DiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
Image CytometryFlow CytometryCytophotometryPloidiesAneuploidyDNA, NeoplasmImage Processing, Computer-AssistedLaser Scanning CytometryMicroscopyHypogravityDNA
ApoptosisEnzyme linkeTranswellViability and cellMicroarrayAnalyzerAnalyzeColony formationCellularReal-TimTransfectionXenograftTransmission electron mIncorporationAnalysisQuantificationSpecificityFluorescenceDetectionResultsFluorescentPeripheral bloodWesternCharacteristicsReporterMigrationExperimentsEfficiencyExpressionCellsMainSignificantlyBasicApplicationsTechnologyInstrumentsDataScientistsTechnologiesSoftwareActivityAbility to performDevelopmentTestHigh
Apoptosis5
  • Here, we determined the effect of Jiyuan oridonin A (JOA), an ent -kaurene diterpenoid compound, on the differentiation blockade in imatinib-sensitive, particularly, imatinib-resistant CML cells with BCR-ABL-T315I mutation by cell proliferation assay, apoptosis analysis, cell differentiation analysis, cell cycle analysis and colony formation assay. (jcancer.org)
  • Proven by the use of apoptosis-resistant cellular models and autophagic assays, such isoquinoline alkaloid-induced cytotoxic effect involves energy- and autophagy-related gene 7 (Atg7)-dependent autophagy that resulted from direct activation of AMP activated protein kinase (AMPK). (oncotarget.com)
  • MTT analysis, PI/Annexin V staining, transwell and metabolic flux assays were performed to examine the effects of C1QBP on proliferation, apoptosis, migration, invasion, and oxidative phosphorylation of HCC cells. (jcancer.org)
  • Loss-of-function assays showed that proliferation, migration and invasion of HCC cells were mitigated while cell apoptosis was augmented upon the loos of C1QBP. (jcancer.org)
  • Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling and Hoechst 33342 staining, and quantified by flow cytometry. (wjgnet.com)
Enzyme linke2
  • In addition, soluble CD163 (sCD163) was determined by enzyme linked immunosorbent assay. (biomedcentral.com)
  • The iPSC lines were differentiated toward mature cortical neurons, and AD pathological hallmarks were analyzed by RT-qPCR, enzyme-linked immunosorbent assay, and Western blotting methods. (biomedcentral.com)
Transwell2
  • Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
  • Transwell assay was performed to measure cell invasion. (bvsalud.org)
Viability and cell2
  • Collectively, both arms of this Module add scientific value to the work of vision scientists by reducing the time and effort required for basic cell culture assays such as viability and cell cycle assessments. (utsouthwestern.edu)
  • MATERIALS AND METHODS: Cell viability assay and EDU assay were used for detecting of cell viability and cell proliferation. (bvsalud.org)
Microarray1
  • In a previous study using a microRNA (miRNA/miR) microarray assay, we demonstrated that miR-133b-5p was upregulated in response to hypoxic preconditioning (HPC). (spandidos-publications.com)
Analyzer2
  • Uses for flow cytometry include: Cell counting Cell sorting Determining cell characteristics and function Detecting microorganisms Biomarker detection Protein engineering detection Diagnosis of health disorders such as blood cancers Measuring genome size A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. (wikipedia.org)
  • Cell counting kit 8, 5-Bromo-2-deoxyuridine, 5-ethynyl-2'-deoxyuridine (EdU) incorporation, flow cytometry analysis, western blotting and seahorse XFe24 extracellular flux analyzer assays showed that paeonol had a significant inhibitory effect against A549 cell proliferation and glycolysis. (cjphysiology.org)
Analyze4
  • He described the graphical interface the group developed-a logic map designed to analyze the data from functional live-cell assays that incorporate fluorescent markers. (genengnews.com)
  • Overall the use of flow cytometry to analyze individual cell populations and evaluate the effects of drugs on cell function allows you "to ask the what-if questions" and to begin to integrate pathways, he said. (genengnews.com)
  • Multiplex assays, with their ability to simultaneously analyze multiple analytes, provide efficient disease diagnosis, patient stratification, and monitoring. (polarismarketresearch.com)
  • Modern flow cytometers are able to analyze many thousands of particles per second, in "real time" and, if configured as cell sorters, can actively separate and isolate particles with specified optical properties at similar rates. (wikipedia.org)
Colony formation1
  • The biological function of LIPH-4 was examined in cell and animal studies, applying CCK-8, EdU, colony formation and flow cytometry assays as well as xenograft model experiments. (researchsquare.com)
Cellular2
