• Peripheral blood mononuclear cells (PBMCs) were isolated from the blood of 83 adult CF patients in stable clinical condition. (nih.gov)
  • Pre-incubation of PBMCs with IVIG inhibited lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA)/ionomycin stimulated cytokine secretion. (tmu.edu.tw)
  • We hypothesized that this poor response is associated with a reduced effect of corticosteroids to inhibit cytokine release from activated peripheral blood mononuclear cells (PBMCs). (nih.gov)
  • We recently demonstrated that autistic children and their mothers share high expression levels of some HERVs and cytokines in peripheral blood mononuclear cells (PBMCs) ex vivo, suggesting a close mother-child association in Autism Spectrum Disorder (ASD). (biomedcentral.com)
  • The results show that HERVs and cytokines expression can be modulated in vitro by different stimuli in PBMCs from autistic children and their mothers, while no significant changes were found in PBMCs ASD fathers or in controls individuals. (biomedcentral.com)
  • We also found, in separate studies, that microRNA (miRNA) expression in PBMo and mitochondrial respiration in peripheral blood mononuclear cells (PBMCs) differed in the IL-1ß/IL-10-based ASD subgroups. (frontiersin.org)
  • Serum miRNA levels were examined by high-throughput deep sequencing in ASD and non-ASD control sera with concurrent measurement of PBMo cytokine production and mitochondrial respiration by PBMCs. (frontiersin.org)
  • The objective of this study is to explore the long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) expression profiles of monocyte-derived dendritic cells (DCs) obtained from peripheral blood mononuclear cells (PBMCs). (frontiersin.org)
  • The peripheral blood mononuclear cells (PBMCs) have a round nucleus ( Delves, 2016 ). (frontiersin.org)
  • PBMCs are divided into various functional subtypes with respect to the specific cytokine expression profiles, surface markers, and the transcription factors. (frontiersin.org)
  • Objective To investigate the expression of Toll-like receptor 2 and 4 (TLR2 and TLR4) in peripheral blood mononuclear cells (PBMCs) and the serum inflammatory cytokine levels in patients with liver failure. (wanfangdata.com.cn)
  • To examine the activation of classic and 11-oxygenated androgens within human peripheral blood mononuclear cells (PBMCs), PBMCs were isolated from healthy donors and subsequent activity analysis assays were carried out. (bham.ac.uk)
  • Overall, this project has revealed that 11-oxygenated androgens are the favoured substrate for AKR1C3 in PBMCs, predominantly due to natural killer cell AKR1C3 activity, producing the major active androgen 11-ketotestosterone. (bham.ac.uk)
  • We investigated the mechanisms responsible for the regulatory effects of IVIG on in vitro human peripheral blood mononuclear cell (PBMC) cytokine production. (tmu.edu.tw)
  • The objective of the present study was the evaluation of cytokine patterns in terms of TNF-α, IL-10, IL-6, and IL-1β secretion in peripheral blood mononuclear cell (PBMC) supernatants isolated from blood of children affected by generalized epilepsy and treated in vitro with myofibrillar, sarcoplasmic, and total protein fractions of meat and fish sources. (unifg.it)
  • The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. (wikipedia.org)
  • This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. (wikipedia.org)
  • In particular, in vitro exposure to interleukin-2/Phytohaemagglutinin or valproic acid induces the expression of several HERVs and cytokines while Efavirenz inhibits them. (biomedcentral.com)
  • Three cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 3 (IL-3), and interleukin 5 (IL-5) have been shown to promote the growth and the maturation of eosinophils and to induce the conversion of normal eosinophils to hypodense eosinophils. (medscape.com)
  • Tissue explants were treated with proinflammatory cytokines relevant to RA pathogenesis (interleukin 1β (IL-1β), tumour necrosis factor (TNF), interferon γ, IL-15, IL-17, IL-23). (bmj.com)
  • Increased production of mediators, such as interleukin (IL)‐8, tumour necrosis factor‐α (TNF‐α) and leukotriene B 4 , which both attract inflammatory cells and increase oxidant production by these cells, has been found. (ersjournals.com)
  • OBJECTIVES Assessment of the numbers and spatial distribution of cells producing interleukin 1α (IL1α), interleukin 1β (IL1β), tumour necrosis factor α (TNFα), and interleukin 6 (IL6) in the synovial membranes of patients with rheumatoid arthritis (RA). (bmj.com)
  • An excessive extracellular presence of the proinflammatory cytokines interleukin 1 (IL1), tumour necrosis factor α (TNFα), and interleukin 6 (IL6), in particular, has been identified and intra-articular as well as systemic levels of these cytokines may to some extent reflect disease activity. (bmj.com)
  • Contrary to NGF, proNGF administration increased inflammatory cytokines but not interleukin (IL)-10 expression, inducing a stronger activation of p38 and JNK pathways. (bmj.com)
