• Other members include cystathionine gamma synthase, cystathionine beta lyase, and methionine gamma lyase. (wikipedia.org)
  • The crystal structure of cystathionine gamma-lyase (CGL) from yeast has been solved by molecular replacement at a resolution of 2.6 A. The molecule consists of 393 amino acid residues and one PLP moiety and is arranged in the crystal as a tetramer with D2 symmetry as in other related enzymes of the Cys-Met-metabolism PLP-dependent family like cystathionine beta-lyase (CBL). (rcsb.org)
  • Cystathionine gamma lyase also shows gamma-synthase activity depending on the concentrations of reactants present. (wikipedia.org)
  • In the gamma synthase mechanism, the gamma carbon is attacked by a sulfur nucleophile, resulting in the formation of a new sulfur-gamma carbon bond. (wikipedia.org)
  • CGLs from yeast or human are virtually identical at their active sites to cystathionine gamma-synthase (CGS) from E. coli. (rcsb.org)
  • Both CGLs and bacterial CGSs exhibit gamma-synthase and gamma-lyase activities depending on their position in the metabolic pathway and the available substrates. (rcsb.org)
  • Below are the list of possible Bifunctional cystathionine gamma-lyase/cysteine synthase products. (mybiosource.com)
  • Also known as Bifunctional cystathionine gamma-lyase/cysteine synthase (Beta-substituted Ala synthase 4;3) (ARAth-Bsas4;3) (L-cysteine desulfhydrase 1) (DES1) (O-acetylserine (thiol)-lyase) (OASTL) (Protein CS-LIKE). (mybiosource.com)
  • H2S is endogenously generated by cystathionine β-synthase (Cbs) and cystathionine γ-lyase (Cth). (nih.gov)
  • In the human body, H2S production is predominantly catalyzed by cystathionine-β-synthase (CBS) and cystathionine γ-lyase (CSE). (nih.gov)
  • Hydrogen sulfide (H 2 S), a proangiogenic, pro-alveolarization, and anti-asthmatic gasotransmitter is synthesized by cystathionine-γ-lyase (CSE), cystathionine-β-synthase (CBS), and 3- mercaptopyruvate sulfur transferase (3MST). (frontiersin.org)
  • In mammals, H 2 S is mainly produced from L-cysteine by three enzymes: cystathionine γ-lyase (CSE), cystathionine β-synthase (CBS), and 3-mercaptopyruvate sulfur transferase (3MST) ( 19 , 20 ). (frontiersin.org)
  • Cystathionine synthase-like enzymes, such as cystalysin, methionine gamma-lyase (MGL), and cysteine desulphurase (IscS) [ PMID: 17014820 ]. (ebi.ac.uk)
  • Cystathionine β-synthase (CBS) is a multidomainal enzyme with three modules and a heme domain. (bionity.com)
  • The human enzyme cystathionine β-synthase is a tetramer and comprises 551 amino acids with a subunit molecular weight of 63 kDa. (bionity.com)
  • The C-terminal domain of cystathionine β-synthase regulates its activity via both intrasteric and allosteric effects and is important for maintaining the tetrameric state of the protein [2] . (bionity.com)
  • Homocysteine is an intermediate in methionine synthesis in Aspergillus nidulans , but it can also be converted to cysteine by the reverse transsulfuration pathway involving cystathionine β-synthase (CBS) and cystathionine γ-lyase (CGL). (springer.com)
  • Braunstein AE, Goryachenkova EV, Tolosa EA, Willhardt IH, Yefremova LL (1971) Specificity and some other properties of liver serine sulphhydrase: evidence for its identity with cystathionine-synthase. (springer.com)
  • H 2 S can be generated endogenously from L-cysteine by multiple enzymes, including cystathionine gamma-lyase, cystathionine beta-synthase, and 3-mercaptopyruvate sulfurtransferase in combination with cysteine aminotransferase. (hindawi.com)
  • All mammalian organ systems produce hydrogen sulfide through three key enzymes: cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3MST). (podiatrytoday.com)
  • Two H 2 S producing enzymes are part of the cysteine biosynthesis pathway: cystathionine gamma lyase (CSE) and cystathionine beta synthase (CBS). (hindawi.com)
  • Also, certain hub genes were highlighted after constructed and analyzed the protein-protein interaction network, including α-2A adrenergic receptor, cytochrome P450 subfamily IIR polypeptide, Cystathionine β-synthase, nucleotide-binding oligomerization domain-containing, erythropoietin-producing hepatocellular receptor A2 and adrenomedullin, which may be related with BC prognosis. (lww.com)
