• NADPH-cytochrome P450 reductase (CPR) and cytochrome- b 5 ( b 5 ) together with NADH- b 5 reductase ( b 5 R) play important roles in cytochrome P450 3A-mediated drug metabolism via electron transfer. (aspetjournals.org)
  • RNA interference of NADPH-cytochrome P450 reductase increases the susceptibility of Aphis gossypii Glover to sulfoxaflor. (bvsalud.org)
  • NADPH-cytochrome P450 reductase ( CPR ) is essential for the detoxification of endogenous and exogenous substances mediated by cytochrome P450 . (bvsalud.org)
  • Candida maltosa NADPH-cytochrome P450 reductase: cloning of a full-length cDNA, heterologous expression in Saccharomyces cerevisiae and function of the N-terminal region for membrane anchoring and proliferation of the endoplasmic reticulum. (mdc-berlin.de)
  • Disruption of the 'Saccharomyces cerevisiae' Gene for NADPH-Cytochrome P450 Reductase Causes Increased Sensitivity to Ketoconazole. (epa.gov)
  • Differentiation of prohaptens from direct acting contact chemical allergens using a cytochrome p450 reductase deficient mouse model. (cdc.gov)
  • In vivo, nitrite conversion to nitric oxide requires the nitrite reductase activity of xanthine oxidase and cytochrome P-450, thus the involvement of these enzymes was also evaluated. (unical.it)
  • Specific inhibition of xanthine oxidase and cytochrome P-450 revealed, in the two teleosts, that the nitrite-dependent inotropism required the nitrite reductase activity of both enzymes. (unical.it)
  • Results suggested that in the heart of C. hamatus and T. bernacchii, nitrite is an integral physiological source of nitric oxide with important signaling properties, which require the nitrite reductase activity of xanthine oxidase and cytochrome P-450. (unical.it)
  • The redox partner of microsomal CYPs (including CYP1A1) is NADPH cytochrome P450 oxidoreductase (CPR). (biologists.com)
  • Cytochrome c-556 was identified in a bacterial periplasmic fraction as a redox component which couldbe oxidised by TMAO or DMSO. (uea.ac.uk)
  • The mid-point redox potential (pH 7.6) of cytochrome c-556 was + 105 mV (n = 1). (uea.ac.uk)
  • We also found that xanthine oxidase is more expressed in C. hamatus than in T. bernacchii, while the opposite was observed concerning cytochrome P-450. (unical.it)
  • The high potential chain, consisting of the iron sulfur protein, cyt c1 and cyt c2, transfers the first electron from quinol to an acceptor (cytochrome oxidase). (yeastgenome.org)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) in Tissue homogenates and other biological fluids. (orbitalbiosciences.com)
  • Description: A sandwich ELISA kit for detection of Ubiquinol Cytochrome C Reductase Core Protein II from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (orbitalbiosciences.com)
  • Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation. (childrensmercy.org)
  • UniProt: Cytochrome c-type protein NrfB. (unam.mx)
  • Nitric oxide reductase (cytochrome c) (EC 1.7.2.5) is an enzyme with systematic name nitrous oxide:ferricytochrome-c oxidoreductase. (wikipedia.org)
  • H. capsulatum contains a P450 nitric oxide reductase homologue (NOR1) that may be important for detoxifying NO during infection. (nih.gov)
  • Spectral characterization of Nor1p indicated that it was similar to other fungal P450 nitric oxide reductases. (nih.gov)
  • Together, these results provide evidence for a protective role of a P450 nitric oxide reductase against macrophage-derived NO. (nih.gov)
  • These prices, cloned and expressed nitric oxide synthase structurally resembles cytochrome p reductase. (njacs.org)
  • Warfarin is metabolized by hepatic cytochrome P-450 (CYP) isoenzymes predominantly to inactive hydroxylated metabolites, which are excreted in the bile. (medscape.com)
  • The role of cytochromes in the electron-transport pathway to trimethylamine N-oxide (TMAO)/dimethylsulphoxide (DMSO) reductase in the photosynthetic bacterium Rhodobacter capsulatus was investigated. (uea.ac.uk)
  • Reduced-minus-oxidized difference spectra in intact cells with TMAO or DMSO as oxidant revealed cytochrome absorbance changes with a maximum at 559 nm and a shoulder between 548 nm and 556 nm. (uea.ac.uk)
