Cytoplasm cell fractionation. Medical search. Frequent questions

Here we used subcellular fractionation and immunofluorescence to understand such complex functions of UPF1. (molcells.org)
However, in an investigation of BRAF localisation using fluorescence microscopy combined with subcellular fractionation of Green Fluorescent Protein (GFP)-tagged proteins expressed in NIH3T3 cells, surprisingly, we detected N-terminally truncated BRAF (ΔBRAF) in both nuclear and cytoplasmic compartments. (bath.ac.uk)
The subcellular location of DNAJC3-AS1 was explored with cellular subcellular fractionation assay. (mjhid.org)
Nucleo-cytoplasmic shuttling of HuR was detected by subcellular fractionation and confocal microscopy in MK2 knockdown HPMECs. (biomedcentral.com)
Subcellular fractionation further indicated that GAPDH remained relatively constant in the cytosol, while increasing at 12 to 24 hours postinfection (hpi) and decreasing at 36 hpi in the nuclear fraction of infected cells. (biomedcentral.com)

cIAP1 is a multi-functional protein which can be found in the cytoplasm of cells and in the nucleus of tumor cells. (wikipedia.org)

Moreover, it functions in nonsense-mediated decay (NMD) and therefore commutes between the nucleus and the cytoplasm. (uni-goettingen.de)
Typically it shuttles between the nucleus and the cytoplasm to perform its functions, while abnormal cytoplasmic aggregation of TDP-43 has been associated with neurodegenerative diseases amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). (nature.com)

We recently developed a microfluidic system that enables the high-throughput sequencing of cytoplasmic and nuclear RNA with the physical fractionation of the RNA species via electrical lysis and isotachophoresis. (riken.jp)
The clones expressing the highest levels of transcript, were analyzed by Immunoﬂuorescence imaging and Nuclear/cytoplasmic fractionation in order to evaluate hTERT subcellular localization. (123dok.org)
Generally, these receptors explain their function on the cell membrane where, after the binding with growth factors or neuregulin undergo to homo or hetero oligomerization with the activation of the intrinsic tyrosine kinase activity and the subsequent recruitment of proteins involved in the cytoplasmic signalling pathways. (biomedcentral.com)
Here, we combined long-read sequencing with metabolic labeling, splicing inhibition and cell fractionation experiments to quantify, separately, the genesis and trimming of nuclear and cytoplasmic tails in vitro and in vivo . (humantechnopole.it)
Biochemical fractionation showed that IglA is a soluble cytoplasmic protein and immunoprecipitation experiments demonstrate that it interacts with the downstream-encoded IglB. (biomedcentral.com)

MAPKs are involved in directing cellular responses to a diverse array os stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines regulating cell functions including proliferation, gene expression, differentiation, mitosis, cell survival or apoptosis. (wikipedia.org)
Combined treatment with selumetinib and a dose of palbociclib sufficient to reinforce G1 arrest in selumetinib-sensitive cells, but not to impair proliferation of resistant cells, delays the emergence of resistant colonies, meaning that escape from G1 arrest is critical in the formation of resistant clones. (babraham.ac.uk)
Functionally, JPX promoted lung cancer cell proliferation in vitro and facilitated lung tumor growth in vivo. (biomedcentral.com)
Instead ErbB3 silencing or nucleolar stress reduce cell proliferation and affect cell cycle progression. (biomedcentral.com)
These data point to the existence of an ErbB3-mediated non canonical pathway that glioblastoma cells use to control ribosomes synthesis and cell proliferation. (biomedcentral.com)
Nucleolar activity is also influenced by the interaction between pathways activated from extracellular signals in order to coordinate ribosome synthesis and cell proliferation [ 6 ]. (biomedcentral.com)
Cell proliferation analysis was done with BrdU assay. (mjhid.org)
DNAJC3-AS1 increased cell proliferation and inhibited the role of miR-140 in suppressing cell proliferation. (mjhid.org)
Conclusion：In conclusion, miR-140 may target DNAJC3-AS1 to suppress cell proliferation in AML. (mjhid.org)
CircHERC1 was significantly upregulated in lung cancer tissues and cells, ectopic expression of circHERC1 strikingly facilitated the proliferation, invasion and metastasis, and inhibited the apoptosis of lung cancer cells in vitro and in vivo. (biomedcentral.com)
The accumulation of FOXO1 in the cytosol and nuclear exclusion promoted cell proliferation and inhibited apoptosis. (biomedcentral.com)
In addition, circHERC1 can promote cell proliferation and inhibit apoptosis by sequestering FOXO1 in the cytoplasm to regulate AKT activity and BIM transcription. (biomedcentral.com)
Cell proliferation and apoptosis were measured using CCK8, EdU staining, flow cytometry, and TUNEL assays. (biomedcentral.com)
Number 2 Subcellular distribution of the TIA1 isoforms Involvement of TIA1 in ESCC cell proliferation To gain insight into the potential function Lidocaine (Alphacaine) of TIA1 whose overexpression could be associated with esophageal carcinogenesis we 1st tested the effects of small interfering RNA (siRNA) focusing on TIA1 on cell proliferation. (mdm-inhibitors.com)
By silencing endogenous TIA1 using three different siRNAs cell proliferation was significantly suppressed in KYSE140 KYSE180 and TE4 cells. (mdm-inhibitors.com)
Number 3 Effects of TIA1 knockdown on cell proliferation We next launched a TIA1[v1]- or TIA1[v2]-expressing retrovirus into KYSE190 and KYSE2270 cells expressing relatively lower levels of endogenous TIA1 to determine the effects of exogenously indicated TIA1 isoforms. (mdm-inhibitors.com)
Cell proliferation was tested by MTT assay. (spandidos-publications.com)
It is reportedly involved in many physiological functions of cells, including apoptosis, proliferation, invasion and angiogenesis ( 5 ). (spandidos-publications.com)
Each category of histiocytosis can be traced to reactive or neoplastic proliferation in one of these cell lineages. (medscape.com)

