Cytoplasmic and nuclear maturation vitro oocyte nuclear maturation in vitro oocyte maturation techniques. Medical search. Frequent questions

The present review discusses methods to improve oocyte quality in older women and new stimulation protocols that may improve the number of mature oocytes retrieved during an in vitro fertilization cycle. (arvigen.com)
To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)

They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
In vivo development was assessed after transfer of injected oocytes to the oviducts of recipient mares. (bioone.org)
The application of reproductive biotechnology tools such as estrus synchronization (ES), artificial insemination (AI), embryo transfer (ET) through multiple ovulation and in vivo collection of embryos, in-vitro embryo production (IVEP) and cryopreservation, somatic cell nuclear transfer (SCNT) to produce the desired breed and sex of animal, and other reproductive biotechnologies that enhances reproduction potential are important innovations that can address specific needs and purpose for animal reproduction. (ifory.id)
Components and OPTIONS FOR in vivo test: In vitro maturation and fertilization maturation and fertilization had been carried out as described [21] previously. (bioskinrevive.com)
For in vivo test: In vitro lifestyle and embryo freezing After IVF, oocytes encircled with cumulus cells had been placed in fresh new TCM-199 moderate, as well as the embryos had been co-cultured with cumulus cells, as previously defined [21]. (bioskinrevive.com)
This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
didemnin b (db) is a cyclic peptide with potent immunosuppressive activity in vitro and in the murine graft-versus-host-reaction (gvhr), the only measure of in vivo immunity tested in our prior studies. (liverpool.ac.uk)

In this paper, we describe a scanning electrochemical microscopy (SECM) technique that is a non-invasive and sensitive method for measuring oxygen consumption by individual embryos. (bioone.org)
SECM technique may be a valuable tool for accurately assessing the quality of embryos and thereby contribute to improving outcomes associated with assisted reproduction, including human in vitro fertilization. (bioone.org)
MicroRNAs (miRNAs) are small non-encoding RNAs that actively regulate biological and physiological processes, and play an important role in regulating gene expression in all cells, especially in most animal cells, including oocytes and embryos. (bvsalud.org)
Although assisted reproductive techniques (ART) have helped to solve many infertility problems, they cause changes in the expression of miRNA and genes in oocytes and preimplantation embryos, and the effect of these changes on the future of offspring is unknown, and has caused concerns. (bvsalud.org)
The blastocyst stage of oocyte developed embryos was reached by 12.43±2.15% and 22.28±4.86% of the control and treatment groups, respectively. (sciencegate.app)
ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. (sciencegate.app)
Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
oxidative stress is the most prominent one, which will cause damage or alter the genetic material of the sperm and reduce the quality of the oocytes, and has a crucial impact on the possibility of developing embryos even after implantation. (vetmedmosul.com)
Oocytes, sperms, and embryos are shielded from oxidative stress in their natural environment by free radical scavengers found in oviductal and follicular fluids. (vetmedmosul.com)
Oocytes and embryos are not provided with this level of protection when in vitro production (9). (vetmedmosul.com)
Melatonin supplementation has been shown to promote bovine and porcine oocytes' maturation rate and increase their blastocyst of IVF embryos (16). (vetmedmosul.com)

Matured oocytes had been inseminated with frozen-thawed semen from order MK-0822 a Japanese Dark bull (altered to 2 107 cells/ml) for 5 h at 38.5C within a humidified atmosphere with 5% CO2 in the surroundings, in 1 ml of BO solution containing 10 mg/ml bovine serum albumin and 10 g/ml heparin. (bioskinrevive.com)
It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro. (sciencegate.app)

cRNA microinjection of full-length or truncated MAD2L1BP uncovered their discordant roles in driving the extrusion of polar body 1 (PB1) in mouse oocytes. (bvsalud.org)

After that, oocytes are collected by slicing and only oocytes with a cytoplasmic are homongent and have compact cumulus cells used in the study. (ifory.id)
First, matured oocytes were evaluated its morphology based on the expansion of cumulus cells and PB1 extrusion. (sciencegate.app)

