• The term stem cell can be defined by two very important qualities: the cell has the ability to self-renew and, in a more general sense, the cell has not completed differentiation into its final state. (thefutureofthings.com)
  • This general definition includes a wide variety of cells with varying degrees of differentiation potential. (thefutureofthings.com)
  • Methods: To investigate this unexplained Th17-biased differentiation, we focused on understanding the immunobiology of B27-rat naive CD4+ T cells (Tn). (bvsalud.org)
  • As a consequence, cell fate acquisition and subsequent differentiation of endosperm tissues are affected to varying degrees of severity. (biologists.com)
  • Additionally, lingering expression or re-expression of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. (addgene.org)
  • Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
  • RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
  • These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
  • Cell lineage, or the study of the developmental differentiation of a blastomere, involves tracing a particular cell (blastomere) forward from its position in one of the three germ layers. (asu.edu)
  • Overexpression of FLI1 in erythroblasts causes inhibition of differentiation and ultimately the development of pre-T cell lymphoblastic leukemia/lymphoma [ 11 ]. (biomedcentral.com)
  • Cloning, expression, and differentiation-dependent regulation of SMAF1 in adipogenesis. (nih.gov)
  • SM-AP1-3 cells were immunopositive for keratin only, indicating their duct-epithelial or squamous cell differentiation, while SM-AP4/5 cells were positive for both keratin and S-100 protein, indicating their myoepithelial cell differentiation. (biomedcentral.com)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
  • These malignancies are further characterized by the maturity and differentiation of the individual cell types and are divided into acute leukemias such as acute myeloid leukemia and acute lymphoblastic leukemia and chronic leukemias such as chronic myeloid leukemia and chronic lymphocytic leukemia. (medscape.com)
  • Different characteristics of the nuclei and cytoplasm of the cell allow differentiation by instrumentation and microscopy. (medscape.com)
  • Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
  • Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
  • Since the Notch signaling pathway molecules play an important role in differentiation of epithelial cells, it is important to identify the presence of notch signaling molecules in SHED during the process of cell differentiation. (bvsalud.org)
  • The Notch signaling pathway provides important intercellular signaling mechanisms essential for cell fate specification and it regulates differentiation and proliferation of stem or progenitor cells by para-inducing effects 3-4 . (bvsalud.org)
  • Notch signaling pathway is also involved in the regulation of epithelial cell differentiation in various tissues 5-6 . (bvsalud.org)
  • A centromere , the point on a chromosome that attaches to the spindle fibers with a kinetochore during cell division, attaches the sister chromatids. (coursehero.com)
  • Mitosis includes prophase, prometaphase, metaphase, and anaphase, as well as telophase, during which chromosome copies are carefully separated in preparation for cytokinesis, where the cytoplasm divides. (coursehero.com)
  • In Biology this is the type of cell division that results in two daughter cells each with half the chromosome number of the parent cell. (emfacts.com)
  • Typically the nucleus contains only small groups of chromosome fragments. (emfacts.com)
  • First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)
  • When the oocyte is in the metaphase II stage of cell division, the spindle-chromosome complex is removed and inserted into a healthy donor oocyte from which the nucleus has already been removed. (msdmanuals.com)
  • Usually mutant cells are targeted for apoptosis (programmed cell death) but not always. (emfacts.com)
  • Increasing evidence indicates that EBV miRNAs target the host mRNAs involved in cell proliferation, apoptosis and transformation. (ijbs.com)
  • 2. Standard research tool to analyze a variety of molecular biology procedures, for example, cell senescence, cytokine induction, protein expression, apoptosis, and receptor-ligand interactions. (ubigene.us)
  • Loss of cell division cycle‑associated 5 promotes cell apoptosis by activating DNA damage response in clear cell renal cell carcinoma. (nih.gov)
  • Silencing oncogene cell division cycle associated 5 induces apoptosis and G1 phase arrest of non-small cell lung cancer cells via p53-p21 signaling pathway. (nih.gov)
  • Downregulation of CDCA5 Can Inhibit Cell Proliferation, Migration, and Invasion, and Induce Apoptosis of Prostate Cancer Cells. (nih.gov)
  • Apoptosis-inducing factor (AIF) translocation from themitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent celldeath. (2011globalhealth.org)
  • This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. (origene.com)
  • GIST cells were treated with IR700-12A8 and NIR light in vitro and vivo, and cell viability, histology and apoptosis were evaluated. (thno.org)
  • Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)
  • The artificial cloning of organisms, sometimes known as reproductive cloning, is often accomplished via somatic-cell nuclear transfer (SCNT), a cloning method in which a viable embryo is created from a somatic cell and an egg cell. (wikipedia.org)
  • The use of embryonic stem cells, which can be produced through SCNT, in some stem cell research has attracted controversy. (wikipedia.org)
  • Indeed, if passed, Hatch/Feinstein/Kerry would explicitly legalize doing in humans the very cloning procedure -- somatic cell nuclear transfer (SCNT) -- that was used to make Dolly the sheep . (lifeissues.net)
  • Comment: Indeed, if passed, "total cloning bans" H.R. 534, H.R. 234, H.R. 916, and S. 245 would not ban anything either - not even the SCNT cloning technique that was used to make Dolly the sheep. (lifeissues.net)
  • Twenty years have passed since Dolly the sheep was born by cloning (somatic cell nuclear transfer, SCNT) but the results of non-human mammalian cloning are very poor, and cause animal diseases and huge biological losses. (sibi.org)
  • I believe that the reprogramming errors are not the only cause of these low rates of cloning: the mammalian SCNT fails with a very high frequency mainly due to the damage that the technique itself inflicts in the egg and the somatic nucleus, and the very few successful cases occur only when the damage is not significant. (sibi.org)
  • True cloning performed by nuclear transfer from an adult and differentiated somatic cell to a previously enucleated egg (somatic cell nuclear transfer, SCNT), gives rise to a new cell, the nuclovulo (nucleus+ovum), distinct from the zygote because the sperm is not involved in its creation, while both can develop as embryos and give rise to offspring. (sibi.org)
  • Prior to SCNT, the somatic cell (differentiated) must be reprogramed to a similar state of a pluripotent embryonic cell (undifferentiated) before the nucleus is extracted and transferred. (sibi.org)
  • The second position is that of those who believe that the human zygote obtained by somatic cell nuclear transfer (SCNT) (cloning) is a different biological entity to the zygote obtained naturally (see our ethical assessment HERE ). (bioethicsobservatory.org)
  • Reproductive cloning is expensive and highly inefficient. (wikiquote.org)
  • and the general public debate about reproductive cloning. (edu.au)
  • Among the factors thought to contribute to the greater success in cloning cattle are the relatively late embryonic genome activation specific for this species [16 -18] and the optimization of reproductive technologies, such as in vitro embryo production and embryo transfer, brought about by the cattle industry [19]. (sibi.org)
  • Other cellular structures are duplicated during G2, such that each replicated daughter cell produced during mitosis will have all necessary organelles (such as mitochondria, endoplasmic reticulum, etc. (coursehero.com)
  • Аномалії мітохондріальної ДНК Each cell has several hundred mitochondria in its cytoplasm. (msdmanuals.com)
  • Temporal distribution studies identify hnRNPA1 and NP co-localize in the same cellular milieu in both nucleus and mitochondria in NP-transfected and IAV-infected mammalian cells. (cdc.gov)
  • Plasma cell myeloma (multiple myeloma, myelomatosis, medullary plasmacytoma) is a bone marrow-based, malignant disorder of postgerminal center B-cells that is characterized by a clonal proliferation of plasma cells, with associated serum and/or urine monoclonal proteins. (medscape.com)
  • It is still poorly understood how and which benign pleomorphic adenoma cells transform into its malignant form, carcinoma ex pleomorphic adenoma. (biomedcentral.com)
  • Although pathologists in their daily services of surgical pathology had recognized such singular atypical cells in pleomorphic adenomas, these atypical cells were not always regarded as evidence or the source for malignant transformation [ 4 - 6 ]. (biomedcentral.com)
  • blood transfusion), spinal cord, lung cancer (oncology), respiratory cancer, radiology Background Multiple myeloma is a malignant proliferation of plasma cells mostly affecting the bone marrow and commonly presents with anaemia, hypercalcaemia, renal dysfunction, bone and skeletal involvement. (2011globalhealth.org)
  • A group of heterogeneous lymphoid tumors generally expressing one or more B-cell antigens or representing malignant transformations of B-lymphocytes. (lookformedical.com)
  • Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. (lookformedical.com)
  • Malignant lymphoma composed of large B lymphoid cells whose nuclear size can exceed normal macrophage nuclei, or more than twice the size of a normal lymphocyte. (lookformedical.com)
  • Malignant lymphoma in which the lymphomatous cells are clustered into identifiable nodules within the LYMPH NODES. (lookformedical.com)
  • These are the malignant proliferations of abnormal clones of white blood cells within the bone marrow that are broadly categorized into lymphoid and myeloid neoplasms depending on the type of white cell proliferation. (medscape.com)
  • A dermatology work-up was initiated, and the síndrome de Sézary initial diagnosis of malignant T-cell neoplasm was confirmed. (bvsalud.org)