  • Cystathionine-β-synthase , also known as CBS , is an enzyme ( EC 4.2.1.22 ) that in humans is encoded by the CBS gene . (wikidoc.org)
  • Cystathionine beta synthase catalyzes the upper reaction and cystathionine gamma-lyase catalyzes the lower reaction. (wikidoc.org)
  • Lastly, we found DATS could increase the expressions of cystathionine gamma-lyase (CSE) and cystathionine beta-synthase (CBS), the major H2S-producing enzymes. (unboundmedicine.com)
  • H2S is produced from cysteine, involving the enzymes cystathionine beta-synthase and cystathionine gamma-lyase (CSE). (uzh.ch)
  • Dexamethasone also reduced the LPS-evoked rise in plasma hydrogen sulphide (H 2 S) concentration, liver H 2 S synthesizing activity and expression of cystathionine γ lyase (CSE) and inducible nitric oxide synthase (iNOS). (wiley.com)
  • Hydrogen sulfide (H2S) is synthesized by 2 enzymes, cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE). (strath.ac.uk)
  • Cystathionine gamma-lyase is a member of the Cys/Met metabolism PLP-dependent enzymes family. (wikipedia.org)
  • CTH (Cystathionine Gamma-Lyase) is a Protein Coding gene. (genecards.org)
  • Nickel also repressed the mRNA and protein expression of cystathionine gamma-lyase (CSE, a H 2 S-generating gene) and blocked the endogenous production of H 2 S. Exogenously applied NaHS (a H 2 S donor) had no effect on nickel-induced cell viability but significantly attenuated nickel-stimulated cell migration and the expression of αSMA and fibronectin. (nih.gov)
  • As a result, abnormally high concentrations of cystathionine appear in the urine. (britannica.com)
  • Methods: We measured concentrations of cystathionine as well as 22 other amino acids in fasting plasma and 24-h urine samples of nine early-stage HD patients and nine age, sex, and body mass index matched controls. (plos.org)
  • Results: There were no significant differences in the plasma or urine concentrations of cystathionine or any other amino acid between HD patients and controls. (plos.org)
  • Conclusion: We found no evidence for changes in plasma or urine concentrations of cystathionine in early-stage HD patients. (plos.org)
  • The most striking feature was marked accumulation of Met and cystathionine in serum/urine/kidney samples of Cbs(-/-) and Cth(-/-) mice, respectively. (jove.com)
  • We therefore hypothesized that blood and urine cystathionine levels may be increased in HD patients and that this increase might correlate with disease progression. (plos.org)
  • 4 Paralleling findings in individuals with inactivating mutations of the cystathionine γ-lyase (CTH) gene which encodes CSE, a depletion of CSE might result in elevated levels of cystathionine in both blood and urine. (plos.org)
  • 6 Therefore, we hypothesized that the levels of cystathionine in both blood and urine may be increased in patients with HD compared to matched controls and that this increase might correlate with disease progression. (plos.org)
  • Expression of cystathionine-γ-lyase, the rate-limiting enzyme of the transsulfuration pathway, was significantly increased following incubation of the cells with gliotoxins. (ucd.ie)
  • It is concluded that glutathione depletion causes a JNK- and p38MAPK-mediated increase in expression of cystathionine-γ-lyase that promotes flux through the transsulfuration pathway to compensate for loss of glutathione in C6 glioma cells. (ucd.ie)
  • Alexander FW, Sandmeier E, Mehta PK, Christen P (1994) Evolutionary relationships among pyridoxal-5′-phosphate-dependent enzymes: regio-specific alpha, beta and gamma families. (springer.com)
  • Cardiac nitrotyrosine formation increased while expression of the endogenous hydrogen sulphide (H 2 S)-producing enzyme cystathionine γ-lyase (CSE) decreased. (pubmedcentralcanada.ca)
  • Common aspects of these studies are the reported dysregulation of tissue cystathionine-gamma-lyase (CSE) expression (the major hydrogen sulfide producing enzyme in the periphery) and the use of D,L-propargylglycine (PAG) to inhibit CSE activity. (otago.ac.nz)
