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Pseudomonas aeruginosaCystic FibrosisPseudomonas InfectionsPseudomonasCystic Fibrosis Transmembrane Conductance RegulatorFibrosisAnti-Bacterial AgentsBacterial ProteinsDisease OutbreaksMicrobial Sensitivity TestsPseudomonas putidaPyocyanineBurkholderia cepaciaPseudomonas fluorescensAlginatesPseudomonas PhagesDNA, BacterialGenes, BacterialMolecular Sequence DataBurkholderia InfectionsTobramycinGene Expression Regulation, BacterialSputumADP Ribose TransferasesBiofilmsVirulence FactorsDrug Resistance, MicrobialElectrophoresis, Gel, Pulsed-FieldMutationCross InfectionVirulenceQuorum SensingGlucuronic AcidSequence Analysis, DNABacterial Typing TechniquesPyocinsDrug Resistance, Bacterialbeta-LactamasesAmino Acid SequenceCarbenicillinExotoxinsBacterial ToxinsPlasmidsCeftazidimeStaphylococcus aureusMolecular EpidemiologyDNA FingerprintingBase SequenceCulture MediaGram-Negative BacteriaDrug Resistance, Multiple, BacterialHexuronic AcidsPolymerase Chain ReactionImipenemEscherichia coliSpecies SpecificityLungBacteriaBacterial Outer Membrane Proteins4-ButyrolactoneGentamicinsColistinPhenotypePseudomonas syringaeSerotypingGenotypeBacterial AdhesionBurkholderia cepacia complexRandom Amplified Polymorphic DNA TechniqueCiprofloxacinPancreatic ElastaseRibotypingColony Count, MicrobialThienamycinsCholeraEye Infections, BacterialCarbapenemsAminoglycosidesPneumonia, BacterialPseudomonas VaccinesPhylogenyAmikacinCloning, MolecularAztreonamMice, Inbred CFTRAnti-Infective AgentsAzlocillinGenome, BacterialVibrio choleraeSweatBacteriophage TypingDNA Transposable ElementsPseudomonas stutzeriAzurinAcinetobacterClostridium difficileEnvironmental MicrobiologyStaphylococcal InfectionsBacteriophagesPhenazines
IsolatesIsolated from Patients with Cystic FibrosisInfections in cystic fibrosisPAO1AntibioticsPathogenTemperate bacteriophagesGenomePatients infected with unique strainsAntibioticBiofilmLiverpool Epidemic StrainBacterialBronchiectasisInfectionMRSABacteriaVirulenceAureus strainsAntimicrobialRespiratoryEradicationClonalGene2019TransmissibleGeneticPseudomonalCohortLung diseaseAirwayRegulatory networksGenesSpeciesClinicAcuteDynamicsPeopleOutcomesCommonGenomicSevereInteractionsUrinaryPatternsMolecular
Isolates17
  • Isolates were cultured from spontaneous sputum and identified as P. aeruginosa by routine biochemical methods and matrix-assisted laser desorption/ionisation-time-of-flight (MALDI-TOF) mass spectrometry. (ersjournals.com)
  • We observed disease-specific phage infectivity profiles and found that phages induced from bacteria isolated from more advanced disease infected broader ranges of P. aeruginosa isolates. (ncl.ac.uk)
  • Using a novel metagenomic approach, we identified eight groups of temperate phages that were mobilized from 94 clinical P. aeruginosa isolates. (ncl.ac.uk)
  • Ceftolozane/tazobactam is a relatively recently available β-lactam/β-lactamase inhibitor combination that has been shown to have preserved activity against difficult-to-treat P. aeruginosa isolates. (paganz.org)
  • Methods: P. aeruginosa isolates from CF adults aged 15 years and over in Tasmania were genotyped using random amplified polymorphic DNA (RAPD)-PCR and clonal strains confirmed with pulsed field gel electrophoresis. (edu.au)
  • Additionally, exploring the regulatory differences between CA-MRSA and other MRSA strains may aid our understanding of the increase in virulence observed amongst community-associated isolates. (biomedcentral.com)
  • ABSTRACT This study aimed to characterize Pseudomonas aeruginosa isolates in 2 intensive care units in Egypt and Saudi Arabia. (who.int)
  • P. aeruginosa isolates from patients' and staff hands and environmental samples were typed using antibiotyping and ERIC-PCR. (who.int)
  • MBLs have been reported worldwide among Pseudomonas aeruginosa isolates ( 2 ). (blogspot.com)
