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Receptor mobility and receptor-cytoplasmic interactions in lymphocytes. (academicinfluence.com)
An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. (lookformedical.com)

Earlier reports indicated that underhydroxylated collagen, accumulating within the endoplasmic reticulum in cells where prolyl 4-hydroxylase is inactivated, is slowly degraded, but is then rapidly hydroxylated and secreted when the activity of prolyl 4-hydroxylase is restored. (embl.de)
Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. (lookformedical.com)

Purification and characterization of a cell adhesion molecule from neural retina. (academicinfluence.com)

Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein. (academicinfluence.com)
This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)
Using a fluorescent sulfhydryl probe as well as a sulfhydryl inhibitor, one observes a strong cell density response, supporting the hypothesis that cellular redox potential could alter protein stability. (embl.de)
Greater than 90% of the protein phosphotyrosine in EGF-stimulated cells bound to the 1G2 immunosorbent (data not shown). (docksci.com)
The antibodies to substrates can then be used to study the distribution, turnover, and phosphorylation of the protein in addition to cloning the cDNA encoding the protein from an expression library. (docksci.com)

R. Glenney, Jr. and L. Zokas, J. Cell Biol. (docksci.com)

Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule. (academicinfluence.com)
Chemical characterization of a neural cell adhesion molecule purified from embryonic brain membranes. (academicinfluence.com)
Examples of a novel series of phenanthrolinones are shown to be potent competitive inhibitors of avian prolyl 4-hydroxylase, and of collagen hydroxylation, in embryonic chick tendon cells and human foreskin fibroblasts in vitro and in the oestradiol-stimulated rat uterus in vivo. (embl.de)
In embryonic avian tendon, cell density regulates collagen production. (embl.de)

Neural cell adhesion molecule: structure, immunoglobulin-like domains, cell surface modulation, and alternative RNA splicing. (academicinfluence.com)
Cell adhesion molecules. (academicinfluence.com)
Cell adhesion molecules: implications for a molecular histology. (academicinfluence.com)
Cell adhesion molecules in the regulation of animal form and tissue pattern. (academicinfluence.com)
Adhesion among neural cells of the chick embryo. (academicinfluence.com)
Cell adhesion molecules in early chicken embryogenesis. (academicinfluence.com)
Cell adhesion and the molecular processes of morphogenesis. (academicinfluence.com)
Previous studies have shown that small beads coated with FN7-10, a four-domain cell adhesion fragment of fibronectin, bind to cell surfaces and translocate rearward. (biologists.com)
Integrins are transmembrane receptors that mediate cell adhesion by forming links between the extracellular matrix and cytoskeleton. (biologists.com)

Fluorescence microscopy showed that the trimer and pentamer bound strongly to the cell surface, and within 5 minutes were prominently localized to actin fiber bundles. (biologists.com)

Surface modulation in cell recognition and cell growth. (academicinfluence.com)
On the ventral surface this rearward movement of the cytoskeleton contributes to forward motion of the cell relative to the substrate. (biologists.com)

Alternative sites for cell density regulation of the enzyme could rely on its sensitivity to sulfhydryl groups. (embl.de)

A heterogeneous family of water-soluble structural proteins found in cells of the vertebrate lens. (lookformedical.com)
We have used this procedure with good success for isolating PY proteins from chronic myelogenous leukemia cells and, with minor modifications, for isolating PY proteins from growth factor-stimulated adherent cells. (docksci.com)
Isolation of Proteins under Nondenaturing Conditions Tissue culture cells are grown on plastic 150-mm tissue culture dishes to approximately 90% confluence. (docksci.com)

To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)