When a cell is tumorous it does not cease to proliferate inhibiting the apoptosis, as a result, in cancerous cells cIAP1 is rarely located in the cytoplasm. (wikipedia.org)
cIAP1 is recruited to TNF receptor complexes where they support cell survival through NF-κB activation while suppressing apoptosis by preventing caspase activation. (wikipedia.org)
Studies show that when the protein is exposed to palmitate the concentration of cIAP1 decreases and, as a result, the apoptosis is no longer inhibited resulting in the death of the cell. (wikipedia.org)
Mutations on the gene that encodes cIAP1 are related to hemorrhage and vascular regression because of the defects it represents on the endothelial cell survival and the modification of apoptosis. (wikipedia.org)
The impact of JPX on Twist1 expression, cell growth, invasion, apoptosis, and in vivo tumor growth were investigated in lung cancer cells by western blotting, rescue experiments, colony formation assay, flow cytometry, and xenograft animal experiment. (biomedcentral.com)
Studies have shown that endothelial cells (ECs) are the direct targets of hypoxia and are involved in the pathogenesis process of HPH leading to cell hyperproliferation, inhibition of apoptosis and plexiform intima injuries (Ranchoux et al. (biomedcentral.com)
Taken together, these outcomes indicate that endogenous TGFB signaling is respon sible for a substantial degree of apoptotic signaling in FET cells as abrogation of TGFB inhibitory signaling within the FET DN cells rendered the cells much more resistant to apoptosis. (sirnalibrary.com)
These results suggest that TIA1 silencing in ESCC cells contributes to cell cycle arrest in the G1-S checkpoint and the induction of apoptosis. (mdm-inhibitors.com)

AF cells (AFCs) represent a heterogeneous population composed of three groups of adherent cells, categorized based on their morphological, proliferative and biochemical characteristics: epithelioid (E-type), amniotic ﬂuid (AF-type) and ﬁbroblastic (F-type) cells[2]. (123dok.org)
Remarkably, biochemical fractionation exposed that a majority of METTL16 protein resides in the cytoplasm of a number of cells. (diferencias-entre.org)
Furthermore, the biochemical pathways and mechanisms that determine isotopic fractionation during biosilicification remain poorly understood. (copernicus.org)
Our results highlight that there is a taxonomic component to silicon isotope fractionation during biosilicification, possibly via a shared or related biochemical transport pathway. (copernicus.org)

A number of polypeptides synthesized specifically in Trichoplusia ni multiple nucleocapsid nuclear polyhedrosis virus ( T. ni MNPV)-infected Spodoptera frugiperda cells are phosphorylated both early and late in infection. (microbiologyresearch.org)
Infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus. (microbiologyresearch.org)
Protein synthesis in cells infected by Autographa californica nuclear polyhedrosis virus (Ac-NPV): the effect of cytosine arabinoside. (microbiologyresearch.org)
Two tissue culture media for production of lepidopteran cell lines and nuclear polyhedrosis viruses. (microbiologyresearch.org)
Baculovirus replication: protein synthesis in Spodoptera frugiperda cells infected with Trichoplusia ni nuclear polyhedrosis virus. (microbiologyresearch.org)
However, when the cell is apoptotic nuclear export of cIAP1 is induced provoking an increase in the cytosolic concentration of the protein. (wikipedia.org)
Animal steroid hormones initiate signaling by passive diffusion into cells and binding to their nuclear receptors to regulate gene expression. (biologists.com)
Most strikingly, in comparison to the nuclear retention of faulty mRNAs in the exosome mutant rrp6Δ and the CPF-CF complex mutant cft2-1, these RNAs leak into the cytoplasm when functional Hrp1 is missing. (uni-goettingen.de)
DNAJC3-AS1 was detected in both nuclear and cytoplasm samples, and a direct interaction between DNAJC3-AS1 and miR-140 was observed. (mjhid.org)
As proven by detection of nuclear Smad proteins, the TGFB and BMP signaling pathways are lively in many cells of the growth plate and they are controlled by tight temporal and regional patterns of expression of your components in the TGFB superfamily and of their receptors. (sirnalibrary.com)

