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CytokinesSuppressor of Cytokine Signaling ProteinsReceptors, CytokineTumor Necrosis Factor-alphaInterleukin-6Interferon-gammaInterleukin-10Cells, CulturedMice, Inbred C57BLInterleukin-4Th2 CellsInterleukinsTh1 CellsRNA, MessengerSignal TransductionLipopolysaccharidesCytokine Receptor gp130InflammationMice, Inbred BALB CInterleukin-12Lymphocyte ActivationMice, KnockoutInterleukin-8Inflammation MediatorsMacrophagesT-LymphocytesInterleukin-2Leukocytes, MononuclearInterleukin-1betaGene Expression RegulationCD4-Positive T-LymphocytesMonocytesFlow CytometryEnzyme-Linked Immunosorbent AssayCell LineNF-kappa BChemokinesDendritic CellsDisease Models, AnimalInterleukin-5Reverse Transcriptase Polymerase Chain ReactionGene ExpressionSpleenInterleukin-13Up-RegulationImmunity, InnateReceptors, InterleukinInterleukin-17Interleukin-18Granulocyte-Macrophage Colony-Stimulating FactorAntigens, CDT-Lymphocyte SubsetsRecombinant ProteinsCell DifferentiationInterleukin 1 Receptor Antagonist ProteinToll-Like Receptor 4CD8-Positive T-LymphocytesToll-Like ReceptorsLungMice, TransgenicAdjuvants, ImmunologicMembrane GlycoproteinsReceptors, Interleukin-6Molecular Sequence DataTransforming Growth Factor betaToll-Like Receptor 2Time FactorsInterleukin-15Killer Cells, NaturalCell ProliferationDown-RegulationReceptors, Interleukin-1T-Lymphocytes, Helper-InducerMacrophages, PeritonealCoculture TechniquesApoptosisMacrophage ActivationCell DivisionSTAT1 Transcription FactorChemokine CCL2ImmunophenotypingReceptors, Tumor Necrosis FactorInterleukin-12 Subunit p40NeutrophilsMice, Inbred C3HDNA-Binding ProteinsPhosphorylationBronchoalveolar Lavage FluidImmunologic FactorsTrans-ActivatorsBase SequenceAnti-Inflammatory AgentsOvalbuminAntigens, CD3Dose-Response Relationship, DrugImmunity, CellularOncostatin MImmunoglobulin EBlotting, WesternEpithelial Cells
SynthesisInterleukinInflammatory cytokinesMacrophage migration inhibitory factorsHuman peripheral blood mononuclearFactorAnti-inflammatoryStimulatorySecretionLymphocytesCellsMonocytesActivatesInterferon-gammaProductionInflammationReceptorsGDF15Monoclonal antibodyAntibodiesProliferationMoleculesSolubleProteinPathwaysMilieuMechanismsEosinophilic fasciitisProteinsCellMicrobialEffectsPhenotypeStaphylococcusCONCLUSIONBoneInteractionRole
Synthesis6
  • Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. (abcam.com)
  • IL-10 is also known as Cytokine Synthesis Inhibitory Factor (CSIF). (bdbiosciences.com)
  • IL-10 has numerous biological activities including the inhibition of cytokine synthesis by activated T cells, NK cells, monocytes, and macrophages. (bdbiosciences.com)
  • A modified immunohistochemical method detecting cytokine producing rather than cytokine binding cells was applied to determine cytokine synthesis in fixed cryopreserved sections. (bmj.com)
  • CONCLUSION The recorded heterogeneous profile of proinflammatory cytokine synthesis in the synovial membrane among patients with RA may provide a clue for an understanding of the wide variation in responsiveness to different modes of antirheumatic treatment between patients. (bmj.com)
  • matory response, IL-10 has been shown to inhibit macrophage and Chronic beryllium disease (CBD) provides a human disorder in T lymphocyte cytokine synthesis and MHC class II and B7 ex- which to study the delayed type hypersensitivity response to persistent pression. (cdc.gov)
Interleukin9
  • Growing evidence suggests that proinflammatory cytokines (PICs), such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNFα), are induced in the spinal cord under various injury conditions and contribute to pain hypersensitivity. (jneurosci.org)
  • Stimulatory and inhibitory effects of interleukin (IL)-4 and IL-13 on the production of cytokines by human peripheral blood mononuclear cells: priming for IL-12 and tumor necrosis factor alpha production. (cornell.edu)
  • Interleukin (IL)-10, IL-4, IL-13, and transforming growth factor (TGF)-beta are usually considered to be the most important macrophage-deactivating factors, with inhibitory effects on cytokine production. (cornell.edu)
