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  • spindle
  • Aurora A is involved in mitotic entry, separation of centriole pairs, accurate bipolar spindle assembly, alignment of metaphase chromosomes and completion of cytokinesis. (imtm.cz)
  • Indirect immunofluorescence showed that Aurora B and EB1 colocalize during anaphase on the central spindle and in the midbody during cytokinesis. (wikipedia.org)
  • actin
  • Examination of preparations stained for tubulin, anillin, KLP3A, and F-actin revealed discrete defects in the components of the cytokinetic apparatus, suggesting that these genes act at four major points in a stepwise pathway for cytokinesis. (washington.edu)
  • Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. (wikipedia.org)
  • pathway
  • Cytokinesis failure triggers hippo tumor suppressor pathway activation. (nih.gov)
  • Here, we combine RNAi screening and in vitro evolution approaches to demonstrate that cytokinesis failure activates the Hippo tumor suppressor pathway in cultured cells, as well as in naturally occurring tetraploid cells in vivo. (nih.gov)
  • molecular
  • Yet, the identity of proteins required for the spatial and temporal regulation of molecular events during cytokinesis remains largely an open question. (rupress.org)
  • With this combination of genetics and cell imaging and modeling, a quantitative framework in which molecular mechanisms are related to cytokinesis dynamics becomes possible. (pnas.org)
  • mammalian
  • To investigate the involvement of centrioles in cytokinesis, we monitored the movements of centrioles in three mammalian epithelial cell lines, HeLa, MCF 10A, and the p53-deficient mouse mammary tumor cell line KP-7.7, by time-lapse imaging. (pubmedcentralcanada.ca)
  • segregation
  • Cytokinesis is the process that separates two daughter cells after chromosome segregation. (biologists.org)
  • Genetic analyses in simple model systems (yeast, slime mold, fruit fly) have demonstrated that defects in either karyokinesis, or nuclear division (e.g., mutations that affect cell cycle checkpoints, mitotic chromosome condensation and segregation, etc.), or cytokinesis ( Fig. 1 ), both may delay or block development. (rupress.org)
  • Without this regulation, signaling faults in cytokinesis can occur, resulting in unequal chromosome segregation or polyploid cells, greatly increasing the risk of cancer. (wikipedia.org)
  • proceeds
  • We find that cytokinesis proceeds through a single sequence of shape changes, but the kinetics of the transformation from one shape to another differs dramatically between strains. (pnas.org)
  • In all cell types, cytokinesis proceeds through essentially the same sequence of shapes. (pnas.org)
  • cells
  • A Subset of the BRCA2 +/− cells had delayed cytokinesis (40 min or longer) making the mean cell division time 6 min longer compared with BRCA2 +/+ cells, 33 min versus 27 min, respectively. (hindawi.com)
  • Our observations indicate that in a subset of cells the presence of only one wild type BRCA2 allele is insufficient for efficient cytokinesis. (hindawi.com)
  • Early studies of cytokinesis in animal cells took advantage of the ability to easily manipulate and observe large transparent eggs of marine invertebrates, such as echinoderms and ctenophores (reviewed by Rappaport 1996 ). (rupress.org)
  • Plant cells divide through the process of mitosis, followed by cytokinesis. (reference.com)
  • Using time-lapse imaging of HeLa cells, the authors show that depletion of BRCA2 abrogates its function in DNA repair but has no effect on cytokinesis. (biologists.org)
  • Furthermore, DLD1 colon cancer cells in which both BRCA2 alleles are disrupted successfully complete cytokinesis. (biologists.org)
  • In a new paper appearing in Science (10 October 2014, Science 346 (6206):244-247 ), Phuong Nguyen and Aaron Groen, along with their colleagues at Harvard Medical School (Boston, MA), have reconstituted the organization of cytokinesis signaling outside living cells, using a the cell-free Xenopus system. (xenbase.org)
  • Plant cells do not perform cytokinesis through this exact method but the two procedures are not totally different. (wikipedia.org)
  • BRCA2
  • We demonstrate that unmodified primary human fibroblasts derived from heterozygous BRCA2 mutation carriers show significantly prolonged cytokinesis. (hindawi.com)
  • In addition, a diffusion-based assay that precisely discerns the timing of abscission and cell separation shows that BRCA2 does not have a role in these final stages of cytokinesis. (biologists.org)
  • furrow
  • From the analysis, wild-type cytokinesis appears to proceed through an active, extremely regulated process in which globally distributed proteins generate resistive forces that slow the rate of furrow ingression. (pnas.org)
  • genes
  • These include 16 novel loci and three genes, diaphanous, four wheel drive, and pebble, already known to be involved in Drosophila cytokinesis. (washington.edu)
  • To define the primary defects leading to failure of cytokinesis, we analyzed meiotic divisions in male mutants for each of these 19 genes. (washington.edu)
  • membrane
  • Other enzymes or organelles such as cilia can be anchored to this scaffolding in order to control the deformation of the external cell membrane, which allows endocytosis and cytokinesis. (wikipedia.org)
  • largely
  • Cytokinesis largely resembles the prokaryotic process of binary fission, but because of differences between prokaryotic and eukaryotic cell structures and functions, the mechanisms differ. (wikipedia.org)