  • During 2009-2016, infections with IMP-19-producing P. aeruginosa isolates were detected in 7 patients in the Hematology Department of University Hospital François Mitterrand, a 1,600-bed hospital in Dijon, France. (blogspot.com)
  • The aim of this study was to perform a genotyping analysis of the Pseudomonas aeruginosa -resistant isolates by the multilocus sequence typing (MLST) method at the university teaching hospital in Iran. (advbiores.net)
  • Antimicrobial susceptibility was analyzed for P. aeruginosa isolates. (advbiores.net)
  • Phenotypic tests to detect the metallo-β-lactamase strains of P. aeruginosa were performed on imipenem-resistant (IMP res ) isolates. (advbiores.net)
  • Of 35 P. aeruginosa isolates, 71%, 45% and 45% of isolates were CAZ res , IMP res and multidrug resistant (MDR), respectively. (advbiores.net)
  • This study shows a high rate of MDR P. aeruginosa isolates at the university teaching hospital in Iran. (advbiores.net)
  • It seems MDR isolates of P. aeruginosa ST235 with unique resistance pattern disseminated in this hospital. (advbiores.net)
  • Because of these advantages, we used an MLST scheme to characterize the strains of P. aeruginosa isolated from the clinical isolates, which enabled us to compare our data with those in the database generated from various clinical and environmental P. aeruginosa strains obtained from various countries. (advbiores.net)
Isolated from Patients with Cystic Fibrosis2
  • Identification of Burkholderia and Uncommon Glucose Non-fermenting Gram-Negative Bacilli Isolated from Patients with Cystic Fibrosis using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). Plongla Rongpong et al. (cdc.gov)
  • Mixed temperate phage populations were chemically mobilized from individual P. aeruginosa, isolated from patients with cystic fibrosis (CF) or bronchiectasis (BR). (ncl.ac.uk)
Infections in cystic fibrosis2
PAO14
  • The complete sequence of the genome of P aeruginosa strain, PAO1, is noted for its large size and diverse metabolic capacity. (medscape.com)
  • Two strains (PAO1 and PA14) have been mainly used as models for studying virulence of P. aeruginosa . (biomedcentral.com)
  • This project explores the dynamics between Pseudomonas aeruginosa PAO1 and its phage, determining whether they confer an advantage in the CF lung. (microbiologyresearch.org)
  • Finally, we used a subset of the temperate phages to infect P. aeruginosa PAO1 and tested the resulting lysogens for their susceptibility to antibiotics. (ncl.ac.uk)
Antibiotics5
  • This project will exploit novel nitric oxide based therapies to improve the effectiveness of antibiotics against Pseudomonas aeruginosa biofilms. (southampton.ac.uk)
  • Background Transmissible Pseudomonas aeruginosa (Psa) strains in the cystic fibrosis (CF) community increase the risk of chronic infection which, in turn, confers a poor prognosis and is difficult to treat, often requiring the use of multiple toxic intravenous antibiotics. (bmj.com)
  • As insulin is to people with diabetes, so are regular, high doses of antibiotics to people who have cystic fibrosis. (cysticfibrosis.co.za)
  • P. aeruginosa has intrinsic resistance to many antibiotics and produces a variety of virulence factors. (advbiores.net)
  • The intrinsic resistance of P. aeruginosa to various antibiotics is generally due to its low outer membrane permeability, production of the inducible AmpC chromosomal β-lactamase and multidrug efflux pumps. (advbiores.net)
Pathogen8
  • Pseudomonas is a clinically significant and opportunistic pathogen, often causing nosocomial infections. (medscape.com)
  • Sputum DNA sequencing in cystic fibrosis: non-invasive access to the lung microbiome and to pathogen details. (cdc.gov)
  • IMPORTANCE Pseudomonas aeruginosa is a key opportunistic respiratory pathogen in patients with cystic fibrosis and non-cystic fibrosis bronchiectasis. (ncl.ac.uk)
