The purpose of this study was to identify membrane proteins of PMNs, and/or glomerular cells, as additional autoantigenic ANCA targets. (rupress.org)
Using these probes, evidence was provided that gp170/80-110 is identical with human lysosomal-associated membrane protein 2 (h-lamp-2), because both proteins were immunologically cross-reactive and screening of a cDNA expression library from human promyelocytic leukemia cells with anti-gp130 Ab yielded a clone derived from h-lamp-2. (rupress.org)
Dashed boxes indicate the proteins that function in flagellar secretion, either in the membrane-bound part of the apparatus or in delivery of substrate. (nature.com)
The cytoplasmic membrane proteins ExbB and ExbD support TonB-dependent active transport of iron siderophores and vitamin B12 across the essentially unenergized outer membrane of operon, where mutations in either gene produce the same phenotype: loss of approximately 90% of TonB-dependent activity (2, 5, 10, 40). (informationalwebs.com)
prominent soluble domains of each protein could serve as conversation sites with other proteins: perhaps TonB in the case of ExbD and unknown cytoplasmic proteins in the case of ExbB. (informationalwebs.com)
We hypothesized that the DCs ability to capture, internalize, and process integral membrane proteins would vary based on the target cell's viability and that the DCs ability to capture cell-associated protein would vary based on the protein's intracellular localization. (pitt.edu)
The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. (cipsm.de)
This reagent extracts cytoplasmic, nuclear and membrane proteins. (abcam.com)
The Coulton research group studies membrane proteins (MPs) that are required for transport of iron, an essential nutrient, across the bacterial cell envelope. (mcgill.ca)
TonB, partner protein of FhuA, is one of three proteins from the energy-transducing complex TonB-ExbB-ExbD that is embedded in the cytoplasmic membrane. (mcgill.ca)
This report describes Ca2+-dependent binding of 125I-labeled calmodulin (125I-CaM) to erythrocyte membranes and identification of two new CaM-binding proteins. (jhu.edu)
In the following, we refer to pump of halophilic archaebacteria, has become the these as the extracellular (EC) channel and the paradigm of membrane proteins in general and ion cytoplasmic (CP) channel. (lu.se)
Vesicle4
The lipid bilayer surrounding a cytoplasmic vesicle. (planteome.org)
Link to all annotated objects annotated to cytoplasmic vesicle membrane. (planteome.org)
Link to all direct and indirect annotations to cytoplasmic vesicle membrane. (planteome.org)
We have previously used in vivo formaldehyde cross-linking to examine TonB interactions with the outer membrane receptor FepA and with ExbB (24, 26, 40). (informationalwebs.com)
To quantitatively and qualitatively characterize uptake, we created a biologically relevant system using the Epstein Barr virus latent membrane protein 2 and the melanoma protein gp100, each fused to the enhanced green fluorescent protein (EGFP) and expressed at the outer plasma membrane of a tumor cell line, along with a cell line expressing EGFP in the cytoplasm. (pitt.edu)
However, scattering originating from flagella was replaced by a term accounting for the oligosaccharide cores of the lipopolysaccharide leaflet of the outer membrane including its cross-term with the cellular body. (iucr.org)
Specifically, this approach provides detailed insight into structural features of the cellular envelope, including the distance of the inner and outer membranes, as well as the scattering length densities of all bacterial compartments. (iucr.org)
In this study, we use cryo-electron tomography (cryo-ET) and high-speed atomic force microscopy (HS-AFM) to directly visualize the pepD2M-induced disruption of the outer and inner membranes of the Gram-negative bacterium Escherichia coli, and compared with a well-known pore-forming peptide, melittin. (bvsalud.org)
1998) observed that the outer hair cell cytoplasmic Ca2+ concentration rises following acoustic overstimulation. (cdc.gov)
Antineutrophil2
Necrotizing and crescentic glomerulonephritis (NCGN) is frequently associated with circulating antineutrophil cytoplasmic autoantibodies (ANCA). (rupress.org)
Antinuclear antibody and antineutrophil cytoplasmic antibody assays were negative. (who.int)
Basement membrane3
30 nm in diameter, establish direct membrane-to-membrane contact with endothelial cells in specialized areas of irregular endothelial basement membrane and enriched with vesicular activity. (elifesciences.org)
Renal biopsy revealed membranoproliferative glomerulonephritis, with lobulated glomerular tufts, endocapillary proliferation, thickened basement membrane, and glomerular hyaline thrombi suggestive of cryoglobulin deposits. (who.int)
