the efficiency of cytoplasmic release. (upc.edu)
  • SummaryType III intermediate filament (IF) proteins assemble into cytoplasmic homopolymeric and heteropolymeric filaments with other type III and some type IV IFs. (knaw.nl)
  • Context and state of the art mRNA transport in general: Cytoplasmic mRNA transport and local translation are essential for many processes requiring symmetry breaking such as embryonic development, cell migration and neuronal differentiation (1). (europa.eu)
  • Since the first discovery of cytoplasmic mRNA transport almost three decades ago, several essential questions could not be answered mostly due to the complexity of the in vivo situation and the approaches available. (europa.eu)
  • Cytoplasmic IFs do not undergo treadmilling like microtubules and actin fibers, but are dynamic. (wikipedia.org)
  • mRNAs are transported along microtubules (MTs) by different kinesins and cytoplasmic dynein and get anchored at the actin cortex, intermediate filaments or unknown structures (1, 3). (europa.eu)
  • Actin is powered by ATP to assemble its filamentous form, which serves as a track for the movement of a motor protein called myosin. (lumenlearning.com)
  • This enables actin to engage in cellular events requiring motion, such as cell division in animal cells and cytoplasmic streaming, which is the circular movement of the cell cytoplasm in plant cells. (lumenlearning.com)
  • Importantly, cells that had forced nuclear fascin had significantly reduced growth rates and viability because they assembled large stable actin bundles in the nucleus which prevented them from going through the cell cycle. (elifesciences.org)
  • Scar/WAVE-1, a Wiskott-Aldrich syndrome protein, assembles an actin-associated multi-kinase scaffold. (eu.org)
  • Monomeric actin (G-actin) can self assemble (polymerize) into microfilaments (F-actin), the fundamental unit of the actin cytoskeleton. (tebubio.com)
  • Taken together, these data suggest that multiple viral components, including assembled nucleocapsids, have important roles in the paramyxovirus budding process. (nih.gov)
  • Reoviruses replicate and assemble within cytoplasmic inclusions known as viral factories. (biongenex.com)
  • The capsid is assembled on the sub-viral particle. (expasy.org)
  • Respiratory syncytial virus (RSV) RNA synthesis takes place in cytoplasmic viral factories also called inclusion bodies (IBs), which are membrane-less organelles concentrating the viral RNA polymerase complex. (bvsalud.org)
  • FtsZ is a cytoskeletal protein that participates in the formation, on the inner side of the cytoplasmic bacterial membrane, of the "septal ring", a protein complex responsible for cell division [1]. (cea.fr)
  • The compounds allowed for concentration-dependent recovery of GPI biosynthesis and were highly active on the cytoplasmic face of the endoplasmic reticulum membrane. (fhnw.ch)
  • Secretion is achieved by an inner-membrane complex assembling a short pilus to push the recruited effector from the periplasm (the space between the two membranes) through the gated pore in the outer-membrane. (ukri.org)
  • As a result of this work, we will determine the structure of the inner-membrane assembly platform and its interaction with the cytoplasmic ATPase. (ukri.org)
  • We have assembled the reagents and expertise needed to determine the structure of the inner membrane assembly platform of the type II secretion system. (ukri.org)
  • Our results indicated that VirE2 co-localized with VirE3 on the cytoplasmic side of the host cellular membrane upon the delivery. (figshare.com)
  • Glial cytoplasmic inclusions (GCIs) and neuronal multisystem degeneration are the pathologic hallmarks of this clinically variable disorder (see the image below). (medscape.com)
  • Intriguingly, cytoplasmic lattices were disorganized, and mitochondria, endoplasmic reticulum, and components of the subcortical maternal complex were mislocalized. (life-science-alliance.org)
  • RISC assembles into processing bodies (P-bodies) which are specific cytoplasmic foci in somatic eukaryotic cells [ 7 - 9 ]. (hindawi.com)
  • On the other hand, there are specific cytoplasmic foci similar to the P-bodies in spermatogenic cells, which are called nuage [ 15 - 18 ]. (hindawi.com)
  • Cytoplasmic IFs assemble into non-polar unit-length filaments (ULFs). (wikipedia.org)
  • We have used supported lipid bilayers and controlled orientation of FtsZ monomers in vitro to study their GTP-dependent self-assembling on surfaces with atomic force microscopy in solution, providing single molecule information of the dynamic structure of filaments and their aggregates [2-4]. (cea.fr)
  • Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. (nih.gov)
  • Ciliary motility is driven by axonemal dyneins that are assembled in the cytoplasm before deployment to cilia. (wustl.edu)
  • Under this proposal, we will expand upon these successes and demonstrate assembly of two classes of full-length human antibodies from both the chloroplast and nucleo-cytoplasmic compartments. (grantome.com)
  • 2) Assemble a Fab and full-length human antibody in algal chloroplast. (grantome.com)