Currently, its estimation is predominantly based on morphological analysis, which is subjective and does not consistently reflect the developmental competence of the oocytes. (biomedcentral.com)
however, it is not per se sufficient to assure oocyte developmental competence [ 13 ]. (biomedcentral.com)

Xenopus oocytes are encompassed by a layer of follicular cells that contribute to oocyte growth and meiosis in relation to oocyte maturation. (bvsalud.org)
Here, we investigated Xenopus follicular cell function using oocyte signaling and heterologous-expressing capabilities. (bvsalud.org)
Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. (biologists.com)
an investigation of their specific localization in human brain and their influence on CaV2.1 voltage-dependent calcium channels expressed in Xenopus oocytes. (biomedcentral.com)
In addition, we investigated whether human γ 2 or γ 4 could modulate the electrophysiological properties of a neuronal VDCC complex transiently expressed in Xenopus oocytes. (biomedcentral.com)
When co-expressed in Xenopus oocytes with a Ca V 2.1/β 4 VDCC complex, either in the absence or presence of an α2δ 2 subunit, neither γ 2 nor γ 4 significantly modulated the VDCC peak current amplitude, voltage-dependence of activation or voltage-dependence of steady-state inactivation. (biomedcentral.com)

Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)

ROS are free radicals that are continuously produced by oxidative metabolic reactions and increase in vitro conditions, causing aging of both the oocyte (7) and sperm (8), as well as perhaps impairing fertilization and embryo development. (vetmedmosul.com)

However, the effects of the interaction between follicular cells and the oocyte surface on meiotic processes are unclear. (bvsalud.org)
We found that oocytes deprotected from their surrounding layer of follicular cells and expressing the epidermal growth factor (EGF) receptor (EGFR) and the Grb7 adaptor undergo accelerated prophase I to metaphase II meiosis progression upon stimulation by EGF. (bvsalud.org)
In addition, we determined that oocytes surrounded by their follicular cells expressing EGFR-Grb7 exhibit normal meiotic resumption. (bvsalud.org)
Furthermore, an O-GlcNAcylation increase (by inhibition of O-GlcNAcase), the glycosidase that removes O-GlcNAc moieties, or decrease (by inhibition of OGT) amplifies oocyte spindle defects when follicular cells are absent highlighting a control of the meiotic spindle by the OGT-O-GlcNAcase duo. (bvsalud.org)
In summary, our study provides further insight into the role of the follicular cell layer in oocyte meiosis progression. (bvsalud.org)
In affected ovaries, oxygenation of follicular fluid is low, ultrastructural disturbances especially of mitochondria, occur in granulosa cells and oocytes, and considerable disturbances of meiosis occur. (biomedcentral.com)
As well as inducing a plethora of deleterious changes in follicular cytoplasmic structure and function, the omega 9 palmitate/oleate ratio is increased by lowered activity of PPARα. (biomedcentral.com)

These microinsemination techniques can be combined with cryopreservation of sperm and spermatid cells, leading to successful transmission of transgenes carried in male gametes. (go.jp)
Furthermore, if it can be demonstrated that the biochemical and structural basis upon which memory and personality (personal identity) rest are degraded or destroyed by some cryopreservation techniques, while being preserved by others, then the issue of what treatment to choose within the sphere of human cryopreservation procedures becomes paramount. (chronopause.com)

In conclusion, melatonin could be a promising tool for improving sperm competence for fertilizing oocytes and embryo development in sheep. (vetmedmosul.com)

When the inability of male transgenic rats to transmit the transgene is not associated with mosaicism, microinsemination techniques such as intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI) can be used for passage of the transgenes. (go.jp)

The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
Photographs have been quantified working with ImageJ program and taking into consideration different acceptable approaches, We set to scale the pixel ratio to microns and utilized measurement examination to quantify the location occupied by autophagosome and autolysosomes as when compared with the complete cytoplasmic place excluding the nucleus. (narturalproduct.com)
The bound complex is internalized and translocates into the oocyte nucleus thanks to an SV40 nuclear localization signal, enhanced by an adjacent casein kinase phosphorylation site. (biologists.com)
In the oocyte, mitochondria play a central role in cellular metabolism providing energy for successful cytoplasm and nucleus maturation, fertilization, embryo development, calcium homeostasis, and apoptosis initiation. (biomedcentral.com)

in 2015 showed that supplementation of CoQ10 in an aged animal model delayed decline in ovarian reserve, restored oocyte mitochondrial gene expression, and improved mitochondrial activity with a significant reduction in oocyte aneuploidy. (arvigen.com)
Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)
Since recent clinical studies have indicated that dehydroepiandrosterone (DHEA) supplementation may reverse the problem of oocyte quality, this review of the literature was undertaken in an attempt to find an explanation of why this is effective? (biomedcentral.com)