  • The JES5-16E3 monoclonal antibody specifically binds to the mouse cytokine, Interleukin-10 (IL-10). (bdbiosciences.com)
  • 1: In the haematopoietic microenvironment, bone marrow stromal cells play an important role in regulating haematopoiesis by expressing various cytokines, including leukaemia inhibitory factor (LIF) and interleukin-6 (IL-6). (unboundmedicine.com)
  • TY - JOUR T1 - Role of mitogen-activated protein kinase family in serum-induced leukaemia inhibitory factor and interleukin-6 secretion by bone marrow stromal cells. (unboundmedicine.com)
  • An excessive extracellular presence of the proinflammatory cytokines interleukin 1 (IL1), tumour necrosis factor α (TNFα), and interleukin 6 (IL6), in particular, has been identified and intra-articular as well as systemic levels of these cytokines may to some extent reflect disease activity. (bmj.com)
  • Treatment of HCEpiC with 1 ng/ml IL-1β for 18 h significantly increased release of the cytokines/chemokines granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein-1 (MCP-1), while hyperosmolarity-induced release of IL-6 and MCP-1. (molvis.org)
  • Viallard et al demonstrated that, when stimulated, peripheral blood mononuclear cells of eosinophilic fasciitis patients produce significantly higher amounts of five cytokines, including interleukin (IL)-5 and interferon (IFN)-gamma. (medscape.com)
Inflammatory cytokines4
  • Over lifetime, due to the action of several stressors such as DNA damage and telomere shortening, senescent cells accumulate in the organism and release a variety of pro-inflammatory cytokines responsible for low-grade inflammation. (frontiersin.org)
  • A significant number of illnesses of individuals with alcoholic liver diseases can be explained readily by a high level of circulating pro-inflammatory cytokines[ 1 ]. (wjgnet.com)
  • MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. (rndsystems.com)
  • To inves- inflammatory cytokines and the timing of their production support tigate the interplay of proinflammatory and anti-inflammatory cyto- an immunologic response appropriate to the amount of Ag and kines in human granulomatous disease, we evaluated the ability of duration of exposure. (cdc.gov)
Macrophage migration inhibitory factors1
  • In 1974, Cohen and colleagues reported production of macrophage migration inhibitory factors in virus-infected fibroblasts, which led (finally) to proposal of the term cytokine. (cdc.gov)
Human peripheral blood mononuclear1
  • We investigated the mechanisms responsible for the regulatory effects of IVIG on in vitro human peripheral blood mononuclear cell (PBMC) cytokine production. (tmu.edu.tw)
Factor12
  • Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein that in humans is encoded by the MIF gene. (wikipedia.org)
  • Macrophage migration inhibitory factor assembles into a trimer composed of three identical subunits. (wikipedia.org)
  • Macrophage migratory inhibitory factor was identified in 1966. (cdc.gov)
  • Addition of IL-4 and IL-13 to peripheral blood mononuclear cell (PBMC) cultures inhibited production of IL-12, tumor necrosis factor (TNF)-alpha, IL-10, and IL-1 beta induced by lipopolysaccharide (LPS) or Staphylococcus aureus added simultaneously with the cytokines. (cornell.edu)
  • An alcohol-induced increase of tumor necrosis factor (TNF) α, a pro-inflammatory cytokine, in the mouse brain is closely linked to neurodegeneration[ 4 ]. (wjgnet.com)
  • MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. (rndsystems.com)
  • Peripheral lung inflammation may cause a "spill-over" of cytokines, such as interlukin (IL)-6, IL-1β and tumour necrosis factor (TNF)-α, into the systemic circulation, which may increase acute-phase proteins such as C-reactive protein (CRP). (ersjournals.com)
  • Astrocytic activation involved hypertrophy and increased expression of glial fibrillary acidic protein (GFAP), S100A10, and leukemia inhibitory factor (LIF), a cytokine that activates the STAT3 signaling pathway. (jneurosci.org)
  • The findings of Dziadzio et al support increased levels of IL-5 in eosinophilic fasciitis, in addition to increased levels of transforming growth factor (TGF)-beta, another fibrogenic cytokine. (medscape.com)
