GlomerularEGFRCysCSuperfamilyBased on serum cystatinInhibitorRole of cystatinExpression of cystatinProportion of cystatinAmyloidConcentrationsCST3Human cystatinCerebral hemorrhageProteinELISAEquationsCystatinsSerum creatinineAntibodyCoronaryUrineLaboratoriesProteinsCreatinineChronicConcentrationCross-reactivitySpecificityMarkerEquationAssaysEstimateClinicalAssayChickenKidney functionLevel
Glomerular14
- Serum cystatin C has been used to estimate glomerular filtration rate to define the presence of CKD," write Ronald Klein, MD, MPH, from the University of Wisconsin School of Medicine and Public Health, Madison, and colleagues. (medscape.com)
- Casual blood specimens were obtained at each examination to determine white blood cell count, serum blood urea nitrogen, glomerular filtration rate, and cystatin C. The glomerular filtration rate from serum creatinine was estimated using the Modification of Diet in Renal Disease (Study) prediction equation. (medscape.com)
- The goal of this update is to raise awareness of clinical scenarios where cystatin C has clear and immediate benefits as an alternative glomerular filtration rate (GFR) biomarker to supplement creatinine. (lww.com)
- A cystatin C (cys)-based European Kidney Function Consortium (EKFC) equation has similar accuracy to the creatinine (cr)-based equation for estimating glomerular filtration rate (GFR), according to a study published in the Jan. 26 issue of the New England Journal of Medicine . (medicalxpress.com)
- For this reason in this study, the glomerular filtration rate (GFR) of 30 dogs of different breeds with a broad range of bodyweight were evaluated by using serum cystatin C to determine its usefulness as a marker. (vin.com)
- In this editorial, the simultaneous use of creatinine and cystatin C to estimate GFR, muscle mass and selective glomerular hypofiltration syndromes is described. (lu.se)
- Glomerular filtration in the kidneys removes Cystatin C from the blood plasma. (arborassays.com)
- A linear relationship exists between the reciprocal Cystatin C concentration in plasma and the glomerular filtration rate (GFR). (arborassays.com)
- The cystatin-c test analyses the working of the kidney by measuring the glomerular filtration rate. (redcliffelabs.com)
- Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. (betalifesci.com)
- Indeed, the concentration of Cystatin C is mainly determined by glomerular filtration and is particularly of interest in clinical settings where the relationship between creatinine production and muscle mass impairs the clinical performance of creatinine. (betalifesci.com)
- Inker et al reported equations for estimating glomerular filtration rate (eGFR) based on serum cystatin C concentrations, age and gender. (medicalalgorithms.com)
- Comparison of Cystatin C and Creatinine-Based Equations with Measured Glomerular Filtration Rate in a Diverse Pediatric Population. (bvsalud.org)
- While measured glomerular filtration rate (mGFR) is occasionally used as a reference, estimated GFR (eGFR) from serum creatinine - and cystatin C (CysC)-based equations are routinely used in clinical practice as a reliable and less invasive approach. (bvsalud.org)
EGFR2
- Furthermore, cystatin C offers the opportunity to avoid the race coefficient that is required for any current creatinine-based eGFR equation, which has been appropriately criticized for introducing unnecessary imprecision, assumptions and values on GFR estimation. (lww.com)
- Mean (standard deviation) molybdenum-corrected urine cadmium , Modification of Diet in Renal Disease (MDRD) eGFR and multi-variable cystatin C eGFR were 1.02 (0.65) ug/g creatinine, and 97.4 (19.2) and 112.0 (17.7) mL/min/1.73 m2, respectively. (cdc.gov)
CysC2
- Because it has been proven that in advanced liver diseases or liver cirrhosis an overestimation of the GFR occurs when sCr is used, the measurement of serum cystatin C (CysC) was recommended to detect a possibly impaired renal function. (aacc.org)
- In the October 23 Neuron, Gan and colleagues now report that APP mice lacking cystatin C (CysC)-an inhibitor of cysteine proteases including CatB-have lower soluble Aβ levels and reduced Aβ-associated deficits in cognition, behavior, and synaptic plasticity compared to APP/CysC+/+ mice. (alzforum.org)
Superfamily3
- The amount of any cystatin (that is, of any member of the cystatin superfamily of proteins, many of which act as cysteine protease inhibitors) found in a specified sample of blood, the fluid that circulates through the heart, arteries, capillaries and veins carrying nutrients and oxygen to the body tissues and metabolites away from them. (mcw.edu)
- Cystatin A, also known as Stefin A, Cystatin AS or Keratolinin, is a member of family 1 of the cystatin superfamily. (reliatech.de)
- Cystatin C is a low molecular weight non-glycosylated protein (13 kDa) in the cystatin superfamily. (arborassays.com)