As a result of spermatogenesis and epididymal maturation, male germ cells differentiate to become fully functional spermatozoa. (biomedcentral.com)
On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential cellular functions including the function of germ cells (oocytes and spermatozoa) and thereby the fertilization process. (hindawi.com)
However, these techniques have to be carried out very carefully as they, if applied wrongly, bear risks of generating ROS damaging the germ cells and preventing fertilization. (hindawi.com)

The in vitro embryo production (IVEP) is very stressful for gametes. (vetmedmosul.com)
As a result, developing a culture medium capable of effectively reducing ROS in vitro embryo production (IVEP) protocols is of tremendous importance (10). (vetmedmosul.com)

One known cause is the retention of excess cytoplasm around the midpiece due to an arrest in spermiogenesis and incomplete cytoplasmic extrusion [ 1 ]. (biomedcentral.com)
Furthermore, the patient's oocytes carrying the mutated MAD2L1BP resumed polar body extrusion (PBE) when rescued by microinjection of full-length MAD2L1BP cRNAs. (bvsalud.org)

Recent studies have shown cytoplasmic droplets to be normal morphological occurrences in human male spermatozoa. (biomedcentral.com)

The cytoplasmic droplet then becomes known as excess residual cytoplasm, which can impair overall sperm function and produce higher levels of reactive oxygen species, potentially leading to male infertility. (biomedcentral.com)
In addition, other corrective measures include sperm separation for assisted reproductive techniques. (hindawi.com)
Our ICSI procedure includes the use of a piezo-driven small-sized injection pipette (2-4 μm in diameter), isolated sperm heads, and freshly ovulated oocytes. (go.jp)
In other words, a sperm cell found in the caput of the epididymis possesses characteristics that are different from sperm cells found in the corpus or cauda of the same epididymis, such as the cytoplasmic droplet position. (encyclopedia.pub)
A better understanding of the function of this organ and of the chronology of sperm maturation may elucidate the etiological background of many idiopathic subfertilities that are related to disorders in sperm maturation. (encyclopedia.pub)
In the initial segment of the epididymis, the sperm cell is immotile-unable to fertilize an oocyte and has a proximal cytoplasmic droplet-close to the neck [ 3 ] . (encyclopedia.pub)
For this purpose, we have incubated ram sperm using four different treatments in terms of antioxidants: melatonin, cysteamine, vitamin C, and vitamin E. They were incubated by the standard methods of maturation and capacitation of sperm. (vetmedmosul.com)
In the last decade, in vitro fertilization and sperm capacitation techniques have significantly advanced. (vetmedmosul.com)
To fertilize the oocytes, sperm must undergo a series of physiological and biochemical alterations in vitro (3). (vetmedmosul.com)
Oxidative stress is one of the key factors contributing to a decrease in sperm quality during the maturation and capacitation of the sperm (5). (vetmedmosul.com)
thereby, oocytes and sperm are highly vulnerable to reactive oxygen species (ROS) attacks, affecting their quality and viability (6). (vetmedmosul.com)

Though the distinction between cytoplasmic droplets and excess residual cytoplasm has been made, some studies fail to recognize the difference and incorrectly label the latter as a cytoplasmic droplet. (biomedcentral.com)

The decreased quality of oocytes involves an increased rate of chromosomal aneuploidy with aging predominantly related to meiotic errors during oocyte maturation. (arvigen.com)
These oocytes are protected from abnormal meiotic spindle formation through the recruitment of O-GlcNAcylated Grb7, and OGT (O-GlcNAc transferase), the enzyme responsible for O-GlcNAcylation processes, in the integrin ß1-EGFR complex. (bvsalud.org)
As atresia continues, both the number and quality of oocytes fall below a critical level, and the rate of aneuploidy increases - a finding that is related at least in part to problems of the meiotic spindle 15 , 16 resulting in nondisjunction. (ldysinger.com)