  • Furthermore, the proliferation of Th17 cells and Th17‑associated cytokines, including interleukin (IL)17, IL23 and RAR‑related orphan receptor γt, was analyzed by reverse transcription‑quantitative polymerase chain reaction. (spandidos-publications.com)
  • Interleukin (IL)17-producing Th17 cells, which are different from Th1 and Th2 cells, have been described as serving critical roles in inflammation and autoimmune diseases, as well as in cancer development ( 8 - 11 ). (spandidos-publications.com)
  • RESULTS: DE increased lung-associated CD3+CD4+ T cells and interleukin (IL)-17 (but not IL-4, interferon [IFN]-y, IL-10) producing CD4+ T cells. (cdc.gov)
  • Synthesis and release of interleukin 1 by reoxygenated human mononuclear phagocytes. (jci.org)
  • This gene, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. (antibodies-online.com)
  • These clones then release proinflammatory cytokines, such as tumor necrosis factor-alpha, interleukin-2, and interferon -gamma. (msdmanuals.com)
  • Previously, we have shown in a mouse model that exposure to hog dust extract (HDE) collected from a CAFO results in the activation of protein kinase C (PKC), elevated lavage fluid cytokines/chemokines including interleukin-6 (IL-6), and the development of significant lung pathology. (cdc.gov)
  • Additionally, alcohol inhibits the lipopolysaccharide (LPS)-induced inflammatory cytokine, interleukin-6 (IL-6), via the p38 ERK1/2 MAPK pathway [ 9 ]. (cdc.gov)
  • Remarkably, the antiretroviral drug Efavirenz restores the expression of specific HERV families to values similar to those of the controls, also reducing the expression of proinflammatory cytokines but keeping the regulatory ones high. (biomedcentral.com)
  • Proinflammatory cytokines (TNF-α, MIP-1, i-NOS) were decreased significantly and restoration of tissue damage was observed in all organs. (nature.com)
  • We investigated the initial levels of proinflammatory cytokines and acute phase reactants (APR), e.g. (karger.com)
  • The aim of the present study was to investigate whether CRH may affect cytokine production and activity in human peripheral blood mononuclear cells (PBMC). (unisi.it)
  • Cytokine gene expressions were again monitored by RT-qPCR after PBMC treatment with GAL3, with or without YKL-40 co-stimulation. (nih.gov)
  • Chemokine/cytokine responses in immune complex triggered IFN-α activity was studied in healthy donor peripheral blood mononuclear cells (PBMC). (lu.se)
  • RNAseq analysis of FACS-sorted PBMC subpopulations (dice-database.org) revealed natural killer cells to be the major location of AKR1C3 activity and expression. (bham.ac.uk)
  • Our data also showed that cross-linking cell membrane-bound IVIG with anti-human kappa- and lambda-chain antibodies resulted in cytokine secretion levels similar to those elicited by LPS. (tmu.edu.tw)
  • Unmethylated CpG dinucleotides in particular base contents that are relatively abundant in bacterial DNA are known to stimulate innate immune responses including immune cell proliferation and cytokine secretion in mammalian. (usda.gov)
  • Furthermore, all assays designed to quantify extracellular levels of cytokines will measure the net outcome of secretion, absorption to numerous cellular and soluble cytokine receptors, and degradation by proteases present at inflammatory sites. (bmj.com)
  • There is evidence that 2D or 3D culturing conditions have an impact on the cells' secretome, and here we aimed to compare the secretion of cytokines and growth factors in human MSCs from different sources cultured in 2D and 3D conditions and assess their effect on human macrophages polarization in vitro. (biomedcentral.com)
  • Gluten peptides enter the pancreas where they affect the morphology and might induce beta-cell stress by enhancing glucose- and palmitate-stimulated insulin secretion. (mdpi.com)
  • IL-37, a newly found anti-inflammatory cytokine of the IL-1 family, has both extracellular and intracellular functions. (nature.com)
  • IL-37: a new anti-inflammatory cytokine of the IL-1 family. (nature.com)
  • Our findings suggest that umbilical cord-derived MSCs' conditioned media demonstrated the highest cytokine and growth factor levels and despite mostly pro-inflammatory cytokine profile were able to promote anti-inflammatory macrophage polarization. (biomedcentral.com)
  • The autoimmune nature of diabetes and inflammatory cytokine IL-10 (as markers the major contribution of lymphocyte T- of inflammatory changes) and the soluble cells are well established. (who.int)
  • Regarding host factors, macrophages from DL patients tokines and chemokines in supernatants of mononuclear allow for greater parasite multiplication than cutane- cells stimulated with leishmania antigen. (cdc.gov)
  • Recently, adipose tissue has been shown to synthesise and release highly bioactive substances: classical adipokines (eg, leptin, adiponectin) and various proinflammatory factors such as cytokines, chemokines, growth factors and complement components. (bmj.com)