  • Based on mitotype-specific markers and mitotype-variant ORFs, four main cytoplasmic haplotypes were identified in our groups corresponding the nap , pol , ole , and cam mitotypes, among which the structure of chloroplast genomes was more conserved without any rearrangement than mitochondrial genomes. (biomedcentral.com)
  • Based on whole-genome variants diversity analysis, eleven genetic difference regions among different cytoplasmic haplotypes were identified on chloroplast genomes. (biomedcentral.com)
  • This protein is 1,353 amino acids in length, and the monomers assemble to form a homotrimer. (frontiersin.org)
  • Besides, the organelle pan-genomes and the overall variation data offered useful resources for analysis of cytoplasmic inheritance related agronomical important traits of rapeseed, which can substantially facilitate the cultivation and improvement of rapeseed varieties. (biomedcentral.com)
  • Our nuclear transfer experiment showed that the phenotype is attributable to cytoplasmic rather than nuclear defects of the zygotes. (life-science-alliance.org)
  • It is involved in a large number of cellular processes, including muscle contraction, lamellopodial extrusion, cell locomotion, cytokinesis, intracellular transport and cytoplasmic streaming. (tebubio.com)
  • The defined variable loops enabled us to investigate assembled Env-glycan shields throughout SIV, which we found to comprise both N- and O-linked glycans, the latter emanating from V1 inserts, which bound the O-link-specific lectin jacalin. (yale.edu)
  • Collectively, this study demonstrates that the anchorage protein VirE3 uses the two tandem VirE2-interacting domains to facilitate VirE2 protection for T-DNA at the cytoplasmic side of the host cell entrance. (figshare.com)
  • We designed, synthesized, and used GPI fragments to rescue the biosynthesis of GPI-APs caused by a mutation in genes involved in assembling GPI-glycolipids in cells. (fhnw.ch)
  • Most types are cytoplasmic, but one type, Type V is a nuclear lamin. (wikipedia.org)
  • Furthermore, truncation of the HN cytoplasmic tail was found to be inhibitory toward budding, since it prevented coexpressed wild-type (wt) F protein from directing VLP budding. (nih.gov)
  • Conversely, truncation of the F protein cytoplasmic tail was not inhibitory and did not affect the ability of coexpressed wt HN protein to direct the budding of particles. (nih.gov)
  • Motile ciliopathy can result from defects in the dyneins themselves or from defects in factors required for their cytoplasmic pre-assembly. (wustl.edu)
  • So the team wanted to understand whether moving all fascin into the nucleus would prevent the cytoplasmic function of fascin. (elifesciences.org)
  • The substrates were constructed by initially forming p-aminophenyltrimethoxysilane (APhMS) self-assembled monolayers on silica microspheres followed by subsequent attachment of biotin-PEG3400-NHS, PEG2000-NHS (for added passivation), and finally streptavidin. (aiche.org)
  • These structures first appear in the cytoplasmic lobe of elongated step 10 spermatids and subsequently disappear in step 19 spermatids. (hindawi.com)
  • CsCl density gradient centrifugation indicated that almost all of the NP protein in the cells had assembled into nucleocapsid-like structures. (nih.gov)
  • Cutaneous acanthamoebiasis is a rare diagnosis that carries a mortality rate of over 70%.2 This disease predominantly affects immunocompromised individuals, though infections have been reported in immunocompetent individuals.2 We report a fatal case of cutaneous Acanthamoeba infection in a patient with granulomatous vasculitis on biopsy, initially thought to be antineutrophil cytoplasmic antibody (ANCA)-negative vasculitis. (cdc.gov)
  • PRKRA is contained in the cytoplasmic RNA-induced silencing complex (RISC) together with transactivation response RNA binding protein (TRBP) and Dicer [ 4 - 6 ]. (hindawi.com)
  • However, little is known about how the T-complex is assembled inside host cells. (figshare.com)
  • Cas endodeoxyribonucleases assemble with a guide RNA complex, a hybrid of CRISPR RNA (crRNA) and transactivating crRNA (tracrRNA) molecules, to form an active complex that cleaves DNA. (bvsalud.org)
  • Meltrin alpha cytoplasmic domain interacts with SH3 domains of Src and Grb2 and is phosphorylated by v-Src. (eu.org)
  • Cytoplasmic RNA granules in germ cells (polar and germinal granules), somatic cells (stress granules and processing bodies), and neurons (neuronal granules) have emerged as important players in the posttranscriptional regulation of gene expression. (rupress.org)
  • Some mRNAs are programmed for immediate translation, a process that remodels the protein coat and assembles a polysome. (rupress.org)
  • Thus, maternal UHRF1 regulates the proper cytoplasmic architecture and function of oocytes and preimplantation embryos, likely through a mechanism unrelated to DNA methylation. (life-science-alliance.org)
  • The presence of cortical Lewy bodies is confirmed by the finding of alpha synuclein positive rounded cytoplasmic inclusion in neurons. (medscape.com)
  • The energy needed for pilus assembly is provided by the cytoplasmic ATPase. (ukri.org)
  • Deletion of the F and HN cytoplasmic tails indicated an important role of these cytoplasmic tails in VLP budding. (nih.gov)