Six independent candidate clones were isolated from the cDNA library screen and were identified as overlapping partial human kinectin cDNAs. (biomedcentral.com)
SLC4A9 was initially discovered by genomic sequence annotation and further characterized by sequencing of long-insert cDNA library clones. (biomedcentral.com)

cDNA corresponding to the neural antigen was cloned and sequenced. (nih.gov)
The neural cell adhesion molecule (NCAM) is a glycoprotein that modulates synaptic plasticity. (auburn.edu)
The aggressiveness of melanoma is due to the combined effects of oncogenic signaling pathways and cancer-relevant transcription factors, resulting in the transformation of neural crest (NC)-derived pigment cells (melanocytes) located in the basal layer of the skin into metastatic melanoma. (biomedcentral.com)

Clones for 4 species of ag cDNA and 1 ag genomic DNA were obtained that correspond to the ag subunit of G, signal transduction protein. (nih.gov)
Both cDNA and genomic DNA clones were obtained that correspond to species of poly At RNA that increase or decrease in abundance when cells are treated with dibutyryl cAMP. (nih.gov)

Proteins normally associated with the apical junctional complexes of epithelial cells, such as Par-3, Par-6 and atypical protein kinase C (aPKC) have a role in polarization in vitro . (biomedcentral.com)

NG108-15 cells respond to bradykinin by increased hydrolysis of phosphatidylinositol-4,5-bisphosphate and accumulation of inositol-1,4,5-trisphosphate, which releases Ca2+from intracellular stores, and diacylglycerol, which activates protein kinase C. Cytoplasmic Ca2t activates a Ca2t -dependent Kt channel leading to cell hyperpolarization. (nih.gov)

Mouse neuroblastoma-rat retina hybrid cell lines were established to rescue the expression of retinal genes. (nih.gov)
A monoclonal antibody that binds to cells from one hybrid line recognizes an antigen expressed by few cell types in retina and brain. (nih.gov)
Additional monoclonal antibodies were obtained that bind to antigens that are markers of cell types in retina. (nih.gov)
HL 00009-13 LBG Project No. Z0l HL 00009-13 LBG Major Findings: N18TG-2 mouse neuroblastoma cells were fused with 18-day rat embryo retina cells, and somatic cell hybrid lines then were established to obtain clonal cell lines that continue to express genes characteristic of cells in retina. (nih.gov)
N18RE-103 cells were used as an immunogen for the production of hybridoma cell lines that synthesize monoclonal antibodies to antigens expressed by N18RE-103 cells and retina. (nih.gov)
Thirty-three of the 374 hybridoma cell lines obtained synthesize antibodies to antigens expressed by N18RE-103 cells and rat retina cells, but not with cell lines from other tissues. (nih.gov)
Some of the antigens recognized by the monoclonal antibodies are expressed by different cell types in adult rat retina. (nih.gov)
More than a century ago, Ramón y Cajal made observations of the embryonic chick retina and drew differentiating 'RGCs' with bipolar morphologies, including a retracting apical process and an axon extending from the basal side [ 13 ]. (biomedcentral.com)

Isolated fractions were examined for their capacity to bind [125I]C1q as a measure of immune complex levels, and for their ability to bind soluble tumour-specific antigen as well as to react with antigens expressed at the surface of viable hepatoma cells. (nih.gov)

In addition, DNA clones for rat and human 4, genes were obtained. (nih.gov)
Sodium bicarbonate cotransporter (NBC) genes encode proteins that execute coupled Na + and HCO 3 - transport across epithelial cell membranes. (biomedcentral.com)
Expression profiling studies of prostate cancer cell lines revealed that multiple tumor suppressor genes are repressed by CtBP1. (nih.gov)
The action of interferons (IFNs) on virus-infected cells and surrounding tissues elicits an antiviral state that is characterized by the expression and antiviral activity of IFN-stimulated genes. (nature.com)

Antibody 89A5 binds to molecules restricted predominantly to the outer segments of photoreceptor cells. (nih.gov)
Antibody 38B6 binds to an abundant antigen on photoreceptor cell bodies and to less abundant antigen associated with horizontal, bipolar, and amacrine neuron soma. (nih.gov)
AB - Antibody activity to Herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) was measured by the indirect hemagglutination (IHA) test in sera from 124 women with squamous-cell carcinoma of the uterine cervix, 46 women with non-cervical cancer and 116 matched normal women. (nih.gov)