  • The deposition of Cu in lysosomes isolated from macrophages was also determined by ICP-MS. Cell viability (metabolic activity) was assessed using the Alamar Blue assay, and oxidative stress was monitored by a variety of methods including a luminescence-based assay for cellular glutathione (GSH), and flow cytometry-based detection of mitochondrial superoxide and mitochondrial membrane potential. (biomedcentral.com)
  • The Tali Image-based Cytometer is a 3-channel benchtop cytometer that uses image analysis to perform basic cellular assays and cell counting on cells in suspension. (epfl.ch)
Real-Tim1
  • The circBACH1 and miR-217 expression levels were detected using quantitative real-time PCR (RT-qPCR) and Immunohistochemistry (IHC) assays in BC tissues and precancerous tissues of BC patients. (biomedcentral.com)
Transfection2
  • and a Nexcelom Cellometer for rapid determination of cell number, cell viability, transfection efficiency, and cell cycle assays. (utsouthwestern.edu)
  • Transient transfection assay was used for gene silence and overexpression. (bvsalud.org)
Xenograft1
  • Xenograft tumors and bioluminescent imaging were used for in vivo experiments. (bvsalud.org)
Transmission electron m1
  • Flow cytometry, nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) were used to characterize apoVs. (biomedcentral.com)
Incorporation1
  • The incorporation of a flow cytometer and a cell sorter also reduces the cost associated with recurrent use of the main flow cytometry facility on campus. (utsouthwestern.edu)
Analysis14
  • At CHI's recent "High-Content Flow Cytometry" meeting, Dr. Robinson talked about the ability to automate flow cytometry-based high-content screens and data processing to perform multiplexed high-content analysis in real-time, running multiple assays and samples in parallel, and to miniaturize the technology to conserve resources and reduce costs. (genengnews.com)
  • Using this approach, a biological sample can be analyzed in parts-exploiting the ability to separate individual cells for analysis using flow cytometry-with the data then combined to piece together the complete picture. (genengnews.com)
  • Automation of the entire process-from sample preparation, to running complex assays, to data analysis-makes it possible to perform functional phenotypic profiling of cell populations efficiently. (genengnews.com)
  • In addition, Dr. Robinson presented an example of multicolor flow analysis to demonstrate the ability to perform cytomics studies for pathway analysis. (genengnews.com)
  • The increasing use of automation and miniaturization is lowering the cost of flow cytometry-based high-content analysis in primary and secondary drug screening, making it possible to run larger screening campaigns. (genengnews.com)
  • The flow cytometer is equipped with multiple lasers for analysis of multi-fluorescent labelled cell populations and acoustical focusing for improved cell separations. (utsouthwestern.edu)
  • The effects of Pts on hepatotoxicity were measured by commercial kits, Hematoxylin and Eosin (H&E) straining, TUNEL assay, Western blot analysis, and Flow cytometry assay. (karger.com)
  • Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
  • Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
  • Multiplex assays play a critical role in this field by allowing simultaneous analysis of multiple biomarkers associated with specific diseases or conditions. (polarismarketresearch.com)
  • The activation of caspase‑8 and caspase‑3 were measured by western blot analysis to detect the cleaved fragments as well as a colorimetric assay. (spandidos-publications.com)
  • citation needed] A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • Cell growth assay, cell cycle analysis, FACS, JC-1 staining, annexin V staining and immunoblotting were used to study the efficacy of ST on cancer cells. (biomedcentral.com)
  • The relationship between FENDRR and dynamin-related protein 1 (DRP1) was explored using bioinformatics analysis, Chromatin Isolation by RNA Purification (CHIRP), Electrophoretic mobility shift assay (EMSA) and Methylation-Specific PCR (MSP) assays. (biomedcentral.com)
Quantification2
  • These advancements enable the detection and quantification of numerous analytes in a single sample, reducing the time and cost associated with traditional single-analyte assays. (polarismarketresearch.com)
  • A flow cytometer is similar to a microscope, except that, instead of producing an image of the cell, flow cytometry offers high-throughput, automated quantification of specified optical parameters on a cell-by-cell basis. (wikipedia.org)
Specificity2
  • Describing the benefits of using flow cytometry to study disease specificity as it relates to cell types and signaling pathways, Dr. Kritzik reported that it is possible to measure at least 10 parameters in 5,000-10,000 single cells per second. (genengnews.com)