Immunofluorescence showed diffuse deposition of glomerular basement membrane immunoglobulin (Ig)M and complement component C3, with mesangial IgG, but negative for C4, fibrin and IgA. (who.int)
Periplasmic2
Some ICM vesicles have no connection with other structures, others are found nearer to the cytoplasmic membrane (CM), often forming interconnected structures that retain a connection to the CM, and possibly having access to the periplasmic space. (nih.gov)
We demonstrate that the IMs of Escherichia coli and Yersinia pseudotuberculosis are asymmetric, with a 75%/25% (cytoplasmic/periplasmic leaflet) distribution of phosphatidylethanolamine (PE) in rod-shaped cells and an opposite distribution in E. coli filamentous cells. (duke.edu)
Soluble2
This protein can be a membrane-bound or soluble protein. (nih.gov)
Finally, alternative splicing gives rise to three different isoforms of CD1c (soluble, membrane, and cytoplasmic/soluble isoforms). (fishersci.com)
Endothelial cells2
A novel class of autoantigens of anti-neutrophil cytoplasmic antibodies in necrotizing and crescentic glomerulonephritis: the lysosomal membrane glycoprotein h-lamp-2 in neutrophil granulocytes and a related membrane protein in glomerular endothelial cells. (rupress.org)
By contrast, gp130 was localized in the surface membranes of endothelial cells of human glomerular and renal interstitial capillaries, rather than in lysosomes, as found for h-lamp-2. (rupress.org)
Granule1
Gp170/80-110 was localized primarily in granule membranes of resting PMNs, and was translocated to the cell surfaces by activation with FMLP. (rupress.org)
Mammalian cells1
It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells. (biotium.com)
Host cell membrane3
As an enveloped virus, EBV must fuse with a host cell membrane for entry and infection by using glycoproteins gH/gL, gB, and gp42. (uic.edu)
At the site of bacterial attachment, the commonly has sIgA against various EHEC lipopolysaccha- host cell membrane forms a pedestal-like structure. (cdc.gov)
Intracellular3
The data in this paper identify a histidine-rich sequence within the cytoplasmic domain of the nonerythroid anion exchanger, AE2, that serves as an intracellular pH 'sensor' that modulates anion exchange activity within the physiological range of cytoplasmic pH. (bgu.ac.il)
These data reveal an interaction between the two major domains of the anion exchanger and suggest a novel substrate feedback mechanism by which intracellular protons directly control the activity of an acid-loading plasma membrane ion transporter. (bgu.ac.il)
Polymyxin B damages bacterial cytoplasmic membrane and alters permeability, causing intracellular constituents to leak. (medscape.com)
Glycoprotein4
When membrane protein fractions were prepared from PMNs and isolated human glomeruli, and immunoblotted with ANCA sera of NCGN patients, two bands with apparent molecular masses of 170 and 80-110 kD (gp170/80-110) were labeled in PMNs, and a 130-kD glycoprotein (gp130) in glomeruli. (rupress.org)
The Epstein-Barr virus (EBV) glycoprotein B cytoplasmic C-terminal tail domain regulates the energy requirement for EBV-induced membrane fusion. (uic.edu)
The entry of enveloped viruses into host cells is preceded by membrane fusion, which in Epstein-Barr virus (EBV) is thought to be mediated by the refolding of glycoprotein B (gB) from a prefusion to a postfusion state. (uic.edu)
To further characterize the function of the EBV gB cytoplasmic C-terminal tail domain (CTD) in fusion, we used a previously constructed CTD truncation mutant and studied its fusion activity in the context of other EBV glycoprotein mutants. (uic.edu)
Complex2
The γ-secretase complex, comprising presenilin 1 (PS1), PEN-2, APH-1 and nicastrin, is a membrane-embedded protease that controls a number of important cellular functions through substrate cleavage. (nature.com)
γ-secretase is a membrane protein complex comprised of presenilin, nicastrin, Aph-1, and Pen-2. (nature.com)
Antibody1
Radioisotopes and the Renaissance chemiluminescence immunoblot kit were purchased from NEN Life Sciences (Boston, Mass.). Immobilon-P polyvinylidene difluoride (PVDF) membrane was purchased from Millipore Corp. (Bedford, Mass.). Anti-T7 monoclonal antibody, pET expression system, and DE3 lysogenization kit were purchased from Novagen, Inc. (Madison, Wis. (informationalwebs.com)
Cell11
Small indents of the CM are also seen, which are proposed to give rise to the UPB precursor membranes upon cell disruption. (nih.gov)
Sekler, I , Kobayashi, S & Kopito, RR 1996, ' A cluster of cytoplasmic histidine residues specifies pH dependence of the AE2 plasma membrane anion exchanger ', Cell , vol. 86, no. 6, pp. 929-935. (bgu.ac.il)