The predicted COOH terminus is similar to that of glycosylphosphatidylinositol-linked proteins, and antisera raised to this region react with in vitro translation products of the cDNA clone but not with mature beta-tectorin. (embl.de)
AB - Peripheral blood mononuclear cells from donors of known serological status with respect to EB virus were exposed to the virus in vitro and then cultured at various cell concentrations. (nih.gov)
The results strongly suggest that the regression phenomenon is an in vitro expression of long-term T-cell-mediated immunity to EB virus which the large majority, if not all, infected individuals possess. (nih.gov)

The cDNA and deduced amino acid sequences for chick beta-tectorin are presented. (embl.de)

Increasing amounts of research evidence supports the possibility that it is an immune-mediated vasculitis, and that abnormal T-cell and B-cell reactions and autoantigen-driven autoimmunity play pivotal roles [ 1 ]. (biomedcentral.com)
Most types of cell can produce IFN-α and IFN-β, which are the best-characterized type I IFNs, whereas IFN-γ is produced only by certain cells of the immune system, including natural killer (NK) cells, CD4 + T helper 1 (T H 1) cells and CD8 + cytotoxic T cells. (nature.com)
Cancer is a disease that involves multiple cells - many cancer cells, as well as blood vessel cells, immune cells, etc. (nih.gov)

In order to address this, we performed a high-resolution messenger RNA differential display screen to identify molecules whose expression is regionally restricted along the anteroposterior (AP) neuraxis during early chick development, with particular focus on the midbrain and hindbrain vesicles. (biomedcentral.com)
We have now identified one of the major autoantibody specificities using expression cloning. (biomedcentral.com)
AlamarBlue, transwell invasion and colony formation assays in melanoma cell lines were conducted to investigate the epistatic relationship between SOX10 and NEDD9, as well as the effects of graded SOX9 expression levels. (biomedcentral.com)
Accordingly, SOX10 was required for NEDD9 expression, which partly mediated its oncogenic functions in melanoma cells. (biomedcentral.com)
Compensatory upregulation of SOX9 expression in SOX10-inhibited melanoma cells reduced growth and migratory capacity, partly due to elevated expression of cyclin-dependent kinase inhibitor p21 and lack of NEDD9 induction. (biomedcentral.com)
In addition, overexpression of SOX9 can also promote invasiveness of the parental melanoma cells by modulating the expression of various matrix metalloproteinases. (biomedcentral.com)

All cultures from nine seronegative adult and 12 foetal donors gave rise to cell lines following subculture 4 weeks post infection. (nih.gov)
In 1996, the genome of an adult sheep mammary cell was injected into an unfertilized developing sheep egg cell, resulting in the birth of a cloned sheep. (nih.gov)

Monospecific antibodies to an subunit of voltage-sensitive Ca2+ channels from rat T-tubules were shown to activate voltage-sensitive calcium channels of parathyroid cells. (nih.gov)
Unlike the picture in SLE and other related rheumatic diseases, in BD, antinuclear antibodies and antibodies to neutrophil cytoplasmic antigens etc. are not present. (biomedcentral.com)
PMID- 214407 TI - Antibodies to Herpes simplex virus types 1 and 2 in patients with squamous-cell carcinoma of uterine cervix in India. (nih.gov)

We find that the situation is indeed very different in vivo , where axons emerge directly from uniformly polarized cells in the absence of other neurites. (biomedcentral.com)

The concentration of nutrients and other molecules is typically much higher inside the cell, thus making the reactions required for cell function more efficient. (nih.gov)

