The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. (wikipedia.org)
Cytochrome P-450 isozymes involved in the oxidative metabolism of delt. (erowid.org)
The regio- and stereoselective metabolism of phenanthrene (PA) by seven cDNA-expressed human and five rodent and rabbit cytochromes P-450 has been examined using reverse-phase and chiral stationary phase high-pressure liquid chromatography (HPLC). (nih.gov)
Regioselectivities for PA metabolism were found to be both isozyme and species-dependent. (nih.gov)
The metabolism of riluzole is mostly hepatic and consists of cytochrome P450-dependent hydroxylation and glucuronidation. (nih.gov)
Cytochrome P450 isozymes involved in the metabolism of phenol , a benzene metabolite. (cdc.gov)
TBZ variably undergoes extensive first-pass metabolism to active metabolites, some of which are metabolized by the cytochrome P450 2D6 isozyme. (nih.gov)
CYP2D6 is a member of the cytochrome P450 family of enzymes involved in the oxidative metabolism of drugs. (medscape.com)
Results from clinical studies suggest that pregnancy alters hepatic drug metabolism in a cytochrome P450 (P450) isoform-specific manner, and rising concentrations of female hormones are potentially responsible for the changes. (aspetjournals.org)
The factors responsible for the changes in P450-mediated drug metabolism during pregnancy remain unknown. (aspetjournals.org)
Metabolism: Isozymes CYP2D6 and CYP3A3/4 are responsible for the metabolism of cevimeline. (guidelinecentral.com)
We conclude that the metabolism of midazolam and triazolam in human liver is predominantly mediated by cytochrome P450IIIA4. (aspetjournals.org)
Duloxetine undergoes predominately hepatic metabolism via two cytochrome P450 isozymes, CYP2D6 and CYP1A2. (janaushadhi.org)
The CYP P450 genes are involved in the formation and breakdown (metabolism) of various molecules and chemicals within cells. (huggingface.co)
Clejan, LA, Koop, DR & Cederbaum, AI 1989, ' Sensitivity of the rat liver microsomal oxidation of pyrazole to antibody raised against the ethanol-inducible rabbit liver cytochrome P-450 isozyme ', Drug Metabolism and Disposition , vol. 17, no. 6, pp. 694-698. (elsevierpure.com)
Furthermore, the beneficial effects of bicyclol were closely associated with the signaling pathways of bile acid metabolism (NPC1, SLCOLA4 and UGT1A1), cytochrome P450-mediated metabolism (CYP2C54, CYP3A11 and CYP3A25), biological processes such as metal ion metabolism (Ceruloplasmin and Metallothionein-1), angiogenesis (ALDH1A1) and immunological responses (IFI204 and IFIT3). (bvsalud.org)
Microsomes6
The primary metabolite of PHT, pHPPH, is generated via cytochrome P450, so initial experiments were conducted with human livermicrosomes. (aspetjournals.org)
Zhou, Q., Zhou, S. and Chan, E. (2005) Effect of Omeprazole on the Hydroxylation of Warfarin Enantiomers in Human: In-Vitro Studies with LiverMicrosomes and cDNA-Expressed Cytochrome P450 Isozymes. (scirp.org)
Moreover, midazolam hydroxylase activity was immunoprecipitated from solubilized human microsomes with polyclonal antibodies against rat P450PCN1 and the closely related human isozyme P450NF. (aspetjournals.org)
Two cholesterol 7 alpha-hydroxylase isozymes were purified from livermicrosomes of cholestyramine-treated female rats by using anion exchange high performance liquid chromatography. (omeka.net)
Pyrazole is oxidized to 4-hydroxypyrazole by rat livermicrosomes in a cytochrome P-450-dependent reaction and this oxidation can be increased by prior treatment of rats with pyrazole, 4-methylpyrazole, or chronic ethanol feeding. (elsevierpure.com)
Hepatic2
Objectives: The objective of this study was to explore potential drug-drug/food interactions of ciprofloxacin and grapefruit juice, known hepatic cytochrome P450 (CYP) 1A2 inhibitors, on single-dose oral pharmacokinetics of riluzole, a substrate of CYP 1A2 enzymes. (researchgate.net)
