• AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. (neobiotechnologies.com)
  • This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. (neobiotechnologies.com)
  • The distribution patterns of these complexes differ considerably in different epithelial cells, so that the origin of these cytokeratins can be narrowed down with an antibody detection against the subtypes KRT1 to KRT20. (zxc.wiki)
  • IHC analysis of human kidney using anti-Keratin K19 antibody (Cat. (progen.com)
  • The primary antibody anti-Keratin K19 (Cat. (progen.com)
  • The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. (progen.com)
  • IHC analysis of human head and neck squamous cell carcinoma using anti-Keratin K19 antibody (Cat. (progen.com)
  • Western blot analysis of MCF-7 lysate with anti-Keratin K19 antibody (Cat. (progen.com)
  • The following product was used in this experiment: Cytokeratin 12 Recombinant Rabbit Monoclonal Antibody (2M1K10) from Thermo Fisher Scientific, catalog # MA5-42701, RRID AB_2911842. (thermofisher.com)
  • Samples were incubated with Cytokeratin 12 Monoclonal antibody (Product # MA5-42701) using a dilution of 1:100 (40x lens). (thermofisher.com)
  • The antibody specifically reacts with keratins K5 and K8 present in nearly all epithelia. (progen.com)
  • MCF-7 cells were subjected to SDS PAGE followed by western blot with 60187-1-Ig (Cytokeratin 19 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 10x lens. (ptglab.com)
  • Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60187-1-Ig (Cytokeratin 19 antibody) at dilution of 1:51200 (under 40x lens. (ptglab.com)
  • The antibody detects endogenous Cytokeratin 18 protein. (abbkine.com)
  • 2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year. (abbkine.com)
  • Monoclonal Antibody Analysis of Keratin Expression in Epidermal Diseases: A 48- and 56-kdalton Keratin as Molecular Markers for Hyperproliferative Keratinocytes. (novodiax.com)
  • Immunolocalization of Keratin Polypeptides in Human Epidermis Using Monoclonal Antibodies. (novodiax.com)
  • All tumors expressed cytokeratin 7 and gross cystic disease fluid protein 15 and were negative for cytokeratin 20 expression. (duke.edu)
  • There are currently 20 known cytokeratin proteins (see table) with a molecular mass between 40 and 68 kDa. (zxc.wiki)
  • Keratin ( / ˈ k ɛr ə t ɪ n / [1] [2] ) is one of a family of structural fibrous proteins also known as scleroproteins . (wikipedia.org)
  • Keratins act as cytoprotective proteins. (sigmaaldrich.com)
  • Cytokeratin 17 is a member of the cytokeratin subfamily of intermediate filament proteins which are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. (bio-biz-navi.com)
  • Background: This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. (icbinhibitor.com)
  • Keratins are heteropolymeric structural proteins which form the intermediate filament. (icbinhibitor.com)
  • The Scalar Light Amino Acid and Protein Program operates twenty-four (24) hours per day seven (7) days per week thereby assuring a constant creation and delivery of amino acids and proteins to the quantum, human body. (scalarlight.com)
  • Keratin 20, often abbreviated CK20, is a protein that in humans is encoded by the KRT20 gene. (wikipedia.org)
  • Spider silk is classified as keratin, [9] although production of the protein may have evolved independently of the process in vertebrates. (wikipedia.org)
  • The new term corneous beta protein (CBP) has been proposed to avoid confusion with α-keratins. (wikipedia.org)
  • Recombinant fusion protein containing a sequence corresponding to amino acids 250-494 of human Cytokeratin 12 (KRT12) (Q99456). (thermofisher.com)
  • Storage Buffer: 20 mM Tris-Borate, 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% sodium azide, pH 7.5. (icbinhibitor.com)
  • Keratin is a structural protein from the intermediate filament network, i.e., responsible for maintaining the keratinocyte's structural integrity. (ijpsr.com)
  • Keratin 16 is a type I intermediate filament protein present on the tongue, hair follicle and a part of glabrous skin 1 . (ijpsr.com)
  • Keratinocytes were packed with protein and converted into the hard cell, generating unique keratin structurally and functionally 2, 3 . (ijpsr.com)
  • Cytokeratin and keratin 16 make a protein, i.e., filaggrin (protein maker). (ijpsr.com)
  • About 54 members of related protein were present in a keratin family. (ijpsr.com)
