• Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
  • therapeutic monoclonal antibodies targeting IL-17A, both IL-17A and IL-17F, the IL-17 receptor, or IL-23 are highly effective in some of these diseases. (nature.com)
  • These studies and others detailing pathological roles of IL-17 in human diseases eventually culminated in the development of monoclonal antibodies (mAbs) that target IL-17A, both IL-17A and IL-17F, IL-17RA, or IL-23, a cytokine produced by innate immune cells that promotes the expansion of T H 17 cell populations. (nature.com)
  • Ongoing and planned clinical trials are assessing various treatment strategies, such as the combination of purine analogues and various anti-CD20 monoclonal antibodies, recombinant immunotoxins targeting CD22 (e.g., moxetumomab pasudotox), BRAF inhibitors, such as vemurafenib, and B-cell receptor signaling inhibitors, such as ibrutinib, which is a Bruton's tyrosine kinase inhibitor. (nih.gov)
  • Subsequently, we used monoclonal antibodies directed against the different domains of PrPC in order to evaluate their role in the uptake process. (sissa.it)
  • In order to gain more insight into the tumour-host relationship, the present study was conducted to characterize the in situ phenotype of the lymphocytic infiltrate surrounding tumour tissue of BCC through the use of monoclonal antibodies against B and T cell populations. (who.int)
  • Description Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal Antibody (11108). (qedbio.com)
  • Primary Antibody: Rabbit Anti-Hsp70 Polyclonal Antibody (11108) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. (qedbio.com)
  • Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (11108) at 1:50000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. (qedbio.com)
  • Description Western blot analysis of Human, Rat brain cell lysates showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (11108). (qedbio.com)
  • Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (11108) at 1:10000 for 2 hours at RT. (qedbio.com)
  • Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (11108) at 1:100 for 1 hour at RT. (qedbio.com)
  • Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (11108) at 1:1000 for 2 hours at RT. (qedbio.com)
  • Description Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BNIP3 Polyclonal Antibody (2290). (qedbio.com)
  • Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (2290) at 1:100 for 60 min at RT. (qedbio.com)
  • Description Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (2290). (qedbio.com)
  • Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (2290) at 1:1000 for 1 hour at RT. (qedbio.com)
  • Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT. (qedbio.com)
  • Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. (qedbio.com)
  • Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. (qedbio.com)
  • Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-LRRK2 Antibody (#ANR-102), (1:400), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. (alomone.com)
  • A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BNIP3 Antibody (D) Composite. (qedbio.com)
  • The cell samples were stained with ERα36 antibody and DAPI. (nih.gov)
  • However, there are several remaining uncertainties concerning the mechanism by which antibodies afford protection against self-propagating protein conformations. (frontiersin.org)
  • Common mechanisms that may contribute to protection against self-propagating protein conformations include blocking the entry of protein "seeds" to cells, clearance of immune complexes by microglia, and the intracellular protein degradation pathway initiated by cytoplasmic antibodies via the Fc receptor TRIM21. (frontiersin.org)
  • This antibody recognizes the STAT5a protein from a variety of species including human, mouse, and rat and it reacts specifically with the STAT5a protein and does not exhibit any cross-reactivity with the highly related STAT5b protein. (thermofisher.com)
  • Anti-LRRK2 Antibody (#ANR-102) is a highly specific antibody directed against an epitope of the mouse protein. (alomone.com)
  • Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
  • Immunohistochemistry (IHC) was used to localize Cfh protein expression, and western blots were used to characterize CFH antibodies used for IHC. (molvis.org)
  • Our CFH antibodies recognized purified mouse Cfh protein, serum Cfh protein in wild-type C57BL/6, BALB/c, and 129/Sv, and showed an absence of the Cfh protein in the serum of Cfh −/− mice. (molvis.org)
  • Depolymerization of microtubules inhibits the export of a newly synthesized transmembrane protein from the Golgi apparatus to the plasma membrane and simultaneously blocks membrane growth. (rupress.org)
  • The protein expression profiles of cytoplasm and membrane/nuclei fractions were checked using protein two-dimensional electrophoresis (2-DE). (hindawi.com)
  • Antibody -- Any of the protein molecules produced by specialized immune system cells (B cells) that can recognize and bind to a particular foreign antigen. (nih.gov)
  • The person's immune system then recognizes the viral spike protein as foreign and produces antibodies to eliminate it. (nih.gov)
  • Experiments in animal models of tau and αS pathology have demonstrated that the passive transfer of anti-tau or anti-αS antibodies induces a reduction in the levels of assembled proteins. (frontiersin.org)