Despite the evidence, whether and how the female hormones modulate expression and/or activities of major hepatic P450 enzymes remains unclear. (aspetjournals.org)
CYP2D61
Modeling chemical interaction profiles: I. Spectral data-activity relationship and structure-activity relationship models for inhibitors and non-inhibitors of cytochrome P450 CYP3A4 and CYP2D6 isozymes. (cdc.gov)
Liver6
Selective inactivation by chloramphenicol of the major phenobarbital-inducible isozyme of dog liver cytochrome P-450. (aspetjournals.org)
Chloramphenicol (CAP) is a potent and effective mechanism-based inactivator of the major phenobarbital (PB)-inducible isozyme of dog liver cytochrome P-450 (PBD-2) in vitro. (aspetjournals.org)
A multicompartment geometric model of the liver in relation to regional induction of cytochrome P450s. (cdc.gov)
The formation of both metabolites was inhibited by more than 90% by an antiserum directed against a pregnenolone 16 alpha-carbonitrile-inducible cytochrome P450 (P450PCN1) of rat liver. (aspetjournals.org)
Metabolized in the liver via the cytochrome P450 oxidase isozyme CYP2C19. (rxmedworld.co)
Isoforms4
To this end, primary human hepatocytes were treated with estradiol and progesterone, and mRNA expression and activity levels of 10 different P450 isoforms were determined. (aspetjournals.org)
In this study, we aimed to clone brain-derived Cyp19b and ovary-derived Cyp19a the P450 aromatase gene isoforms and to indicate the expression levels of these genes in the hypothalamus and ovary tissues from reproductively arrested ovarian development (RA) and non-arrested ovarian development (RN) Alburnus tarichi from Lake Van, Turkey. (egejfas.org)
Distinct cytochrome P450 aromatase isoforms in the common carp (Cyprinus carpio): Sexual dimorphism and onset of ontogenic expression. (egejfas.org)
Oxidation2
Oxidation of ranitidine by isozymes of flavin-containing monooxygenase and cytochrome P450. (nih.gov)
Identification of the cytochrome P-450 isozymes responsible for testosterone oxidation in rat lung, kidney, and testis: evidence that cytochrome P-450a (P450IIA1) is the physiologically important testosterone 7 alpha-hydroxylase in rat testis. (wikigenes.org)
Genes2
Physical mapping of overlapping cosmid and phage isolates defined two genes (designated Cyp11b-1 and Cyp11b-2 in the standard nomenclature for cytochrome P450 genes) that are oriented in the same direction and separated by approximately 8 kilobase pairs of DNA. (elsevierpure.com)
Two Cyp19 (P450 Aromatase) genes on duplicated Zebrafish chromosomes are expressed in ovary or brain. (egejfas.org)
Humans3
Named officially the cytochrome P450 11B1, mitochondrial, it is a protein that in humans is encoded by the CYP11B1 gene. (wikipedia.org)
Humans have two isozymes with 11β-hydroxylase activity: CYP11B1 and CYP11B2. (wikipedia.org)
In humans, cytochrome P450 1A2 is the principal isozyme involved in N-hydroxylation. (nih.gov)
Enzymes1
The CYP11B1 gene encodes 11β-hydroxylase - a member of the cytochrome P450 superfamily of enzymes. (wikipedia.org)
Pathways1
Signal pathway analysis revealed that TGF- β 1 impaired the pathways of cell cycle and cytochrome P450 detoxification, and EGF reverted TGF- β 1 effects through activating MAPK and PI3K-Akt pathway. (hindawi.com)
Antibody1
this P-450 isozyme is recognized by antibody (anti-3a IgG) raised against the ethanol-inducible P-450 in rabbits. (elsevierpure.com)
CYP11B21
The CYP11B1 isozyme has strong 11β-hydroxylase activity, but the activity of 18-hydroxylase is only one-tenth of CYP11B2. (wikipedia.org)
Reaction1