  • subtypes of cutaneous T-cell lymphoma, SS is a malignant neoplasm originating the most common of which are mycosis from T lymphocytes, which involves the fungoides and Sézary syndrome (SS). (bvsalud.org)
  • Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. (biomedcentral.com)
  • In eukaryotes, DNA is packed inside the cell nucleus in the form of chromatin, which consists of DNA, proteins such as histones, and RNA. (nature.com)
  • At the top of the list comes the zygote-a fertilized egg, which of course has the ability to divide and differentiate into all cell types in the body and create a new organism. (thefutureofthings.com)
  • The first three divisions of the zygote give birth to eight totipotent cells, each of which also has the ability to become an entire organism. (thefutureofthings.com)
  • The third position is that of those who consider that the single-cell, polarised, asymmetrical human embryo, the zygote, obtained naturally or artificially, is a living being of our species, bearer therefore of the dignity that all human beings intrinsically possess, and consequently worthy of being treated in accordance with that dignity. (bioethicsobservatory.org)
  • Then the male and female pronuclei are removed from the first zygote and transplanted into the donor zygote after its nucleus has been removed. (msdmanuals.com)
  • antibody (clone AD2.35) on mouse primary astrocytes. (biolegend.com)
  • The cells were fixed, permeabilized, blocked and then stained with primary antibody, and Alexa Fluor® 594 conjugated secondary IgG (Cat. (biolegend.com)
  • Human GIST cell lines GIST-T1 and GIST-882M were incubated with IR700-conjugated anti-c-KIT antibody, IR700-12A8, and observed by confocal laser microscopy. (thno.org)
  • However, a number of other features are needed, and a variety of specialised cloning vectors (small piece of DNA into which a foreign DNA fragment can be inserted) exist that allow protein production, affinity tagging, single-stranded RNA or DNA production and a host of other molecular biology tools. (wikipedia.org)
  • Retroviral vectors , such as pMXs or pMSCV, infect dividing cells, and have a higher efficiency of generating iPSCs compared to other delivery methods. (addgene.org)
  • Retrovirus has a cloning capacity of ~8 kb, so either a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming factors. (addgene.org)
  • Lentiviral vectors infect dividing and nondividing cells and have a larger cloning capacity (8-10 kb) than other retroviral vectors. (addgene.org)
  • Adult stem cells can be used to accelerate bone or tendon healing , and they can induce cartilage progenitor cells to produce a better matrix and repair cartilage damage . (thefutureofthings.com)
  • These proteins have been implicated in control of viral transcription and may possibly serve as the "glue" that binds the nucleocapsid to the appropriate membrane site during viral budding from the host cell. (lookformedical.com)
  • mega-dalton proteins lattice in the cytoplasm. (mdm-inhibitors.com)
  • Here, in order to discover those membrane proteins that may be involved in JEV attachment to or entry into virus permissive BHK-21 cells, a chemically mutated cell line (designated 3A10-3F) that became less susceptible to JEV infection was preliminarily established and selected by repeated low moi JEV challenges and RT-PCR detection for viral RNA E gene fragment. (biomedcentral.com)
  • Finally, two-dimensional electrophoresis (2-DE) coupled with LC-MS/MS was utilized to recognize the most differentially expressed proteins from membrane protein extracts of 3A10-3F and BHK-21 cells respectively. (biomedcentral.com)
  • The noted discrepancy of membrane proteins included calcium binding proteins (annexin A1, annexin A2), and voltage-dependent anion channels proteins (VDAC 1, VDAC 2), suggesting that these molecules may affect JEV attachment to and/or entry into BHK-21 cells and worthy of further investigation. (biomedcentral.com)
  • A recent paper also indicated that several proteins on the surface of C6/36 cells with masses ranging from 35-80 kDa and 150-200 kDa may bind to JEV, but failed to identify specific proteins by mass spectroscopic fingerprint analysis [ 11 ]. (biomedcentral.com)
  • Purified GST fusion proteins of the N and C termini of Na v 1.3 pull down contactin from lysates of transfected HEK 293 cells. (jneurosci.org)
  • METHODS: The expression of PD-L1 and STAT1, and the lysine acetylation of STAT1 in CRC cells were observed after treatment with butyrate (2, 5, and 10 mM) for 24h or butyrate (5 mM) for 8, 16, and 24h. (bvsalud.org)
  • CONCLUSION: Butyrate strengthens the immune response to CRC cells by suppressing PD-L1 expression via acetylation of STAT1. (bvsalud.org)
  • The objectives of this study were to assess the expression of CXorf61 in TNBCs and healthy tissues and to evaluate its capability to induce T cell responses. (oncotarget.com)
  • In CXorf61-negative breast cancer cell lines CXorf61 expression is activated by treatment with the hypomethylating agent 5-aza-2′-deoxycytidine. (oncotarget.com)
  • The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