  • Analysis of leukemia inhibitory factor, type 1 and type 2 cytokine production in patients with eosinophilic fasciitis. (medscape.com)
  • Oncostatin M is a 28 kDa monomeric glycoprotein that is similar in structure to LEUKEMIA INHIBITORY FACTOR. (bvsalud.org)
  • At infection sites, activated T cells secrete cytokines (eg, interferon-gamma [IFN-gamma]) that induce production of macrophage migration inhibitory factor, preventing macrophages from leaving. (msdmanuals.com)
Anti-inflammatory3
  • Humans with variants of pro- and anti-inflammatory cytokine genes show increased susceptibility to alcoholic liver disease[ 2 ]. (wjgnet.com)
  • A cytokine with both pro- and anti-inflammatory actions that depend upon the cellular microenvironment. (bvsalud.org)
  • M2 "alternatively activated" anti-inflammatory macrophages are stimulated predominantly by cytokines such as IL-4 and IL-13. (msdmanuals.com)
Stimulatory3
  • The activation of a resting helper T cell causes it to release cytokines and other stimulatory signals (green arrows) that stimulate the activity of macrophages , killer T cells and B cells , the latter producing antibodies . (wikidoc.org)
  • as applicable for subjects in cohort 1 and cohort 2) at the time the subject is planned for leukapheresis, except for systemic inhibitory/stimulatory immune checkpoint therapy. (who.int)
  • At least 3 half-lives must have elapsed from any prior systemic inhibitory/stimulatory immune checkpoint molecule therapy at the time the subject is planned for leukapheresis (e.g. ipilimumab, nivolumab, pembrolizumab, atezolizumab, OX40 agonists, 4- 1BB agonists etc). 107. (who.int)
Secretion4
  • Pre-incubation of PBMCs with IVIG inhibited lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA)/ionomycin stimulated cytokine secretion. (tmu.edu.tw)
  • Our data also showed that cross-linking cell membrane-bound IVIG with anti-human kappa- and lambda-chain antibodies resulted in cytokine secretion levels similar to those elicited by LPS. (tmu.edu.tw)
  • However, the intracellular signal that regulates cytokine secretion in bone marrow stromal cells has not been determined. (unboundmedicine.com)
  • Furthermore, all assays designed to quantify extracellular levels of cytokines will measure the net outcome of secretion, absorption to numerous cellular and soluble cytokine receptors, and degradation by proteases present at inflammatory sites. (bmj.com)
Lymphocytes2
Cells19
  • Furthermore, glucocorticoids also stimulate white blood cells to release MIF and hence MIF partially counteracts the inhibitory effects that glucocorticoids have on the immune system. (wikipedia.org)
  • The ELISPOT assay is designed to enumerate cytokine producing cells in a single cell suspension. (abcam.com)
  • This technique is designed to determine the frequency of cytokine producing cells under a given stimulation, and the follow-up of such frequency during a treatment and/or a pathological state. (abcam.com)
  • Colored "purple" spots indicate cytokine production by individual cells. (abcam.com)
  • This phenomenon could be offset by a dual-CAR system incorporating both an activating CAR against the cognate tumor antigen and an NK self-recognizing inhibitory CAR that transferred a 'don't kill me' signal to NK cells upon engagement with their TROG + siblings. (nature.com)
  • Andersson U, Andersson J. Immunolabeling of cytokine-producing cells in tissues and in suspension. (bdbiosciences.com)
  • Cytokine Producing Cells. (bdbiosciences.com)
  • Viral regulation of B7 family inhibitory molecules in epithelial cells leads to suppression or termination of immune responses [ 19 ]. (hindawi.com)
  • This clustered distribution was not found for cells expressing any of the other studied cytokines. (bmj.com)
  • 6-16 However, most previous studies based on immunohistochemical staining of intra-articular cytokine production in RA have been performed on acetone fixed tissue, a procedure which does not readily allow subsequent discrimination of cytokine producing cells and cytokine binding cells. (bmj.com)
  • Apoptosis analysis displayed no effect in A549 and inhibitory effect of CNC in BEAS-2B cells. (cdc.gov)