  • The specificity of the assay is confirmed by a panel of clinically relevant non-MTB bacteria, and the clinical utility is demonstrated by the measurements in MTB-positive patient specimens. (cdc.gov)
Fluorescence5
  • The first fluorescence-based flow cytometry device (ICP 11) was developed in 1968 by Wolfgang Göhde from the University of Münster, filed for patent on 18 December 1968 and first commercialized in 1968/69 by German developer and manufacturer Partec through Phywe AG in Göttingen. (wikipedia.org)
  • Soon after, flow cytometry instruments were developed, including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics), the PAS 8000 (1973) from Partec, the first FACS (fluorescence-activated cell sorting) instrument from Becton Dickinson (1974), the ICP 22 (1975) from Partec/Phywe and the Epics from Coulter (1977/78). (wikipedia.org)
  • citation needed] The original name of the fluorescence-based flow cytometry technology was "pulse cytophotometry" (German: Impulszytophotometrie), based on the first patent application on fluorescence-based flow cytometry. (wikipedia.org)
  • At the 5th American Engineering Foundation Conference on Automated Cytology in Pensacola (Florida) in 1976 - eight years after the introduction of the first fluorescence-based flow cytometer (1968) - it was agreed to commonly use the name "flow cytometry", a term that quickly became popular. (wikipedia.org)
  • Western blot, Lactate dehydrogenase (LDH) release assay, Annexin V-FITC/PI double staining, Hoechst 33342/PI fluorescence staining and Caspase-1 activity assay were used to detect the role of FENDRR in HPAEC pyroptosis. (biomedcentral.com)
Detection5
  • Multiplex assays are the simultaneous detection and measurement of multiple analytes within a single sample. (polarismarketresearch.com)
  • The global multiplex assays market has grown significantly due to the rising demand for personalized medicine, and companion diagnostics has fueled the need for multiplex assays, as they allow for the simultaneous detection of multiple biomarkers associated with a specific disease or condition. (polarismarketresearch.com)
  • Furthermore, the experience gained from developing COVID-19 multiplex assays has paved the way for future applications in infectious disease diagnostics, potentially advancing syndromic testing and comprehensive pathogen detection. (polarismarketresearch.com)
  • The global burden of diseases such as cancer and the ongoing COVID-19 pandemic has further accelerated the need for multiplex assays for accurate and rapid pathogen detection. (polarismarketresearch.com)
  • Most recently, GeneXpert MTB/RIF (Cepheid) advanced nucleic acid-based detection by integrating PCR and a highly sensitive molecular-beacon assay into a single, automated system. (cdc.gov)
Results1
  • The pandemic has also emphasized the importance of point-of-care testing, where multiplex assays provide rapid results and aid in timely decision-making. (polarismarketresearch.com)
Fluorescent2
  • The Celigo is a bench-top, high throughput, micro-well plate based, bright field and fluorescent imaging system. (utsouthwestern.edu)
  • It consists of 4 fluorescent channels (UV to far red) and has extensive applications including bright field cell counting, wound-healing and cell growth assays, cell line development, induced pluripotent stem cell (iPSC) reprogramming, 3D invasion and migration modeling, immune-oncology, virology, and fluorescent-based assays. (utsouthwestern.edu)
Peripheral blood2
  • Peter Krutzik, Ph.D., a senior scientist in Gary Nolan's group at the Baxter Lab in genetics, pharmacology, microbiology, and immunology at Stanford University, described the use of phosphor flow cytometry to profile perturbations in signaling networks when peripheral blood cells are exposed to drug compounds. (genengnews.com)
  • Dr. Kritzik and colleagues are using phosphor-flow cytometry to study three phosphoproteins-all linked to disease and all potential drug targets-in subsets of peripheral blood cells. (genengnews.com)
Western2
  • The effects of PTX-induced exosomal circBACH1 on BC cell function were assessed using RNA Binding Protein Immunoprecipitation (RIP), dual luciferase reporter gene, tube formation, CCK-8, and Western Blot assays. (biomedcentral.com)
  • Cell surface EMMPRIN expression was measured by flow cytometry and Western blotting, and phospho-(p)-p38, p-JNK, nuclear NFκB p65, and phospho-p65 were measured by Western blotting. (biomedcentral.com)
Characteristics1
  • Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. (wikipedia.org)
Reporter2
  • The underlying mechanisms of action of LIPH-4 were explored through bioinformatics, luciferase reporter assay, RNA-immunoprecipitation assay and immunoblot. (researchsquare.com)