  • These findings suggest that hydrogen sulphide protects the kidney against ischaemia/reperfusion injury and that the increase in expression of the enzyme cystathionine gamma-lyase during ischaemia/reperfusion injury may be one of many endogenous mechanisms to limit renal ischaemia/reperfusion injury. (westminster.ac.uk)
  • Incubation of C6 cells with diethylmaleate for 3 h led to a significant reduction in glutathione (63%), whereas expression of cystathionine-γ-lyase was increased by 1.5-fold. (ucd.ie)
  • Co-incubation of C6 cells with diethylmaleate and the JNK-inhibitor, SP600125, abolished the increase in expression of cystathionine-γ-lyase that had been observed in the presence of diethylmaleate alone. (ucd.ie)
  • The aortic eNOS- and cystathionine-γ-lyase(CSE) proteins expression of the CCM-treated rats were also higher than in controls. (scielo.br)
  • Chronic exposure to high glucose induces the expression of cystathionine gamma-lyase (CSE), a hydrogen sulfide-producing enzyme, in pancreatic beta-cells, thereby suppressing apoptosis. (jove.com)
  • Cystathionine gamma-lyase (CSE) is the major enzyme responsible for the endogenous production of H2S in pancreatic β cells and liver cells, which are two key types of cells to regulate glucose level. (lakeheadu.ca)
  • Western blot analysis of the kidney taken at the end of the experiment demonstrated that cystathionine gamma-lyase, the enzyme responsible for generating hydrogen sulphide in the cardiovascular system, is expressed in the normal kidney and is significantly increased after ischaemia/reperfusion injury. (westminster.ac.uk)
  • We reported a substantial depletion of cystathionine γ-lyase (CSE), the primary biosynthetic enzyme for the amino acid cysteine, in HD ( 8 ). (pnas.org)
  • 2003. Activation of Peroxisome Proliferator-Activated Receptor Gamma and Retinoid X Receptor Results in Net Depletion of Cellular Cholesteryl Esters in Macrophages Exposed to Oxidized Lipoproteins. (uwo.ca)
  • Background: Recently a profound depletion of cystathionine γ-lyase (CSE), the principal enzyme involved in the generation of cysteine from cystathionine, was shown in Huntington disease (HD) patients and several transgenic HD mouse models. (plos.org)
  • Recently a substantial depletion of cystathionine γ-lyase (CSE), the principal enzyme involved in the generation of cysteine from cystathionine, was shown in HD. (plos.org)
  • Our aim was to study the effect of caffeic acid phenethyl ester (CAPE) on iNOS and cystathionine gamma-lyase (CSE) of hepatic fibrosis rat, and discuss the anti-hepatic fibrosis mechanism of caffeic acid phenethyl ester. (medscimonit.com)
  • It is now becoming increasingly apparent that hydrogen sulphide (H 2 S) formed from cysteine by the enzyme, cystathionine γ lyase (CSE), exerts complex effects on inflammation. (wiley.com)
  • Cystathionine gamma-lyase also catalyses the following elimination reactions: L-homoserine to form H2O, NH3 and 2-oxobutanoate L-cystine, producing thiocysteine, pyruvate and NH3 L-cysteine producing pyruvate, NH3 and H2S In some bacteria and mammals, including humans, this enzyme takes part in generating hydrogen sulfide. (wikipedia.org)
  • This enzymatic activity accounts for the mitogenic properties of PLC-gamma 1. (jove.com)
  • The amino group on cystathionine is deprotonated and undergoes a nucleophilic attack of the internal aldimine. (wikipedia.org)
  • This group of enzymes has a glutamate (E333 in yeast CGL) which binds to the distal group of cystathionine (CTT) or the amino group of cysteine. (rcsb.org)
  • To reform the aldimine, this lone pair pushes back down, cleaving the sulfur-gamma carbon bond, resulting in the release of cysteine. (wikipedia.org)
  • Gene Ontology (GO) annotations related to this gene include calmodulin binding and carbon-sulfur lyase activity . (genecards.org)
  • Hydrogen sulfide (H2S) is an endogenous inflammatory mediator produced by the activity of cystathionine γ-lyase (CSE) in mammals. (otago.ac.nz)
  • lyase (CSE) plays a pro- or anti-inflammatory role in caerulein-induced pancreatitis. (jove.com)
  • Fidel S, Doonan JH, Morris NR (1988) Aspergillus nidulans contains a single actin gene which has unique intron locations and encodes a gamma-actin. (springer.com)