In eukaryotic cells, various proteins and nucleic acids are orderly arranged in the nucleus and cytoplasm ( Handwerger and Gall, 2006 ). (aspetjournals.org)

In MEF cells with wild type Nrf2 and mouse hepa 1c1c7 cells, Cd increased the Nrf2 protein level but not the Nrf2 mRNA level dose-dependently. (cdc.gov)
Certain non-structural proteins and the major basic internal protein are the main phosphoproteins detected in infected cells. (microbiologyresearch.org)
In healthy cells the protein is usually found in the nucleus. (wikipedia.org)
The era of immune checkpoint blockers (ICB), which started in 2011 with the approval of an antibody targeting cytotoxic T lymphocyte-associated protein 4 (CTLA-4) on T cells (namely, ipilimumab) for the treatment of metastatic melanoma ( 1 ), has brought a new paradigm to cancer therapy whereby the immune system is being harnessed to cure the cancer. (aacrjournals.org)
Conclusions: Our study demonstrates for theﬁrst time the expression of the full protein hTERT by AFCs and its release outside the cell mediated by EVs, indicating a new extra telomeric role for this protein. (123dok.org)
Thus, the control of ribosome assembly and protein synthesis is essential for the survival of cancer cells. (biomedcentral.com)
In this context, during any sort of stress condition the alternative reading frame protein p14ARF subtracts MDM2, an E3 ubiquitin ligase, from the interaction with p53 in manner to free and stabilise p53 level in the nucleolus of the cell where it inhibits RNA polymerase I activity [ 3 ]. (biomedcentral.com)
2 Protein interactions are critical to the stability, localization and activity of b-catenin, and we recently performed the first proteomic analyses of the b-catenin interactome in myeloid cells. (haematologica.org)
3 This study identified Wilms tumor protein (WT1) as a putative novel interaction partner in myeloid cells. (haematologica.org)
In order to idenitfy appropriate cell lines in which to study b-catenin:WT1 interplay we first performed a screen of myeloid cell lines to examine b-catenin and WT1 protein expression. (haematologica.org)
WT1 is an RNA-binding protein, 6 and β-catenin has also been shown to bind RNA, 7 so to confirm this interaction was not indirect via RNA binding we repeated WT1 Co-IP (with or without CHIR99021) after first confirming complete digestion of RNA through RNase A pre-treatment of cell lysates ( Figure 1E ). (haematologica.org)
A) Immunoblot of myeloid leukemia cell lines showing the relative level of b-catenin (~92 kDa) and WT1 (~50 kDa) protein, with b-actin (42 kDa) used to assess protein loading. (haematologica.org)
Interestingly, exogenously overexpressed METTL16 differed from your endogenous protein in its relative affinity for RNA focuses on which prompted us to investigate METTL16's localization within the cell. (diferencias-entre.org)
Pan-cancer analysis and scRNA-seq data analysis were employed to forecast the association between Actin filament-associated protein 1 like 1 (AFAP1L1) and the development of tumors and endothelial cells. (biomedcentral.com)
MK2 were subjected to a knockdown by interfering RNA, the mRNA and protein levels of HuR in human pulmonary microvascular endothelial cells (HPMECs) were not affected. (biomedcentral.com)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cellular metabolic protein, was found to colocalize with viral RNA-dependent RNA polymerase (NS5) in JEV-infected cells. (biomedcentral.com)