  • Keratin is a protein found in nails, hair and skin that forms tough fibers for strength. (ijpsr.com)
  • Here we investigated the role of IPO13 in the pathogenesis of pterygium and the underlying mechanism including interaction with other cell proliferation-related factors: keratin 17 (K17), a lesional protein and a member of the type I keratins, and c-Jun, a protein of the activator protein-1 complex. (molvis.org)
  • Using RT-PCR, Western blotting and immunostaining, we show that synemin M is present at both mRNA and protein levels in undifferentiated ES cells as early as pluripotency factor Oct-3/4 and IF keratin 8. (biomedcentral.com)
  • Electron microscopy reveals loss of desmosomes (epithelial intercellular junctions formed by membrane and submembrane protein complexes), breakdown of desmosome-keratin intermediate filament attachment, and perinuclear aggregates of keratin intermediate filaments. (medscape.com)
  • [ 25 ] In particular, studies have revealed that keratosis follicularis (Darier disease) keratinocytes displayed abnormal trafficking of the desmosomal protein desmoplakin and abnormal expression of cytokeratins 10 and 14. (medscape.com)
  • Immunohistochemical study of cytokeratin expression in nevus sebaceous. (harvard.edu)
  • Morikawa T, Takizawa H, Ohnishi T, Watanabe S. Dilated pore: a case report and an immunohistochemical study of cytokeratin expression. (medscape.com)
  • Using the source a bit more to explain what each gene and each zone of genes mean will be helpful, as we currently have no particular examples of a hair keratin. (wikipedia.org)
  • Mutations in the gene for keratin-6 have been associated with PACHYONYCHIA CONGENITA, TYPE 1. (harvard.edu)
  • This gene is located within a cluster of keratin family members on chromosome 17q21. (icbinhibitor.com)
  • In this review, we have discussed the keratinization that occurs in the stratum corneum that follows mutation in the keratin gene "rare disease" pachyonychia congenital an autosomal dominant disorder or hereditary syndrome by any one of a missense mutation in keratin genes such as KRT6A, KRT6B, KRT16 and KRT17. (ijpsr.com)
  • Type I pachyonychia congenita or type I keratin (k9-k20) keratin gene with two chromosomal loci that were clustered on 17q12 and q21 chromosomes. (ijpsr.com)
  • Type II pachyonychia congenital or type II keratin (k1-k8) keratin gene with chromosomal loci that were clustered and has been located on 12q11q-14. (ijpsr.com)
  • Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. (abnova.com)
  • Mutations in the gene for keratin-12 have been associated with MEESMANN CORNEAL EPITHELIAL DYSTROPHY. (wakehealth.edu)
  • The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. (bio-biz-navi.com)
  • Immunohistochemical studies showed that the neoplastic cells were SMA+, CD10+, and PR+, while they were negative for desmin and keratin. (hindawi.com)
  • alpha-Keratins have been classified into at least 20 subtypes. (curehunter.com)
  • A type I keratin that is found associated with the KERATIN-3 in the CORNEA and is regarded as a marker for corneal-type epithelial differentiation. (wakehealth.edu)
  • ALES is a rare subtype that demonstrates the EWSR1-FLI1 translocation characteristic of ES and demonstrates complex epithelial differentiation including diffuse cytokeratin and p40 expression. (bvsalud.org)
  • Immunohistochemistry analysis of Cytokeratin 12 in paraffin-embedded mouse cornea. (thermofisher.com)
  • The relationships of LKB1 expression to clinic-pathological parameters and epithelial markers E-cadherin and high-molecular-weight -cytokeratin (HMW-CK) were investigated in 80 clinical breast cancer tissue samples and their paired normal control breast tissue samples by using immunohistochemistry. (biomedcentral.com)
  • A type I keratin expressed in a variety of EPITHELIUM, including the ESOPHAGUS, the TONGUE, the HAIR FOLLICLE and NAILS. (harvard.edu)
  • It was shown in the waist, hips, knees, and elbows, with excessive development of keratin in the hair follicle and has shown in that areas where lots of friction occurred 7, 8 . (ijpsr.com)
  • Cytokeratin 19 expression was restricted to the basal cell layers, and cytokeratin 4 expression was abundant in suprabasal cells. (tamu.edu)
  • The human genome encodes 54 functional keratin genes , located in two clusters on chromosomes 12 and 17. (wikipedia.org)