  • In non-small cell lung cancer, the FLI1 expression score is associated with the stage of SCLC. (biomedcentral.com)
  • Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. (thermofisher.com)
  • Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. (thermofisher.com)
  • EBV miRNAs also inhibit the expression of viral antigens, thereby enabling infected cells to escape immune recognition. (ijbs.com)
  • Key molecular studies performed with A549 cell as anti-tumor drug permeability and efficacy analysis, infection assays, respiratory immunotoxicity tests, cell senescence studies, and cytokine expression profiling. (ubigene.us)
  • Plays a key role in increased proteasome activity in bryonic st cells (ESCs): its high expression in ESCs promotes enhanced assbly of the 26S proteasome, followed by higher proteasome activity. (joplink.net)
  • Molecular cloning and expression of subunit 9 of the 26S proteasome.Hoffman L., Rechsteiner M.FEBS Lett. (joplink.net)
  • Contactin/F3, a cell adhesion molecule, has been shown to interact with and enhance surface expression of sodium channels Na v 1.2 and Na v 1.9. (jneurosci.org)
  • The expression of contactin and Na v 1.3 in the HEK-Na v 1.3 stable cell line significantly increases the current density. (jneurosci.org)
  • Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
  • To examine its role in cells, our RNA-seq analysis showed that the expression of S970D causes decreased expression of MOV10 enhanced Cross-Linking Immunoprecipitation (eCLIP) targets compared to WT. (cdc.gov)
  • To better understand the cellular components controlling alphavirus vector specificity, a cell culture model system of the anopheline restriction of CHIKV was developed along with a protein expression strategy. (cdc.gov)
  • A cell spends most of its time in interphase, during which it mostly performs its normal functions, such as protein synthesis. (coursehero.com)
  • KIN17, which is known as a DNA and RNA binding protein, is highly expressed in numerous types of human cancers and was discovered to participate in several vital cell behaviors, including DNA replication, damage repair, regulation of cell cycle and RNA processing. (spandidos-publications.com)
  • Previous studies have indicated that KIN17 is involved in global genome repair, DNA replication, transcription and regulation of the cell cycle as part of a multi-protein complex. (spandidos-publications.com)
  • Rb , or retinoblastoma protein, is a key regulator of the cell cycle, particularly during the transition from the G1 to S phases. (biolegend.com)
  • Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)
  • Asymptomatic plasma cell myeloma is a plasma cell neoplasm defined by the presence of serum monoclonal protein of 3.0 gm/dL or greater (by electrophoresis) and/or clonal plasma cells in the bone marrow of 10% or greater, without evidence of end-organ damage (hypercalcemia, renal insufficiency, anemia, lytic lesions of bone). (medscape.com)
  • Symptomatic plasma cell myeloma is a plasma cell neoplasm defined as the presence of end-organ damage (hypercalcemia, renal insufficiency, anemia, lytic lesions of bone) in a patient with a monoclonal protein (of any amount) and clonal plasma cells in the bone marrow (of any amount) or plasmacytoma. (medscape.com)
  • Much earlier, a report stated that a 74 kDa protein on Vero cells was found to be capable of binding JEV and might be involved in virus uptake process [ 10 ]. (biomedcentral.com)
  • Full length human recombinant protein of human LGALS3(NP_002297) produced in HEK293T cell. (origene.com)
  • This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. (origene.com)
  • It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
  • So far the reprogramming of somatic cells shows very low rates of efficiency (~0.0006-1%) that have not improved in the last two decades of continuous research. (sibi.org)
  • 53% of TNBC patients express this antigen in at least 30% of their tumor cells. (oncotarget.com)
  • In addition, knockdown of KIN17 was found to enhance the sensitivity of tumor cells to chemotherapeutic agents. (spandidos-publications.com)
  • The above biological processes have important roles in tumorigenesis, cancer development and chemoresistance in tumor cells. (spandidos-publications.com)
  • In addition, they had ductal arrangements or plasmacytoid appearances of tumor cells and myxoid or hyaline stromata, indicating some characteristics of pleomorphic adenoma. (biomedcentral.com)
  • In addition to their ability to supply cells at the turnover rate of their respective tissues, they can be stimulated to repair injured tissue caused by liver damage, skin abrasions and blood loss. (thefutureofthings.com)
  • The ability of our body to regenerate some of its tissues is largely owed to the reserves of adult stem cells. (thefutureofthings.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Stem cells may be derived from adult tissues but the most potent are extracted from developing human embryos. (edu.au)
  • This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth. (lookformedical.com)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • Stem cells from human exfoliated deciduous teeth (SHED) are multipotent stem cells derived from the pulp tissues of extracted deciduous teeth 1 . (bvsalud.org)
