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  • mutation
  • PAO affinity pull-down and mutation of individual cysteine to alanine reveal that Cys235, Cys311, Cys316, Cys414, and Cys506 are critical for binding, and binding is modulated by intramolecular interactions. (cdc.gov)
  • Mutation analyses of the two cysteines in human MRP1 revealed that the Cys(7) residue is critical for the MRP1-mediated drug resistance and leukotriene C(4) transport activity. (nih.gov)
  • However, the capacity of charged sulfhydryl reagents to inhibit the binding of NBMPR in broken cell preparations (allowing cytoplasmic access) was not affected by mutation of any of the cysteines (Cys87, 193,213, 222) in the N-terminal half of the protein. (aspetjournals.org)
  • Mutation of cysteine residues 212, 382, and 422 had little to no effect upon cell surface expression or fusion activity at 32°C, 37°C, or 39.5°C. Mutation of C37 and C69 in the F2 subunit either abolished or reduced cell surface expression by 75% respectively. (biomedcentral.com)
  • Mutation of a leucine residue in the third coiled-coil heptad impaired CWMV CRP activity for suppression of local silencing, but not for the promotion of cell-to-cell movement of Potato virus X (PVX). (biomedsearch.com)
  • Deletion of the C-terminal cysteine cluster or mutation of the cysteine residues abolishes or markedly reduces the transcription activation activity of MTF1 and the ability of MTF1 to restore Mt1 induction in Mtf1 knockout cells. (cdc.gov)
  • Mutation of the three charged polar residues Asp 45 , Lys 48 and Asp 62 had the greatest deleterious effect, suggesting that electrostatic interactions between TWEAK and Fn14 residues may be particularly important for complex formation or stability. (biochemj.org)
  • catalytic
  • These individual subunits were analysed for their sensitivity to N -ethylmaleimide (NEM), in order to assess the roles that cysteine residues play in the partial reactions that comprise the catalytic cycle of Mg 2+ chelatase, such as the ATPase activity of ChlI, and the formation of ChlI-ChlD-MgATP and ChlH-protoporphyrin complexes. (biochemj.org)
  • Glavas S & Tanner ME (1999) Catalytic acid/base residues of glutamate racemase. (warwick.ac.uk)
  • protein
  • p. 449: "The mutated protein, purified from porin-lacking yeast mitochondria, showed channel conductance and voltage-dependence similar to that of native rVDAC1, following reconstitution into a PLB ( Figures 3 E and 3 F)." Thus in the next sentence ["As expected, the channel conductance of the cysteine-less rVDAC1 was not reduced by 5-FM (results not shown)"], the data are now presented ( Figures 3 E and 3 F). (biochemj.org)
  • NRF2 cysteine residues are critical for oxidant/electrophile-sensing, Kelch-like ECH-associated protein-1-dependent ubiquitination-proteasomal degradation, and transcription activation. (cdc.gov)
  • We identified multiple receptor-like protein kinases changing in abundance, including cysteine (Cys)-rich receptor-like kinases (CRKs) that are up-regulated upon the perception of flagellin. (plantphysiol.org)
  • We demonstrate here that the enzyme can complement a bacterial strain which is dependent upon methionine sulfoxide reduction for growth, that the purified recombinant protein is enzymatically active showing stereospecificity towards R -methionine sulfoxide, and identify the active site and two resolving cysteine residues. (biochemj.org)
  • Here, we present evidence that the conserved cysteine residues and Cys-Gly-X-X-His motif in the CRP of Chinese wheat mosaic virus (CWMV) are critical for protein stability and silencing suppression activity. (biomedsearch.com)
  • thiols
  • Quantitative analysis of the effects of NEM binding on ChlI-catalysed ATPase activity showed that three out of four thiols per ChlI molecule are available to react with NEM, but only one cysteine residue per ChlI subunit is essential for ATPase activity. (biochemj.org)
  • ATPase
  • Neither the ATPase activity of ChlI nor NEM-modifiable cysteines are therefore required to form the ChlI-ChlD-MgATP complex. (biochemj.org)
  • peptides
  • Background: Conotoxins are neuro-pharmacologically active cysteine rich peptides isolated from the venom complex of marine cone snails. (iisc.ac.in)
  • inducers
  • Preincubation with As(3+), Cd(2+), Co(2+), Ni(2+), Ag(+), Hg(2+), and Bi(3+) blocks pulldown of MTF1(321-675) by PAO beads in vitro and in vivo, indicating that binding of the metal inducers to the same C-terminal cysteine cluster as PAO occurs. (cdc.gov)
  • Biochemistry
  • It has been suggested that a cysteine residue could fulfil the role of the active site nucleophile {[}Hardy and Poteete (1991) Biochemistry 30, 9457- (ubc.ca)
  • membrane
  • A previous study from our laboratory established Cys222 in TM6 as the residue responsible for MMTS (membrane-permeable sulfhydryl modifier)-mediated enhancement of the binding of the ENT1 inhibitor nitrobenzylthioinosine (NBMPR) in intact cells. (aspetjournals.org)
  • Redox-active cysteines of a membrane electron transporter DsbD show dual compartment accessibility. (semanticscholar.org)
  • active
  • Thus we have directly identified a reactive cysteine residue as part of the active site, i.e. the substrate-binding domain, of Ins(1,4,5) P 3 5-phosphatase. (biochemj.org)
  • On the basis of these results it seems unlikely that the role of the active-site nucleophile in retaining glycosidases could successfully be filled by a cysteine residue. (ubc.ca)
  • We conclude that thimerosal selectively sensitizes IP(3)R1 and IP(3)R2 to IP(3) by modifying cysteine residues within both the SD and IBC and thereby stabilizing an active conformation of the receptor. (ox.ac.uk)
  • critical
  • Our results indicate that three residues within the predicted Fn14 CRD A1 module (Asp 45 , Lys 48 and Met 50 ) and one residue within the predicted D2 module (Asp 62 ) are each critical for high-affinity TWEAK binding. (biochemj.org)
  • To determine whether the four critical residues were likely to be located on the Fn14 CRD surface, we made an Fn14 homology model based on a previously derived X-ray structure for the B-cell maturation antigen receptor, which also contains only one CRD. (biochemj.org)
  • This model revealed that each of these critical residues were in areas of the receptor that are potentially capable of interacting with TWEAK. (biochemj.org)
  • Cys414
  • We thus hypothesized that the inhibitory effects of the modifiers were due to the one or more of the six cysteine residues in the C-terminal half of ENT1, particularly one or both of those in the fifth intracellular loop (Cys414 and Cys416). (aspetjournals.org)
  • near
  • PAO affinity beads effectively pulls down the carboxyl half of MTF1 (MTF1(321-675)) by binding to a cluster of five cysteine residues near the terminus. (cdc.gov)
  • contains
  • The 8151 nucleotide open reading frame of the A gene contains several tandem repeats of 210 nucleotides within the central portion of the molecule as well as a periodic structure defined by cysteine residues. (mysciencework.com)