  • Pseudomonas aeruginosa is the most common bacterial pathogen causing lung infections in people with cystic fibrosis and appropriate antibiotic therapy is vital. (essentialevidenceplus.com)
  • Pseudomonas aeruginosa is a biofilm-forming opportunistic pathogen which causes chronic infections in immunocompromised patients and leads to high mortality rate. (frontiersin.org)
  • The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients. (burkholderia.com)
  • Pseudomonas aeruginosa is an opportunistic pathogen that is commonly responsible for nosocomial infections. (advbiores.net)
  • Pseudomonas aeruginosa is an opportunistic pathogen that is commonly responsible for nosocomial infections, including pneumonia, bloodstream infection, urine infection and ocular infection. (advbiores.net)
Temperate bacteriophages1
Genome11
  • This system detects 13 single-nucleotide polymorphisms within conserved P. aeruginosa genes and the presence/absence of 38 variable genetic markers in the accessory genome. (ersjournals.com)
  • The genome annotation is continually updated, and the database functionality is being expanded to facilitate accelerated discovery of P aeruginosa drug targets and vaccine candidates. (medscape.com)
  • Whole genome sequencing reveals the emergence of a Pseudomonas aeruginosa shared strain sub-lineage among patients treated within a single cystic fibrosis centre. (cdc.gov)
  • Population Dynamics of Staphylococcus aureus in Cystic Fibrosis Patients To Determine Transmission Events by Use of Whole-Genome Sequencing. (cdc.gov)
  • Whole-Genome Sequencing and Epidemiological Analysis Do Not Provide Evidence for Cross-transmission of Mycobacterium abscessus in a Cohort of Pediatric Cystic Fibrosis Patients. (cdc.gov)
  • Because of the clinical importance of the spread of such strain among CF patients we decided to sequence the genome of one representative isolate (strain CF-Marseille) to compare this to the published genome sequences. (biomedcentral.com)
  • Phylogenetic analyses using Complete Genome Hybridization and Multi Locus VNTR Assay showed that CF-Marseille was closely related to strain Mu50, representing vancomycin-resistant S. aureus . (biomedcentral.com)
  • Analysis of CF-Marseille shows a similar core genome to that of previously sequenced MRSA strains but with a different genomic organization due to the presence of specific mobile genetic elements i.e. a new SCCmec type IV mosaic cassette that has integrated the pUB110 plasmid, and a new phage closely related to phiETA3. (biomedcentral.com)
  • Even though diverse strains of S. aureus have been extensively studied, and subjected to genome sequencing, the function of a large proportion of their genes remains unidentified. (biomedcentral.com)
  • Comparison of Whole Genome (wg-) and Core Genome (cg-) MLST (BioNumericsTM) Versus SNP Variant Calling for Epidemiological Investigation of Pseudomonas aeruginosa. (applied-maths.com)
  • Genome-based phylogeny and genomic comparisons based on average nucleotide identity confirmed the strain to be a novel species of Cohnella. (academic-accelerator.com)
Patients infected with unique strains2
  • It confirms that it is unnecessary to segregate patients infected with unique strains from those without Psa infection. (bmj.com)
  • Patients harbouring AES III had suffered significantly more exacerbations requiring hospitalisation during the 2 years prior to the study compared with patients infected with unique strains (P (edu.au)
Antibiotic3
  • AES III displayed increased multi-antibiotic resistance compared with other strains (P (edu.au)
  • In 2006 in Marseille, France, we have detected an atypical MRSA strain with a specific antibiotic susceptibility profile and a unique growth phenotype. (biomedcentral.com)
  • S. aureus has obtained notoriety in recent years due to the appearance and worldwide spread of antibiotic resistant strains. (biomedcentral.com)
Biofilm6
Liverpool Epidemic Strain4