The tumor microenvironment (TME) is a complex and integrated system composed not only of tumor cells but also various types of cells such as endothelial cells, fibroblasts, immune cells, and inflammatory cells, as well as soluble factors such as cytokines and chemokines [ 4 ]. (biomedcentral.com)
The morphology in the cells and the composition with the matrix in central chondrosarcoma propose par allels in between differentiation phases of tumor cells and of ordinary chondrocytes. (sirnalibrary.com)
Similarly both non-tumor and tumor cells of main ESCC predominantly indicated mRNA and the mRNA manifestation levels in tumors were higher than in ANGPT1 those in combined non-tumor cells in 3/6 (50%) of ESCC instances whose RNA was available (Supplementary Number S3B). (mdm-inhibitors.com)
Notably, many natural compounds have been found to effectively suppress NF-κB signaling, providing an experimentally validated molecular explanation for their functional outcomes in tumor cells ( 14 ). (spandidos-publications.com)

cIAP1 has an important role on the maintenance of endothelial cells and blood vessel homeostasis during the development of the vessels. (wikipedia.org)
We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)
These inflammatory factors are produced by various cell types in the central nervous system (CNS) such as microglia, astrocytes, endothelial cells, other glial cells, and peripherally derived immune cells. (biomedcentral.com)
CONCLUSIONS These results suggest that activation of VEGFR-1 by VEGF-A within the carcinoma, and activation of lymphatic endothelial cell VEGFR-3 by VEGF-D within the adjacent benign stroma may be important see more signaling mechanisms involved in the progression and subsequent metastatic spread of prostate cancer. (cdk-receptor.com)
The expression levels of FENDRR in hypoxic pulmonary artery endothelial cells (HPAECs) were detected by using quantitative real-time polymerase chain reaction (qRT-PCR) and fluorescence in situ hybridization (FISH). (biomedcentral.com)
Our investigation revealed that AFAP1L1 plays a crucial role in promoting the development of various tumors and demonstrates a strong correlation with endothelial cells. (biomedcentral.com)
Targeted suppression of AFAP1L1 specifically in endothelial cells in vivo proves effective in inhibiting tumor formation and ocular pathological neovascularization. (biomedcentral.com)
Inhibiting AFAP1L1 results in the inhibition of angiogenesis by suppressing the germination of endothelial tip cells through the YAP-DLL4-NOTCH axis. (biomedcentral.com)
This perturbation leads to anomalous endothelial cell functionality, disordered vascular structure, elevated microvascular density, and augmented permeability, which aim to supply more oxygen, nutrients, and metabolic waste to the abnormal growth of solid tissues [ 3 ]. (biomedcentral.com)
The endothelial cells in TME plays a crucial role in the occurrence and development of human cancer, making it a significant research topic in the field. (biomedcentral.com)
Endothelial cells and their deposited complex extracellular matrix will establish a vascular niche. (biomedcentral.com)
These endothelial cells not only supply nutrients and O 2 through the vascular system to facilitate tumor growth, but they also exhibit paracrine secretion, releasing stem and progenitor cell-active trophogens and endothelial-derived growth factors [ 5 ]. (biomedcentral.com)

Both proteins were ubiquitinated in the cytoplasm. (cdc.gov)
Many cell proteins continue to be phosphorylated throughout infection. (microbiologyresearch.org)
Similar to the other guard proteins, overexpression of HRP1 is toxic to cells and retains mRNAs in the nucleus. (uni-goettingen.de)
In order to obtain more detailed information regarding the interaction between host proteins and the defined viral nucleic acid and/or proteins, we investigated the host factors associating with the main replicase enzyme, NS5, in the JEV-infected cells using yeast-two hybrid screening. (biomedcentral.com)
For the purpose of this study we selected a set of proteins that were misregulated following silencing of TDP-43 and analysed their expression in a TDP-43-aggregation model cell line HEK293 Flp-in Flag-TDP-43-12x-Q/N F4L. (nature.com)
Likewise TIA1 proteins overexpression was seen in most of cancers cells weighed against regular mucosa (Supplementary Amount S2B). (mdm-inhibitors.com)
For example, matrix metalloproteinases (MMPs), such as MMP-2 and −9, have been shown to be typical NF-κB target proteins that are responsible for extracellular matrix breakdown and cell invasion ( 7 ). (spandidos-publications.com)
[ 15 ] The surface of the APC contains 2 peptide-binding proteins (ie, major histocompatability complex [MHC] classes I and II), which can stimulate cytotoxic T (T C ) cells, regulatory T (Treg) cells, and helper T (T H ) cells. (medscape.com)

This was experimentally determined by immunofluorescence microscopy and subcellular fractionations methods. (wikipedia.org)
As part of a continuing investigation of the properties of granule and plasma membranes of these cells, we are attempting to localize the saccharide residues of membrane glycoproteins and glycolipids by using ferritin conjugates of plant agglutinins whose binding can be monitored by electron microscopy (8). (docksci.com)
Transmission electron microscopy revealed cetuximab-IONP GBM cell binding and internalization. (oncotarget.com)