  • Anemia results from bone marrow replacement by plasma cell myeloma and/or the loss of erythropoietin as a result of renal injury. (medscape.com)
  • Decreased WBC count, leukopenia, is seen when supply is depleted by infection or treatment such as chemotherapy or radiation therapy, or when a hematopoietic stem cell abnormality does not allow normal growth/maturation within the bone marrow, such as myelodysplastic syndrome or leukemia. (medscape.com)
  • A bone marrow smear was performed, in which 'cerebriform' cells were identified, confirming the diagnosis of Sézary syndrome. (bvsalud.org)
  • A fifth phase has been identified, G0, in which the cell is resting just after mitosis but still carrying out normal functions. (coursehero.com)
  • The M phase consists of mitosis, in which the cell divides, producing two new, identical cells. (coursehero.com)
  • Interphase is collectively the gap 1 (G1), synthesis (S), and gap 2 (G2) phases of mitosis, in which a cell grows, replicates its DNA, and grows again. (coursehero.com)
  • Here we combine theory and experiment to analyze the dynamics of euchromatin organization as pluripotent zebrafish cells exit mitosis and begin transcription. (nature.com)
  • The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1). (lookformedical.com)
  • Ultrastructurally, neoplastic cells were characterized by abundant microvilli at their apical pole and by numerous junctional complexes on lateral cell membranes. (duckdvm.com)
  • Plasma cell lesions are the neoplastic (clonal) proliferation of plasma cells involving single bones (plasmacytoma) or multiple bones (multiple myeloma). (medscape.com)
  • Cells grown in vitro from neoplastic tissue. (lookformedical.com)
  • Neoplastic proliferations of white blood cells also cause leukocytosis. (medscape.com)
  • More than 100 nuclear transfer procedures could be required to produce one viable clone. (wikiquote.org)
  • About 50% of the proliferating epithelial cells showed positive nuclear labeling for estrogen and progesterone receptors, and approximately one-third were positive for Ki-67. (scielo.br)
  • In addition, the myoepithelial cells exhibited diffuse nuclear immunoreactivity for p63. (scielo.br)
  • Cloning and characterization of T cell receptors (TCRs) from responding T cells resulted in the identification of the two HLA-A*0201-restricted T cell epitopes CXorf61 66-74 and CXorf61 79-87 . (oncotarget.com)
  • Apolipoprotein B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation. (thermofisher.com)
  • The pathogenesis of Japanese encephalitis virus (JEV) is not definitely elucidated as the initial interaction between virus and host cell receptors required for JEV infection is not clearly defined yet. (biomedcentral.com)
  • Occasionally, the term cloning is misleadingly used to refer to the identification of the chromosomal location of a gene associated with a particular phenotype of interest, such as in positional cloning. (wikipedia.org)
  • A causative gene for this disease, NPHS2, was mapped to 1q25-31 and we report here its identification by positional cloning. (scienceopen.com)
  • HN - 2008 BX - Von Ebner's Glands MH - Cumulus Cells UI - D054885 MN - A05.360.319.114.630.535.200.500 MN - A06.407.312.497.535.300.500 MN - A11.436.300.500 MS - The granulosa cells of the cumulus oophorus which surround the OVUM in the GRAAFIAN FOLLICLE. (bvsalud.org)
  • The early mammalian embryo consists of the extra-embryonic cell layers-the trophoblast and a body of cells called the inner cell mass (ICM), which eventually become the embryo proper. (thefutureofthings.com)
  • Here we report that human hnRNPA1 physically interacts with the nucleoprotein (NP) of IAV in mammalian cells at different time points of the viral replication cycle. (cdc.gov)
  • Natural cloning is the production of clones without the involvement of genetic engineering techniques. (wikipedia.org)
  • The main difference between the two is that natural cloning does not involve any human intervention, whereas artificial cloning is a genetic engineering technique. (wikipedia.org)
  • Interphase comprises the gap 1 (G1), synthesis (S), and gap 2 (G2) phases, in which the cell grows and replicates its genetic material. (coursehero.com)
  • However the aim of cloning in research is to have genetic uniformity. (emfacts.com)
  • However, A549 cells harbor oncogenic KRASG12S mutation as well as over 250 genetic mutations (COSMIC cell lines project), including some known oncogenes and tumor suppressors. (ubigene.us)
  • what would remain within a cell membrane, if you removed the a) nucleus, which contains the DNA and chromasomes and b) the mitochondrial DNA. (emfacts.com)
  • Strong red fluorescence of IR700-12A8 was observed on the cell membrane of GIST cells and was gradually internalized into the cytoplasm. (thno.org)
  • We propose the outline of such a mechanism based on following aspects: (1) The glomerulus is a complex structure, the stability of which depends on the cooperative function of the basement membrane, mesangial cells and podocytes, counteracting the distending forces originating from the high glomerular hydrostatic pressures. (scienceopen.com)