  • The gene promoter activity was determined by dul-luciferase reporter assay. (bvsalud.org)
Migration1
Experiments2
  • On the basis of the experiments we performed, we can conclude there is no evident difference except secreted Aβ 1-40 levels in phenotype between fAD and sAD samples. (biomedcentral.com)
  • The Cellomics machine allows you to not only image live cells, but to keep those cells alive for both short- and long-term experiments in a controlled environment similar to that of a tissue culture incubator. (lu.se)
Efficiency1
  • Analyzing various targets in a single assay provides several advantages, such as increased efficiency, cost-effectiveness, and reduced sample requirements. (polarismarketresearch.com)
Expression2
Cells6
  • As a postdoc in Manoj Duraisingh's lab at Harvard School of Public Health she performed a genetic screen to identify critical host factors for Plasmodium falciparum malaria using red blood cells derived from hematopoietic stem cells. (stanford.edu)
  • In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. (wikipedia.org)
  • The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. (wikipedia.org)
  • Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties. (wikipedia.org)
  • The flow cell has a liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing. (wikipedia.org)
  • Biological assays showed that invasive and migratory behavior was strengthened by hypoxia only in GBM stem cells. (biomedcentral.com)
Main1
  • The main objective of the core is to provide standardized immune-based assays, to develop, optimize, and validate new technologies, and to support efforts in the areas of human and animal immunology and immunotherapy. (augusta.edu)
Significantly1
  • The COVID-19 pandemic has significantly impacted the multiplex assays market , as these assays have played a crucial role in diagnosing and monitoring the virus. (polarismarketresearch.com)
Basic1
Applications1
  • Innovations like bead-based assays, microarrays, and next-generation sequencing have expanded the applications of multiplex assays. (polarismarketresearch.com)
Technology1
  • The advancements in technology and assay platforms have greatly improved the performance and capabilities of multiplex assays. (polarismarketresearch.com)
Instruments1
  • The market has experienced growth and opportunities, with a heightened need for assay kits, instruments, and consumables. (polarismarketresearch.com)
Data2
  • Dr. Robinson also discussed how data-extraction issues have historically been a stumbling block in the use of flow cytometry in drug discovery. (genengnews.com)
  • Taking advantage of the capacity for multiplexing, they can perform 14 assays per well, generating reproducible data with low intra- and inter-run variance. (genengnews.com)
Scientists1
  • The Cellomics Arrayscan VTI instrument is an imaging tool that will help our scientists make quicker and more precise analyses, capturing, for instance, changes in cell size, shape and intensity. (lu.se)
Technologies2
  • Equipment in this laboratory includes an Attune NxT Flow Cytometer from Life Technologies and a Sony SH800 Cell Sorter. (utsouthwestern.edu)
  • This surge in demand has driven research and development efforts, resulting in the introduction of new assay platforms and technologies. (polarismarketresearch.com)
Software2
  • Acquisition is mediated by a computer physically connected to the flow cytometer, and the software which handles the digital interface with the cytometer. (wikipedia.org)
  • The Flow Cytometry Core Facility is a shared resource providing access to flow cytometers, support equipment, and associated software and services. (augusta.edu)
Activity1
  • Lastly, proteasome activity was investigated using a fluorometric assay. (biomedcentral.com)
Ability to perform1
Development1
  • Additionally, research and development initiatives have played a vital role in advancing the multiplex assays market. (polarismarketresearch.com)
Test1
  • Gen-Probe RNA test, interferon-gamma assay, urine-based antigen test). (cdc.gov)
High4
  • The initial resistance that the high-content/high-throughput community had to face has been overcome, and now most in the field acknowledge that flow cytometry is enormously high throughput and very high content. (genengnews.com)
  • The increasing recognition and acceptance of molecular diagnostics, with their high sensitivity and ability to detect genetic variations, have further propelled the adoption of multiplex assays in personalized medicine. (polarismarketresearch.com)
  • The first label-free high-frequency impedance flow cytometer based on a patented microfluidic "lab-on-chip", Ampha Z30, was introduced by Amphasys (2012). (wikipedia.org)
  • Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput, and low-cost platform for point-of-care diagnostics. (cdc.gov)