ERKi treatment of cells drives the poly-ubiquitylation and proteasome-dependent turnover of ERK2 and pharmacological or genetic inhibition of Cullin-RING E3 ligases prevents this. (babraham.ac.uk)
The principal mechanism of angiogenesis inhibition entails the regulation of tip cell behavior through the YAP-DLL4-NOTCH axis. (biomedcentral.com)
The effect of LY294002 inhibition on cell survival was determined by growing cells to 80% confluence followed by development factor deprivation for 48 h while in the presence of DMSO or 25 uM LY294002. (sirnalibrary.com)

Interestingly, amyloid-beta (A beta) soluble oligomers were able to induce upregulation of VDAC1 in a human neuroblastoma cell line, further supporting a correlation between A beta levels and VDAC1 expression. (cdk-receptor.com)
By tinkering with the speed and order of spins with a centrifuge, Claude could thus physically separate known subcellular structures into four rough fractions: large and heavy nuclei, medium-sized mitochondria, a smaller fraction he eventually termed "microsomes," and the freely soluble cytoplasm. (rockefeller.edu)

Further mechanistic research indicated that circHERC1 acted as a competing endogenous RNA of miR-142-3p to relieve the repressive effect of miR-142-3p on its target HMGB1, activating the MAPK/ERK and NF-κB pathways and promoting cell migration and invasion. (biomedcentral.com)

We leverage our method to uncover the regulation of gene expression involving RNA localization and transport in single cells. (riken.jp)
Our findings demonstrate that acquisition of MEK inhibitor resistance often occurs through gene amplification and can be suppressed by impeding cell cycle entry in drug. (babraham.ac.uk)
mRNA quality is controlled by multiple RNA surveillance machineries to reduce errors during gene expression processes in eukaryotic cells. (molcells.org)
Gene expression profiles have indicated that during progression chondrosar coma cells shift from a differentiated state in reduced grade tumors to a state additional very similar to early chondrogenic differentiation phases of mesenchymal precursor cells in higher grade tumors. (sirnalibrary.com)
When the iglB gene was disrupted IglA could not be detected in cell extracts of F. novicida , although IglC could be detected. (biomedcentral.com)

Starting with the first electron microscope image of an intact cell in 1945, these three (and many others) helped launch the modern discipline of cell biology. (rockefeller.edu)

Induction of CYP3A4 by rifampicin was significantly enhanced by the knockdown of NEAT1_2 or DAZAP1 in ShP51 cells and their parental HepG2 cells. (aspetjournals.org)

Genetic experiments using mouse embryonic fibroblast (MEF) cells derived from wild type or Nrf2 knockout mice revealed that the cells lacking Nrf2 exhibited markedly increased production of ROS and sensitivity to Cd cytotoxicity. (cdc.gov)
In fact, we were able to show that 3'-elongated mRNAs reached the cytoplasm in the hrp1-1 cft2-1 double mutant with cell fractionation experiments. (uni-goettingen.de)
Gain- and loss-of-function experiments were implemented to observe the impacts of circHERC1 on the growth, invasion, and metastasis of lung cancer cells in vitro and in vivo. (biomedcentral.com)

ER stress increases cIAP1 expression in cancer cells through the UPR pathway, that is why, the induction of cIAP1 is suggested to be important for cancerous cell survival under stress conditions. (wikipedia.org)
More importantly, we found that circHERC1 could bind FOXO1 and sequester it in the cytoplasm, adjusting the feedback AKT pathway. (biomedcentral.com)

Recent data demonstrate that the recruitment of dendritic cells to neoplastic lesions (and hence the priming of tumor-specific CD8 + T cells) is highly dependent on radiotherapy dose and fractionation through a mechanism that involves the accumulation of double-stranded DNA in the cytoplasm of cancer cells and consequent type I IFN release. (aacrjournals.org)
However, after the stimulation of TNF-α, silencing MK2 inhibited HuR accumulation to cytoplasm from nucleus in HPMECs. (biomedcentral.com)
Histiocytoses encompass a group of diverse proliferative disorders characterized by the accumulation and infiltration of variable numbers of monocytes, macrophages, and dendritic cells in the affected tissues. (medscape.com)

Chromatin immunoprecipitation (ChiP) assay revealed that Cd substantially increased the ARE occupancy of Nrf2 but not keap1, indicating Keap1 may recycle from the nucleus to the cytoplasm. (cdc.gov)

In healthy cells, Bax resides inactive in the cytosol because its COOH-terminal transmembrane region (TMB) is tucked into a hydrophobic pocket. (rupress.org)