  • Furthermore, the signaling mechanisms of the cells were precisely defined by their cytokine responses. (cdc.gov)
  • The MeOH extract of Ganoderma lucidum has an inhibitory action on histamine release from rat mast cells. (researchgate.net)
  • Soluble adult worm antigen preparation (SWAP) triggers release of cytokine IL-10 from peripheral blood mononuclear cells from both healthy and infected individuals [ 505 ], and the IL-10 then suppresses lymphoproliferative responses to SWAP by 90-100% [ 504 ]. (nanomedicine.com)
  • A dysregulated immune system (including changes in the number and/or function of immune cells, cytokine levels, and the expression of inhibitory receptors or their ligands) plays a key role in the development of HCC. (wjgnet.com)
  • In this review, we have discussed the status and roles of various immune effector cells ( e.g ., dendritic cells, natural killer cells, macrophages, and T cells), their cytokine profile, and the chemokine-receptor axis in promoting or impeding HCC. (wjgnet.com)
  • When cells were treated with IL-1β and EGCG or hyperosmolarity and EGCG there was a dose-dependent reduction in release of these cytokines/chemokines, with significant inhibition observed at 3-30 µM. (molvis.org)
  • The autoimmune nature of diabetes and inflammatory cytokine IL-10 (as markers the major contribution of lymphocyte T- of inflammatory changes) and the soluble cells are well established. (who.int)
  • Our data demonstrate that beryllium-stimulated bronchoalveolar lavage cells produce IL-10, and the neutralization of endogenous IL-10 does not increase significantly cytokine production, HLA expression, or T lymphocyte pro- liferation. (cdc.gov)
Monocytes1
  • The production of cytokines in monocytes/macrophages is regulated by several different cytokines that have activating or inhibitory effects. (cornell.edu)
Activates2
  • In this study, we proposed to investigate how these interactions work between RANKL/RANK and other cytokines' signaling downstream that activates TRAF6 transcriptome and thus induces the osteoclastogenesis in vitro . (umich.edu)
  • An increased expression of lesional CCL20 can recruit CCR6+ Th17, and lesional cytokine milieu persistently activates keratinocytes to produce CCL20. (nih.gov)
Interferon-gamma1
Production8
  • This method has the advantage of requiring a minimum of in-vitro manipulations allowing cytokine production analysis as close as possible to in-vivo conditions in a highly specific way. (abcam.com)
  • 4: Expression of a dominant-negative mutant form of JNK1 that blocked FCS-induced JNK activity had no effect on protein production and gene expression of these cytokines. (unboundmedicine.com)
  • 1-5 The paracrine or autocrine mode of action of most cytokines complicates the interpretation of studies of body fluids for reliable assessment of cytokine production. (bmj.com)
  • Techniques based on immunohistochemistry have thus also been widely used to study the expression of cytokines within the synovial tissue in attempts to determine true local production. (bmj.com)
  • Cellular proliferation and cytokine production was measured. (bvsalud.org)
  • We hypothesized that, in CBD, failure of IL-10 to modulate the beryllium-specific, cell-mediated immune response would result in persistent, maximal cytokine production and T lymphocyte proliferation, thus contributing to the development of granulomatous lung disease. (cdc.gov)
  • exogenous rhIL-10 has a limited effect on cytokine production and no effect on T lymphocyte proliferation or HLA expression. (cdc.gov)
  • ance in the timing or production of these cytokines, resulting in IL-10 is a key inhibitory cytokine in the inflammatory response tissue damage and disease. (cdc.gov)
Inflammation3
  • This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. (thermofisher.com)
  • RESULTS A wide variation between subjects was recorded for both quantities and profiles of expressed cytokines, despite similar macroscopic and histopathological features of inflammation. (bmj.com)
  • Cytokines, locally produced in the synovial membrane of patients with rheumatoid arthritis (RA), play an important part in regulating the chronic joint inflammation which may lead to serious tissue damage, such as cartilage degradation, bone resorption, and fibrosis. (bmj.com)