  • This study evaluates galectin-9 and an array of chemokines/cytokines in their potential as surrogate markers of type I IFN and/or SLE disease activity. (lu.se)
  • Levels of chemokines/cytokines and galectin-9 were measured by immunoassays. (lu.se)
  • Gene targets of the altered miRNAs in all the ASD subgroups were enriched in pathways of neuronal development and synaptic plasticity, along with cell proliferation/differentiation. (frontiersin.org)
  • Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells. (cancerindex.org)
  • Androgens are also key regulators of immune cell function and have a suppressive influence on differentiation, proliferation, and cytokine production. (bham.ac.uk)
  • We report an unusual case of Langerhans cell granulomatosis (LCG) manifested as a villous synovial proliferation in a 38-year-old female jogger. (medscape.com)
  • It emphasizes the importance of immunocytochemistry to separate this histiocyte-like cell proliferation from true histiocytic and other dendritic cell types. (medscape.com)
  • Shan J, Ma JM, Wang R, Liu QL, Fan Y. Proliferation and Apoptosis of Peripheral Blood Mononuclear Cells in Patients with Oral Lichen Planus. (medscape.com)
  • Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and is widely used as a tool for the establishment of B cell lines producing human monoclonal antibodies. (lu.se)
  • The inflammatory process in patients with stable COPD is dominated by macrophages, CD8+ T‐lymphocytes and neutrophils, and to a lesser extent mast cells, in the bronchial submucosa and alveoli 5 . (ersjournals.com)
  • CD4 and CD8 T lymphocytes play an import role in the inflammatory response, as these cells may manage the profile of cytokines produced against an infectious agent 4 . (bvsalud.org)
  • Most T cells in the epithelium and adjacent to the damaged basal keratinocytes are activated CD8 + lymphocytes. (medscape.com)
  • Activated CD8 + T cells (and possibly keratinocytes) may release cytokines that attract additional lymphocytes into the developing lesion. (medscape.com)
  • Methods Real-time PCR, western blot and ELISA were performed to evaluate NGF, proNGF, their receptor and cytokine expression in synovial tissue and cells of patients with juvenile idiopathic arthritis (JIA) and rheumatoid arthritis (RA), and controls. (bmj.com)
  • p75NTR, the specific receptor for proNGF, is the NGF receptor most expressed in mononuclear cells of patients with JIA, while TrkA is the prevalent receptor in healthy donors. (bmj.com)
  • T cell receptor (TCR) aß knockouts were used to determine the functional importance of aß-expressing T cells. (cdc.gov)
  • The receptor of this cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). (antibodies-online.com)
  • Aim: To evaluate the involvement of Th2 cells in different periods of the active phase of experimental periodontal disease and expression of the R1 subunit of the receptor for IFN-γ during the early and advanced progression of the disease. (bvsalud.org)
  • This signal together with the T Cell Receptor (TCR) induces the expression of GATA-3. (bvsalud.org)
  • Younes F, Quartey EL, Kiguwa S, Partridge M. Expression of TNF and the 55-kDa TNF receptor in epidermis, oral mucosa, lichen planus and squamous cell carcinoma. (medscape.com)
  • The present results also suggest that one potential role of the MP6-derived thioredoxin could be the up regulation of IL-6 receptor expression in EBV-infected B cells. (lu.se)
  • His interests then broadened to the impact of these oxidants on neighbouring cells during inflammation, and the controlled generation of oxidants by other cells during normal metabolism. (otago.ac.nz)
  • 1 In RA, numerous cytokines play a fundamental role in inflammation and joint destruction and are thought to originate primarily from synoviocytes and leucocytes that massively infiltrate synovial tissue. (bmj.com)
  • Anti-inflammatory cytokines such as IL-10 inhibit inflammation and enhance tissue healing. (nature.com)
  • RESULTS A wide variation between subjects was recorded for both quantities and profiles of expressed cytokines, despite similar macroscopic and histopathological features of inflammation. (bmj.com)
  • Specimens acquired arthroscopically from areas with maximum signs of macroscopic inflammation showed an increased number of TNFα producing cells in pannus tissue compared with that occurring in synovial villi of a given joint. (bmj.com)
  • Cytokines, locally produced in the synovial membrane of patients with rheumatoid arthritis (RA), play an important part in regulating the chronic joint inflammation which may lead to serious tissue damage, such as cartilage degradation, bone resorption, and fibrosis. (bmj.com)
  • MSCs are reported to contribute both to the inflammation initiation and to its resolution via complex cross talk with immune cells, most importantly macrophages [ 1 ]. (biomedcentral.com)
  • This paper has particularly focused on Th17 cells, which are called "17" since they produce the cytokine called IL-17, which recruits immune cells such as neutrophils to the site of inflammation and regulates these proinflammatory cells at the site of infection. (sciencewatch.com)