  • Both have a cell membrane, kind of like the nightclub door that allows substances to enter and exit. (opensimworld.com)
  • Another example of artificial cloning is molecular cloning, a technique in molecular biology in which a single living cell is used to clone a large population of cells that contain identical DNA molecules. (wikipedia.org)
  • In biology , cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria , insects or plants reproduce asexually . (wikiquote.org)
  • To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
  • A549 cells are used in a variety of basic cell biology and biomedical studies involving lung cancer proliferation, corresponding inhibitors studies, tumor formation, and metastases. (ubigene.us)
  • Plasma cell myeloma presents with generalized marrow involvement, with or without lytic lesions of bone. (medscape.com)
  • Molecular cloning refers to the process of making multiple molecules. (wikipedia.org)
  • Cloning in biotechnology refers to processes used to create copies of DNA fragments ( molecular cloning ), cells (cell cloning), or organisms . (wikiquote.org)
  • This early molecular phenomenon might lead to the persistent proinflammatory skew of CD4+ T cells in SpA patients, thus offering new clues to better understand and treat SpA. (bvsalud.org)
  • Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response. (lookformedical.com)
  • These cells also utilized to study a variety of molecular characteristics for human tumors in culture. (ubigene.us)
  • When investigating the causes of this failure via cellular and molecular analysis of 2-cell zygotes and the successive cell divisions (blastomeres), all kinds of abnormalities were found. (sibi.org)
  • Cloning is the process of producing individual organisms with identical genomes, either by natural or artificial means. (wikipedia.org)
  • In the field of biotechnology, cloning is the process of creating cloned organisms of cells and of DNA fragments. (wikipedia.org)
  • Natural cloning occurs through a variety of natural mechanisms, from single-celled organisms to complex multicellular organisms. (wikipedia.org)
  • On the other hand, a chimera is defined as an organism in which cells from two or more different organisms have contributed. (frontiersin.org)
  • Driesch showed that the cells of an early embryo, when separated, could each continue to develop into normal larval forms. (asu.edu)
  • The first position is that of those who consider that the human embryo, in its first days of life, is a cell cluster with no biological structure, i.e. an unorganised cluster of cells and, accordingly, with no biological or ontological value. (bioethicsobservatory.org)
  • In other words, this law accepts the obsolete theory that identifies the human embryo as a cluster of cells. (bioethicsobservatory.org)
  • RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). (bvsalud.org)
  • While regarded by many top scientists as the Holy Grail of medicine, others consider embryonic stem-cell research sacrilegious. (thefutureofthings.com)
  • Experts from around the world are assessing the difficult issue of the extent to which embryonic stem cell research should be allowed to proceed, and to date there is little international consensus on this matter. (edu.au)
  • How, then, should embryonic stem cell research be regulated in Australia? (edu.au)
  • In this article we examine embryonic stem cell research and explore the current regulatory framework associated with this research in Australia, with particular reference to the Andrews Report . (edu.au)
  • Recent advances in the field of stem-cell research are giving hope to millions. (thefutureofthings.com)
  • Avant-garde approaches to stem-cell therapy may be the first stepping-stones to a bright new future of stem-cell medicine and are emerging in leading laboratories worldwide. (thefutureofthings.com)
  • A particular field encouraged by the foundation is stem-cell research, with the great hope that it will result in the ability to get cells to differentiate into neurons and support cells to bridge the gap of a spinal cord injury. (thefutureofthings.com)
  • It's given name is the "Human Cloning Ban and Stem Cell Research Protection Act of 2003," the stated purpose of which, supposedly, is to "prohibit human cloning and to protect important areas of medical research, including stem cell research. (lifeissues.net)
  • The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and wait for cells to de-differentiate. (addgene.org)
  • Stem cell technology is the latest development in this controversial branch of science. (edu.au)
  • Embryonic stem cell technology is still at a preliminary research stage and announcements about its potential may be premature. (edu.au)
  • The ethical and legal controversies that were aroused in the ART debates during the 1980s have been re-ignited with the development of stem cell technology. (edu.au)
  • This issue was considered by the House of Representatives Standing Committee on Legal and Constitutional Affairs in its report entitled Human Cloning: Scientific, Ethical and Regulatory Aspects of Human Cloning and Stem Cell Research (hereafter the Andrews Report , after the Chair of the Committee, Mr Kevin Andrews, MP) released in September 2001. (edu.au)