Receptors1
  • Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. (abcam.com)
GDF151
  • Macrophage Inhibitory Cytokine 1 (MIC-1/GDF15) is a biomarker that may be expressed by some cancers. (examine.com)
Monoclonal antibody1
  • After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. (abcam.com)
Antibodies2
Proliferation1
  • The increased proliferation was synergistic and mediated by both pro-inflammatory and pro-carcinogenic cytokines. (cdc.gov)
Molecules2
  • After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. (abcam.com)
  • Some studies suggest that coregulatory molecules and cytokines may play a role [ 18 ]. (hindawi.com)
Soluble1
  • ABSTRACT This study assessed changes in serum levels of cytokines IFN and IL-10 (biomarkers of in ammatory changes) and soluble biomarkers sICAM-1 and sE-selectin (biomarkers of endothe- lial dysfunction) in diabetic patients with and without nephropathy. (who.int)
Protein2
  • Although the term cytokine had not yet even been defined, interferon-α, the first cytokine known, was identified in 1957 as a protein that interfered with virus replication. (cdc.gov)
  • Conclusion: Human AAT is an acute phase protein with a broad-protease inhibitory and immunomodulatory activities used as a therapeutic for emphysema patients with inherited AAT deficiency. (lu.se)
Pathways1
  • Further evaluation of 1 mg CEA peptide with stronger adjuvants, and/or combined with agents to overcome immune inhibitory pathways, may be warranted in PC pts. (bmj.com)
Milieu1
  • The liver plays a central role in detoxifying LPS and producing a balanced cytokine milieu. (wjgnet.com)
Mechanisms2
  • Together, our data have demonstrated that PICs induce central sensitization and hyperalgesia via distinct and overlapping synaptic mechanisms in superficial dorsal horn neurons either by increasing excitatory synaptic transmission or by decreasing inhibitory synaptic transmission. (jneurosci.org)
  • The main research aim is to examine a variety of antagonists to identify those with possible therapeutic inhibitory effects on mesothelioma growth and determine the mechanisms underlying the contribution to tumour growth. (edu.au)
Eosinophilic fasciitis1
  • Dziadzio L, Kelly EA, Panzer SE, Jarjour N, Huttenlocher A. Cytokine abnormalities in a patient with eosinophilic fasciitis. (medscape.com)
Proteins1
  • From the Greek cyto (cavity or cell) and kine (movement), cytokines are proteins involved in cell signaling and function as immunomodulating agents. (cdc.gov)
Cell1
  • Taken together, the findings of these studies suggest a mechanistic framework marked by a proinflammatory and fibrogenic cytokine response with resultant tissue inflammatory cell infiltration. (medscape.com)
Microbial1
  • M1, "classically activated," pro-inflammatory macrophages are stimulated by cytokines such as IFN-gamma and by various microbial components (eg, lipopolysaccharide). (msdmanuals.com)
Effects3
  • Using patch-clamp recordings in lamina II neurons of isolated spinal cord slices, we compared the effects of IL-1β, IL-6, and TNFα on excitatory and inhibitory synaptic transmission. (jneurosci.org)
  • Black arrows mark the positive effects between elements, whereas red stop bars denote inhibitory effects. (hindawi.com)
  • Luminex technology was used to determine the effects of EGCG (0.3 - 30 µM) on IL-1β- or hyperosmolar-induced cytokine release into the medium. (molvis.org)
Phenotype1
  • IL-35 not only enhanced inhibitory function of Tregs but also suppressed phenotype shift of Tregs into Th22 phenotype in PBC patients. (bvsalud.org)
Staphylococcus1
CONCLUSION1
  • Conclusion: These findings suggest that RANKL can work alone or interactively with critical cytokines (i.e. (umich.edu)
Bone1
  • We will apply the novel information to address the important cytokine interactions critical to normal vs. pathologic bone conditions in health and disease. (umich.edu)
Interaction1
  • In this study, we explored an inhibitory effect of statins on CCL20/CCR6 interaction. (nih.gov)
Role1
  • Cytokines play an important role in promoting wound healing and tissue repair. (wikipedia.org)