Based on serum cystatin1
- We compared associations of urine cadmium with kidney function measures based on serum cystatin C to those with serum creatinine in 712 lead workers. (cdc.gov)
Inhibitor8
- Zurdel et al chose as a potential candidate the cystatin C gene- CST3 -coding for a protease inhibitor common in tissues and body fluids which strongly inhibits cathepsins, including cathepsin S, and results in debris accumulating around retinal pigment epithelial cells. (bmj.com)
- Cystatin C belongs to the cysteine proteinase inhibitor group. (arborassays.com)
- To analyze the contribution of cysteine proteases to metastasis we have over-expressed in B16 melanoma cells the natural cysteine protease inhibitor, cystatin C. We measured in vitro invasion of cystatin over-expression clones with Boyden chamber type assays. (biomedcentral.com)
- Cystatin C is a type II cysteine protease inhibitor that is normally secreted from cells [ 10 ]. (biomedcentral.com)
- Cystatin C is an inhibitor of cathepsins. (eucys2017.eu)
- Cystatin C, a cysteine protease inhibitor, was subject to hydrolysis at two sites when complexed with papain and in the presence of excess papain. (mcmaster.ca)
- A pH-dependent conformational variability in this region of the inhibitor could explain the differences in the X-ray crystallographic and n.m.r. structures of the homologous chicken cystatin. (mcmaster.ca)
- Cystatin A (Cys A), a cysteine protease inhibitor, is a precursor of proteins involves in keratinocyte keratinization, and is expressed during the late phase of differentiation of these cells. (bvsalud.org)
Role of cystatin2
- We used the common variant rs911119 in CST3 as an instrumental variable to investigate the causal role of cystatin C in CVD, including coronary heart disease, ischemic stroke, and heart failure. (scilifelab.se)
- Mendelian randomization analyses did not support a causal role of cystatin C in the etiology of CVD. (scilifelab.se)
Expression of cystatin3
- In this study we show over-expression of cystatin C in melanoma cells is associated with reduced metastasis and increased apoptosis in lung tissues. (biomedcentral.com)
- The expression of cystatin C-GFP fusion was observed in B16F10 melanoma cells following transient transfection of plasmid construct DNA. (biomedcentral.com)
- Studies suggest that expression of cystatin A is inversely associated with malignant progression of cancer 9 . (bvsalud.org)
Proportion of cystatin1
- This indicates that at physiological ionic strength of 0.15 M, a significant proportion of cystatin C complexed with protease would be in a proteolytically labile conformation over the pH range 4.5 to 5, which is encountered in lysosomes. (mcmaster.ca)
Amyloid5
- In light of prior human polymorphism data and recent mouse data highlighting cystatin C's ability to interfere with amyloid aggregation, the new findings suggest a more complex character for cystatin C and make it challenging to say whether it plays a "good" or "bad" role in AD pathogenesis. (alzforum.org)
- At first glance, this trend would seem to back recent work indicating a seemingly beneficial role for cystatin C in AD mice-as an agent that interferes with amyloid aggregation (see ARF related news story ). (alzforum.org)
- The imbalance between Cystatin C and cysteine proteinases is associated with conditions such as cancer, Alzheimer's disease, multiple sclerosis, and hereditary Cystatin C amyloid angiopathy. (arborassays.com)
- Scholars@Duke publication: Cystatin C mutation in an elderly man with sporadic amyloid angiopathy and intracerebral hemorrhage. (duke.edu)
- BACKGROUND: Cerebral amyloid angiopathy (CAA) with intracerebral hemorrhage (ICH) occurs both sporadically and as a result of mutations in either cystatin C or the amyloid precursor protein. (duke.edu)
Concentrations4
- citation needed] The cystatin family includes: The Type 1 cystatins, which are intracellular and are present in the cytosol of many cell types, but can also appear in body fluids at significant concentrations. (wikipedia.org)
- vancomycin was the most frequently studied drug and its target level and elimination were better predicted by cystatin C. Overall, approaches to medication dosing and monitoring that include cystatin C concentrations have been shown to result in a better achievement of drug trough levels. (lww.com)
- Serum cystatin C concentrations (sCysC) might be less influenced by changes in body muscle mass and so better indicate the presence of concurrent chronic kidney disease (CKD) in hyperthyroidism. (avmi.net)
- ELISA) was used to determine the concentrations of serum pentraxin-3 and cystatin C. Utilizing SPSS version 22, statistical analysis was conducted. (pafmj.org)
CST31
- The CST3 BB genotype and low cystatin C cerebrospinal fluid levels are associated with dementia in Lewy body disease. (nih.gov)
Human cystatin11
