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Syndecan-2CytoplasmSyndecan-3ProteoglycansMolecular Sequence DataMembrane GlycoproteinsAmino Acid SequenceRecombinant Fusion ProteinsProtein BindingSequence Homology, Amino AcidProtein Structure, TertiaryCell MembraneSynteninsTransfectionCell LineBase SequenceSignal TransductionMembrane ProteinsRecombinant ProteinsCloning, MolecularBinding SitesMutagenesis, Site-DirectedSequence Deletionsrc Homology DomainsProtein ConformationCell AdhesionHeparan Sulfate ProteoglycansCHO CellsProtein Interaction Domains and MotifsAmino Acid MotifsCricetinaeStructure-Activity RelationshipPhosphorylationTooth GermSequence AlignmentHeparitin SulfateMutationDNA, ComplementaryProtein Structure, SecondaryDimerizationTyrosineKineticsCells, CulturedPeptide FragmentsCell Adhesion MoleculesAntigens, CDModels, MolecularCarrier ProteinsPrecipitin TestsIntegrinsConserved SequenceMutagenesisEndocytosisTwo-Hybrid System TechniquesDNACrystallography, X-RayRNA, MessengerLigandsAnion Exchange Protein 1, ErythrocyteDNA PrimersIntegrin beta3PDZ DomainsAmino Acid SubstitutionCytoskeletal ProteinsNerve Tissue ProteinsProtein TransportDNA-Binding ProteinsEscherichia coliElectrophoresis, Polyacrylamide GelCercopithecus aethiopsAntigens, CD29Point MutationSequence Homology, Nucleic AcidModels, BiologicalTenascinProtein-Tyrosine KinasesFibronectinsAnkyrinsHeLa CellsGene ExpressionImmunoblottingBacterial ProteinsCell Adhesion Molecules, NeuronalDrosophila ProteinsTumor Cells, CulturedPhosphoproteinsPlasmidsTranscription FactorsProtein Sorting SignalsTalinBlotting, WesternReceptors, ImmunologicGolgi ApparatusMicroscopy, FluorescenceMacromolecular SubstancesPeptidesCell MovementGlutathione TransferaseCytoskeletonPolymerase Chain Reaction
ProteinProteinsProteoglycansProteoglycanShort cytoplasmicBindsHeparan sulfate chainsPolyadenylation elementEpithelialCellular uptakeSynteninMammalianIsoformBindExtracellular domainsRyudocanAntibodyNuclearTransmembrane domainsInteractsReceptorRegulationNeuronsCartilageDifferentiationSpeciesMediateMoleculeGeneTailAminoFocalAbhydrolase domainGrowthSequenceSubfamilyCoiled-coil domainCellFamilyMolecularKinaseInteractionPeptidePhysiologicalHighlyFragments
Protein21
  • Syndecan-4 is a protein that in humans is encoded by the SDC4 gene. (wikipedia.org)
  • Their intracellular domain of membrane-spanning core protein interacts with actin cytoskeleton and signaling molecules in the cell cortex. (wikipedia.org)
  • The protein is found as a homodimer and is a member of the syndecan proteoglycan family. (wikipedia.org)
  • Syndecan-4 activates protein kinase C (PKC), an enzyme involved in signal transduction. (wikipedia.org)
  • Regulation at the protein level includes modulation of its cell surface expression, cathepsin L-mediated processing, cellular uptake, secretion, and cytoplasmic vs. nuclear localization. (pafr-signal.com)
  • This function of heparanase appears independent of its enzymatic activity and HS substrate Talazoparib solubility dmso and is mediated by a protein domain localized at the C-terminus (C-domain) of the protein. (pafr-signal.com)
  • To further define which neurons express Eph, antibodies were generated to the cytoplasmic portion of the Eph protein. (sdbonline.org)
  • The syndecans, heparan sulfate proteoglycans, are abundant molecules associated with the cell surface and extracellular matrix and consist of a protein core to which heparan sulfate chains are covalently attached. (embrapa.br)
  • These protein domains locate the syndecan on cell surface sites during development and tumor formation where they interact with other receptors to regulate signaling and cytoskeletal organization. (embrapa.br)
  • The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. (thermofisher.com)
  • The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. (thermofisher.com)
  • Chimeric antigen receptor (CAR) T cell therapy has emerged as a novel immunotherapy which modifies T cells with CAR, an artificial fusion protein that incorporates an extracellular antigen recognition domain, a transmembrane domain, and an intracellular domain including costimulation and signaling components [ 4 , 5 ]. (biomedcentral.com)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing PDZ domain in the selected taxonomic class. (embl.de)