  • This pathway describes progression of a serrated precursor lesion, often followed by the onset of epigenetic instability involving promoter methylation and silencing of key tumour suppressor genes, and accounts for 15%-20% of sporadic colorectal cancer [ 1 , 2 ]. (hindawi.com)
  • KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. (thermofisher.com)
  • KRT18 encodes the type I intermediate filament chain keratin 18. (abnova.com)
  • formerly also designated cytokeratin 20). (progen.com)
  • It was capable to effectively decompose feathers or "soft" keratin of stratum corneum , in contrast to other "hard" hair-type keratins. (scielo.br)
  • Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms a coiled-coil alpha helical structure consisting of TYPE I KERATIN and a TYPE II KERATIN, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. (curehunter.com)
  • The antiserum was produced against synthesized peptide derived from human Keratin 8. (sigmaaldrich.com)
  • Keratin and filaggrin expression in keratoacanthoma. (harvard.edu)
  • Recognition of OOC as a unique entity has long been due, yet its inexplicable radiographic presentation resembling dentigerous cyst, histological likeness to odontogenic keratocyst (OKC) and inconsistent cytokeratin expression profiles overlapping with both as well as with the epidermis, makes it rather confounding. (bvsalud.org)
  • Knocking down synemin in ES cells by shRNA lentiviral particles transduction has no influence on expression of Oct4, Nanog and SOX2, but decreased keratin 8 expression. (biomedcentral.com)
  • Expression of keratin 5 as a distinctive feature of epithelial and biphasic mesotheliomas. (novodiax.com)
  • Histological analyses indicated the formation of well-differentiated squamous carcinoma cells exhibiting pronounced stratification (basal and suprabasal cells) and keratinization (keratin pearls) with abundant stroma. (tamu.edu)
  • The presented M. luteus isolate exhibits a significant keratinolytic potential, which determines its feasible applicatory capacity towards biodegradation of poultry by-products or formulation of keratin-based feed components. (scielo.br)
  • EMC is a low-grade malignancy with a recurrence rate of 30%-50%, with chances of lymph node metastases being 15%-20% and 5-year and 10-year survival rates of 80%-94% and 72%-90%, respectively. (ijhnp.org)
  • Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane BE: Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. (progen.com)
  • The human keratins: biology and pathology. (novodiax.com)
  • The diagnosis of pulmonary metastases was obtained by reviewing the histology of the previous uterine tumor: the tumor cells were immunoreactive for CD10, PR, and smooth muscle actin (SMA), but negative for desmin, S100, CD34, CD 117, cytokeratins AE1AE3, CD68R, and ER. (hindawi.com)
  • Epitope has been mapped to aa 353-367 on alpha helical rod domain of Keratin K8 (Waseem et al. (progen.com)
  • Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. (progen.com)
  • Histopathological examination of the hematoxylin-eosin (HE)-stained sections showed a cystic cavity lined by orthokeratinized stratified squamous epithelium lining, with surface showing sheaves of keratin arranged in many layers. (bvsalud.org)
  • From keratin-containing natural products are L cysteine, L - tyrosine and other proteinogenic amino acids on an industrial scale produced. (zxc.wiki)
  • Before cornification and generally in epithelia of vertebrates and other animal groups, α-keratins (or cytokeratins) are in the form of loosely organized keratin filaments. (zxc.wiki)
  • In pairs, these form a heterodimer complex in the intermediate filaments of a type A and a type B cytokeratin. (zxc.wiki)
  • Keratin monomers assemble into bundles to form intermediate filaments , which are tough and form strong unmineralized epidermal appendages found in reptiles , birds , amphibians , and mammals . (wikipedia.org)
  • Keratins (also described as cytokeratins ) are polymers of type I and type II intermediate filaments that have been found only in chordates ( vertebrates , amphioxus , urochordates ). (wikipedia.org)
  • [11] Recent scholarship has shown that sauropsid β-keratins are fundamentally different from α-keratins at a genetic and structural level. (wikipedia.org)
  • Alpha-keratin (α-keratin) is a type of keratin found in vertebrates . (wikipedia.org)
  • Alpha-keratins (α-keratins) are found in all vertebrates. (wikipedia.org)
  • The keratin in horns and claws contains more disulfide bridges than that in wool and hair. (zxc.wiki)