  • Cytochrome c-556 was the only cytochrome species which co-fractionated with TMAO/DMSO reductase following gel filtration of a post-chromatophore supernatant produced after French presstreatment of intact cells. (uea.ac.uk)
  • It is suggested that TMAO/DMSO reductase and cytochrome c-556 form a structural and functional association in the periplasm of Rhodobacter capsulatus. (uea.ac.uk)
  • The former change indicates a role for a 6-type cytochrome and the latter for a c-type cytochrome, both of which are distinct from the cytochrome bc1 complex. (uea.ac.uk)
  • Definition of the homologous of bovine subunit VII of ubiquinol-cytochrome C reductaseshould facilitate further analysis of structure/function relationships of silkworm cytochrome bcl complex. (researchgate.net)
  • In this study, we characterized the small subunit of ubiquinol-cytochrome C reductase (Bmuccr) of the silkwormBombyx mori, a model insect of Lepidopteron species. (researchgate.net)
  • The ORF contained 354bp and encoded 117 amino acid residues, which shares 69% overall amino acid sequenceidentities with the subunit VII of ubiquinol-cytochrome C reductase from bovine. (researchgate.net)
  • Phenazopyridine induced methaemoglobinaemia associated with decreased activity of erythrocyte cytochrome b5 reductase. (bmj.com)
  • The downregulated DEGs were mainly enriched in digestion, basal part of the cell, and aldo-keto reductase (NADP) activity. (hindawi.com)
  • And the above pathways were enriched primarily in the metabolism of xenobiotics by cytochrome P450, drug metabolism-cytochrome P450, and retinol metabolism. (hindawi.com)
  • We have investigated the degradation in rat liver of two typical endoplasmic reticulum (ER) membrane proteins, phenobarbital (PB)-inducible cytochrome P-450 (P-450[PB]) and NADPH-cytochrome P-450 reductase (FP2). (rupress.org)
  • This is achieved by oxidizing ubiquinol (ubihydroquinone) which reacts from the membrane phase, reducing cytochrome c in the intermembrane space, and using the free energy change to transport H+ ions across the membrane from the matrix to the inter membrane space. (yeastgenome.org)
  • Coumarins and related compounds inhibit vitamin K1 -2,3 epoxide reductase, preventing vitamin K from being reduced to its active form. (medscape.com)
  • Cytochrome P450s (CYP450s) are part of the superfamily of xenobiotic-metabolising enzymes that have been investigated for their effects on various drugs and toxins. (biologists.com)
  • The patient and several members of her family showed decreased activities of erythrocyte NADH-cytochrome b5 reductase, predisposing them to the development of clinically significant methaemoglobinaemia when challenged with oxidant drugs. (bmj.com)
  • Current therapies to reduce obstruction, such as α1-adrenoceptor antagonists and 5α-reductase inhibitors, are not effective in all patients. (lu.se)
  • Overexpression of NADPH-cytochrome P450 reductase is associated with sulfoxaflor resistance and neonicotinoid cross-resistance in Nilaparvata lugens (Stål). (bvsalud.org)
  • Nicotinamide adenine dinucleotide phosphate ( NADPH )- cytochrome P450 reductase ( CPR ), a crucial electron -transfer partner of P450 systems, is required for various biological reactions catalyzed by P450 monooxygenase . (bvsalud.org)
  • Cytochromes P450 reconstituted with NADPH: P450 reductase mimic the activating and detoxicating metabolism of the anticancer drug ellipticine in microsomes. (nel.edu)
  • Purified CYP enzymes reconstituted with NADPH:CYP reductase oxidized ellipticine to up to five metabolites, 7-hydroxy-, 9-hydroxy-, 12-hydroxy-, 13-hydroxyellipticine and ellipticine N(2)-oxide. (nel.edu)
  • The results showed that the system of purified CYPs reconstituted with NADPH: CYP reductase proved for ellipticine oxidation provide a true reflection of the situation in the microsomal membrane. (nel.edu)
  • Kotrbová V, Aimová D, Brezinová A, Janouchová K, Poljaková J, Frei E, Stiborová M. Cytochromes P450 reconstituted with NADPH: P450 reductase mimic the activating and detoxicating metabolism of the anticancer drug ellipticine in microsomes. (nel.edu)
  • 10. Interflavin one-electron transfer in the inducible nitric oxide synthase reductase domain and NADPH-cytochrome P450 reductase. (nih.gov)