In dissociated cell cultures, neurons develop in the presence of very scarce external cues, and so must perforce break symmetry intrinsically. (biomedcentral.com)
In contrast, seropositive donor cultures seeded at the higher cell concentrations developed foci of proliferating EBNA-positive cells within the first 1--2 weeks but thereafter regressed completely and subcultures made after 4 weeks never gave rise to cell lines. (nih.gov)
Out of 18 seropositive donors tested, 15 showed regression in all cultures seeded at 10(6) cells/ml and above, and with the other three donors a proportion of replicate cultures regressed. (nih.gov)
T-cell depletion and reconstitution experiments showed that the effect was absolutely dependent upon the presence in the cultures of T cells from these seropositive donors. (nih.gov)

The global antiviral state of the cell involves cross-talk between IFN signalling and pathways that regulate apoptosis, inflammation and cellular stress-response programmes. (nature.com)

whereas, tyrosine hydroxylase is not expressed by the parental N18TG-2 cells. (nih.gov)

Furthermore, our studies indicate a role for CtBP1 in conferring radiation resistance to prostate cancer cell lines. (nih.gov)

Transient and stable knockdown of CtBP1 in prostate cancer cells inhibited their proliferation and invasion. (nih.gov)

The etiopathogenesis of the disease remains unclear but microbial agent triggers, environmental factors, genetic predisposition, neutrophil hyperfunction, endothelial cell dysfunction and immunological abnormalities involving both T and B cells have been implicated. (biomedcentral.com)

Joe Gindhart: Cells keep the inside in (DNA, enzymes, organelles, biochemical molecules) and the outside out (the environment). (nih.gov)

Interferons (IFNs) - the body's first line of antiviral defence - are cytokines that are secreted by host cells in response to virus infection. (nature.com)

It is currently represented by ten kidney clones and one testis clone from the IMAGE consortium (Unigene: Hs.166669). (biomedcentral.com)
Two expressed sequence tag (EST) clones (IMAGE: 1734773 and 1533693) were sequenced to completion, yielding a 1,036 base pair (bp) cDNA contig. (biomedcentral.com)

Francis Howell Central High School, Missouri: Are there any scientists designing novel organs/body structures or cell types? (nih.gov)

Cell Recognition and Synapse Formation fRINcl ey VegTiGar R (List other rere ional personnel below the Principal investigator. (nih.gov)

To investigate this, we use four-dimensional microscopy of developing retinal ganglion cells (RGCs) in live zebrafish embryos. (biomedcentral.com)
Live cell imaging was conducted to monitor dynamics of melanoma migratory behavior. (biomedcentral.com)

If hippocampal cells are cultured soon after their final mitotic divisions, multiple neurites emerge simultaneously at seemingly random orientations. (biomedcentral.com)

Co-op High School New Haven, Connecticut: Why do normal cells become cancer cells? (nih.gov)
With successive rounds of cell division, more mutations can accumulate eventually transforming a normal cell into a cancer cell. (nih.gov)

Fully differentiated skeletal muscle fibers are multinucleated, elongated cells with highly ordered cytoplasm, essential for efficient muscle contraction and organismal movement. (silverchair.com)

IFNs exert their biological functions by binding to specific cell-surface receptors. (nature.com)

PMID- 214405 TI - Long-term T-cell-mediated immunity to Epstein-Barr virus in man. (nih.gov)

Anatomy46

Organisms20

Diseases4

Chemicals and Drugs81

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Psychiatry and Psychology1

Phenomena and Processes75

Disciplines and Occupations1

Information Science4

Lymphocytes2

Endoplasmic reticulum2

Purification1

Protein5

Biol1

Embryonic4

Adhesion9

Bound1

Surface2

Regulation1

Proteins3

Human1

CDNA library2

Neural3

Genomic2

Epithelial cells1

Intracellular1

Retina8

Antigens expressed1

Genes4

Antibody3

Vitro3

Amino1

Immune3

Expression6

Adult2

Antibodies3

Vivo1

Reactions1

Cultures4

Involves1

Parental1

Lines1

Invasion1

Triggers1

Molecules1

Infection1

IMAGE2

Types1

Formation1

Live2

Multiple1

Normal2

Efficient1

Functions1

Virus1