Note the extra "-OH" added at the 11 position (near the center, on ring "C"): 11-Deoxycortisol Cortisol 11-Deoxycorticosterone Corticosterone As a mitochondrial P450 system, P450c11 is dependent on two electron transfer proteins, adrenodoxin reductase and adrenodoxin that transfer 2 electrons from NADPH to the P450 for each monooxygenase reaction catalyzed by the enzyme. (wikipedia.org)
Activity5
In a reconstituted system containing PBD-2, CAP causes a time- and NADPH-dependent irreversible loss of 7-ethoxycoumarin deethylase activity, with no loss of spectrally detectable cytochrome P-450. (aspetjournals.org)
In addition to the 11β-hydroxylase activity, both isozymes have 18-hydroxylase activity. (wikipedia.org)
Using cytochrome P-450s expressed in a lymphoblastoid cell line in presence of epoxide hydrolase (EH), human 1A1 had approximately twice the activity of human 1A2. (nih.gov)
There is marked interindividual variability in the clearance of riluzole, probably attributable to variability of CYP 1A2 activity, the principal isozyme involved in N-hydroxylation. (nih.gov)
These directional changes in P450 activity are similar to those occurring during pregnancy. (aspetjournals.org)
Selective1
This selective expression of the mouse 11β-OHase isozymes in different adrenocortical zones very likely contributes significantly to its functional separation into glucocorticoid- and mineralocorticoid-producing compartments. (elsevierpure.com)
Expression1
We next studied the expression of these two isozymes in Y1 adrenocortical tumor cells and in the intact mouse adrenal gland. (elsevierpure.com)
Hepatic2
Selective protection of curcumin against carbon tetrachloride-induced inactivation of hepatic cytochrome P450 isozymes in rats. (therapeuticresearch.com)
HUREL Micro Livers consist of cryopreserved primary hepatocytes co-cultured with a stromal fibroblast cell line, which aids in the hepatocytes' maintenance of more in vivo -like characteristics compared to traditional hepatic mono-cultures, including long term viability and retention of cytochrome P450 isozyme activity. (visikol.com)
Vitro5
This study evaluated the in vitro inhibitory potential of the aqueous extract of H. sabdariffa calyces (AEHS) on selected cytochrome P450 (CYP) isozymes and the effect of HSB on the pharmacokinetics of caffeine in vivo. (nih.gov)
Drugs that are substrates or inhibitors of the same isozyme in vitro may be predicted to interact if taken concomitantly, but often the interaction is not detected, or may not be clinically significant, in vivo . (medicalletter.org)
Inhibition of cytochrome P450 isozymes by curcumins in vitro and in vivo. (therapeuticresearch.com)
His Cytochrome P450 research is multidisciplinary, relying on both Cytochrome, In vitro, Epoxide hydrolase and Isozyme. (research.com)
In vitro studies indicate that moxifloxacin does not inhibit CYP3A4, CYP2D6, CYP2C9, CYP2C19, or CYP1A2, indicating that moxifloxacin is unlikely to alter the pharmacokinetics of drugs metabolized by these cytochrome P450 isozymes. (descarga.nu)
Microsomal4
CdCl 2 -treated testes increased HO-1 activity and suppressed microsomal cytochromes P450, which are required for steroidogenesis. (jci.org)
Microsomal protein level, NADPH cytochrome c reductase activity, and the activities and protein levels of cytochrome P-450 isozymes CYP1A1 and CYP2B1 were monitored to assess the effects of AFC exposure on pulmonary P-450. (cdc.gov)
In microsomal fraction of cells, there is an oxidase enzymatic complex, whose central element is the cytochrome P450, able to eliminate certain harmful substances. (longdom.org)
In microsomal fraction of cells, there is an oxidase enzymatic complex, whose central element is the cytochrome P450, located in a structure involved in transport (endoplasmic reticulum) and also in mitochondria (energy-producing centers). (longdom.org)