  • Stem cell technology in humans derives from earlier and complementary work in animal studies. (edu.au)
  • The patient went on to have chemotherapy and autologous stem cell transplantation with a good outcome. (2011globalhealth.org)
  • B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma . (lookformedical.com)
  • Lymphoma b cell. (lookformedical.com)
  • A form of non-Hodgkin lymphoma having a usually diffuse pattern with both small and medium lymphocytes and small cleaved cells. (lookformedical.com)
  • Animal models for human tumour mental animals is not easy to answer does induce adult T-cell leukaemia/ viruses that make use of animal virus- for these agents, because cancer bi- lymphoma (ATLL), albeit in monkeys es are scarce. (who.int)
  • Cutaneous T-cell lymphoma is a general term from mycosis fungoides or occur spontane- to identify non-Hodgkin's T-cell lymphomas ously, although some authors now consider that primarily affect the skin. (bvsalud.org)
  • Furthermore, KIN17 is associated with cancer cell proliferation, migration, invasion and cell cycle regulation by regulating pathways including the p38 MAPK, NF‑κB‑Snail and TGF‑β/Smad2 signaling pathways. (spandidos-publications.com)
  • Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases. (ubigene.us)
  • The unique properties of human stem cells have aroused considerable optimism about their potential as new pathways for alleviating human suffering caused by disease and injury. (edu.au)
  • The effect of butyrate on the cytotoxicity of CD8+ T-cells against CRC cells with or without PD-L1 overexpression was explored in vitro and in vivo. (bvsalud.org)
  • Furthermore, by in vitro priming of human CD8+ T cells derived from a healthy donor recognizing CXorf61 66-74 we were able to induce a strong antigen-specific immune response and clone a human TCR recognizing this epitope. (oncotarget.com)
  • This study demonstrates in vitro that certain cell types from pleomorphic adenoma are able to clone and survive over a long term and develop subcutaneous tumors in nude mice. (biomedcentral.com)
  • Developments in biotechnology have raised new concerns about animal welfare, as farm animals now have their genomes modified (genetically engineered) or copied (cloned) to propagate certain traits useful to agribusiness, such as meat yield or feed conversion. (wikiquote.org)
  • Cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non-coding sequences and randomly fragmented DNA. (wikipedia.org)
  • The chromasomes have divided to form new pair of cells, but the fragments of the DNA have rejoined in an incorrect order, resulting in a change in the operations of the cell. (emfacts.com)
  • Cellular therapy based on mesenchymal stem cells (MSCs) is a promising novel therapeutic strategy for the osteonecrosis of the femoral head (ONFH), which is gradually becoming popular, particularly for early-stage ONFH. (biomedcentral.com)
  • In either form of plasma cell myeloma, immune dysfunction, cytopenias and lytic bone lesions occur as a result of direct cellular interactions (cytokines, growth factors) and/or mass effect. (medscape.com)
  • Such broad tropism of JEV rationally suggests that most possibly there exists more than one cellular receptor responsible for virus binding and entry into susceptible cells above mentioned. (biomedcentral.com)
  • The efficiency of cloning, defined as the proportion of transferred embryos that result in viable offspring, is approximately 2 to 3% for all species. (sibi.org)
  • Finally, this threat also extends to embryos produced by cloning and parthenogenesis , which can then be used for presumably therapeutic and, in particular, experimental ends, mainly to obtain embryonic cell lines that can then be used for biomedical experiments, leading to the inevitable destruction of the embryos created. (bioethicsobservatory.org)
  • The process by which the body regulates the erratic growth of cells by insructing certain cells to die. (emfacts.com)
  • RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)
  • RESULTS: Butyrate could suppress IFN-γ-induced PD-L1 up-regulation in CRC cells in a dose- and time-dependent way. (bvsalud.org)
  • and regulation of cell cycle process. (nih.gov)
  • These cells are considered pluripotent . (thefutureofthings.com)
  • The field of induced pluripotent stem cells (iPSCs) has been around for 10 years. (addgene.org)
  • As an alternative approach, MSCs derived from induced pluripotent stem cells (iPSCs), which may lead to further standardized-cell preparations. (biomedcentral.com)
  • Here, we combine experiments in pluripotent zebrafish cells and theory to show that euchromatin is organized in line with an active microemulsion model. (nature.com)
  • Based on our findings, however, we propose that specification of BETL cells is an irreversible event that occurs within a narrow window of syncytial development, and that BETL cell identity is subsequently inherited in a lineage-dependent manner. (biologists.com)
  • [ 1 ] Extraosseous solitary plasmacytoma of bone is a localized, soft-tissue plasma cell neoplasm that occurs in a location other than the bone and does not have evidence of plasma cell myeloma or marrow plasmacytosis. (medscape.com)