We show here that a splice variant of human thioredoxin reductase 1, TXNRD1_v3, forms both dynamic cytoplasmic filaments and provokes instantaneous formation of dynamic cell membrane protrusions identified as filopodia. (houstonmethodist.org)
We conclude that the glutaredoxin domain of TXNRD1_v3 is an atypical regulator of the cell cytoskeleton that potently induces formation of highly ordered cytoplasmic filaments and cell membrane filopodia. (houstonmethodist.org)
Scholars@Duke publication: Phospholipid distribution in the cytoplasmic membrane of Gram-negative bacteria is highly asymmetric, dynamic, and cell shape-dependent. (duke.edu)
DiA 4-(4-Dihexadecylaminostyryl)-N-methylpyridinium iodide) is a green fluorescent membrane dye which diffuses much faster than DiO in cell membranes. (biotium.com)
1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C 18 carbon) hydrocarbon chains into the lipid bilayer. (biotium.com)
In Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc. (cdc.gov)
The flagellum is an ion-powered rotary motor, anchored in the membranes surrounding the bacterial cell. (biblestudymanuals.net)
Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
Dyes3
CellBrite™ Fix dyes are fluorogenic membrane dyes that covalently stain the plasma membrane in live cells. (biotium.com)
We also offer classic membrane and membrane potential dyes, including CellBrite™ and CellBrite™ NIR dyes , which are lipophilic carbocyanine dyes that stain the plasma membrane of cells and are non-toxic for long-term tracking. (biotium.com)
N-phenyl-1-naphthylamine and propidium iodide dyes were employed to determine membrane permeabilization. (bvsalud.org)
Vesicles1
Free-living' ICM vesicles, which possess all the machinery for converting light energy into ATP, can be regarded as bacterial membrane organelles. (nih.gov)
Plasma4
By providing an efflux pathway for base, anion exchangers constitute a key component of the plasma membrane proton-loading machinery. (bgu.ac.il)
Is extrinsic component of plasma membrane. (nih.gov)
Deficiency in plasma membrane calcium ATPase isoform 2 increases susceptibility to noise -induced hearing loss in mice. (cdc.gov)
Mice homozygous for a null mutation in the plasma membrane Ca2+-ATPase isoform 2 (PMCA2) gene are deaf (Kozel et al. (cdc.gov)
Leaflet1
As the total PE content increases from nearly zero to 75%, cells progressively adopt a rod shape and PE appears in the cytoplasmic leaflet of the IM. (duke.edu)
Cellular1
However, the mechanism-of-action of AMPs has not been fully characterized at the cellular level due to a lack of high-resolution imaging technologies that can capture cellular-membrane disruption events in the hydrated state. (bvsalud.org)
Tail1
Tyrosine phosphorylation as a conformational switch: a case study of integrin Beta3 cytoplasmic tail. (expasy.org)
Flagellar1
Flagellar assembly begins with structures in the cytoplasmic membrane and proceeds through steps that add the exterior structures in a proximal-to-distal sequence ( Fig. 1 ) 1 . (nature.com)
Electron1
In order to study the biogenesis of ICM in this bacterium mature (ICM) and precursor (upper pigmented band - UPB) membranes were purified and compared at the single membrane level using electron, atomic force and fluorescence microscopy, revealing fundamental differences in their morphology, protein organization and function. (nih.gov)
Strain1
In this strain, the cytoplasmic level of FlgM remained nearly constant, whereas FlgM secretion was again prevented by 10 μM or more CCCP ( Fig. 2c ). (nature.com)
Reveal2
Our high-resolution cryo-ET images reveal how pepD2M disrupts the E. coli membrane using a carpet/detergent-like mechanism. (bvsalud.org)
Our studies reveal the direct membrane-disrupting consequence of AMPs on the bacterial membrane by cryo-ET, and this information provides critical insights into the mechanisms of this class of antimicrobial agents. (bvsalud.org)
Structures1
Bacterial flagella contain a specialized secretion apparatus that functions to deliver the protein subunits that form the filament and other structures to outside the membrane 1 . (nature.com)
Surface1
The purple phototrophic bacteria synthesize an extensive system of intracytoplasmic membranes (ICM) in order to increase the surface area for absorbing and utilizing solar energy. (nih.gov)
Domain1
Using truncated versions of the protein we found that both the cytoplasmic filaments and the filopodia formation were exclusively dependent on the glutaredoxin domain of the protein. (houstonmethodist.org)
and (5) internalized membrane protein from both live and apoptotic cells was found in early endosomes, late endosomes, and lysosomes. (pitt.edu)
Formation1
Interestingly, actin polymerization was required for filopodia formation triggered by TXNRD1_v3, but not for generation of cytoplasmic filaments. (houstonmethodist.org)