CYP3A41
His research on Enzyme and Cytochrome P450 is centered around CYP3A4. (research.com)
Monooxygenase2
Cytochrome P-450 isozymes and monooxygenase activity in aquatic animals. (nih.gov)
Note the extra "-OH" added at the 11 position (near the center, on ring "C"): 11-Deoxycortisol Cortisol 11-Deoxycorticosterone Corticosterone As a mitochondrial P450 system, P450c11 is dependent on two electron transfer proteins, adrenodoxin reductase and adrenodoxin that transfer 2 electrons from NADPH to the P450 for each monooxygenase reaction catalyzed by the enzyme. (wikipedia.org)
Hydroxylation2
The Cytochrome P450 study combines topics in areas such as High-performance liquid chromatography, Methanol and Hydroxylation. (research.com)
The P450 OleTJE is a peroxygenase that binds long to medium chain fatty acids and converts them to a range of products originating from C-hydroxylation, C-hydroxylation, C‒C desaturation and decarboxylation. (manchester.ac.uk)
P450s1
however, in specific isozymes, H2O2 rather than O2 is used and these P450s act as peroxygenases. (manchester.ac.uk)
Mitochondrial1
Named officially the cytochrome P450 11B1, mitochondrial, it is a protein that in humans is encoded by the CYP11B1 gene. (wikipedia.org)
Inhibitors1
Fluorometric high-throughput screening for inhibitors of cytochrome P450. (research.com)
Superfamily1
The cytochrome superfamily consists of nearly 60 members in humans (Human Genome Project 2013), which may be expressed differently in individual cells when challenged by xenobiotics, leading to cellular heterogeneity that remains concealed in a bulk analysis. (biomedcentral.com)
Compounds1
In stage I of drug digestion, oxidoreductase compounds, for example, cytochrome P450 oxidases add polar or receptive gatherings into drugs. (vedantu.com)
NADPH1
The reactions required NADPH and were inhibited by CO/O2 (4:1, v/v). Biphasic double-reciprocal plots of MDMA, MDA and MDB oxidation suggested participation of more than one isozyme of cytochrome P450 in the reaction. (erowid.org)
Enzyme1
His scientific interests lie mostly in Biochemistry, Cytochrome P450, Enzyme, Microsome and Cell culture. (research.com)
Rats2
Rats were exposed to cadmium chloride (CdCl 2 ), a stressor causing oligozoospermia, and HO-1-induction was monitored by following HO isozyme expression. (jci.org)
The results showed that exposure of rats to AFC did not significantly affect total cytochrome P-450 content or cytochrome c reductase activity in the lung. (cdc.gov)
Enzymatic1
To gain further insights into the structure and reactivity of P450 OleTJE, we set up a range of large active site model complexes as well as full enzymatic structures and did a combination of density functional theory studies and quantum mechanics/molecular mechanics calculations. (manchester.ac.uk)
Synthesis1
Cytochrome P450: factors determining synthesis in strain D7 of S. cerevisiae . (unibo.it)
Drugs1
Many drugs are metabolized by cytochrome P450 isozymes. (medicalletter.org)
System3
Alteration of pulmonary cytochrome P-450 system: effects of asphalt fume condensate exposure. (cdc.gov)
These isozymes are named according to a standard system, e.g. (medicalletter.org)
The main action of P450 system is to introduce a functional group to foreign lipophilic compound, which deveins water-soluble and it excretion was facilitated. (longdom.org)
Specific1
The specific cytochrome isozymes involved in thehepatic elimination of the drug are undefined. (psychiatrist.com)
Major1
In subjects lacking the 2D6 isozyme, CYP2C19 and 1A2 are expected to be of major importance for elimination of nortriptyline. (aspetjournals.org)
Drug1
Potential drug-drug interactions can be predicted with knowledge of affinitiesfor various cytochrome P450 (CYP) isozymes. (psychiatrist.com)