  • 15. Role of Ser457 of NADPH-cytochrome P450 oxidoreductase in catalysis and control of FAD oxidation-reduction potential. (nih.gov)
  • Microsomal electron transport chain: monooxygenase activities and relationships between NADPH-cytochrome c-reductase and cytochrome P450 in mouse liver. (unibo.it)
  • The oxidation of isocitrate through IDH activity was coupled to the cytochrome system by NADPH- and NADH-cytochrome c reductase activities. (unboundmedicine.com)
  • Diphenyleneiodonium was the most potent and inhibited NADPH-cytochrome P450 reductase as well, whereas apocynin and Tiron did not. (uncg.edu)
  • NADPH cytochrome P-450 reductase releases FMN and FAD upon dilution into slightly acidic potassium bromide. (tmc.edu)
  • Microsomal protein level, NADPH cytochrome c reductase activity, and the activities and protein levels of cytochrome P-450 isozymes CYP1A1 and CYP2B1 were monitored to assess the effects of AFC exposure on pulmonary P-450. (cdc.gov)
  • Emodin, doxorubicin and organic extracts of C. obtusifolia inhibited NADH:cytochrome c oxidoreductase activity of bovine heart mitochondrial particles and NADH:CoQ oxidoreductase activity of porcine heart mitochondrial NADH dehydrogenase, whereas juglone was stimulatory. (nih.gov)
  • The expression of glutathione (GSH)-dependent enzymes and cytochrome P450 (P450) proteins in freshly isolated proximal tubular cells from human kidney (hPT), and the effect of primary culture on these enzymes, were determined. (aspetjournals.org)
  • Like UQCRC1 in preventing cytochrome c from release, functions of ETC proteins beyond oxidative phosphorylation might also contribute to the pathogenesis of PD. (frontiersin.org)
  • The second problem is linked to the evolution of important enzymatic systems: cytochromes and ABC proteins. (hindawi.com)
  • Cytochromes and ABC proteins, which evolved over time to detoxify food from vegetable chemical "actives," now seem to limit the action of herbal derivatives. (hindawi.com)
  • The molecular structure was compared with those of rat NADH-cytochrome b(5) reductase and corn nitrate reductase. (nih.gov)
  • A model of the complex between cytochrome b(5) and the human reductase has been built and compared with that of the haem-containing domain of the nitrate reductase molecule. (nih.gov)
  • The interaction between cytochrome b(5) and the human reductase differs from that of the nitrate reductase because of differences in the amino-acid sequences. (nih.gov)
  • Nitric oxide reductase (cytochrome c) (EC 1.7.2.5) is an enzyme with systematic name nitrous oxide:ferricytochrome-c oxidoreductase. (wikipedia.org)
  • Structural and functional characteristics of subunits of bovine heart cytochrome-c reductase have been investigated by controlled digestion of soluble and membrane-reconstituted purified bc1 complex and direct amino acid sequencing of native and digested protein subunits. (uniba.it)
  • Cytochrome b5 reductase is an enzyme in the blood. (medlineplus.gov)
  • In order to examine the function of the enzyme, the structure of NADH-cytochrome b(5) reductase from human erythrocytes has been determined and refined by X-ray crystallography. (nih.gov)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2) in Tissue homogenates and other biological fluids. (orbitalbiosciences.com)
  • These results demonstrate that AFC exposure induced CYP1A1 activity and increased the enzyme levels of CYP1A1 in lung microsomes, suggesting that AFC exposure may alter metabolism of PACs by the cytochrome P-450 system in the lung. (cdc.gov)
  • Description: A sandwich ELISA kit for detection of Ubiquinol Cytochrome C Reductase Core Protein II from Mouse in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (orbitalbiosciences.com)
  • 14478 3-oxoacyl-[acyl-carrier protein] reductase fabG BBZA01000001 CDS ARMA_0014 complement(14430. (go.jp)
  • A four minute incubation in FAD reconstituted one half to all of the specific activity, per milligram protein, of untreated reductase, depending upon the substrate. (tmc.edu)
  • Neese, F. New Insights into the Nature of Observable Reaction Intermediates in Cytochrome P450 NO Reductase by Using a Combination of Spectroscopy and Quantum Mechanics/Molecular Mechanics Calculations. (mpg.de)