  • Th1 cells that produce IL-2, IFN- γ , TNF- α, and TNF- β evoke cell-mediated immunity and phagocyte-dependent inflammation while Th2 cells that produce IL-5, IL-4, IL-6, IL-10, and IL-13 induce a strong antibody response that comprises the IgE class and eosinophil accumulation, but it inhibits several functions of phagocytic cells [ 7 ]. (hindawi.com)
  • Recent studies on diseases which involve insulin insensitivity (e.g. obesity, type 2 diabetes and atherosclerosis) also show increased cytokine production and markers of inflammation. (lww.com)
  • Herein we show that autistic children and their mothers share an intrinsic responsiveness to in vitro microenvironmental changes in expressing HERVs and pro-inflammatory cytokines. (biomedcentral.com)
  • elicits antiproliferative and proapoptotic activities in vitro in solid and hematologic tumor cells. (medscape.com)
  • We determined the presence of EndoMT in the pulmonary vasculature in vivo and the functional effects on pulmonary artery endothelial cells (PAECs) undergoing EndoMT in vitro. (surrey.ac.uk)
  • [ 8 ] T cells in oral lichen planus contain mRNA for TNF and secrete TNF in vitro. (medscape.com)
  • Because alcohol blocks airway epithelial cell release of IL-6 in vitro , we hypothesized that alcohol exposure would alter mouse lung inflammatory responses to HDE. (cdc.gov)
  • The present study investigated the expression of T helper (Th)17 cytokines in patients with laryngeal squamous cell carcinoma (LSCC) and their clinical significance in providing new therapeutic insights. (spandidos-publications.com)
  • Conclusion Rheumatoid AAT is highly reactive tissue which upon stimulation secretes considerable amounts of proinflammatory (IL-6, IL-8, TNF) and anti-inflammatory (IL-1Ra) cytokines and classical adipokines. (bmj.com)
  • Here, we report a novel approach to treat septicemia associated with intra-abdominal infection in a murine model by delicately balancing pro- and anti-inflammatory cytokines. (nature.com)
  • We approached this issue with a small natural product derived oligosaccharide with TLR4 modulating activity that delicately balances the immune system and the production of anti-inflammatory and pro-inflammatory cytokines in a murine model of peritonitis. (nature.com)
  • Techniques based on immunohistochemistry have thus also been widely used to study the expression of cytokines within the synovial tissue in attempts to determine true local production. (bmj.com)
  • 5 , - , 9 This loose connective tissue is composed of extracellular matrix and various types of cells: fat cells-named adipocytes, and the non-fat cells of the stromal vascular fraction that contains preadipocytes, capillary endothelial cells, infiltrating leucocytes and multipotent stem cells. (bmj.com)
  • Intracellular mature form of IL-37, but not its extracellular form, markedly inhibited migration of multiple kinds of tumor cells through inhibiting Rac1 activation. (nature.com)
  • MSCs' secretome (i.e., cytokines, growth factors, and extracellular vesicles) is therefore increasingly discussed in the context of their ability to modulate inflammatory response and promote regeneration. (biomedcentral.com)
  • Cytokines are mediators that, among other functions, can cause bone resorption and dissolution of the extracellular matrix. (bvsalud.org)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Here, we have screened more than 20 CpG-ODN for their ability to stimulate nitric oxide (NO), IL-1beta, and IFN-gamma production using chicken macrophage cell HD-11 and peripheral blood mononuclear cells. (usda.gov)
  • Our results showed that synthetic oligodeoxynucleotides containing CpG motif were able to activate the avian macrophage cells and induce NO production. (usda.gov)
  • In Gram-negative bacterial sepsis, production of excess pro-inflammatory cytokines results in hyperinflammation and tissue injury. (nature.com)
  • 1-5 The paracrine or autocrine mode of action of most cytokines complicates the interpretation of studies of body fluids for reliable assessment of cytokine production. (bmj.com)
  • 6-16 However, most previous studies based on immunohistochemical staining of intra-articular cytokine production in RA have been performed on acetone fixed tissue, a procedure which does not readily allow subsequent discrimination of cytokine producing cells and cytokine binding cells. (bmj.com)
  • proNGF effects depend on its binding to p75NTR, as inhibition of p75NTR with neutralising antibodies or LM11A-31 abolished proNGF-induced production of IL-6 in patients' mononuclear cells, while inhibition of TrkA did not. (bmj.com)
  • Altered cytokine production can lead to immune dysfunction in patients with cancer. (spandidos-publications.com)
  • To examine the possible involvement of cytokines in reperfusion injury, we have studied production of IL-1 by human vascular cells, including smooth muscle and mononuclear phagocytes. (jci.org)