  • In this work, we show that substrate recognition of Tsp is mediated by a PDZ domain, a small protein module that promotes protein-protein interactions by binding to internal or C-terminal sequences of their partner proteins. (embl.de)
  • The isolated PDZ domain (amino acids 206-334) is capable of folding into a well-behaved structure and binds to a nonpolar peptide with a dissociation constant (K(D)) of 1.9 microM, similar to that of the intact Tsp protein. (embl.de)
  • The use of a separate substrate recognition domain such as a PDZ domain may be a general mechanism for achieving selective protein degradation. (embl.de)
  • Dally, an ortholog of the mammalian glypican 3/5 subfamily, and Dally-like protein, an ortholog of the glypican 1/2/4/6 subfamily. (biomedcentral.com)
  • formin binding protein 4 [Source:HGN. (gsea-msigdb.org)
  • Fry protein is composed of an N-terminal Furry domain (FD) with HEAT/Armadillo repeats followed by five regions without any recognizable functional domains. (nature.com)
  • D) Cytoplasmic and nuclear ZKSCAN5 protein levels in two types of breast cancer cell lines, ZR75-1 and MDA-MB-231. (bms-911543.com)
  • Tubulin was used as the cytoplasmic control, and lamin A/C was used as the nuclear protein-loading control. (bms-911543.com)
Proteins15
  • Syndecans interact with fibronectin on the cell surface, cytoskeletal and signaling proteins inside the cell to modulate the function of integrin in cell-matrix adhesion. (wikipedia.org)
  • Matrix molecules, growth factors, and enzymes) Chondroitin sulfate chain Transmembrane domain - self-association C1 domain - actin-association cytoskeleton Variable domain - syndecan-specific C2 domain - attach to PDZ proteins Syndecans normally form homodimers or multimers. (wikipedia.org)
  • Syndecans interact with the intracellular cytoskeleton through the cytoplasmic domains of their core proteins and membrane proteins, extracellular enzymes, growth factors, and matrix components, through their heparan-sulfate chains, to regulate developmental processes. (tokushima-u.ac.jp)
  • Here, as a first step to assess the possible roles of syndecan proteins in amelogenesis, we examined the expression patterns of all syndecan isoforms in continuously growing mouse incisors, in which we can overview major differentiation stages of amelogenesis at a glance. (tokushima-u.ac.jp)
  • Immunohistochemical analysis of syndecan core proteins in the lower incisors from postnatal day 1 mice revealed spatially and temporally specific expression patterns, with syndecan-1 expressed in undifferentiated epithelial and mesenchymal cells, and syndecan-2, -3, and -4 in more differentiated cells. (tokushima-u.ac.jp)
  • Each of the syndecan core proteins has a short cytoplasmic domain that binds cytosolic regulatory factors. (embrapa.br)
  • 2023). Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. (upenn.edu)
  • ESCRTs are composed of approximately 20 proteins assembled into 4 complexes (e.g. (molcells.org)
  • PDZ domains can occur in one or multiple copies and are nearly always found in cytoplasmic proteins. (embl.de)
  • PDZ domain proteins are frequently associated with the plasma membrane, a compartment where high concentrations of phosphatidylinositol 4,5-bisphosphate (PIP2) are found. (embl.de)
  • There are 333040 PDZ domains in 224949 proteins in SMART's nrdb database. (embl.de)
  • Taxonomic distribution of proteins containing PDZ domain. (embl.de)
  • The complete taxonomic breakdown of all proteins with PDZ domain is also avaliable . (embl.de)
  • Its substrate specificity suggests that Tsp may contain a substrate recognition domain, which selectively binds to the nonpolar C-termini of substrate proteins, separate from its catalytic site. (embl.de)
  • These receptors bind various cargo proteins via their luminal Vps10p domains and have been shown to mediate a variety of intracellular sorting and trafficking functions. (biomedcentral.com)
Proteoglycans4
  • Syndecans are the best-characterized plasma membrane proteoglycans. (wikipedia.org)