  • 1. Functional interactions in cytochrome P450BM3: flavin semiquinone intermediates, role of NADP(H), and mechanism of electron transfer by the flavoprotein domain. (nih.gov)
  • 17. The flavoprotein component of the Escherichia coli sulfite reductase: expression, purification, and spectral and catalytic properties of a monomeric form containing both the flavin adenine dinucleotide and the flavin mononucleotide cofactors. (nih.gov)
  • It had little flavin dependence, was equimolar in FMN and FAD, and had nearly the specific activity (per mole flavin) of untreated reductase. (tmc.edu)
  • Freshly isolated hPT cells had relatively high activities of γ-glutamyltransferase, γ-glutamylcysteine synthetase, glutathione S -transferase (GST), glutathione disulfide reductase, and GSH peroxidase. (aspetjournals.org)
  • Three-hydroxy-3-methylglutaryl coenzyme-A reductase inhibitors (statins) are first-line treatments for hypercholesterolemia. (nih.gov)
  • Studies compared the multiple-dose pharmacokinetic interaction profiles of pravastatin, simvastatin, and atorvastatin when coadministered with 4 inhibitors of cytochrome P450-3A4 isoenzymes in healthy subjects. (nih.gov)
  • Pravastatin has a neutral drug interaction profile relative to cytochrome P450-3A4 inhibitors, but these substrates markedly increase systemic exposure to simvastatin and atorvastatin. (nih.gov)
  • NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and a cytoplasmic 2-electron reductase. (thermofisher.com)
  • The known components of the ETC are a cytochrome c, two additional cytochromes, but no quinone. (kenyon.edu)
  • 12. Electron transfer in human methionine synthase reductase studied by stopped-flow spectrophotometry. (nih.gov)
  • x]The membrane bound sulfur reducing complex is also comprised of nine major polypeptides including a hydrogenase, sulfur reductase, an electron-transport chain. (kenyon.edu)
  • Recently, we found that after cytochrome P450 (CYP)-mediated oxidation ellipticine forms covalent DNA adducts. (nel.edu)
  • Erythrocyte NADH-cytochrome b(5) reductase reduces methaemoglobin to functional haemoglobin. (nih.gov)
  • 2. Functional interactions in cytochrome P450BM3. (nih.gov)
  • Dependence upon FAD is consistent with existing hypotheses for the catalytic cycle of the reductase. (tmc.edu)
  • Bennati, M. ENDOR Spectroscopy and DFT Calculations: Evidence for the Hydrogen-Bond Network Within α2 in the PCET of E. coli Ribonucleotide Reductase. (mpg.de)
  • 9. Kinetic, spectroscopic and thermodynamic characterization of the Mycobacterium tuberculosis adrenodoxin reductase homologue FprA. (nih.gov)
  • 7. Affinity isolation and characterization of cytochrome P450 102 (BM-3) from barbiturate-induced Bacillus megaterium. (nih.gov)
  • 8. Expression, purification, and characterization of Bacillus subtilis cytochromes P450 CYP102A2 and CYP102A3: flavocytochrome homologues of P450 BM3 from Bacillus megaterium. (nih.gov)
  • 18. Design of a cytochrome P450BM3 reaction system linked by two-step cofactor regeneration catalyzed by a soluble transhydrogenase and glycerol dehydrogenase. (nih.gov)
  • Alteration of pulmonary cytochrome P-450 system: effects of asphalt fume condensate exposure. (cdc.gov)
  • The human reductase resembles the rat reductase in overall structure as well as in many side chains. (nih.gov)
  • 5. On the domain structure of cytochrome P450 102 (BM-3): isolation and properties of a 45-kDa FAD/NADP domain. (nih.gov)
  • Five mutations in the FAD-binding domain seem to be related to cytochrome b(5). (nih.gov)
  • Nevertheless, there is a large main-chain shift from the human reductase to the rat reductase or the corn reductase caused by a single-residue replacement from proline to threonine. (nih.gov)
  • The structures around 15 mutation sites of the human reductase have been examined for the influence of residue substitutions using the program ROTAMER. (nih.gov)
  • The overall molecular structures of the monoclinic and tetragonal forms of cytochrome b 1 are essentially identical. (iucr.org)
  • Neese, F. The Reaction Mechanism of Cytochrome P450 NO Reductase: A Detailed Quantum Mechanics/Molecular Mechanics Study. (mpg.de)
  • The statin (HMG-CoA reductase inhibitor) class of drugs has revolutionized the treatment of hypercholesterolemia. (medscape.com)