  • Dehoux MS, Boutten A, Ostinelli J, et al: Compartmentalized cytokine production within the human lung in unilateral pneumonia. (karger.com)
  • The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. (antibodies-online.com)
  • In this study production levels of interleukins (IL)-12 and IL-13 were measured by commercial ELISA in culture supernatants of mitogen-stimulated peripheral blood mononuclear cells from 30 non-splenectomized beta-thalassaemia cases with iron overload and 20 age- and sex-matched healthy individuals. (who.int)
  • Dans la présente étude, les taux de production des interleukines 12 et 13 (IL-12 et IL-13) ont été mesurés à l'aide de la méthode ELISA dans les surnageants de culture de cellules mononucléées de sang périphérique stimulées par des mitogènes chez 30 cas de bêta-thalassémie non splénectomisés présentant une surcharge martiale ainsi que chez 20 individus en bonne santé appariés pour l'âge et le sexe. (who.int)
  • Hypodense eosinophils are activated cells with increased survival and an increased capacity for cytotoxicity, and their release of inflammatory mediators, such as leukotrienes, is increased. (medscape.com)
  • Th1 cells are effectors of cell- sis on these mediators. (who.int)
  • Patients were ous leshmaniasis (CL) cells ( 14 ). (cdc.gov)
  • Histologic assessment of patients with systemic sclerosis-associated PAH and the hypoxia/SU5416 mouse model identified the presence von Willebrand factor/α-smooth muscle actin-positive endothelial cells in up to 5% of pulmonary vessels. (surrey.ac.uk)
  • Concordant results for the detection of TNFα at protein and mRNA levels were obtained with an unexpectedly low number of TNFα producing cells compared with IL1 expressing cells in many patients with RA. (bmj.com)
  • CONCLUSION The recorded heterogeneous profile of proinflammatory cytokine synthesis in the synovial membrane among patients with RA may provide a clue for an understanding of the wide variation in responsiveness to different modes of antirheumatic treatment between patients. (bmj.com)
  • The prevalence of Th17 cells and their receptors in patients with LSCC was studied using immunohistochemical analysis via tissue microarray technology. (spandidos-publications.com)
  • The results revealed that the prevalence of Th17 cells in patients with LSCC was elevated in their primary tumors, as well as in peripheral blood, compared with that in healthy controls. (spandidos-publications.com)
  • The present study revealed that patients with LSCC have elevated levels of Th17 cells in their primary tumors and peripheral blood compared with those in healthy controls. (spandidos-publications.com)
  • A heatmap showing increased levels of most measured cytokines in patients who had contracted coronavirus disease 2019 (COVID-19). (ajtmh.org)
  • Before the discovery that the major fibril component in these patients was an immunoglobulin fragment, patients with light chain-type amyloidosis were described as having primary (in the sense of idiopathic) amyloidosis or, when the burden of monoclonal plasma cells was large, myeloma-associated amyloidosis. (medscape.com)
  • In most patients with a monoclonal plasma cell disorder, whether multiple myeloma or MGUS, the monoclonal L chain secreted by the clone remains soluble in the bloodstream. (medscape.com)
  • The role of autoimmunity in the pathogenesis is supported by many autoimmune features of oral lichen planus, including its chronicity, onset in adults, predilection for females, association with other autoimmune diseases, occasional tissue-type associations, depressed immune suppressor activity in patients with oral lichen planus, and the presence of autocytotoxic T-cell clones in lichen planus lesions. (medscape.com)
  • ABSTRACT This study assessed changes in serum levels of cytokines IFN and IL-10 (biomarkers of in ammatory changes) and soluble biomarkers sICAM-1 and sE-selectin (biomarkers of endothe- lial dysfunction) in diabetic patients with and without nephropathy. (who.int)
  • Les IFN et les IL-10 étaient signi cativement élevés chez ceux qui présentaient une néphropathie diabétique (ND) et une maladie rénale en phase terminale (MRPT) par rapport aux témoins et aux patients diabétiques sans ND. (who.int)
  • T-cells have 2 subsets of betic patients with and without nephropathy. (who.int)
  • Th2 cells produce cy- patients which might reduce the morbidity tokine interleukins IL-4 and IL-10, which and mortality due to infection. (who.int)
  • ASD subgroup-specific associations were observed between serum miRNA expression and IL-1ß/IL-10 ratios, mitochondrial respiration, and monocyte cytokine profiles (IL-10, CCL2, and TNF-α). (frontiersin.org)
  • Our results show that the murine thymoma cell line EL-4 is superior to peripheral blood mononuclear cells in both cellular outgrowth and cloning experiments, whereas monocyte-derived factors and monocyte cell lines were less capable than peripheral blood mononuclear cells in enhancing cellular outgrowth and cloning. (lu.se)