  • We have a large cohort of patients infected with the Liverpool Epidemic Strain (LES), inherited from the paediatric sector, but no cohorts of other transmissible strains. (bmj.com)
  • The most important of these, the Liverpool Epidemic Strain (LES), is now widespread throughout UK CF clinics. (bmj.com)
  • The Liverpool Epidemic Strain(LES) of Pseudomonas aeruginosa is a key opportunistic bacterium and a major cause of mortality and morbidity in cystic fibrosis (CF) patients. (microbiologyresearch.org)
  • While the temperate phages of the Liverpool epidemic strain have been shown to be active in the human lung and enhance fitness in a rat lung infection model, little is known about their mobilization more broadly across P. aeruginosa in chronic respiratory infection. (ncl.ac.uk)
Bacterial3
  • The 3 stages of Pseudomonas infections are (1) bacterial attachment and colonization, (2) local infection, and (3) bloodstream dissemination and systemic disease. (medscape.com)
  • Pseudomonas aeruginosa is one of the most relevant human opportunistic bacterial pathogens. (biomedcentral.com)
  • Time between collection and storage significantly influences bacterial sequence composition in sputum samples from cystic fibrosis respiratory infections. (cdc.gov)
Bronchiectasis2
Infection13
  • Saiman L, Siegel J. Infection control recommendations for patients with cystic fibrosis: microbiology, important pathogens, and infection control practices to prevent patient-to-patient transmission. (cdc.gov)
  • Chronic infection of the respiratory tract is nearly universal in people with cystic fibrosis, with Pseudomonas aeruginosa, fungi, and mycobacteria all increasingly common over time. (wikipedia.org)
  • There are few data on P. aeruginosa cross-infection in NCFBr. (ersjournals.com)
  • Background Strategies to prevent chronic lung infection with Pseudomonas aeruginosa (Psa) in cystic fibrosis (CF) include early eradication and the prevention of cross-infection by patient segregation. (bmj.com)
  • However, cohorting patients into those with and without Psa infection does prevent superinfection by transmissible strains and is illogical since, by definition, unique strains pose no threat to non-Psa infected patients. (bmj.com)
  • In 2003 we introduced a rational cross-infection policy where those infected with transmissible strains are segregated from those with unique strains who are not separated from patients without chronic Psa infection. (bmj.com)
  • Results There was a decline in the proportion of patients with LES infection (from 71% to 56%) and an increase in those with unique strains (from 23% to 27%) and without Psa infection (from 8% to 33%), all due to a decrease in the proportion of Psa-infected patients from the paediatric sector ( table 1 ). (bmj.com)
  • In future, segregation policies should concentrate on preventing the spread of chronic infection with transmissible strains in the CF community. (bmj.com)
  • Cystic fibrosis (CF) is an inherited multi-system disorder characterised by chronic airway infection with pathogens such as Pseudomonas aeruginosa . (biomedcentral.com)
  • While it rarely infects healthy individuals, immunocompromised patients, like burn victims, AIDS-, cancer- or cystic fibrosis-patients are at increased risk for infection with this environmentally versatile bacteria. (up.ac.za)
  • This study was designed to identify the diverse communities of mobile P. aeruginosa phages by employing novel metagenomic methods, to determine cross infectivity, and to demonstrate the influence of phage infection on antimicrobial susceptibility. (ncl.ac.uk)
  • Whether such strains are more virulent or whether cross-infection between patients explains their widespread prevalence is unknown. (edu.au)
  • This study described the epidemiology of P. aeruginosa infection in CF patients in Tasmania, Australia, an area with a high CF birth incidence. (edu.au)
MRSA4