Pluripotent stem cells can also be committed to produce dendritic cells. (medscape.com)
The importance of dendritic cells in presenting antigens to T and B lymphocytes is increasingly recognized. (medscape.com)
Dendritic cells appear to develop in several pathways. (medscape.com)
[ 11 ] Immature dendritic cells respond to GM-CSF (not to macrophage colony-stimulating factor [M-CSF]) and become committed to generating dendritic cells, which are "professional" antigen-presenting cells (APCs). (medscape.com)
[ 13 ] Dendritic cells are also efficient stimulators of B-cell lymphocytes. (medscape.com)
Effective induction of antigen-specific T-cell responses requires interaction between the dendritic cells and T lymphocytes to prime the latter cells for expansion and subsequent immune responses. (medscape.com)
Dendritic cells display a large amount of MHC-peptide complexes at their surface and can increase the expression of costimulatory receptors and migrate to the lymph nodes, spleen, and other lymphoid tissues, where they activate specific T cells. (medscape.com)

In the eukaryotic cells the production of ribosomes takes place in the nucleolus, a specialized subnuclear compartment where the RNA Polymerase I (RNA Pol I) catalyses the ribosomal RNA synthesis (rRNA). (biomedcentral.com)

Using cell fractionation and immunoprecipitation, we further demonstrated that Nrf2 and Keap1 were both detected in the cytoplasm and nucleus. (cdc.gov)
Immunoprecipitation assays using PXR-overexpressing HepG2 (ShP51) cells revealed that PXR interacts with not only DAZAP1 but also NEAT1_2, a long noncoding RNA included in the paraspeckle, and that the interaction between PXR and NEAT1_2 was disassociated by rifampicin. (aspetjournals.org)

This suggests, unexpectedly, that in the granule membrane carbohydrate residues face the cytoplasm. (docksci.com)
The nucleolus is a subnuclear, non-membrane bound domain that is the hub of ribosome biogenesis and a critical regulator of cell homeostasis. (biomedcentral.com)
Other intracellular pathogens, such as Listeria monocytogenes , degrade the phagosomal membrane and escape into the cytoplasm to replicate freely [ 8 ]. (biomedcentral.com)

Interestingly, we found that myeloid-specific TonEBP deletion blocked the LPS-induced microglia activation and subsequent neuronal cell death and memory loss. (biomedcentral.com)
Interestingly, JPX and Twist1 were coordinately upregulated in lung cancer tissues and cells. (biomedcentral.com)

RT-qPCR and western blotting were conducted to detect the expression levels of lncRNA JPX and Twist1 in lung cancer cell lines and tissues. (biomedcentral.com)
Here we demonstrate that Tudor staphylococcal nuclease (SND1 or TSN) is overexpressed in NSCLC cell lines and tissues, and is important for maintaining NSCLC chemoresistance. (oncotarget.com)

It directly ubiquitinates RIP1 and induces constitutive RIP1 ubiquitination in cancer cells. (wikipedia.org)

For a comprehensive history of cell biology, particularly at Rockefeller University, I refer the reader to "Entering an Unseen World" by our very own Carol Moberg. (rockefeller.edu)
LLPS, which was originally studied in the field of physical chemistry, has been extended to the field of cell biology since the 2010s. (aspetjournals.org)
Improved understanding of the pathology of histiocytic disorders requires knowledge of the origins, biology, and physiology of the cells involved. (medscape.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Tumor suppressors, oncogenes and alternatively deregulated upstream signalling pathways can directly influence the RNA polymerase I activity inducing hyper activation of rRNA transcription in cancer cells. (biomedcentral.com)
Our findings indicate that circHERC1 facilitates the invasion and metastasis of NSCLC cells by regulating the miR-142-3p/HMGB1 axis and activating the MAPK/ERK and NF-κB pathways. (biomedcentral.com)
The correl ation of the differentiation stage of chondrosarcoma cells for the degree of malignancy in the tumors indi cates that signaling pathways that management standard chondrogenesis may perhaps possess a regulatory perform within the progression of those tumors. (sirnalibrary.com)
In the current study, the anti‑invasive activities and associated signaling pathways of celastrol were determined in ovarian cancer cells. (spandidos-publications.com)

GBM stem-like cells (GSCs) present in tumors are felt to be highly tumorigenic and responsible for tumor recurrence. (oncotarget.com)
Rapid growth and division of cells in tumors are correlated with intensive nucleolar metabolism as a response to oncogenic factors overexpression. (biomedcentral.com)
With regard to cancer treatment, celastrol has been shown to exert considerable cell-killing effects and to shrink xenografted tumors in tissue and animal models of various malignancies including cancers of the prostate ( 16 - 18 ), breast ( 19 ), liver ( 20 , 21 ) and lung ( 22 , 23 ). (spandidos-publications.com)