- Product Description google Human Cystatin C ELISA Kit (DIY Antibody Pairs) (antibody pairs and standards for assay development). (biosensis.com)
- Specificity This human Cystatin C ELISA assay is reactive to cystatin C in human samples. (biosensis.com)
- The Cystatin C Human ELISA Kit quantitatively measures Human Cystatin C levels in serum, plasma (EDTA and Heparin), urine, and tissue culture media. (arborassays.com)
- Use our provided human Cystatin C standard to generate a standard curve for the assay. (arborassays.com)
- Pipette the standards or diluted samples into a transparent microtiter plate coated with our mouse anti-human Cystatin C antibody and incubate at room temperature for 1 hour. (arborassays.com)
- Add the peroxidase-conjugated human Cystatin C monoclonal antibody. (arborassays.com)
- The mature, active form of human cystatin C is a single non-glycosylated polypeptide chain consisting of 12 amino acid residues, with a molecular mass of 13,343-13,359 Da, and containing four characteristic disulfide-paired cysteine residues. (betalifesci.com)
- A DNA sequence encoding the human Cystatin-C (NP_000090.1) (Ser 27-Ala 146) was expressed with a C-terminal His tag. (betalifesci.com)
- The mature recombinant human Cystatin-C consists of 131 a.a. and predicts a molecular mass of 14.8 kDa. (betalifesci.com)
- Description: A competitive ELISA for quantitative measurement of Human Cystatin SN(CST1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (cromauv.org)
- Evaluate the levels of serum pentraxin-3 and human cystatin C in migraine patients and the relationship between various parameters in migraine patients to healthy controls. (pafmj.org)
Cerebral hemorrhage1
- CASE DESCRIPTION: We report a case of sporadic CAA with ICH in an elderly Croatian man with a mutation in cystatin C identical to that found in Icelandic hereditary cerebral hemorrhage with amyloidosis. (duke.edu)
Protein9
- Cystatin: a protein that flips out! (wikipedia.org)
- Cystatin C, β-trace protein (BTP) and β2 microglobulin (B2M) assayed from stored surplus serum samples from the NHANES III Second Examination (1988-1994). (cdc.gov)
- To help establish mechanism of cystatin C action in metastasis we have fused cystatin C to the N-terminus of green fluorescent protein. (biomedcentral.com)
- Confocal microscopy was used in an attempt to localize cystatin C-GFP protein expression in cells. (biomedcentral.com)
- This pattern of expression was not anticipated since cystatin C is primarily a secreted protein that should have produced a vesicular localization of fluorescence within the cell. (biomedcentral.com)
- Figure 2 shows expression of the cystatin C-GFP fusion protein. (biomedcentral.com)
- Cystatin C is a low-molecular-weight protein which has been proposed as a marker of renal function that could replace creatinine. (betalifesci.com)
- In almost all the clinical studies, Cystatin C demonstrated a better diagnostic accuracy than serum creatinine in discriminating normal from impaired kidney function, but controversial results have been obtained by comparing this protein with other indices of kidney disease, especially serum creatinine-based equations, such as early atherosclerosis, Alzheimer's dementia, vascular aneurysms, hyperhomocysteinaemia and other neurodegenerative diseases. (betalifesci.com)
- Cystatin C is a protein that is produced by the cells in your body. (kidney.org)
ELISA2
- The Cystatin C Human ELISA Kit is a sandwich ELISA with a run time of 2 hours. (arborassays.com)
- Description: A competitive ELISA for quantitative measurement of Rat Cystatin SN(CST1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (cromauv.org)
Equations1
- Equations that include cystatin C predict GFR more accurately than serum creatinine in children, adults, and older adults with larger effects among persons who are acutely ill. (lww.com)
Cystatins2
- Cystatins show similarity to fetuins, kininogens, histidine-rich glycoproteins and cystatin-related proteins. (wikipedia.org)
- These are cystatin-like proteins found in a range of organisms: plant phytocystatins, fetuin in mammals, insect cystatins, and a puff adder venom cystatin, which inhibits metalloproteases of the MEROPS peptidase family M12 (astacin/adamalysin). (wikipedia.org)
Serum creatinine4
- Rescaled serum creatinine in the EKFC eGFRcr equation was replaced with rescaled cystatin C, and the resulting EKFC eGFRcys equation was validated in cohorts of White and Black patients in Europe, the United States, and Africa. (medicalxpress.com)
- Cystatin C serum concentration is not affected by age, gender, or body mass, suggesting it may be a better marker for GFR than serum creatinine. (arborassays.com)
- Differences in urine cadmium associations with kidney outcomes based on serum creatinine and cystatin C. (cdc.gov)