  • This review will discuss current understanding of how antibodies and their receptors can be brought to bear on proteins involved in neurodegeneration. (frontiersin.org)
  • Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. (thermofisher.com)
  • The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. (qedbio.com)
  • The principal methods in this work were immunohistochemistry and immunofluorescence with validated antibodies against structural and channel proteins of satellite cells and against inflammatory proteins. (biomedcentral.com)
  • Be aware that some proteins can be expressed on the surface of the cell, in the cytoplasm, internal organelles, the nucleus, or in multiple compartments simultaneously. (biolegend.com)
  • Notably, while both a-synuclein and tubulin are cytosolic proteins with other functions (tubulin is the building block of microtubules), they appear to double as membrane-associated proteins, displaying high affinity to mitochondrial membranes. (nih.gov)
  • The following product was used in this experiment: CD26 Monoclonal Antibody (236.3) from Thermo Fisher Scientific, catalog # MA2607, RRID AB_223591. (thermofisher.com)
  • The following product was used in this experiment: STAT5 alpha Monoclonal Antibody (ST5a-2H2) from Thermo Fisher Scientific, catalog # 13-3600, RRID AB_2533013. (thermofisher.com)
  • LRRK2 Blocking Peptide (#BLP-NR102) is the original antigen used for immunization during Anti-LRRK2 Antibody (#ANR-102) generation. (alomone.com)
  • Anti-LRRK2 Antibody, preincubated with LRRK2 Blocking Peptide (#BLP-NR102). (alomone.com)
  • Antigen Preadsorption Control 1 µg peptide per 1 µg antibody. (alomone.com)
  • These peptide usually contains the epitope recognized by the antibody. (affbiotech.com)
  • The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. (affbiotech.com)
  • A blocking peptide competition assay confirmed that the mitochondria-associated ERα36 signal detected by confocal analysis was specific for ERα36. (nih.gov)
  • This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. (thermofisher.com)
  • Explain why the antibody binds to the transcription factor. (ict4water.eu)
  • Will blocking the formation of mitochondrial ROS (reactive oxygen species) with Mito-Q (mitochondria-targeted ubiquinone) be an effective therapy to block the development of endothelial dysfunction and lower blood presure in mice exposed to chronic unpredictable stress? (sc.edu)
  • In hypertension induced by chronic unpredictable stress, oxidative stress leads to release of mitochondrial DNA (mtDNA) into the cytoplasm. (sc.edu)
  • Regulation of mitochondrial outer membrane permeability is crucial in normal metabolite exchange between mitochondria and cytoplasm (thus controlling respiration), and in apoptosis, by releasing apoptogenic factors into the cytosol. (nih.gov)
  • When cells were double-stained with an ERα36 antibody and a mitochondrial-specific dye, MitoTracker, the PCC for the two channel signals were both greater than 0.75, indicating strong correlation between ERα36 and mitochondrial signals in the two cell lines. (nih.gov)
  • In particular, most of the antibody reagents used do not have clear references for the host organism, the exact identity of the antigen used, and the state of antibody purification of the final product. (molvis.org)
  • Other cells, such as the B lymphocytes which will eventually produce Antibodies, will return to the G1 phase when stimulated by an antigen. (angelfire.com)
  • Antigen -- A molecule whose shape triggers the production of antibodies, or immunoglobulins, that will bind to the antigen. (nih.gov)
  • Parallels will be made to antibody-mediated protection against classical viral infections. (frontiersin.org)
  • Viral infection of vertebrates leads to the production of antibodies, and some of these can block virus infection by a process that is called neutralization. (virology.ws)
  • Antibodies can neutralize viral infectivity in a number of ways: they may impair virus entry into cells, and even cause degradation of the capsid in the cytoplasm. (virology.ws)
  • Samples have been examined for HIV-1 antibodies, western blot, viral load, HIV-1 genotypes by means of direct sequencing, and antiretroviral resistance testing. (genscanner.com)
  • as payloads in antibody-drug conjugates, are typically labeled by their membrane permeability and the extent of cytotoxic bystander exercise on neighboring cells after focused supply. (genscanner.com)
  • Moreover, damaged mtDNA can be secreted from mitochondria to the cytoplasm and extracellular space via exosomes. (nih.gov)
  • A panel of antibodies was used to reveal the cytoplasm of satellite cells (glutamine synthetase, S100, metabotropic glutamate receptors 2/3, excitatory amino acid transporter 1, ATP-sensitive inward rectifier potassium channel 10, and cytosolic ferritin), gap junctions (connexin 43), basement membranes (laminin), mitochondria (ATP synthase subunit beta and frataxin), and monocytes (CD68 and IBA1). (biomedcentral.com)
  • B. Staining in the substantia nigra pars compacta shows LRKK2 staining (green) in nuclei and cytoplasm of several cells (arrows). (alomone.com)
  • Granulocytes are distinguished from agranulocytes by their nucleus shape (lobed versus round, that is, polymorphonuclear versus mononuclear) and by their cytoplasm granules (present or absent, or more precisely, visible on light microscopy or not thus visible). (wikipedia.org)