  • Syndecans are transmembranous heparan sulfate proteoglycans (HSPGs) with covalently attached glycosaminoglycan side-chains located on the cell surface. (tokushima-u.ac.jp)
  • The syndecans comprise a family of heparan sulfate bearing transmembrane proteoglycans that arise from four distinct genes within mammals. (archive.org)
  • APOE associated to the LVP allows the initial virus attachment to cell surface receptors such as the heparan sulfate proteoglycans (HSPGs), syndecan-1 (SDC1), syndecan-1 (SDC2), the low-density lipoprotein receptor (LDLR) and scavenger receptor class B type I (SCARB1) (PubMed:12970454, PubMed:12356718, PubMed:12913001, PubMed:28404852, PubMed:22767607). (proteopedia.org)
Proteoglycan4
  • Syndecan-4 is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. (wikipedia.org)
  • Belongs to the syndecan proteoglycan family. (affbiotech.cn)
  • Free glycosaminoglycans (GAGs) and proteoglycan- (PG-) containing GAGs, key effectors of cell surface, pericellular and extracellular microenvironments, perform multiple functions in cancer by virtue of their coded structure and their ability to interact with both ligands and receptors that regulate cancer growth [ 1 - 4 ]. (hindawi.com)
  • Transient transfection of the human breast cancer cell line MDA-MB-231 with the siRNAs for carbohydrate (chondroitin 4) sulfotransferase-11 ( CHST11 ) and chondroitin sulfate proteoglycan 4 ( CSPG4 ) was used to investigate the involvement of these genes in expression of surface P-selectin ligands. (biomedcentral.com)
Short cytoplasmic1
  • Each subunit then has a transmembrane sequence and a short cytoplasmic tail. (novusbio.com)
Binds3
  • Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold. (wikipedia.org)
  • Syndecan-4 binds to FGF and mediates interaction with the FGF receptor. (wikipedia.org)
  • Syndecan-1, via its heparan sulfate chains, binds to a variety of extracellular matrix macromolecules including collagens, fibronectin, thrombospondin, and tenascin etc. (archive.org)
Heparan sulfate chains1
  • Syndecans have similar structural features: Attach to heparan sulfate chains - interacting factors (e.g. (wikipedia.org)
Polyadenylation element1
Epithelial2
  • Syndecans are normally found on the cell surface of fibroblasts and epithelial cells. (wikipedia.org)
  • E-Cadherin and epithelial syndecan-1 were more highly expressed in intraluminal/luminal unicystic ameloblastoma than in mural unicystic ameloblastoma and solid/multicystic ameloblastoma, whereas the stromal expression of syndecan-1 was higher in mural unicystic ameloblastoma and solid/multicystic ameloblastoma. (cusabio.com)
Cellular uptake1
  • The cellular uptake, trafficking, and nuclear localization of FGF-2 could be increased by co-delivery of syndecan-4 proteoliposomes. (wikipedia.org)
Syntenin1
  • Direct interaction between PIP2 and a subset of class II PDZ domains (syntenin, CASK, Tiam-1) has been demonstrated. (embl.de)
Mammalian1
Isoform2
  • Understanding the expression domain of each syndecan isoform during specific developmental stages seems useful for investigating their physiological roles in amelogenesis. (tokushima-u.ac.jp)
  • These findings suggest that each syndecan isoform functions distinctly during the amelogenesis of the incisors of mice. (tokushima-u.ac.jp)
Bind3
  • Also, syndecans bind to FGFs and bring them to the FGF receptor on the same cell. (wikipedia.org)
  • The I domain-containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). (novusbio.com)
  • The affinity of syndecan-1 for the matrix, its ability to bind cells to the matrix, and its pattern of expression are consistent with its proposed role as a receptor for the extracellular matrix. (archive.org)
Extracellular domains2
  • Except for the glycosaminoglycan attachment sites, the extracellular domains of the syndecans are highly divergent. (archive.org)
  • The syndecans also contain highly conserved transmembrane domains and extracellular domains for which important activities are becoming known. (embrapa.br)
Ryudocan1
  • Syndecan-4 is also called ryudocan or amphiglycan. (wikipedia.org)
Antibody1
  • Syndecan 4 Antibody detects endogenous levels of total Syndecan 4. (affbiotech.cn)