  • Our main goal was to evaluate if YKL-40 can modulate the expression of major cytokines (IL-6, IL-10, IL-13) implicated in the inflammatory response in CF. A secondary goal was to explore the interactions between YKL-40 and other circulating proteins to determine the impacts on cytokine modulation. (nih.gov)
  • We also found that CpG-ODN differentially stimulated expression of cytokine genes. (usda.gov)
  • The optimal CpG motif for NO induction was also a strong stimulant for the IL-1beta gene expression in the macrophage HD-11 cells, whereas different CpG motifs have found to induce the IFN-gamma gene expression in PMNC. (usda.gov)
  • Conversely, no differences were observed pertaining to the expression to regulatory cytokines IL-4, IL-10, IL-13, TSG-6, or TGF-β. (deepdyve.com)
  • Both DE and PGN augmented expression of cytokines associated with Th1 and Th17 polarization in lung homogenates. (cdc.gov)
  • Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. (cancerindex.org)
  • The analysis indicated a shift in the expression of the cytokines after treatment with all the cytokines being downregulated, except TNF. (hindawi.com)
  • There was a general trend of decrease in the expression of the cytokines at six and twelve weeks after treatment as compared to the pretreatment levels. (hindawi.com)
  • 0.05 indicates that leprosy significantly influenced the levels of cytokine expression. (ajtmh.org)
  • The pathogenesis of the disease is unclear, but altered expression of cytokines and cellular adhesion molecules, important for chemotaxis of LC, are thought to be involved in its development. (medscape.com)
  • Results: Light microscopy analysis revealed a decreased expression of GATA-3-positive cells when bone loss progressed. (bvsalud.org)
  • IFN-γ R1 was detected at an early stage during the active phase of disease, but the expression of positive cells remained unaltered during the remaining period of the study. (bvsalud.org)
  • The protein encoded by this gene is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. (antibodies-online.com)
  • Langerhans cell granulomatosis (LCG) is a clonal disorder characterized by lesions containing histiocyte-like cells, giant cells, and eosinophils. (medscape.com)
  • Recent studies suggest that the clonal expansion of EBV-infected T or natural killer cells plays a crucial role in the pathogenesis of chronic active EBV infection. (researchgate.net)
  • Clonal plasma cell proliferative diseases in which the Vl 6 gene is expressed are always associated with amyloid deposition. (medscape.com)
  • A modified immunohistochemical method detecting cytokine producing rather than cytokine binding cells was applied to determine cytokine synthesis in fixed cryopreserved sections. (bmj.com)
  • Immunoglobulin-related amyloidosis is a monoclonal plasma cell disorder in which the secreted monoclonal immunoglobulin protein forms insoluble fibrillar deposits in one or more organs. (medscape.com)
  • In contrast, the processed bovine, porcine and equine samples did not induce significant activation of cells of the innate immune system. (biomedcentral.com)
  • Gao W, Kumar S, Lotze MT, Hanning C, Robbins PD, Gambotto A . Innate immunity mediated by the cytokine IL-1 homologue 4 (IL-1H4/IL-1F7) induces IL-12-dependent adaptive and profound antitumor immunity. (nature.com)
  • these 2 events, in turn, can inhibit growth of neoplastic cells. (medscape.com)
  • Thus, new therapeutic approaches are needed to effectively inhibit the hyperinflammation produced by the overwhelming cytokine response during bacterial infection without developing resistance to secondary infection in abdominal infections like peritonitis. (nature.com)
  • There is a growing body of evidence that multipotent mesenchymal stromal cells' (MSCs') remarkable therapeutic potential is attributed not only to their differentiation and regenerative capacity, but also to the paracrine effect, underlying their immunomodulatory properties. (biomedcentral.com)
  • Cluster of differentiation (CD) 4 + T cells are essential organizers of cell-mediated immunity, participating in each stage of the immune response. (spandidos-publications.com)
  • Differentiation into Th1 depends on the presence of IFN-γ and IL-12, which bind to receptors on the surface of CD4 T cells 5 . (bvsalud.org)
  • This binding initiates a cascade of events that culminates in differentiation into Th1 cells, by increasing the transcription factor T-bet 12 . (bvsalud.org)
  • Th2 cells have a differentiation process which is dependent on IL-4, which causes the activation of the transcription factor STAT 6 . (bvsalud.org)
  • Furthermore, the human T cell hybridoma cell line MP6 that secretes a B cell growth and differentiation factor, recently identified as an isoform of thioredoxin, is also capable of stimulating EBV-infected B cells and lymphoblastoid cell lines. (lu.se)
  • Data were classified according to three different levels of cytokines produced from the EC group relative to the CC group. (unifg.it)
  • on the contrary, IL-1β levels were found to be significantly affected by the tested proteins fractions, as well as different meat sources, in high-level cytokine group. (unifg.it)