  • [ 6 ] In addition, plasma cell myeloma occurs more commonly in Americans of African descent than of European descent. (medscape.com)
  • One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). (lookformedical.com)
  • Claims that you could clone individual treatments of human beings to treat common diseases like diabetes, suggests you need a huge supply of human eggs. (wikiquote.org)
  • The report arose out of a recommendation for the Committee to review the report of the Australian Health Ethics Committee (AHEC) of the NHMRC entitled Scientific, Ethical and Regulatory Considerations Relevant to Cloning of Human Beings (hereafter the AHEC Report ). (edu.au)
  • Renal cell carcinoma (RCC) incidence has increased over the past two decades. (hindawi.com)
  • Renal cell carcinoma (RCC) accounts for approximately 3% of human malignancies, and its incidence appears to be increasing globally [ 1 ]. (hindawi.com)
  • At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide. (ubigene.us)
  • One of the most convincing methods to verify a putative virus receptor is to transfer the receptor gene into a cell line that cannot bind virus and later demonstrate that the receptor-negative cell acquires the ability to bind virus and permit virus to replicate within it after the receptor gene is regained [ 13 ]. (biomedcentral.com)
  • However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
  • Nonetheless, the MSC-based therapy is challenging due to certain limitations, such as limited self-renewal capability of cells, availability of donor MSCs, and the costs involved in donor screening. (biomedcentral.com)
  • It is likely that polyclonal B-cell lymphoproliferation in AIDS is a complex result of EBV infection, HIV antigenic stimulation, and T-cell-dependent HIV activation. (lookformedical.com)
  • Established cell cultures that have the potential to propagate indefinitely. (lookformedical.com)
  • In rodents, and even in some preliminary trials in humans, human embryonic stem cells have been shown to bridge gaps in spinal cord injuries , allowing restoration of motor functions. (thefutureofthings.com)
  • The type species of LYMPHOCRYPTOVIRUS, subfamily GAMMAHERPESVIRINAE , infecting B-cells in humans. (lookformedical.com)
  • One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
  • that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
  • however, there are also stem cells in the adult body. (thefutureofthings.com)
  • These adult stem cells are considered multipotent, having the ability to differentiate into different cell types, albeit with a more limited repertoire than embryonic stem cells. (thefutureofthings.com)
  • Adult stem cells can be used to replace damaged heart-muscle cells and are used in practice today . (thefutureofthings.com)
  • The most common application of adult stem cells is probably the restoration of blood cells for patients with leukemia, and there are many more applications currently in practice. (thefutureofthings.com)
  • Stem cells are unique due to their ability to limitlessly self-renew and differentiate into each cell type in the adult body. (biolegend.com)
  • If artificial cloning and natural cloning both lead to the same result, which is the formation of a clone, that is, an organism with identical or nearly identical genes to another organism, then the plight of This creation is very different between the two creatures. (wikipedia.org)
  • Human papillomavirus DNA detection in head and neck squamous cell carcinoma has been linked to improved patient prognosis. (biomedcentral.com)
  • Transplanted cells in nude mice formed subcutaneous tumors, which had histological features of squamous cell carcinoma with apparent keratinizing tendencies. (biomedcentral.com)
  • The histological features of squamous cell carcinoma from the transplanted cell systems in nude mice might suggest a secondary onset of malignancy from a pre-existing benign adenoma. (biomedcentral.com)
  • Potential features that attract the investigators are epithelial cells with squamous nature and responsible for the diffusion of substances, such as water and electrolytes, across the alveoli of the lungs. (ubigene.us)
  • A549 cells used as a type II pulmonary epithelial cell model for drug metabolism and as a transfection host. (ubigene.us)
  • The ICM continues to differentiate into three germ layers-ectoderm, mesoderm and endoderm, each of which follows a specific developmental destiny that takes them along an ever-specifying path at which end the daughter cells will make up the different organs of the human body. (thefutureofthings.com)
  • Labeling individual cells within their germ layers allows for a pictorial interpretation of gastrulation. (asu.edu)
  • is the term applied to laboratory techniques using cell-cultures (tissue culture), as distinct from those using human subjects or live animals for testing possible harmful products. (emfacts.com)
  • Polyinosine-polycytidylic acid (pIC) is a synthetic double-stranded RNA (dsRNA) which directly activates dendritic cells and natural killer cells inhibiting tumor growth. (bvsalud.org)
  • RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)