Nuclear1
  • For several substrates the liberated cytoplasmic domain has been shown to translocate to the nucleus where it is involved in nuclear signaling (Notch ErbB4, Delta-1 Jagged, APLP1/2). (biomedcentral.com)
Transmembrane domains1
  • Alignment of γ-secretase substrate transmembrane domains provides little insight into the sequence requirements for proteolysis to occur (Figure 1a ) [ 33 ]. (biomedcentral.com)
Interacts3
Receptor2
  • GDNF family receptor alpha 4 [Source. (gsea-msigdb.org)
  • The largest of the homologs, SorLA is 250 kDa and contains a host of receptor binding domains including 14 different low density lipoprotein receptor sites, and EGF repeat, and a fribronectin type III repeat. (biomedcentral.com)
Regulation2
  • The functional repertoire of heparanase is further expanded by its regulation of syndecan clustering and shedding. (pafr-signal.com)
  • Although the catalytic domain of MMPs is structurally highly similar, there are many differences with respect to substrate specificity, cellular and tissue localization, membrane binding and regulation that make this a very versatile family of enzymes with a multitude of physiological functions, many of which are still not fully understood. (evelinvahter.com)
Neurons1
  • impairing of early cancer c chromophore hydrolysis VIa neurons in cytoplasmic membrane during promoter. (evakoch.com)
Cartilage1
  • Syndecan-4 is upregulated in osteoarthritis and inhibition of syndecan-4 reduces cartilage destruction in mouse models of OA. (wikipedia.org)
Differentiation1
Species1
  • In contrast, the transmembrane and cytoplasmic domains are highly conversed both as members of the syndecan family and among different species. (archive.org)
Mediate2
  • However, there has been no direct evidence demonstrating that syndecans can mediate cell-cell adhesion. (archive.org)
  • The authors of this paper have shown the first evidence that syndecan-1 or other members of the syndecan family can mediate cell-cell adhesion. (archive.org)
Molecule1
  • The positive correlation between soluble Syndecan-1 levels and breast cancer tumor size in the present study highlights the importance of this molecule in the breast tumor progression and their significance as tumor biomarkers. (cusabio.com)
Gene1
  • The method extended the gene set enrichment analysis into the network domain, and was applied in a number of collaboration efforts. (scilifelab.se)
Tail1
  • Substrate recognition through a PDZ domain in tail-specific protease. (embl.de)
Amino4
  • The 1102 amino acid (aa) human alpha 2 extracellular domain (ECD) shares 83‑89% aa sequence identity with mouse, rat, canine, bovine and equine alpha 2. (novusbio.com)
  • PDZ domains consist of 80 to 90 amino acids comprising six beta-strands (beta-A to beta-F) and two alpha-helices, A and B, compactly arranged in a globular structure. (embl.de)
  • Systematic deletion mutagenesis of Tsp localized the binding site to amino acids 206-307, a region that completely encompasses the putative PDZ domain (217-301). (embl.de)
  • Glypicans fall into two broad subfamilies: glypicans 1/2/4/6 and glypicans 3/5 (Figure 1 ), with approximately 25% amino-acid identity between groups. (biomedcentral.com)
Focal2
  • Syndecan-4 has more widespread distribution than other syndecans and it is the only syndecan that has been found consistently in focal adhesions. (wikipedia.org)
  • p21-activated kinase 4 (PAK4), a specific effector of the Rho GTPase Cdc42, is activated by HGF, and we have previously shown that activated PAK4 induces a loss of both actin stress fibres and focal adhesions. (silverchair.com)
Abhydrolase domain2
Growth5
  • Syndecan-4 is also a regulator of fibroblast growth factor-2 (FGF-2) signaling. (wikipedia.org)
  • Because the tight correlation between syndecan-4 and growth factors, the efficiency of angiogenic therapies have been thought to relate to syndecan-4. (wikipedia.org)
  • Growth factor signaling may be disrupted by changes in syndecan-4 expression. (wikipedia.org)
  • Syndecan-4 expression is upregulated in the endometrium of women suffering from endometriosis, and its downregulation in endometriotic cells results in a decrease of invasive growth, and reduced expression of the small GTPase Rac1, Activating transcription factor 2 (ATF2), and MMP3. (wikipedia.org)