Understanding the biological functions of miRNAs in frozen maturated oocytes may provide a better understanding of embryonic development and a comparison of fertility conservation in female mammals. (bvsalud.org)
The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)

Illustration comparing ( A ) spermatozoa with typical cytoplasmic droplets (CD) and ( B ) spermatozoa presenting with excess residual cytoplasm (ERC). (biomedcentral.com)
After maturation, in vitro fertilization was carried out using TALP media with a final concentration of spermatozoa 1.5 x 106 spermatozoa/ml. (ifory.id)
The oocytes were fertilized by spermatozoa that had been capacitated with two groups of fertilization media, the first group containing melatonin and the second group containing cysteamine. (vetmedmosul.com)

Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)
In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. (sciencegate.app)
The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)

Using in vitro and cellular techniques, we present a mVP40 assembly model highlighting two distinct oligomerization interfaces: the (N-terminal domain (NTD) and C-terminal domain (CTD)) in mVP40. (uci.edu)

These findings are linked to melatonin's ability to reduce ROS and its adverse effects on oocytes, such as aging, apoptosis, and its capacity to bind to specific DNA sequences and interact with cytosolic molecules to regulate antioxidant genes (17). (vetmedmosul.com)

Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)

In this study, by whole-exome sequencing (WES), we identified homozygous and compound heterozygous MAD2L1BP variants in three families with female patients diagnosed with primary infertility owing to oocyte metaphase I (MI) arrest. (bvsalud.org)
Oocytes were considered mature when they reached metaphase II. (sciencegate.app)

We have previously shown that fish primary preadipocytes differentiate into mature adipocytes in vitro and that these cells represent a very helpful model system to study adipose tissue development in fish [ 5 , 6 ]. (biomedcentral.com)

Background: The purpose of this study was to evaluate the effectiveness and possible complications of the laparoscopic ovum pick-up (LOPU) technique and to optimize ovarian stimulation in recently weaned ewe lambs. (unesp.br)
This study aims to determine the ability of in vitro embryo cleavage of Bali cattle 48 hours after the fertilization process based on different ovarian reproductive status. (ifory.id)
This case of discordant twins is not unique, however, and nine other twin pairs have subsequently consulted our center for ovarian transplantation in preference to conventional oocyte donation . (infertile.com)

The expression of miRNAs at the right time and place is crucial for the oocyte's maturation and the embryo's subsequent development. (bvsalud.org)

The average number of visualized and aspirated follicles, retrieved oocytes and the oocyte recovery rates for G80U and G160U were similar to those reported by studies in adult sheep. (unesp.br)
It is thus suggested that weaned ewe lambs may require high concentrations of exogenous FSH to produce a higher number of better quality follicles and to achieve superior in vitro maturation rates. (unesp.br)
These protease-induced decreases in IGFBP-4 and -5 likely cause increased levels of bioavailable (or free) IGFs that stimulate steroidogenesis and mitogenesis in developing dominant follicles, which ultimately prepare the follicle(s) and oocyte(s) for successful ovulation and fertilization. (bioone.org)

This unusual maturation unravels atypical spindle formation but is rescued by inhibiting integrin ß1 or Grb7 binding to the EGFR. (bvsalud.org)
Works in various organisms have revealed that the kinase is involved in centrosome separation, duplication and maturation as well as in bipolar spindle assembly and stability. (lookformedical.com)

A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)

Many investigations have been designed to gain new insights into the mechanisms underlying oocyte quality based on a differential analysis applied to molecular, biochemical, transcriptomic, or proteomic data. (biomedcentral.com)

Several physiological parameters, such as viability, mitochondrial activity, and reactive oxygen species (ROS) levels have been also reported as useful markers of oocyte quality in marine invertebrates. (biomedcentral.com)