GBM stem-like cells (GSCs) represent a subpopulation of the tumor which is responsible for tumor initiation, progression, and re-growth after chemoradiation [ 5 , 6 ], as well as supporting tumor vessel growth and function [ 7 ]. (oncotarget.com)
Pyroptosis is a form of programmed cell death involved in the pathophysiological progression of hypoxic pulmonary hypertension (HPH). (biomedcentral.com)
2019 ). However, the reported lncRNAs in HPH are all focused on the cytoplasm of PASMCs, and the specific lncRNAs located in the nucleus, especially in PAECs involved in HPH progression and their related regulatory mechanism, remain largely unknown. (biomedcentral.com)

Desk 1 Association between clinicopathological features and TIA1 appearance Desk 2 Cox proportional threat regression evaluation for overall success Appearance of TIA1 in ESCC cell lines mRNA overexpression weighed against the esophagus was also discovered in 30 of 45 ESCC cell lines by quantitative real-time PCR (qPCR Supplementary Amount S2A). (mdm-inhibitors.com)

Background: An increasing number of studies on stem cells suggests that the therapeutic effect they exert is primarily mediated by a paracrine regulation through extracellular vesicles (EVs) giving solid grounds for stem cell EVs to be exploited as agents for treating diseases or for restoring damaged tissues and organs. (123dok.org)
Due to their capacity to differentiate in all embryonic germ layers, amnioticﬂuid stem cells (AFCs), represent a highly promising cell type for tissue regeneration, which however is still poorly studied and in turn underutilized. (123dok.org)
The rapid advances in theﬁeld of regenerative medicine have identiﬁed novel sources of stem cells with potential for therapy. (123dok.org)
In addition to fully differentiated cells, precursors and multipotent stem like cells have been described within AF[3]. (123dok.org)
In addition, very recent data indicate that the structural contribution of stem cells (SCs) to regenerated tissues seems to be limited, and that rather the stimulation of local healing processes plays an important role in regeneration [6]. (123dok.org)
1 Wnt/b-catenin is also known to drive the emergence and maintenance of leukemia stem cells in AML. (haematologica.org)
m6A offers been shown to play a role in a number of physiological processes including embryonic stem cell differentiation, circadian rhythms, response to hypoxia and additional stressors, and is implicated in many different aspects of malignancy [1, 9, 16, 18C27]. (diferencias-entre.org)
Normal histiocytes originate from pluripotent stem cells, which can be found in bone marrow. (medscape.com)
Committed stem cells can mature to become antigen-processing cells, with some possessing phagocytic capabilities. (medscape.com)

Melanoma in particular exhibits a high incidence of activating BRAF and NRAS mutations and such cells are addicted to the activity of these mutant oncoproteins. (babraham.ac.uk)
A non-polar iglA deletion mutant was defective for growth in mouse macrophage-like cells, and in cis complementation largely restored the wild type macrophage growth phenotype. (biomedcentral.com)

We observed a statistically significant correlation between b-catenin and WT1 expression across 16 myeloid cell lines, with 50% (8/16) co-expressing b-catenin and WT1 to varying degrees ( Figure 1A , B). There was no particular association of this correlation with cell line morphology or genotype. (haematologica.org)

TonEBP deficiency blocked the LPS-induced expression of pro-inflammatory cytokines and enzymes in association with decreased activity of NF-κB in BV2 cells. (biomedcentral.com)
In settings of pathogenesis, inflammatory stimuli can prime microglial cell leading to a constant production of inflammatory cytokines and chemokines which, in turn, maintain activation of the primed cells [ 4 ]. (biomedcentral.com)

Microglia are brain-resident myeloid cells involved in the innate immune response and a variety of neurodegenerative diseases. (biomedcentral.com)
In animals, TonEBP was deleted from myeloid cells using a line of mouse with floxed TonEBP. (biomedcentral.com)

Selumetinib causes long-term G1 arrest accompanied by reduced expression of DNA replication and repair genes, but cells stochastically re-enter the cell cycle during treatment despite continued repression of pERK1/2. (babraham.ac.uk)
Replication of flavivirus RNA takes place in the cytoplasm by an asymmetric and semiconservative mode resulting in 10 to 100-fold greater plus strands compared to minus strands [ 5 ]. (biomedcentral.com)

Several courses of treatment are focused on the removal of the IAPs to induce cells cytotoxicity. (wikipedia.org)

Those cells show high plasticity and the capacity to differentiate into the three types of germ layer cells, making them ideal for ap-plications in regenerative medicine[4], eventually banked and used for treatments. (123dok.org)