  • Inhibitors directed against the C-domain, combined with inhibitors of heparanase enzymatic activity are being developed to halt tumor growth, metastasis, angiogenesis and inflammation. (pafr-signal.com)
Sequence2
  • 2oc1 is a 4 chain structure with sequence from Hepacivirus C and Hepatitis C virus subtype 1b . (proteopedia.org)
  • 1987 ). This superfamily of proteases is defined by the presence of a Zn 2+ ion at the catalytic center, which is coordinated by three histidine residues in the zinc-binding consensus sequence HExxHxxGxxH that is present in all proteolytically active metzincins, and a characteristic, strictly conserved methionine-containing tight 1,4 beta turn forming a hydrophobic cleft for the catalytic zinc ion (Bode et al. (evelinvahter.com)
Subfamily2
Coiled-coil domain2
Cell6
  • Thus, syndecans participate in cell matrix adhesion. (archive.org)
  • In addition, it has been widely speculated that syndecans participate in cell-cell adhesion. (archive.org)
  • This is due to a number of reports indicating that syndecans are often found at sites of cell-cell contact. (archive.org)
  • The authors found that following their transfection with cDNA for syndecan-1 or syndecan-4, human B lymphoid cell line (ARH-77) forms large multicellular aggregates in suspension culture and stain intensely for the syndecans at sites of cellcell contact. (archive.org)
  • The protrusions of the cell edge are shown as green arrows corresponding to points of adhesion maturation (panels 3 and 4). (mechanobio.info)
  • Panel 4 shows sliding of adhesion and hence slight retraction of the respective section of the cell edge compared to panel 3. (mechanobio.info)
Family1
  • Metzincins are a ubiquitously expressed family of multi-domain zinc (II)-dependent endopeptidases (Stocker and Bode 1995 ), the members of which include well-known metalloproteases such as the matrix metalloproteases (MMPs) (Nagase and Woessner 1999 ), a disintegrin and metalloproteases (ADAMs) (White 2003 ), the ADAMs with a thrombospondin motif (ADAMTS) (Tang 2001 ), the bacterial serralysins (Nakahama et al. (evelinvahter.com)
Molecular2
  • Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. (wikipedia.org)
  • At chemical synapses, the presynaptic neuron forms a specialized membrane domain, termed the active zone, which contains the molecular machinery required for calcium-dependent synaptic vesicle fusion and recycling. (rupress.org)
Kinase1
Interaction2
  • AT-rich interaction domain 5A [Source:H. (gsea-msigdb.org)
  • In most cases, interaction between a PDZ domain and its target is constitutive, with a binding affinity of 1 to 10 microns. (embl.de)
Peptide4
  • The structure of PDZ domains allows binding to a free carboxylate group at the end of a peptide through a carboxylate-binding loop between the beta-A and beta-B strands. (embl.de)
  • Partial proteolysis by V8 protease at a single peptide bond immediately N-terminal to the PDZ domain resulted in two distinct and relatively stable fragments and complete loss of catalytic activity. (embl.de)
  • Site-directed mutagenesis of a surface residue at the peptide binding site of the PDZ domain, valine 229, to Glu or Gln resulted in an increase in the K(M) value but had no effect on the k(cat) value. (embl.de)
  • Following ectodomain shedding, γ-secretase cleavage liberates both the cytoplasmic fragment and a small secreted peptide. (biomedcentral.com)
Physiological1
  • It has been recognized that under physiological and pathological conditions, cells may release vesicles that may be instrumental in intercellular communication as they carry biologically active molecules ( 1 - 4 ). (spandidos-publications.com)
Highly1
  • A lead compound (non-anticoagulant glycol-split heparin), highly effective 4-Aminobutyrate aminotransferase against myeloma tumors, was selected toward a clinical trial in cancer patients. (pafr-signal.com)
Fragments1
  • Fragments of the GCX, such as syndecan-1 and/or hyaluronan (HA), have been examined, and their validity is now being examined. (biomedcentral.com)