  • High levels of chemokine ing that impairment in the T-cell response is the cause ligand 9 were associated with more severe DL. (cdc.gov)
  • The reduction in the cytokine levels can be directly related to the influence of the drug praziquantel, modulating the cytokine response by elimination of adult worms, decline in parasitic load, and reduction of morbidity. (hindawi.com)
  • Oral lichen planus lesions contain increased levels of the cytokine tumor necrosis factor (TNF)-alpha. (medscape.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • [ 3 , 4 ] Langerhans cells are stellate dendritic cells that arise from CD34-positive bone marrow stem cells [ 5 ] and are not a member of the mononuclear phagocytic system. (medscape.com)
  • Our results indicate that the CRP level is higher in the serum than in the BAL fluid in the lung, and that IL-6 is the most important cytokine for the determination of the severity of the disease. (karger.com)
  • The objective of this study was to evaluate the cytokine profiles of IL-4, IL-2, IL-10, IL-5, IFN- γ, and TNF in serum samples of infected school-aged children by using flow cytometry before and after treatment. (hindawi.com)
  • It was further demonstrated that Th17 cells could be induced and expanded in the tumor microenvironment through cytokines produced by the tumor cells. (spandidos-publications.com)
  • To date, three broad approaches to adjunctive (non-antibiotic) therapy have been considered for the treatment of sepsis: (1) optimizing oxygen delivery through oxygenation/ventilation strategies and fluid/vasopressor use to maintain peripheral perfusion, (2) reducing bacterial virulence factors via anti-endotoxin antibodies and endotoxin removal columns and (3) targeting host response factors using corticosteroids, anti-cytokine drugs, and anticoagulants. (nature.com)
  • The proteasome pathway is an enzyme complex existing in all cells, which degrades ubiquitinated proteins that control the cell cycle and cellular processes and maintains cellular homeostasis. (medscape.com)
  • The cellular response pattern is associated with both the acute and chronic phases of the disease, in which cytokines play a critical role. (hindawi.com)
  • The cellular makeup of these monocytoid/histocytic cells is heterogeneous and ranges from cells of lymphoid, histiocytic, and dendritic origin. (medscape.com)
  • We have here examined various cell-derived cytokines for their ability to enhance both the cellular outgrowth of newly infected B cells and the clonability of infected B cells and lymphoblastoid cell lines. (lu.se)
  • Moreover, we uncovered a novel mechanism by which IL-37 suppressed tumor cell migration via its intracellular mature form (amino acids 46-218). (nature.com)
  • Antigens present on cell surface membranes and extracellularly in the tissue specimens are generally accurately shown after acetone fixation, while the intracellular morphology is poorly preserved. (bmj.com)
  • Enumeration of T cells and intracellular cytokine profiles were conducted by flow cytometry techniques. (cdc.gov)
  • Dendritic cells (DCs), which are the most important antigen-presenting cells (APCs) that send signals to the T cells, mainly participate in the pathogenesis of many diseases with immunoregulatory mechanisms, such as AR. (frontiersin.org)
  • Considering these facts, it could be concluded that pro-inflammatory Th17 cells may have extensive effects on LSCC pathogenesis and anti-tumor response. (spandidos-publications.com)
  • This cell-mediated immune response is ultimately responsible for the widespread tissue injury, in addition to the fibrosis of the skin and the connective tissue that pervades muscles, nerves, and other organs. (medscape.com)
  • Therefore, cytokine response is directly related with the influence of treatment in the variation of the immune response. (hindawi.com)
  • Th1 and Th2 responses are crucial in understanding the role of cytokines in the immune response to infectious agents [ 4 , 5 ]. (hindawi.com)
  • 8 ] speculated that IL-10 is important in regulating the human immune response to Schistosoma mansoni infection and that it is the main cytokine that plays part in the control of morbidity. (hindawi.com)
  • These cytokines amplify the immune response, resulting in formation of mononuclear cell infiltrates and noncaseating granulomas in target organs where beryllium has deposited. (msdmanuals.com)
  • Pulmonary arterial hypertension (PAH) is a progressive disease characterized by lung endothelial cell dysfunction and vascular remodeling. (surrey.ac.uk)
  • During embryogenesis endothelial cells exhibit substantial plasticity that contribute to cardiac development by undergoing endothelial-to-mesenchymal transition (EndoMT). (surrey.ac.uk)
  • Induced EndoMT cells exhibited up-regulation of mesenchymal markers, including collagen type I and α-smooth muscle actin, and a reduction in endothelial cell and junctional proteins, including von Willebrand factor, CD31, occludin, and vascular endothelial-cadherin. (surrey.ac.uk)