Here, Neuregulin 1 mediated proliferative stimuli, promotes ErbB3 relocalization from the nucleolus to the cytoplasm and increases pre-rRNA synthesis. (biomedcentral.com)

The ATP-dependent RNA helicase up-frameshift 1 (UPF1) is a key player in NMD that is mostly prevalent in the cytoplasm. (molcells.org)
Figure 3B indi cates that FET cells had a time dependent raise in DNA fragmentation throughout GFDS as when compared with FET DN cells. (sirnalibrary.com)

Discussion：Reduced expression of DNAJC3-AS1 was observed after miR-140 overexpression in AML cells. (mjhid.org)

These findings suggest a novel role of UPF1 in transcription elongation-coupled RNA machinery in the chromatin, as well as in translation-coupled NMD in the cytoplasm. (molcells.org)

Address correspondence to C. Borner, Institute of Molecular Medicine and Cell Research, Center for Biochemistry and Molecular Cell Research, Albert-Ludwigs-University Freiburg, Stefan-Meier Strasse 17, D-79104 Freiburg, Germany. (rupress.org)

Cell Death & Differentiation. (wikipedia.org)

Besides, LINC01980 promoted HCC cells migration and invasion. (researchsquare.com)
Cell migration was detected by wound healing and Transwell assays, while cell invasion was detected by a Matrigel‑coated Transwell method. (spandidos-publications.com)

Non-small cell lung cancer (NSCLC), the major lung cancer subtype, is characterized by high resistance to chemotherapy. (oncotarget.com)
Downregulation of TSN by RNAi in NSCLC cells led to strong potentiation of cell death in response to cisplatin. (oncotarget.com)
Downregulation of S100A11 by RNAi resulted in enhanced sensitivity of NSCLC cells to cisplatin, oxaliplatin and 5-fluouracil. (oncotarget.com)
NSCLC is divided into adeno-, squamous- and large cell lung carcinomas, accounting for about 40%, 30% and 10-15% of all lung cancer cases, respectively. (oncotarget.com)
Non-small cell lung cancer (NSCLC) is one of the most common malignant cancers. (biomedcentral.com)
According to the latest statistics, lung cancer, of which 85% are non-small cell lung cancers(NSCLC), is the most commonly diagnosed cancer and the leading cause of cancer death in both men and women, the incidence and mortality of lung cancer have increased due to environmental pollution and an increasing number of smokers in recent years [ 1 ].The understanding of genetic alterations that drive NSCLC is evolving. (biomedcentral.com)

These findings suggest that lncRNA JPX, a mediator of Twist1 signaling, could predispose lung cancer cells to metastasis and may serve as a potential target for targeted therapy. (biomedcentral.com)

In epithelial cells, VEGF-A and VEGFR-1 expression was higher in tumor tissue compared to benign tissue. (cdk-receptor.com)
Lectin microarrays using LecChip™ allows the highly sensitive analysis of carbohydrates in various sample types (purified and crude samples, e.g. cell/tissue extracts, living cells, virus particles, cell culture supernatants, serum, urine). (bmgrp.com)

CircPAPD4 expression was low in BC tissues and cells. (biomedcentral.com)

Colony formation assays exposed that TIA1a-expressing cells created more colonies than control and TIA1b-expressing. (mdm-inhibitors.com)

Despite this, clinical responses are typically transient as tumour cells develop resistance. (babraham.ac.uk)

Anatomy53

Organisms16

Chemicals and Drugs82

Analytical, Diagnostic and Therapeutic Techniques and Equipment55

Phenomena and Processes52

Disciplines and Occupations4

Technology, Industry, Agriculture2

Information Science3

Health Care2

Subcellular fractionation5

Found in the cytoplasm1

Nucleus and the cytoplasm2

Cytoplasmic5

Proliferation19

Apoptosis8

Biochemical4

Nuclear10

Nucleic acids1

Protein16

Tumor cells4

Endothelial15

Proteins8

Microscopy3

Inhibition3

Soluble2

Mechanistic1

Gene5

Electron1

Knockdown1

Experiments3

Pathway2

Accumulation3

Assay1

Resides1

Dendritic cells7

Eukaryotic cells1

Immunoprecipitation2

Membrane3

Interestingly2

Lines and tissues2

Ubiquitination1

Biology3

Pathways5

Tumors3

Progression3

MRNA1

Stem9

Mutant2

Correlation1

Cytokines2

Myeloid cells2

Replication2

Cytotoxicity1

Differentiate1

Increases1

Dependent2

Overexpression1

Transcription1

Biochemistry1

Differentiation1

Migration2

NSCLC6

Metastasis1

Tissue2

Tissues and cells1

Assays1

Transient1