  • This regulation includes checks on the division of the nucleus and the cytoplasm. (angelfire.com)
  • Antibodies can perform diverse functions, including blocking of ligand/receptor binding, immune functions, and removal of target from the cell surface by ingestion or secretion. (medscape.com)
  • Two principal methods to inhibit EGFr have been identified and are being used in clinical trials, small molecule inhibitors of EGFr and antibodies to EGFr. (medscape.com)
  • Two small molecule inhibitors, ZD1839 and erlotinib HCl, OSI-774, and 1 antibody, C225 (IMC-C225) have been evaluated in SCCHN. (medscape.com)
  • This LIFR approach has allowed us to test the role of host pathways in the mechanism of action of BRAF inhibitors and to combine BRAF inhibitors with various antibody therapies designed to drive T cell antitumor activity in a model in which BRAF inhibition does not cause major tumor regressions, allowing examination of synergistic roles of host pathways and direct anti-melanoma activity. (thetechnoant.info)
  • Do not freeze antibodies conjugated to fluors, as this can cause precipitation or aggregation of the antibody. (biolegend.com)
  • Low or no signals from your antibody staining, characterized as no signal above the isotype control or unstained control, can be due to a number of different factors depending on your experiment. (biolegend.com)
  • Sections were blocked by 5% bovine serum albumin and incubated with the primary antibody against CA125 (Boiss, China), HE4 (Boiss, China) and B7-H4 (Boiss, China) at 4 °C overnight. (biomedcentral.com)
  • Whereas TMUV doesn't trigger illness in people, it has been reported that antibodies in opposition to TMUV have been present in serum samples from duck farmers, and thus knowledge on TMUV an infection in people is proscribed, and the pathogenesis is unclear. (genscanner.com)
  • Blocking EGFr activation enhances tumor specific destruction by standard chemotherapy agents and radiotherapy. (medscape.com)
  • Impaired IF production in pernicious anemia occurs as a result of autoimmune destruction of parietal cells, which secrete IF, or the development of auto-antibodies targeted against IF itself. (medscape.com)
  • Our Foxp3 antibodies are optimized for use with our True-Nuclear™ Transcription Factor Buffer Set and may not work adequately with buffers from other vendors. (biolegend.com)
  • Nuclear division (KARYOKINESIS) and division of the cytoplasm (CYTOKINESIS). (angelfire.com)
  • By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific. (affbiotech.com)
  • Representative image of Duolink in situ proximity ligation assay using anti-α1-tubulin and anti-VDAC antibodies in human neuroblastoma cells. (nih.gov)
  • 1. Water moves into the cells/cytoplasm by. (ict4water.eu)
  • A. LRKK2 staining (green) of striatum sections is detected in the cytoplasm of several striatal cells (arrows). (alomone.com)
  • Immunohistochemical techniques using monoclonal antibody recognition of B and T cells have been shown to solve this problem in various pathological conditions [9-11]. (who.int)
  • Upon their return they will continue in the cycle and eventually become plasma cells which will produce antibodies. (angelfire.com)
  • ht-UtLM) cells, we conducted systematic confocal microscopy and subcellular fractionation analysis using ERα36 antibodies. (nih.gov)
  • In ht-UtLM cells (2), the ERα36 signals were detected mostly in a robust network structure within the cytoplasm. (nih.gov)
  • Immunology -- The branch of biomedicine concerned with the structure and function of the immune system, the bodily distinction of self from nonself, and laboratory techniques involving the interaction of antigens with specific antibodies. (nih.gov)
  • Similarly to what was reported for splicing factors, blocking splicing or transcription leads to the accumulation of FMR2 in enlarged, rounded speckles. (nih.gov)
  • Transcriptional factor/antibody) has a, Suggest how single-stranded cDNA could prevent transcription of the P34 gene. (ict4water.eu)
  • A). Furthermore, histochemical staining demonstrated RCAS1 expression in the cytoplasm of bone marrow macrophages. (elsevierpure.com)
  • therapy with PLX4720 and anti-CCL2 or agonistic anti-CD137 antibodies demonstrated significant antitumor activity in mouse transplant and de novo tumorigenesis models. (thetechnoant.info)
  • Indeed, the absence of PrPC does not block completely the entrance of tau fibrils in the cytoplasm. (sissa.it)
  • Phospho-IKK alpha/ beta (Ser176/Ser177) Antibody detects endogenous levels of IKK alpha/ beta only when phosphorylated at Ser176/Ser177. (affbiotech.com)
  • C225 prevents binding of activating cytokines to the EGFr and causes EGFr movement into the cytoplasm, where it is sequestered and unavailable. (medscape.com)
  • Will antibodies to IL-1(beta) (interleukin-1 beta) and IL-18 (interleukin-18) block the negative effect of these cytokines on the endothelium? (sc.edu)
  • For cytokines, secretion should be blocked using Monensin or Brefeldin A . This prevents cytokines from being secreted into the environment. (biolegend.com)
  • Most patients show an excellent long-term response to treatment with single-agent cladribine or pentostatin, with or without the addition of an anti-CD20 monoclonal antibody such as rituximab. (nih.gov)