  • IL1α was predominantly seen in vascular endothelial cells, whereas IL1β staining was mainly shown in macrophages and fibroblasts. (bmj.com)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • Diabetic nephropathy (DN) is a leading selectin), and soluble thrombomodulin--are cause of chronic renal failure and is a grow- providing further evidence of the relation- ing concern given the increasing incidence ship between endothelial cell activation and of type 2 diabetes. (who.int)
  • found evidence and they did not require insulin therapy of endothelial cell injury in renal failure for glucose control. (who.int)
  • They can act as signalling molecules that regulate a number of life and death pathways in cells, and they are generated by the immune system to fight microbes. (otago.ac.nz)
  • Progression of periodontal lesions is caused by dysregulation of molecules (cytokines) released by specific cell populations 5 . (bvsalud.org)
  • Beryllium is presented to CD4 + T cells by antigen-presenting cells, principally in HLA-DP molecules. (msdmanuals.com)
  • This cytokine, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. (antibodies-online.com)
  • In conclusion, Th17 cells have a substantial impact on the carcinogenesis of LSCCs, and could serve as a potential therapeutic target to modulate the anti‑tumor response in these carcinomas. (spandidos-publications.com)
  • ABSTRACT The role of inflammatory cytokines in the pathophysiology of beta-thalassaemia is still unclear. (who.int)
  • CD40 ligand (CD40L) is mainly expressed on activated platelets and CD4+T cells, and it can be cleaved from the cell surface, releasing a soluble CD40L (sCD40L). (researchgate.net)
  • In fact, examination of muscle biopsy specimens reveals a dramatic inflammatory infiltrate that is predominantly composed of mononuclear cells and activated T cells. (medscape.com)
  • I was working on the fungal pathogen Candida albicans and wanted to identify the components of its cell wall, and the receptors of the human host response that were important for the development of Candida -specific Th17 responses in humans. (sciencewatch.com)
  • Langerhans cell granulomatosis cells are abnormal and coexpress S100 and CD1a, and these markers are used to separate this subpopulation of dendritic cells from other histiocyte-like cells. (medscape.com)
  • CONCLUSION: Studies demonstrated that DE and PGN exposure promote a Th1/Th17 lung microenvironment and that aß-expressing T cells are important in mediating DE-induced lung pathologic conditions. (cdc.gov)
  • Suppression of antigen-specific adaptive immunity by IL-37 via induction of tolerogenic dendritic cells. (nature.com)
  • Our adaptive immunity, also called acquired immunity, is composed of lymphocyte cells that are triggered when a specific pathogen enters the body. (squareonepublishers.com)
  • Tests such as ELISpot enumerate cells releasing IFN-g after mononuclear cells recovered from whole blood are incubated with similar antigens. (cdc.gov)
  • It has been confirmed that naïve (uncommitted) CD4 + T cells can be induced to different specific lineages according to the local cytokine, including towards T helper (Th)1, Th2, Th17 and T regulatory (Treg) cells ( 7 ). (spandidos-publications.com)
  • To identify new key elements that are important for this development, this study has elucidated the parts of the immune system that are important for the development of potent proinflammatory responses induced by cells of the immune system such as T helper cells. (sciencewatch.com)
  • T helper (Th) cells can be divided into Th1, Th2, and Th17. (sciencewatch.com)
  • Circulating plasma inflammatory and regulatory cytokines were assessed with enzyme-linked immunosorbent assays. (deepdyve.com)
  • OBJECTIVE: Because mice repetitively exposed to organic dust extracts (DE) display increased CD3+ T cell lung infiltrates, we sought to determine the phenotype and importance of these cells. (cdc.gov)
  • In particular, either cytokines activate the hypothalamus-pituitary-adrenal axis (HPA) or corticotropin-releasing hormone (CRH) induces the release of beta-endorphin from peripheral human mononuclear cells. (unisi.it)
  • The present results suggest a role for CRH as a paracrine mediator for human immune cells, increasing the evidence of a clear correlation between immune and neuroendocrine system. (unisi.it)
  • Prof Hampton's research is focused on how understanding how cells sense and respond to oxidants, and how these pathways might be modulated to prevent and treat human disease. (otago.ac.nz)
  • Human peripheral blood mononuclear cells-derived macrophages were then treated with umbilical cord-derived MSCs' conditioned media and their effect on macrophages polarization was assessed. (biomedcentral.com)
  • There was no difference in baseline spontaneous or stimulated release of these cytokines among groups. (nih.gov)
  • Results Spontaneous cytokine release from AAT was ≤12% of that produced by SM, while leptin was secreted in similar amounts. (bmj.com)