- Urine creatinine was associated with serum creatinine-based but not cystatin-C-based eGFRs. (cdc.gov)
Antibody3
- This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse) immunized with human recombinant Cystatin-A. (reliatech.de)
- After the 30-minute incubation, wash away the excess peroxidase-conjugated Cystatin C monoclonal antibody and add the TMB substrate. (arborassays.com)
- The TMB substrate reacts with the bound peroxidase-conjugated Cystatin C monoclonal antibody generating a signal detected by a plate reader at 450nm. (arborassays.com)
Coronary1
Urine1
- Cystatin C is freely filtered in the renal glomeruli and is normally re-absorbed in the proximal tubules where it is nearly completely metabolised, leading to insignificant levels in the urine. (chl.co.nz)
Laboratories3
- Hospital laboratories must make cystatin C available for clinical care to improve the safety and efficacy of medications that have narrow therapeutic windows. (lww.com)
- Cystatin C calibration has varied substantially across laboratories between 2006-when the original NHANES III (First Examination) cystatin C stored surplus sera study was conducted -and 2009-when the present measurements were conducted. (cdc.gov)
- Users need to consider the shift in laboratories when comparing cystatin C measurements from the Second Examination samples to measurements from the First Examination and other NHANES samples, conducted at different time points. (cdc.gov)
Proteins1
- Also, a number of the cystatin-like proteins have been shown to be devoid of inhibitory activity. (wikipedia.org)
Creatinine2
- Creatinine-cystatin C ratio and mortality in cancer patients: a retrospective cohort study. (nih.gov)
- Epidemiological studies show that high circulating cystatin C is associated with risk of cardiovascular disease (CVD), independent of creatinine-based renal function measurements. (scilifelab.se)
Chronic2
- February 27, 2009 - Serum cystatin C level and chronic kidney disease may have a link to incidence of age-related macular degeneration (AMD) that is independent of smoking and other risk factors, according to a population-based cohort study reported in the February issue of the Archives of Ophthalmology . (medscape.com)
- The goal of this study was to examine the associations of the serum cystatin C level and chronic kidney disease with the incidence and progression of AMD during 15 years of follow-up in 4926 participants of the Beaver Dam Eye Study. (medscape.com)
Concentration3
- Use the intensity and the standard curve to calculate the Cystatin C concentration in the samples. (arborassays.com)
- A decreased concentration of cystatin C in the blood circulation. (nih.gov)
- The purpose of the present study was to evaluate serum canine Cystatin-C (Cys-C) concentration for the prediction of renal function (RF) in dogs naturally infected with Babesia canis vogeli and Ehrlichia canis. (omu.edu.tr)
Cross-reactivity1
- No significant cross-reactivity or interference between Cystatin 6 (CST6) and analogues was observed. (biocheminfo.org)
Specificity1
- The Abs in the kit have high sensitivity and excellent specificity for detection of Cystatin 6 (CST6). (biocheminfo.org)
Marker2
- Various studies have reported about cystatin C as an easy and rapid assessable marker that can be used for accurate information on renal function impairment in humans lastly, but we have found only one study in dogs. (vin.com)
- A new marker of GFR, cystatin C, introduced in 1979, has been shown to be virtually uninfluenced by muscle mass. (lu.se)
Equation1
- Hans Pottel et al, Cystatin C-Based Equation to Estimate GFR without the Inclusion of Race and Sex, New England Journal of Medicine (2023). (medicalxpress.com)
Assays2
- The inter-assay coefficients of variation for the cystatin C, βTP and β2M assays were 4.0% (mean 0.698 mg/L), 5.7% (mean 0.594 mg/L) and 2.7% (mean 1.757 mg/L), respectively. (cdc.gov)
- Due to differences in laboratory calibration of assays, there are systematic differences in the cystatin C values from the First Examination and those from the Second Examination and other NHANES surveys. (cdc.gov)
Estimate1
- Hans Pottel, Ph.D., from KU Leuven Campus Kulak Kortrijk in Belgium, and colleagues used data from patients in Sweden to estimate the rescaling factor for cystatin C level in adults. (medicalxpress.com)
Clinical1
- Cystatin C could be a useful clinical tool to identify HIV-infected persons. (betalifesci.com)
Assay1
- Cystatin C measurements were conducted using the Siemens (formerly Dade Behring) N Latex Cystatin C assay, an automated particle-enhanced nepholometric assay (Siemens Diagnostics). (cdc.gov)
Chicken1
- Chicken cystatin quickly passed the membrane of MCF-10A neo T cells and inhibited cathepsin B when it was acylated with fatty acyl residues of 6-18 carbon atoms. (wikipedia.org)
Kidney function1
Level1
- It can also be calculated using your cystatin C level. (kidney.org)