  • In this work, using diverse sequence analysis, we evaluated the repertoire of TFs and sigma factors in the community-associated methicillin resistant S. aureus (CA-MRSA) strain USA300-FPR3757. (biomedcentral.com)
  • In this work, we define the TF repertoire for the CA-MRSA strain USA300-FPR3757 and classify it into regulatory families. (biomedcentral.com)
  • All isolated S. aureus strains among the hospital staff nasal carriers were MRSA may influence the spread of bacteria and increase the percentage of persistent nosocomial infections in hospitalized patients. (ijpsr.com)
  • Variable SCC mec types are circulating within different countries, reflecting the various MRSA strains 2, 18 . (ijpsr.com)
Bacteria6
  • Discrepancy in MALDI-TOF MS identification of uncommon Gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis. (cdc.gov)
  • Bacteria belonging to the Pseudomonas group are common inhabitants of soil and water and can also be found on the surfaces of plants and animals. (up.ac.za)
  • Pseudomonas bacteria are renowned for their metabolic versatility as they can grow under a variety of growth conditions and do not need any organic growth factors. (up.ac.za)
  • In our hospital, P. aeruginosa is one of the top coinfecting bacteria identified among COVID-19 patients. (frontiersin.org)
  • has been identified as the 2nd most consensus (ERIC) sequences which · 60 environmental samples were tak- frequent organism causing ventilator- are common to Gram-negative enteric en throughout the ICU, including associated pneumonia, the 4th most bacteria [11,12]. (who.int)
  • Staphylococcus aureus is a bacteria that causes a heavy epidemic disease burden worldwide. (ijpsr.com)
Virulence2
  • In 1882, Gessard first discovered Pseudomonas , a strictly aerobic, gram-negative bacterium of relatively low virulence. (medscape.com)
  • Finally, we examine the conservation of 135 USA300 TFs amongst 11 other S. aureus strains, identifying a key group of regulators that display a high degree of conservation, including many that have previously been demonstrated to play a role in virulence gene regulation. (biomedcentral.com)
Aureus strains1
  • The analysis presented herein highlights the complexity of regulatory networks in S. aureus strains, identifies key conserved TFs among the Staphylococacceae , and offers unique insights into several as yet uncharacterized TFs. (biomedcentral.com)
Antimicrobial2
  • thus, efflux pump inhibitors are thought to be useful in reducing the invasiveness and antimicrobial resistance of P aeruginosa and may be promising as new anti-infectious agents. (medscape.com)
  • Children with cystic fibrosis are infected with multiple subpopulations of Mycobacterium abscessus with different antimicrobial resistance profiles. (cdc.gov)
Respiratory2
  • The primary cause of morbidity and death in people with cystic fibrosis is progressive lung disease, which eventually leads to respiratory failure. (wikipedia.org)
  • Several studies have shown that some rare respiratory diseases, such as alpha-1 antitrypsin deficiency (AATD), idiopathic pulmonary fibrosis (IPF), cystic fibrosis (CF), and primary ciliary dyskinesia (PCD) present oxidative stress (OS) and endoplasmic reticulum (ER) stress. (bvsalud.org)
Eradication1
  • Also note, this protocol demonstrated successful eradication of three different Bcc strains, some of which have not been previously associated with outcomes similar to an epidemic strain. (medscape.com)
Clonal3
  • Background and objective: Clonal strains of Pseudomonas aeruginosa have been identified in large cystic fibrosis (CF) centres. (edu.au)
  • Conclusions: Clonal strains of P. aeruginosa may arise even in isolated CF populations. (edu.au)
  • [2] The choice of seven loci ensures adequate variability in distinguishing the most closely related strains and still be able to track the global clonal history of the species with the highest possible accuracy. (advbiores.net)
Gene1
