Loading...
  • parasites
  • Binding assays performed by probing living parasites with fluorescein (FITC)-cruzipain or FITC-chagasin revealed the presence of both inhibitor and protease at the cell surface of amastigotes. (lu.se)
  • Calpains
  • A BODIPY Fluorescent Microplate Assay for Measuring Activity of Calpains and Other Proteases," Analytical Biochemistry, 279, (2000), pp. 170-178. (patentgenius.com)
  • putative
  • Three putative protease targets (termed cryptopains 1 to 3, or CpaCATL-1, -2, and -3) were identified in the C. parvum genome, but only two are transcribed in infected mammals. (asm.org)
  • recombinant
  • Using AEP-mediated cleavage sequences, we modeled the effects of the protease on ASNase and created a number of recombinant ASNase products. (jci.org)
  • Tobacco
  • In our previous report, the targeted expression of Arachis cysteine protease in tapetum led to premature degeneration of tapetal layer that resulted in complete male sterility in transgenic tobacco plants. (springer.com)
  • intracellular
  • Activation occurs once the protease is delivered to a specific intracellular compartment (e.g. lysosome ) or extracellular environment (e.g. stomach ). (wikipedia.org)
  • amino acid
  • 3. An inhibitor of claim 2 wherein the cysteine protease binding moiety comprises (i) amino acids, (ii) amino acids and amino acid mimetics, or (iii) amino acid mimetics, wherein the moiety corresponds to a peptide of 5 amino acid residues. (patentgenius.com)
  • It is speculated that domains 2 and 3 contain two regions as potential candidates for the reactive site of cysteine proteases : Gln-Val-Val-Ala-Gly (QVVAG) located at approximately two-thirds of the distance from the N-terminus of the domains and Gly located at 44 amino acid residues upstream from the QVVAG region. (nii.ac.jp)
  • diseases
  • The ability of macrophages and other cells to mobilize elastolytic cysteine proteases to their surfaces under specialized conditions may also lead to accelerated collagen and elastin degradation at sites of inflammation in diseases such as atherosclerosis and emphysema . (wikipedia.org)
  • The compounds and compositions are useful in methods of treating or preventing one or more diseases associated with cysteine protease activity, particularly those associated with calpain activity. (patentgenius.com)
  • residue
  • In this step, a fragment of the substrate is released with an amine terminus, the histidine residue in the protease is restored to its deprotonated form, and a thioester intermediate linking the new carboxy-terminus of the substrate to the cysteine thiol is formed. (wikipedia.org)
  • sequence
  • These proteases are similar in sequence (68% identity between falcipain-2 and falcipain-3) and appear to function as food vacuole hemoglobinases ( 4 , 5 ), as was recently confirmed for falcipain-2 ( 6 ). (pnas.org)