Quantitative and qualitative assessment of retrieved oocytes were carried out after LOPU. (unesp.br)
Hence, to date, an effective and simple proxy of oocyte quality parameters has not been established yet making difficult for an accurate assessment of the female gamete quality before fertilization. (biomedcentral.com)
Nevertheless, the complexity of the techniques applied to assess gene expression and protein profile is a major bottleneck for the use of molecular markers in the oocyte quality assessment [ 12 ]. (biomedcentral.com)

The fact that live-birth rates from oocyte donation in older women are consistent with the age of the donor suggests that oocyte quality is the major factor responsible for reduced fecundability with aging. (arvigen.com)

Oocytes are highly specialized cells that undergo the unique processes of meiosis and fertilization and execute a molecular program for development [ 1 ]. (biomedcentral.com)

Oocyte quality has been defined as the ability of oocytes to be fertilized and produce viable progeny. (biomedcentral.com)

Moreover, the in vitro experiment demonstrated high glucose amounts' deleterious effect on embryo development [ 3 ]. (ejast.org)

As a result, these aged mice had more oocytes with stimulation, the developmental potential of the oocytes was improved, and more offspring were born compared to old animals receiving placebo 1. (arvigen.com)

Human oocyte maturation arrest represents one of the severe conditions for female patients with primary infertility. (bvsalud.org)
Together, our studies identified and characterized novel biallelic variants in MAD2L1BP responsible for human oocyte maturation arrest at MI, and thus prompted new therapeutic avenues for curing female primary infertility. (bvsalud.org)

The selected oocytes are then matured in an incubator of 5% CO2, a temperature of 38.5 °C for 24 hours. (ifory.id)
Oocytes 5-6 after fertilization were washed 3 times using CR1aa media then cultured in 5% CO2 incubator, temperature 38.5 °C for 48 hours. (ifory.id)

Induced totipotent cells can be obtained by reprogramming somatic cells with somatic-cell nuclear transfer (SCNT). (wikipedia.org)
hESCs can be generated by SCNT using dermal fibroblasts nuclei from both a middle-aged 35-year-old male and an elderly, 75-year-old male, suggesting that age-associated changes are not necessarily an impediment to SCNT-based nuclear reprogramming of human cells. (wikipedia.org)
Somatic Cell Nuclear Transfer (SCNT-Cloning) is a promising technique in many areas and is based on genetically identical individuals. (pvb.com.br)

Despite several recent studies investigating molecular markers related to oocyte quality, methods currently available for their determination pose various technical challenges and limitations. (biomedcentral.com)

Other attempts to overcome ooplasmic aging have utilized oocyte manipulation at a subcellular level. (arvigen.com)

This has been shown for intrauterine insemination (IUI), in vitro fertilization (IVF), and ICSI, and increased incidences of chromosomal abnormalities, minor and major birth defects, or early childhood cancers, particularly in the male offspring, have been linked to these procedures [ 8 - 16 ]. (hindawi.com)

Decline in the Number of Oocytes from Birth to Menopause. (ldysinger.com)

This study aimed to determine the influence of antioxidants on the achievement of In vitro culture (IVC) and sperm's ability to adhere to and penetrate further into In vitro maturated oocytes. (vetmedmosul.com)

However, in this group, the use of the available techniques is controversial and should be considered experimental. (ldysinger.com)
When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)

Anatomy9

Organisms3

Chemicals and Drugs5

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes7

Mature oocytes3

Vivo9

Embryos11

Bovine2

Mouse oocytes1

Cumulus cells2

Developmental competence2

Xenopus6

Injected with frozen-thawed1

Fertilization and embryo development1

Follicular7

Cryopreservation2

Competence1

Intracytoplasmic1

Nucleus4

Supplementation3

Germ cells3

Embryo production2

Extrusion2

Morphological1

Sperm11

Droplet1

Meiotic3

Embryonic development2

Spermatozoa3

COCs3

Cellular1

Apoptosis1

Conducted to evaluate1

Metaphase2

Differentiate1

Ovarian3

Oocyte's1

Follicles3

Spindle2

Involves1

Biochemical1

Physiological1

Assessment3

Donor1

Meiosis1

Viable1

Deleterious1

Stimulation1

Arrest2

38.52

SCNT3

Quality1

Manipulation1

ICSI1

Decline1

Achievement1

Experimental2