  • It is caused by the presence of mutations in both copies (alleles) of the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR) protein. (wikipedia.org)
20191
  • Outbreak of Pandoraea commovens Infections among Non-Cystic Fibrosis Intensive Care Patients, Germany, 2019-2021. (cdc.gov)
Transmissible3
  • Conclusions This study demonstrates that our rational segregation policy has controlled the spread of the commonest highly transmissible strain in the UK (LES) in our clinic, without endangering patients who were not previously infected with Psa. (bmj.com)
  • Our clinic regularly checks Psa genotypes and we have no cohorts of patients harbouring other transmissible strains. (bmj.com)
  • Anthroponoses (Greek "anthrópos" = saprophytically and replicate in these borne diseases (urban yellow fever, man, "nosos" = disease) are diseases substrata (i.e., not only to survive or con- dengue, epidemic typhus, tickborne transmissible from human to human. (cdc.gov)
Genetic1
  • Cystic fibrosis (CF) is a rare genetic disorder that affects mostly the lungs, but also the pancreas, liver, kidneys, and intestine. (wikipedia.org)
Pseudomonal1
  • Pseudomonas aeruginosa colonization reportedly occurs in more than 50% of humans, and P aeruginosa is the most common pseudomonal species. (medscape.com)
Cohort2
  • To look at this further, we compared such patterns of Psa strains in our CF clinic over 5 years, where we have a large cohort of patients infected with LES. (bmj.com)
  • Cross-transmission is not the source of new Mycobacterium abscessus infections in a multi-centre cohort of cystic fibrosis patients. (cdc.gov)
Lung disease1
  • Rapid detection of emerging pathogens and the loss of microbial diversity associated with severe lung disease in cystic fibrosis. (cdc.gov)
Airway1
Regulatory networks1
  • Production of these products is likely controlled by complex regulatory networks making Pseudomonas aeruginosa adaptable both to free-living and pathogenic lifestyles. (up.ac.za)
Genes1
  • Multilocus sequence typing (MLST) is a strain-typing system that focuses strictly on conserved housekeeping genes. (advbiores.net)
Species1
  • Whole genomic comparison between strain SM2-FT and close relatives suggested a novel species of a novel genus. (academic-accelerator.com)
Clinic2
Acute4
Dynamics1
People2
Outcomes1
  • In CF, epidemic infections with specific clones of P. aeruginosa are associated with further adverse outcomes [ 3 , 4 ]. (ersjournals.com)
Common2
  • Although P aeruginosa is a common human saprophyte, it rarely causes disease in healthy persons. (medscape.com)
  • cystic duct Cystic duct The duct that is connected to the gallbladder and allows the emptying of bile into the common bile duct. (lecturio.com)
Genomic4
  • Upon genomic comparisons of both strains, all values were below thresholds established for differentiation: average nucleotide identity (ANI, 88. (academic-accelerator.com)
  • Draft genomic comparison between the two strains revealed an average nucleotide identity of 99. (academic-accelerator.com)
  • In silico genomic comparisons confirm these observations and show that strain H1T shares 70. (academic-accelerator.com)
  • DNA-DNA hybridization and whole genomic comparison, mandatory to taxonomically separate strain 924/12T from the type strain of L. (academic-accelerator.com)
Severe1
  • Severe scarring and loss of lung tissue can ultimately strain the right side of the heart as it tries to pump blood through the altered lung tissue. (msdmanuals.com)
Interactions1
Urinary1
Patterns1
  • P. aeruginosa was isolated from 34 patients and nine (26%) of these individuals harboured P. aeruginosa strains with identical RAPD-PCR and pulsed field gel electrophoresis patterns (Australian Epidemic Strain III - AES III). (edu.au)
Molecular1
  • Molecular Epidemiologic Investigation of Mycobacterium intracellulare subspecies chimaera Lung Infections at an Adult Cystic Fibrosis Program. (cdc.gov)